Terrestrial paleoclimate archives such as lake sediments are essential for our understanding of the continental climate system and for the modeling of future climate scenarios. However, quantitative ...proxies for the determination of paleotemperatures are sparse. The relative abundances of certain bacterial lipids, i.e., branched glycerol dialkyl glycerol tetraethers (brGDGTs), respond to changes in environmental temperature, and thus have great potential for climate reconstruction. Their application to lake deposits, however, is hampered by the lack of fundamental knowledge on the ecology of brGDGT-producing microbes in lakes. Here, we show that brGDGTs are synthesized by multiple groups of bacteria thriving under contrasting redox regimes in a deep meromictic Swiss lake (Lake Lugano). This niche partitioning is evidenced by highly distinct brGDGT inventories in oxic vs. anoxic watermasses, and corresponding vertical patterns in bacterial 16S rRNA gene abundances, implying that sedimentary brGDGT records are affected by temperature-independent changes in the community composition of their microbial producers. Furthermore, the stable carbon isotope composition (δ13C) of brGDGTs in Lake Lugano and 34 other (peri-)Alpine lakes attests to the widespread heterotrophic incorporation of 13C-depleted, methane-derived biomass at the redox transition zone of mesotrophic to eutrophic lake systems. The brGDGTs produced under such hypoxic/methanotrophic conditions reflect near-bottom water temperatures, and are characterized by comparatively low δ13C values. Depending on climate zone and water depth, lake sediment archives predominated by deeper water/low-13C brGDGTs may provide more reliable records of climate variability than those where brGDGTs derive from terrestrial and/or aquatic sources with distinct temperature imprints.
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BFBNIB, NMLJ, NUK, PNG, SAZU, UL, UM, UPUK
Lakes are large sources of methane, held to be responsible for 18% of the radiative forcing, to the atmosphere. Periods of lake overturn (during fall/winter) are short and therefore difficult to ...capture with field campaigns but potentially one of the most important periods for methane emissions. We studied methane emissions using four different methods, including eddy covariance measurements, floating chambers, anchored funnels, and boundary model calculations. Whereas the first three methods agreed rather well, boundary model estimates were 5–30 times lower leading to a strong underestimation of methane fluxes from aquatic systems. These results show the importance of ebullition as the most important flux pathway and the need for continuous measurements with a large footprint covering also shallow parts of lakes. Although fluxes were high, on average 4 mmol m–2 d–1 during the overturn period, water column microbial methane oxidation removed 75% of the methane and only 25% of potential emissions were released to the atmosphere. Hence, this study illustrates second the importance of considering methane oxidation when estimating the flux of methane from lakes during overturn periods.
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IJS, KILJ, NUK, PNG, UL, UM
Mitochondria are specialized eukaryotic organelles that have a dedicated function in oxygen respiration and energy production. They evolved about 2 billion years ago from a free-living bacterial ...ancestor (probably an alphaproteobacterium), in a process known as endosymbiosis
. Many unicellular eukaryotes have since adapted to life in anoxic habitats and their mitochondria have undergone further reductive evolution
. As a result, obligate anaerobic eukaryotes with mitochondrial remnants derive their energy mostly from fermentation
. Here we describe 'Candidatus Azoamicus ciliaticola', which is an obligate endosymbiont of an anaerobic ciliate and has a dedicated role in respiration and providing energy for its eukaryotic host. 'Candidatus A. ciliaticola' contains a highly reduced 0.29-Mb genome that encodes core genes for central information processing, the electron transport chain, a truncated tricarboxylic acid cycle, ATP generation and iron-sulfur cluster biosynthesis. The genome encodes a respiratory denitrification pathway instead of aerobic terminal oxidases, which enables its host to breathe nitrate instead of oxygen. 'Candidatus A. ciliaticola' and its ciliate host represent an example of a symbiosis that is based on the transfer of energy in the form of ATP, rather than nutrition. This discovery raises the possibility that eukaryotes with mitochondrial remnants may secondarily acquire energy-providing endosymbionts to complement or replace functions of their mitochondria.
