By removing the immunogenic cellular components through various decellularization methods, decellularized extracellular matrix (dECM) is considered a promising material in the field of tissue ...engineering and regenerative medicine with highly preserved physicochemical properties and superior biocompatibility. However, decellularization treatment can lead to some loss of structural integrity, mechanical strength, degradation stability, and biological performance of dECM biomaterials. Therefore, physical and chemical crosslinking methods are preferred to restore or even improve the biomechanical properties, stability, and bioactivity, and to achieve a delicate balance between degradation of the implanted biomaterial and regeneration of the host tissue. This review provides an overview of dECM biomaterials, and describes and compares the mechanisms and characteristics of commonly used crosslinking methods for dECM, with a focus on the potential applications of versatile dECM‐based biomaterials derived from skin, cardiac tissues (pericardium, heart valves, myocardial tissue), blood vessels, liver, and kidney, modified with different chemical crosslinking reagents, in tissue and organ regeneration.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
The development of bifunctional electrochemically-active micro-/nanomaterials with heterostructures for both supercapacitors and hydrogen evolution reaction (HER) enables the possibility to integrate ...energy storage and conversion into one single system. Herein, heterostructural NiCo2S4@MoS2 materials have been successfully prepared using zeolitic imidazolate frameworks as templates, including transformation to bimetallic layered double hydroxide, and sequential sulfuration. The smart process entails an assembly of MoS2 nanosheets on NiCo2S4 nanobuilding blocks with hollow core–shell nanoarchitectures. Specifically, when tested as an electrode in a supercapacitor, it can deliver an increased specific capacitance of 860 F g−1 at a current density of 1 A g−1, superior rate capability and cyclic stability. Furthermore, the NiCo2S4@MoS2 heterostructure also efficiently electrocatalyzes the HER in an alkaline electrolyte with a low overpotential of 194 mV, a favorable HER kinetics and better long-term stability. Results of both scenarios attest to the enhanced electrochemical performance of the NiCo2S4@MoS2 heterostructure compared to its NiCo2S4 counterpart, which is attributed to the hollow porous core–shell structures, nanointerface engineering and the synergistic effect between NiCo2S4 and MoS2. This work will broaden our horizons in designing and fabricating multifunctional active materials for next generation energy storage and conversion applications.
We report the plasmonic enhancement of the photocatalytic properties of Pt/n-Si/Ag photodiode photocatalysts using Au/Ag core/shell nanorods. We show that Au/Ag core/shell nanorods can be synthesized ...with tunable plasmon resonance frequencies and then conjugated onto Pt/n-Si/Ag photodiodes using well-defined chemistry. Photocatalytic studies showed that the conjugation with Au/Ag core/shell nanorods can significantly enhance the photocatalytic activity by more than a factor of 3. Spectral dependence studies further revealed that the photocatalytic enhancement is strongly correlated with the plasmonic absorption spectra of the Au/Ag core/shell nanorods, unambiguously demonstrating the plasmonic enhancement effect.
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IJS, KILJ, NUK, PNG, UL, UM
Coastal groundwater is an important resource in the developed region associated with human health and sustainable economic development. To identify the origins of salinity and evaluate the impact of ...water-rock interactions, seawater intrusion (SWI), and evaporation on groundwater in the coastal areas of Zhejiang and Fujian provinces, a comprehensive investigation was performed. Meanwhile, nitrate and fluoride indicators resulting from the anthropogenic activity and SWI were also considered. At last, the water quality index (WQI) of coastal groundwater was evaluated with geochemical and multivariate statistical methods. The results indicated that (1) the groundwater in coastal areas of Zhejiang and Fujian provinces has been affected by SWI to varying degrees. The analysis of selected ion ratios (Na+/Cl- and Br-/Cl-) and isotopic compositions showed that SWI is the predominant cause of increasing salinity in the groundwater of Zhejiang Province, while the cause is water-rock interactions (ion exchange and mineral weathering) in Fujian Province. The hydrochemical evolution path of groundwater in Zhejiang Province is Ca/Mg-HCO3 to Na-Cl, while a different pattern of Ca/Mg-HCO3 to Na (Mg/Ca)-Cl occurs in Fujian Province. However, the trend of SWI development in both provinces was freshening. (2) Nitrification, sewage infiltration, and SWI increased the NO3- content in groundwater. Some of the NO3- concentration in Fujian Province exceeds the standard, and the nitrogen pollution was more serious than in Zhejiang Province. The F- content in coastal groundwater was affected by SWI and mineral dissolution; the F- content in Zhejiang Province was higher than in Fujian Province, which was close to the groundwater standard limit. The average WQI value of Zhejiang was 103.61, and the WQI of Fujian was 61.69, indicating that the coastal groundwater quality in Fujian Province was better than in Zhejiang Province. The results of the study revealed the impact of SWI and anthropogenic activity on groundwater in the southern coastal zone of China and will be valuable for sustainable groundwater resource management.
Oxaliplatin is the first-line regime for advanced gastric cancer treatment, while its resistance is a major problem that leads to the failure of clinical treatments. Tumor cell heterogeneity has been ...considered as one of the main causes for drug resistance in cancer. In this study, the mechanism of oxaliplatin resistance was investigated through in vitro human gastric cancer organoids and gastric cancer oxaliplatin-resistant cell lines and in vivo subcutaneous tumorigenicity experiments. The in vitro and in vivo results indicated that CD133+ stem cell-like cells are the main subpopulation and PARP1 is the central gene mediating oxaliplatin resistance in gastric cancer. It was found that PARP1 can effectively repair DNA damage caused by oxaliplatin by means of mediating the opening of base excision repair pathway, leading to the occurrence of drug resistance. The CD133+ stem cells also exhibited upregulated expression of N6-methyladenosine (m6A) mRNA and its writer METTL3 as showed by immunoprecipitation followed by sequencing and transcriptome analysis. METTTL3 enhances the stability of PARP1 by recruiting YTHDF1 to target the 3′-untranslated Region (3′-UTR) of PARP1 mRNA. The CD133+ tumor stem cells can regulate the stability and expression of m6A to PARP1 through METTL3, and thus exerting the PARP1-mediated DNA damage repair ability. Therefore, our study demonstrated that m6A Methyltransferase METTL3 facilitates oxaliplatin resistance in CD133+ gastric cancer stem cells by Promoting PARP1 mRNA stability which increases base excision repair pathway activity.
