The study of stool microbiota has taken great relevance in the last years, given its role in the maintenance of the intestinal metabolic, physiological, and immunological homeostasis, as well as, its ...effect over HIV biomarkers levels such as CD4/CD8 ratio, high sensitivity C-Reactive Protein (hs-CRP), related to poor outcomes (rapid progression to AIDS). Several efforts have been made to characterize the gut microbiome. In HIV infection, most of the studies report the presence of a dysbiotic pattern; however, few of them have made an approach in elderly HIV-positive subjects despite the fact that nowadays this subgroup is rising. In this study, we compared the composition of faecal microbiota, Short Chain Fatty Acids (SCFAs), and systemic biomarkers between elderly HIV-positive and HIV-negative subjects.
A cross-sectional study with 18 HIV-negative controls and 20 HIV-positive patients. The quantification of Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria, Lactobacillus, Enterobacteriaceae, Bifidobacterium, Escherichia coli, Clostridium leptum, Clostridium coccoides was performed in faecal samples by qPCR. The analysis was performed by calculating the ΔCq of each microorganism using 16S rDNA as a reference gene. Faecal SCFAs were measured by HPLC. The hs-CRP and sCD14 were performed by ELISA.
An increase in the Firmicutes/Bacteroidetes ratio, coupled with a significant increase in the proteobacteria phylum was detected in HIV-positive subjects. In contrast, a decrease in the Clostridium leptum group was observed. Nevertheless, these elderly HIV-positive patients showed higher levels of total SCFAs mainly by an augmented propionic acid values, compared to HIV-negative subjects. Whereas high levels of hs-CRP were positively correlated with sCD14 in the HIV-positive group.
Alterations in bacterial communities reveals a dysbiotic state related to an unbalance of faecal SCFAs. Therefore, these intestinal conditions might drive an increase of poor prognostic biomarkers in elderly HIV-positive subjects.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Grain size and SEM analyses suggest the presence of Cretaceous windblown desert sands in coeval shallow marine environments. Size distributions and microtexture data allowed us to infer a climate ...change to more arid conditions in the Iberian Basin during the mid‐Cretaceous. The grain size of the sands in the late Aptian to early Cenomanian shallow‐marine deposits in the western sub‐basins of the Maestrazgo Basin (Teruel, Spain) is almost exclusively in the range between 1.5 and 3 Φ (0.35–0.125 mm), reflecting a prolonged or at least recurrent preselection of aeolian sands. The palaeolatitude of 25°N showed a change from a warm humid climate during the Lower Cretaceous to an arid desert climate in the eastern sector of Iberia during the late Aptian–early Cenomanian. Winds supplied abundant desert sand to the estuarine and deltaic sedimentary environments where it was worked up in sandy sub‐ and intertidal facies with a striking absence of mud in cross‐bedded sets which otherwise clearly reflect the influence of a semi‐diurnal tidal system.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of
or
species. We collected fluconazole, ...itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171
, 215
, 4,418
species complex, 157
(
), 676
(
), 298
(
), 911
, 3,691
species complex, 36
, 110
, 1,854
, 244
, 1,409
, 389
, 130
, 233
, and 302
complex isolates. SYO/Etest MICs for 282 confirmed non-wild-type (non-WT) isolates were included:
(
),
and
(
),
(
), and
and
overexpression (
and
, respectively). Interlaboratory modal agreement was superior by SYO for yeast species and by the Etest for
spp. Distributions fulfilling CLSI criteria for epidemiological cutoff value (ECV) definition were pooled, and we proposed SYO ECVs for
and 9 yeast and 3
species and Etest ECVs for 5 yeast and 4
species. The posaconazole SYO ECV of 0.06 µg/ml for
and the Etest itraconazole ECV of 2 µg/ml for
were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as, overall, the SYO appears to perform better for susceptibility testing of yeast species and the Etest appears to perform better for susceptibility testing of
spp. Further evaluations should be conducted with more
mutants.
Introduction
Measuring attitudes towards disability is meant to assess which interventions are most likely to create changes in population attitudes. Physical activities, such as Traditional Sports ...Games, are an excellent methodology to fight against the stigma of disabled people. Thus, the main aim of this study was to validate the Chedoke-McMaster Attitudes towards Children with Handicaps Scale (CATCH) adapted to a physical activity environment.