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GEOZS, IJS, IMTLJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBMB, UL, UM, UPUK, ZAGLJ
Lakes are a natural source of methane to the atmosphere and contribute significantly to total emissions compared to the oceans. Controls on methane emissions from lake surfaces, particularly biotic ...processes within anoxic hypolimnia, are only partially understood. Here we investigated biological methane oxidation in the water column of the seasonally stratified Lake Rotsee. A zone of methane oxidation extending from the oxic/anoxic interface into anoxic waters was identified by chemical profiling of oxygen, methane and δ13C of methane. Incubation experiments with 13C-methane yielded highest oxidation rates within the oxycline, and comparable rates were measured in anoxic waters. Despite predominantly anoxic conditions within the zone of methane oxidation, known groups of anaerobic methanotrophic archaea were conspicuously absent. Instead, aerobic gammaproteobacterial methanotrophs were identified as the active methane oxidizers. In addition, continuous oxidation and maximum rates always occurred under light conditions. These findings, along with the detection of chlorophyll a, suggest that aerobic methane oxidation is tightly coupled to light-dependent photosynthetic oxygen production both at the oxycline and in the anoxic bottom layer. It is likely that this interaction between oxygenic phototrophs and aerobic methanotrophs represents a widespread mechanism by which methane is oxidized in lake water, thus diminishing its release into the atmosphere.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Redox dynamics of manganese (Mn) were studied in the sediment of Lake Zurich using precise sediment core age models, monthly long-term oxygen (O2) monitoring data of the water column (1936–2010) and ...high-resolution XRF core scanning. The age models were based on bi-annual lamination and calcite precipitation cycles. If present, Mn exhibits distinct maxima, which coincide with the annual maximum deep-water O2 concentrations in spring according to the monitoring data. In contrast, the iron (Fe) signal is mainly the result of calcite dilution, as indicated by a strong negative correlation between Fe and calcium (Ca) XRF data. The Mn/Fe ratio in the core from the maximum lake depth (ZH10-15, 137m) revealed a moderate correlation with O2 measurements in the lake bottom water confirming the successful application of the Mn/Fe ratio to semi-quantitatively reconstruct bottom water oxygenation in the lake. Mostly low ratios were observed between 1895 and the mid-1960s as a result of eutrophication. However, geochemical focusing and sedimentological factors can reduce the applicability of the Mn/Fe ratio in reconstructing O2 concentrations in the bottom water of lakes.
•Seasonal resolved Mn and Fe records since 1895•Mn/Fe ratio correlated with oxygen monitoring data•Semi-quantitative bottom water O2 concentration reconstructions•Geochemical and sedimentological factors reduce applicability of this proxy
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Colonization of new ecological niches has triggered large adaptive radiations. Although some lineages have made use of such opportunities, not all do so. The factors causing this variation among ...lineages are largely unknown. Here, we show that deficiency in docosahexaenoic acid (DHA), an essential ω-3 fatty acid, can constrain freshwater colonization by marine fishes. Our genomic analyses revealed multiple independent duplications of the fatty acid desaturase gene
in stickleback lineages that subsequently colonized and radiated in freshwater habitats, but not in close relatives that failed to colonize. Transgenic manipulation of
in marine stickleback increased their ability to synthesize DHA and survive on DHA-deficient diets. Multiple freshwater ray-finned fishes also show a convergent increase in
copies, indicating its key role in freshwater colonization.
Branched glycerol dialkyl glycerol tetraethers (brGDGTs) are common in lake sediments, where they are frequently employed to infer mean annual air temperatures (MAAT) or air temperatures of months ...above freezing (MAF) using the MBT'5Me lipid paleothermometer. The reliable reconstruction of such temperatures, however, requires robust calibration functions. Here, we investigated brGDGT distributions in surface sediments from 41 freshwater lakes located along an altitudinal gradient across the Alps (Central Europe) and spanning a MAAT range from 1.3 to 12.9 °C. Linear regression analysis demonstrates that fractional abundances of brGDGTs are strongly correlated with MAAT and MAF allowing to establish regional MBT'5Me-based transfer functions: MAAT (°C) = −2.19 + 31.91 × MBT'5Me (r2 = 0.72; RMSE = 1.51 °C) and MAF (°C) = 4.81 + 15.64 × MBT'5Me (r2 = 0.64, RMSE = 0.92 °C). Stepwise forward selection yielded the following, alternate temperature calibrations: MAAT (°C) = 7.11 + 67.66 × Ib – 13.54 × IIIa (r2 = 0.72; RMSE = 1.47 °C) and MAF (°C) = 5.19 + 16.22 × Ia (r2 = 0.66; RMSE = 0.98 °C). Our results demonstrate that the above calibration functions allow precise temperature reconstructions using lacustrine sediment records. However, our data also show that high-altitude lakes are more prone to warm bias and that lakes characterized by comparatively high abundances of 6-methyl brGDGTs show aberrant behaviors with temperature offsets up to ∼7 °C. Determining the IR6Me, as an independent control complementary to the MBT'5Me, is thus essential to validate the robustness of brGDGT-based temperature reconstructions of past climates. A cut-off value of 0.50 for the IR6Me is here proposed, after which MBT'5Me-reconstructed MAATs from lacustrine archives should be regarded as unreliable.
