In loess the aggregate is the basic structural unit, and its stability is an important factor affecting the composition, water stability and strength of loess. However, due to the difficulty of ...sample preparation, few scholars have done independent research on it. In this manuscript, a numerical model of aggregate is constructed by the discrete element method. Under the continuous action of certain stress, the uninterrupted development process of sample deformation with time was observed, that is, the creep of aggregate structures. The results show that the creep of aggregates is closely related to the relative movement, rotation and rearrangement of internal structural elements, and the most intuitive mesoscopic evolution of the adjustment process of structural elements is the change of contact number, namely the coordination number. The microscopic parameters and evolutionary characteristics of fabric can reveal the microscopic mechanism behind the macroscopic creep phenomenon. With the creep process, the creep stress is gradually borne by the normal contact force rather than the tangential contact force and has anisotropic characteristics. As a result of creep, the contact points of particles increase, and the interaction between aggregates changes from point contact to overlap contact. The constraint between aggregates increases, and the skeleton tends to be a more stable structure, which can bear a larger load.
An impedance matched parametric amplifier (IMPA) with Josephson junctions is fabricated and characterized. A hybrid structure containing coplanar and strip structures is implemented to realize an ...impedance taper line and a plate capacitor in an LC nonlinear resonator based on Josephson junctions. The upper plate of the capacitor is isolated with SiN
x
without grounding as well as the strips. Such easily-prepared designs greatly reduce the requirements for lithography alignment and precision, which makes the fabrication process more reliable. The experimental results show that in such IMPA a gain higher than 25 dB with a bandwidth of about 100 MHz can be obtained. This broadband amplifier operates close to the quantum limit. By adjusting the working point, a higher bandwidth of about 400 MHz can be obtained with a gain of about 17 dB.
Glycogen synthase kinase-3β (GSK-3β) is a serine/threonine kinase involved in cancer development. Herein, we demonstrated the role of GSK-3β in endometrial cancer (EC) and identified new therapeutic ...targets.
GSK-3β was overexpressed in EC tissues, and was positively correlated with International Federation of Gynecology and Obstetrics (FIGO) staging, dedifferentiation, and myometrial infiltration depth. Besides, GSK-3β overexpression predicted lower cumulative and relapse-free survival rate. si-GSK-3β transfection suppressed cell proliferation, migration, invasion, and promoted cell apoptosis through downregulating NF-kB, Cyclin D1 and MMP9 expression whereas upregulating P21 expression. Bioinformatic predictions and dual-luciferase reporter assays showed that GSK-3β was a possible target of miR-129. MiR-129 transfection reduced GSK-3β expression, and exhibited the same trend as si-GSK-3β transfection in cell function experiments. The nude mouse xenograft assay showed that miR-129 overexpression may suppress tumor growth through downregulating GSK-3β expression. Further studies showed that AZD1080, a GSK-3β inhibitor, could also inhibit EC cell proliferation, migration and invasion, while induced cell apoptosis through modulating relevant genes downstream of GSK-3β signaling.
GSK-3β expression was determined in EC tissue and normal endometrial tissues by immunohistochemistry. After GSK-3β down-regulation by si-GSK-3β, microRNA-129 mimic transfection or GSK-3β inhibitor exposure, EC cell phenotypes and related molecules were examined.
Our results demonstrate for the first time that GSK-3β may be a novel and important therapeutic target for the treatment of endometrial carcinoma. GSK-3β inhibitor AZD1080 may be an effective drug for treating endometrial carcinoma.
Colorectal cancer(CRC) is a prevalent malignancy in the world. There is growing evidence that microRNAs (miRNAs) as crucial modulator are in connection with many tumor-related diseases including CRC. ...Though miR-485-5p has been reported as an anti-oncogene in certain cancers, it remains unclear in CRC. In this research, we found that miR-485-5p was at lower level expression in CRC tissues and cell lines compared to the paired paracancerous tissues and the normal colon epithelial cell line FHC, correspondingly. Furthermore, Experimental up-regulation miR-485-5p in DLD-1 and SW480 cells with mimic could inhibit the ability of proliferation, migration, invasion of CRC cell lines and facilitate cells apoptosis. Also, we confirmed that CD147 existed typically negative regulation by miR-485-5p through binding a conserved sequence specifically within the CD147 3'-untranslated region (3'UTR) and reintroduction of CD147 could rescue the phenotypic changes caused by miR-485-5p. The findings provide evidence to demonstrate the role of miR-485-5p/CD147 interaction in CRC and indicate that miR-485-5p might be exploited therapeutically in CRC.
Mechanical forces are indispensable for bone healing, disruption of which is recognized as a contributing cause to nonunion or delayed union. However, the underlying mechanism of mechanical ...regulation of fracture healing is elusive.
We used the lineage-tracing mouse model, conditional knockout depletion mouse model, hindlimb unloading model and single-cell RNA sequencing to analyze the crucial roles of mechanosensitive protein polycystin-1 (PC1,
) promotes periosteal stem/progenitor cells (PSPCs) osteochondral differentiation in fracture healing.
Our results showed that cathepsin (
)-positive PSPCs are fracture-responsive and mechanosensitive and can differentiate into osteoblasts and chondrocytes during fracture repair. We found that polycystin-1 declines markedly in PSPCs with mechanical unloading while increasing in response to mechanical stimulus. Mice with conditional depletion of
in
PSPCs show impaired osteochondrogenesis, reduced cortical bone formation, delayed fracture healing, and diminished responsiveness to mechanical unloading. Mechanistically, PC1 facilitates nuclear translocation of transcriptional coactivator TAZ via PC1 C-terminal tail cleavage, enhancing osteochondral differentiation potential of PSPCs. Pharmacological intervention of the PC1-TAZ axis and promotion of TAZ nuclear translocation using Zinc01442821 enhances fracture healing and alleviates delayed union or nonunion induced by mechanical unloading.
