Dalmatian pyrethrum (
(Trevir.) Sch. Bip.), a plant species endemic to the east Adriatic coast, is used worldwide for production of the organic insecticide, pyrethrin. Most studies concerning ...Dalmatian pyrethrum have focused on its morphological and biochemical traits relevant for breeding. However, little is known about the chromosomal evolution and genome organization of this species. Our study aims are to identify, classify, and characterize repetitive DNA in the
genome using clustering analysis of a low coverage genomic dataset. Repetitive DNA represents about 71.63% of the genome.
exhibits linked 5S and 35S rDNA configuration (L-type). FISH reveals amplification of interstitial telomeric repeats (ITRs) in
. Of the three newly identified satellite DNA families, TcSAT1 and TcSAT2 are located subterminally on most of
chromosomes, while TcSAT3 family is located intercalary within the longer arm of two chromosome pairs. FISH reveals high levels of polymorphism of the TcSAT1 and TcSAT2 sites by comparative screening of 28 individuals. TcSAT2 is more variable than TcSAT1 regarding the number and position of FISH signals. Altogether, our data highlights the dynamic nature of DNA sequences associated with subtelomeres in
and suggests that subtelomeres represent one of the most dynamic and rapidly evolving regions in eukaryotic genomes.
Načini ponavljanja nastavnog sadržaja stalno se mijenjaju, primjenjuju se nove metode koje nerijetko daju bolje rezultate na kraju poučavanja. Cilj istraživanja je utvrditi kako ponavljanje primjenom ...interaktivnih lekcija u online okruženju može utjecati na razumijevanje i povezivanje nastavnih sadržaja biologije. Istraživanje je provedeno 2022. godine, a vezano je uz nastavne teme Hrana kao izvor energije i Prilagodbe živih bića na život u vodi te Svojstva vode kao tema baziranu na terenskom istraživanju. Nastavne teme su odabrane uz dva biološka koncepta - koncept A (Energetski učinci prehrane živih bića) i koncept B (Prilagodbe živih bića kao posljedice evolucije) te uz poučavanje primjenom simulacija terenskih istraživanja. Uzorak je sačinjavalo šest razrednih odjeljenja petih razreda, koji su ponavljali nastavne sadržaje u online obliku uz pomoć Moodle lekcija platforme BUBO (na sustavu MoD, SRCE). Na kontrolnom uzorku primijenjeno je klasično ponavljanje. Svi učenici u istraživanju poučavani su uz pomoć istih nastavnih materijala. Lekcije za ponavljanje su izrađene na osnovu materijala koji su služili za poučavanje i učenje pojedine teme. Nakon ponavljanja, učenici su pisali završnu provjeru znanja koja je sadržavala po jedno pitanje iz svake teme s po tri čestice, od kojih je svaka čestica provjeravala jednu od kognitivnih razina znanja (reprodukcija, razumijevanje i primjena znanja, rješavanje problema). Uspješnost učenja uz interaktivno online ponavljanje u obliku Moodle lekcije prikazana je na osnovi praćenja ostvarenosti ishoda tijekom rješavanja zadataka u sklopu lekcije i na osnovi rezultata rješavanja završne pisane provjere. Za prikupljanje informacija o dojmovima učenja uz lekcije za ponavljanje korišten je anketni upitnik s trostupanjskom Likertovom skalom, kojim su učenici procjenjivali težinu pitanja, zanimljivost lekcije, kvalitetu uputa i kvalitetu sadržaja. Učenici većinom procjenjuju kako im je učenje uz pomoć lekcija praktično, jasno i jednostavnije od klasičnog ponavljanja. U velikoj većini slažu se da je ovaj način ponavljanja zanimljiv te da bi bilo dobro češće učiti na ovaj način, pri čemu navode kako je lakše ponavljati kada su postavljena pitanja koja ih vode u učenju, jer na taj način lakše uočavaju bitne sadržaje i poveznice između sadržaja.
Copper(II) complexes with N-alkyliminodiacetamide derivatives (Rimda), Cu(Rimda)2(NO3)2 (R = Me, Et, Pr, Bu) were studied. The complexes were characterized by IR spectroscopy, thermal analysis ...(TG/DTA) and X-ray structural analysis. The complexes were tested on bacteria (Escherichia coli and Staphylococcus aureus), fungi (Candida albicans) and Lemna minor L.
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•Copper(II) complexes with iminodiacetamide derivatives were studied.•The complexes were characterized by X-ray crystallography.•O,N,O'-tridentate iminodiacetamide ligands are bound in fac-configuration.•The complexes were studied by DFT structural and vibrational calculations.•The complexes were tested on bacteria, fungi and Lemna minor L.
By the reactions of copper(II) nitrate with N-alkyliminodiacetamide derivatives, RN(CH2CONH2)2 (Rimda; R = Me (methyl), Et (ethyl), Pr (propyl) and Bu (butyl)), four new water-soluble copper(II) complexes were synthesized, namely Cu(Meimda)2(NO3)2 (1), Cu(Etimda)2(NO3)2 (2), Cu(Primda)2(NO3)2 (3) and Cu(Buimda)2(NO3)2 (4). The complexes were characterized by infrared and UV/Vis spectroscopy and thermal analysis (TG/DTA). The molecular and crystal structures of 1 and 4 were determined by X-ray crystallography. The octahedral coordination environment around the copper(II) ion consists of two O,N,O'-tridentate N-alkyliminodiacetamide ligands and the complexes are trans-isomers with fac-configuration of the chelators. Various hydrogen bond ring motifs e. g. R24(8), R44(12), R21(5), R22(8) and R34(16) were established in the crystal structures of 1 and 4. The molecular geometry and infrared spectra of the complex cations Cu(Meimda)22+, Cu(Etimda)22+, Cu(Primda)22+ and Cu(Buimda)22+ were also studied by DFT calculations using BP86, B3LYP and B97-D computational models. Their calculated spectra and molecular geometries agree very well with the experimental results. The complexes were tested for possible antibacterial and antifungal activity on bacteria (Escherichia coli and Staphylococcus aureus) and fungi (Candida albicans), respectively, and showed low or negligible activity. The complexes also showed relatively high toxic effects on Lemna minor L. (duckweed), a model aquatic organism used to test the toxic effects of metal ions.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
4.
FILOZOFSKI ŽIVOT Mudri, Nebojša; Hanžek, Ljudevit; Marasović, Tina ...
Filozofska istraživanja,
05/2011, Volume:
30, Issue:
4
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