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•The methylation of brGDGTs is affected strongly by mean annual air temperature.•Generation of an MBT'5Me-based temperature calibration for Central European lakes.•Lakes with IR6Me values >0.50 appear unsuited for temperature reconstructions.•Holomictic high-elevation lakes show a more pronounced response to seasonality.•Sedimentary 5/6-methyl brGDGT IIIa" are lacking in warm biased lakes.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Symbiotic N
-fixing microorganisms have a crucial role in the assimilation of nitrogen by eukaryotes in nitrogen-limited environments
. Particularly among land plants, N
-fixing symbionts occur in a ...variety of distantly related plant lineages and often involve an intimate association between host and symbiont
. Descriptions of such intimate symbioses are lacking for seagrasses, which evolved around 100 million years ago from terrestrial flowering plants that migrated back to the sea
. Here we describe an N
-fixing symbiont, 'Candidatus Celerinatantimonas neptuna', that lives inside seagrass root tissue, where it provides ammonia and amino acids to its host in exchange for sugars. As such, this symbiosis is reminiscent of terrestrial N
-fixing plant symbioses. The symbiosis between Ca. C. neptuna and its host Posidonia oceanica enables highly productive seagrass meadows to thrive in the nitrogen-limited Mediterranean Sea. Relatives of Ca. C. neptuna occur worldwide in coastal ecosystems, in which they may form similar symbioses with other seagrasses and saltmarsh plants. Just like N
-fixing microorganisms might have aided the colonization of nitrogen-poor soils by early land plants
, the ancestors of Ca. C. neptuna and its relatives probably enabled flowering plants to invade nitrogen-poor marine habitats, where they formed extremely efficient blue carbon ecosystems
.
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GEOZS, IJS, IMTLJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBMB, UL, UM, UPUK, ZAGLJ
Methane-oxidizing bacteria represent a major biological sink for methane and are thus Earth's natural protection against this potent greenhouse gas. Here we show that in two stratified freshwater ...lakes a substantial part of upward-diffusing methane was oxidized by filamentous gamma-proteobacteria related to Crenothrix polyspora. These filamentous bacteria have been known as contaminants of drinking water supplies since 1870, but their role in the environmental methane removal has remained unclear. While oxidizing methane, these organisms were assigned an 'unusual' methane monooxygenase (MMO), which was only distantly related to 'classical' MMO of gamma-proteobacterial methanotrophs. We now correct this assignment and show that Crenothrix encode a typical gamma-proteobacterial PmoA. Stable isotope labeling in combination swith single-cell imaging mass spectrometry revealed methane-dependent growth of the lacustrine Crenothrix with oxygen as well as under oxygen-deficient conditions. Crenothrix genomes encoded pathways for the respiration of oxygen as well as for the reduction of nitrate to N
O. The observed abundance and planktonic growth of Crenothrix suggest that these methanotrophs can act as a relevant biological sink for methane in stratified lakes and should be considered in the context of environmental removal of methane.
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Freshwater lakes represent large methane sources that, in contrast to the Ocean, significantly contribute to non-anthropogenic methane emissions to the atmosphere. Particularly mixed lakes are major ...methane emitters, while permanently and seasonally stratified lakes with anoxic bottom waters are often characterized by strongly reduced methane emissions. The causes for this reduced methane flux from anoxic lake waters are not fully understood. Here we identified the microorganisms and processes responsible for the near complete consumption of methane in the anoxic waters of a permanently stratified lake, Lago di Cadagno. Interestingly, known anaerobic methanotrophs could not be detected in these waters. Instead, we found abundant gamma-proteobacterial aerobic methane-oxidizing bacteria active in the anoxic waters. In vitro incubations revealed that, among all the tested potential electron acceptors, only the addition of oxygen enhanced the rates of methane oxidation. An equally pronounced stimulation was also observed when the anoxic water samples were incubated in the light. Our combined results from molecular, biogeochemical and single-cell analyses indicate that methane removal at the anoxic chemocline of Lago di Cadagno is due to true aerobic oxidation of methane fuelled by in situ oxygen production by photosynthetic algae. A similar mechanism could be active in seasonally stratified lakes and marine basins such as the Black Sea, where light penetrates to the anoxic chemocline. Given the widespread occurrence of seasonally stratified anoxic lakes, aerobic methane oxidation coupled to oxygenic photosynthesis might have an important but so far neglected role in methane emissions from lakes.
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