Our study reveals that
PSPCs within the callus can sense mechanical forces through the PC1-TAZ axis, targeting which represents great therapeutic potential for delayed fracture union or nonunion.
Aim: To compare the pregnancy outcomes of frozen-thawed embryo transfer (FET) cycles among women with repeated implantation failure (RIF) treated with various endometrial preparation protocols. ...Methods: A total of 605 women with RIF were retrospectively recruited between January 2017 and December 2020 from Northern Theater General Hospital. Patients were divided into natural cycles, hormone replacement therapy (HRT) cycles, depot gonadotropinreleasing hormone (GnRH) agonist-HRT, and endometrial scratching (ES) plus depot GnRH agonist-HRT. The primary endpoint was clinical pregnancy rate, while secondary endpoints included live birth rate and pain assessment. Results: Of the 605 recruited patients, 63 were undergoing natural cycles, 281 were treated with HRT cycles, 141 treated with depot GnRH agonist-HRT, and 120 treated with ES combined with depot GnRH agonist-HRT. There were significant differences among protocols on clinical pregnancy rate (P=0.029), while no significant difference was observed among protocols on live birth rates (P=0.108). Multivariate analyses suggested that HRT (odds ratio OR: 0.50; 95% confidence interval CI: 0.28-0.89; P=0.019) and depot GnRH agonist-HRT (OR: 0.49; 95% CI: 0.27-0.91; P=0.021) cycles were associated with a lower clinical pregnancy rate as compared with natural cycles, while no significant difference between ES combined with depot GnRH agonist-HRT and natural cycles for clinical pregnancy rates (OR: 0.72; 95% CI: 0.38-1.36; P=0.313). Moreover, the HRT (OR: 0.70; 95% CI: 0.39-1.28; P=0.239), depot GnRH agonist-HRT (OR: 0.67; 95% CI: 0.35-1.29; P=0.229), and ES combined with depot GnRH agonist-HRT (OR: 1.11; 95% CI: 0.58-2.14; P=0.754) cycles had no significant effects on live birth rate as compared with natural cycles. A total of 87.50% patients treated with ES combined with depot GnRH agonist-HRT reported pain during the procedure. Conclusion: ES and depot GnRH agonists could be considered for RIF women with high-quality blastocysts, 14 days after verified transplantation failure. Keywords: endometrial scratching, depot GnRH agonist, repeated implantation failure, blastocysts, clinical pregnancy rate, live birth rate
Highly integrated aerospace products have a small interior space; thus, heat pipes (HPs) with a small bending radius are required for thermal management. An HP sample with an ultra-small bending ...radius and a capillary channel is fabricated by 3D printing. The cross-sectional area of the sample and its heat transfer capacity are compared with that of a standard aluminum-ammonia channel heat pipe (ACHP). The L-shaped HP samples consist of three sections: two straight parts (L1 and L2) and a curved part with a bending radius of R. A prototype is designed and manufactured for an on-orbit flight test. Statistical methods are used to calculate the filling volume of the working fluid. The maximum heat flow of the HP sample A is 140.0 W at a cold plate temperature of 10.0 ℃, and the thermal resistance is 0.01 ~ 0.07 K/W. The maximum heat-transport capacity of HP sample A is 66.3 ~ 88.4 W•m at different condensation temperatures, which is 4.0% ~ 18.4% lower than that of HP samples B and C. The on-orbit flight data show that the temperature difference between the two ends of the 3D printed HP is 0.001 ~ 0.997 K, and the working condition and performance are excellent.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
The vegetable greenhouse soils in Yanglou Town, Ruzhou City, Henan Province were taken as the research object in the present study to explore the difference in soil physical and chemical properties ...and the total and fraction of heavy metals of different planting years. The potential ecological risks of heavy metals in greenhouse soils with different planting years were assessed by using single and comprehensive potential ecological risk index methods. The results showed that the soil pH of vegetable greenhouses increased, and fertility factors such as organic matter, available phosphorus, and alkali-hydrolyzable nitrogen accumulated to a certain extent compared to the control group, whereas catalase showed a decreasing trend. Correlation analysis showed that the planting years were significant positively correlated with pH (
<0.05) and organic matter (
<0.01) and significant negatively correlated with catalase (
<0.01). The amount of heavy metals in the vegetable greenhouse soils increased with the increase i
Dicyandiamide（DICY） is a common ligand that exhibits low toxicity but can irritate the skin and eyes and cause methemoglobinemia on long-term exposure. Crystalline Cu-dicyandiamide（Cu-DICY） was ...obtained via facile synthesis and its molecular structure and theoretical Raman spectra were simulated by using density functional theory（DFT）. The results suggested that the Cu~（2＋） coordinates with two H_2 O molecules and two different DICY molecules（an imino DICY and an amino DICY）. The stability constants of Cu-DICY were calculated, and the electrochemical properties were studied. Two electrochemical redox processes occur in Cu-DICY in an aqueous solution： a reversible reaction with a formal potential of 0.2 V vs.MSE and an irreversible reaction between –0.4 and –1.2 V vs. MSE. The standard rate constant k0 for the reversible reaction was estimated to be 7.6×10~（-3） cm/s. In addition, based on the reversible reaction of Cu-DICY, square wave voltammetry was used to rapidly determine the concentration of Cu（II） and the detection limit was 66.7 μg/L, which satisfies the detection limit requirements for copper in tap water（2 mg/L） as provided by the World Health Organization.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
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