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EMUNI, FZAB, GEOZS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NUK, OILJ, PNG, SAZU, SBCE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Plant architecture is determined by genetic and developmental programs as well as by environmental factors. Sessile plants have evolved a subtle adaptive mechanism that allows them to alter their ...growth and development during periods of stress. Phytohormones play a central role in this process; however, the molecules responsible for integrating growth- and stress-related signals are unknown. Here, we report a gain-of-function rice (Oryza sativa) mutant, tld1-D, characterized by (and named for) an increased number of tillers, enlarged leaf angles, and dwarfism. TLD1 is a rice GH3.13 gene that encodes indole-3-acetic acid (IAA)-amido synthetase, which is suppressed in aboveground tissues under normal conditions but which is dramatically induced by drought stress. The activation of TLD1 reduced the IAA maxima at the lamina joint, shoot base, and nodes, resulting in subsequent alterations in plant architecture and tissue patterning but enhancing drought tolerance. Accordingly, the decreased level of free IAA in tld1-D due to the conjugation of IAA with amino acids greatly facilitated the accumulation of late-embryogenesis abundant mRNA compared with the wild type. The direct regulation of such drought-inducible genes by changes in the concentration of IAA provides a model for changes in plant architecture via the process of drought adaptation, which occurs frequently in nature.
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BFBNIB, NMLJ, NUK, PNG, SAZU, UL, UM, UPUK
Two luminescent Cd(ii)–organic frameworks Cd2(L1)(tdc)2(H2O)n (1) and Cd(L2)0.5(tdc)n (2) (L1 = 1,5-bis(1-(pyridine-4-ylmethyl)-1H-benzodimidazol-2-yl)pentane, L2 = ...1,6-bis(1-(pyridine-4-ylmethyl)-1H-benzodimidazol-2-yl)hexane, and H2tdc = 2,5-thiophenedicarboxylic acid) were hydrothermally synthesized and characterized. 1 displays a rare binodal (3,4)-connected 2D cem-d network, while 2 exhibits a 3D mog (moganite) framework. The two MOFs are highly thermally durable and water-stable in a wide pH range from 3 to 12. Interestingly, 1 and 2 represent the first reported examples of multi-responsive probes based on MOFs for selectively detecting levofloxacin, benzaldehyde, and Fe3+ ions with reusability. The luminescence sensing mechanisms of the two CPs were explored in detail.
CDK4/6 inhibitors show promising antitumor activity in a variety of solid tumors; however, their role in head and neck squamous cell carcinoma (HNSCC) requires further investigation. The ...senescence-associated secretory phenotype (SASP) induced by CDK4/6 inhibitors has dual effects on cancer treatment. The need to address the SASP is a serious challenge in the clinical application of CDK4/6 inhibitors. We investigated whether metformin can act as a senostatic drug to modulate the SASP and enhance the anticancer efficacy of CDK4/6 inhibitors in HNSCC. In this study, the efficacy of a combination of the CDK4/6 inhibitor LY2835219 and metformin in HNSCC was investigated in in vitro assays, an HSC6 xenograft model, and a patient-derived xenograft model. Senescence-associated β-galactosidase staining, antibody array, sphere-forming assay, and in vivo tumorigenesis assay were used to detect the impacts of metformin on the senescence and SASP induced by LY2835219. We found that LY2835219 combined with metformin synergistically inhibited HNSCC by inducing cell cycle arrest in vitro and in vivo. Metformin significantly modulated the profiles of the SASP elicited by LY2835219 by inhibiting the mTOR and stat3 pathways. The LY2835219-induced SASP resulted in upregulation of cancer stemness, while this phenomenon can be attenuated when combined with metformin. Furthermore, results showed that the stemness inhibition by metformin was associated with blockade of the IL6-stat3 axis. Survival analysis demonstrated that overexpression of IL6 and stemness markers was associated with poor survival in HNSCC patients, indicating that including metformin to target these proteins might improve patient prognosis. Collectively, our data suggest that metformin can act as a senostatic drug to enhance the anticancer efficacy of CDK4/6 inhibitors by reprogramming the profiles of the SASP.
In general, vibration signals generated by the switching operation of a high-voltage circuit breaker (HVCB) contains important information to reflect its mechanical status. A method for mechanical ...fault diagnoses of an HVCB based on a semisupervised stacked autoencoder (SSAE) and an integrated extreme learning machine (IELM) is proposed in this study. First, the complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN) is used to decompose the vibration signal to obtain the time-frequency energy matrix. Then, an SSAE model is applied to automatically extract the characteristic information from the energy matrix. As a result, two-level classifiers can be constructed. The first level is utilized to identify normal or abnormal states, and the second level is selected to identify various types of faults in the abnormal state. The classifiers of these levels are composed of binary IELM. The advantages of the proposed method are that it not only can automatically extract the high-recognition features from the time-frequency energy matrix of high dimension to complete the identification of the existing fault types in the training set but also can accurately identify the samples of unknown types of faults. Experimental results show that the proposed method can effectively diagnose mechanical faults of an HVCB, and the classification accuracy reaches 99.5%.
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