Methods
Additionally, we implemented this process in a combined way, 7 languages and 2 versions (adult and youth) at the same time.
Results
The results showed that the CATCH-TSG scale provides the scientific community with a valid and reliable tool for professionals who want to assess the change in attitudes towards people with disabilities after receiving a psychoeducational intervention that includes physical activity (TSG).
Discussion
Researchers will compare respective results from different countries and ages using different versions of the scale, jointly validated.
•Retention characteristics of four commercial ionic liquid columns were first evaluated.•They are highly efficient and polar columns with important acid–base behavior.•All types of solute-stationary ...phase interactions are possible in these columns.•Temperature mainly decreased their capacity of H-acceptor interactions with solutes.•These IL columns provide a different selectivity over non-ionic polar stationary phases.
For the first time, four commercial ionic liquid columns (SLB-IL59, SLB-IL76, SLB-IL82 and SLB-IL100) for gas chromatography have been comprehensively evaluated in terms of efficiency, polarity and solvation properties. Grob tests and McReynolds constants showed that they were all high-efficiency columns of high polarity, but with low inertness to compounds with hydrogen bonding capabilities. The solvation parameter model was used to characterize the solvation interactions of the four columns in the 80–160°C temperature range. Results revealed that all the ionic liquids studied can be considered moderately hydrogen-bond acid and highly cohesive stationary phases, on which the dominant contributions to retention were the dipolar-type and hydrogen-bond base interactions, while π–π and n–π interactions were barely significant. The SLB-IL59 column provided the best separation of homologs, while the SLB-IL76 and SLB-IL100 columns had the most basic and the most acidic phases, respectively. A principal component analysis for the commonly used stationary phases in capillary GC showed that these commercial ionic liquid columns fill an empty area of the available selectivity space, which clearly enhances the separation capacity of this technique.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
We have investigated in Sinorhizobium fredii HH103-1 (=HH103 Str super(r)) the influence of the nolR gene on the production of three different bacterial symbiotic signals: Nod factors, signal ...responsive (SR) proteins, and exopolysac-charide (EPS). The presence of multiple copies of nolR (in plasmid pMUS675) repressed the transcription of all the flavonoid-inducible genes analyzed: nodA, nodD1, nolO, nolX, noeL, rhcJ, hesB, and y4pF. Inactivation of nolR (mutant SVQ517) or its overexpression (presence of pMUS675) altered the amount of Nod factors detected. Mutant SVQ517 produced Nod factors carrying N-methyl residues at the nonreducing N-acetyl-glucosamine, which never have been detected in S. fredii HH103. Plasmid pMUS675 increased the amounts of EPS produced by HH103-1 and SVQ517. The flavonoid genistein repressed EPS production of HH103-1 and SVQ517 but the presence of pMUS675 reduced this repression. The presence of plasmid pMUS675 clearly decreased the secretion of SR proteins. Inactivation, or overexpression, of nolR decreased the capacity of HH103 to nodulate Glycine max. However, HH103-1 and SVQ517 carrying plasmid pMUS675 showed enhanced nodulation capacity with Vigna unguiculata. The nolR gene was positively identified in all S. fredii strains investigated, S. xinjiangense CCBAU110, and S. saheli USDA4102. Apparently, S. teranga USDA4101 does not contain this gene.
The selection of a suitable organic amendment for recovery of semi-arid soils degraded by mining is key to the success of an ecological restoration. The aim of this research is to study the ...short-term responses of physicochemical, biochemical and biological properties, as well as the changes of a soil bacterial community at the genus level after application of five types of organic amendments in a limestone quarry in Almería (SE, Spain). The relationship among bacterial taxa with biochemical and physicochemical properties and priming effect from restored soils was also analysed. Six months after the application of organic amendments, the values of different soil status, such as total organic carbon, total nitrogen, assimilable phosphorus and labile organic matter forms (carbohydrates and polyphenols), basal respiration (BR) and enzymatic activities increased significantly with respect to unrestored soils. Similarly, a positive priming effect of soil organic matter mineralisation was produced by all organic amendments, being significantly higher (p < 0.05) in sewage sludge-treated soils. Bacterial diversity was higher in restored than in control soils. The restoration caused changes in soil bacterial communities' composition at the phylum and genus levels. It was observed that soil bacterial communities were significantly related to several physical, chemical and biochemical soil properties, establishing two different co-occurrence patterns between restored and unrestored soils. A first bacterial co-occurrence pattern showed significant positive correlations to pH and C/N ratio and negativity with the rest of the soil properties. The second bacterial pattern was positively correlated with carbohydrates, μg of C, priming effect, BR, β-glucosidase and phosphatase and negatively with pH and C/N ratio. It was concluded that soil bacterial communities are clearly influenced by the types of organic amendments applied. Bacterial taxa such as Taibaiella or Pseudomonas could perform key functions in the carbon cycle in restored soils.
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•Recycled waste improved physico-chemical and biological quality in restored soils.•Organic amendment modifies soil bacterial communities composition in the short-term.•Two soil bacterial co-occurrence pattern were found in restored and degraded soils.•Specific soil bacterial taxa could perform key functions in the carbon cycle.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
EBV+ diffuse large B cell lymphoma (DLBCL) not otherwise specified (NOS) is a new entity confirmed by the World Health Organization (WHO) in 2017. In this new entity, the virus may contribute to a ...tolerogenic microenvironment. Traces of the virus have been described in DLBCL with more sensitive methods, in cases that were originally diagnosed as negative. The aim of this study was to analyze the expression of immune response genes in the tumor microenvironment to disclose the role of the virus and its traces in DLBCL. In 48 DLBCL cases, the expression of immune response genes and the presence of molecules that induce tolerance, such as
TIM3
,
LAG3
and
PDL1
by immunohistochemistry (IHC), were studied. To broaden the study of the microenvironment, tumor-associated macrophages (TMAs) were also explored. No significant differences were observed in the expression of immune response genes in the EBV+ DLBCL and those cases that were EBV− DLBCL but that exhibited viral traces, assessed by ViewRNA assay. Only the EBV+ DLBCL cases displayed a significantly higher increase in the expression of
CD8
and cytotoxic T cells detected by gene expression analysis, and of PDL1 in tumor cells and in the expression of CD68 in the tumor microenvironment detected by IHC, not observed in those cases with viral traces. The increase in CD8 and cytotoxic T cells, PDL1 and CD68 markers only in EBV+ DLBCL may indicate that traces of viral infection might not have influence in immune response markers.
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EMUNI, NUK, SBMB, SBNM, UL, UM, UPUK
The synthesis of glycerol carbonate from glycerol and dimethyl carbonate by transesterification is reported. Basic catalysts work much better than acidic ones. Catalytic activity is strongly ...dependent on base strength. After a catalyst screening study, CaO was selected as catalyst. Following an optimization study a ∼100% conversion and a 95% yield were achieved in 90
min at 95
°C, 0.06 catalyst:glycerol molar ratio and 3.5 dimethyl carbonate:glycerol molar ratio.
The synthesis of glycerol carbonate from glycerol and dimethyl carbonate by transesterification is reported. Firstly, a catalyst screening has been performed by studying the influence of different basic and acid homogeneous and heterogeneous catalysts on reaction results. Catalytic activity is extremely low for acidic catalysts indicating that reaction rate is very slow. On the contrary, high conversions and yields are obtained for basic catalysts. Catalytic activity increases with catalyst basic strength. The best heterogeneous catalyst is CaO. Calcination of CaO increases dramatically its activity due to calcium hydroxide removal from its surface. A reaction optimization study has been carried out with CaO as catalyst by using a factorial design of experiments leading to operation conditions for achieving a 100% conversion and a >95% yield at 1.5
h reaction time: 95
°C, catalyst/glycerol molar ratio
=
0.06 and dimethyl carbonate/glycerol molar ratio
=
3.5. Carbonate glycerol can be easily isolated by filtering the catalyst out and evaporating the filtrate at vacuum. Leaching of catalyst in reaction medium was lower than 0.34%. Catalyst recycling leads to a quick decrease in both conversions and yields probably due to a combination of catalyst deactivation by CaO exposure to air between catalytic runs, and a decrease in the catalyst surface area available for reaction due to particle agglomeration.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
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