BackgroundAnisakiasis-related gastric eosinophilic granuloma is rare. Case ReportHerein, we report a patient with anisakiasis-related gastric eosinophilic granuloma who was treated with laparoscopic ...and endoscopic cooperative surgery (LECS). A 59-year-old woman was presented to our hospital for further examination of a gastric lesion that was initially diagnosed by a local medical doctor. Esophagogastroduodenoscopy showed a submucosal tumor-like lesion in the lower body of the stomach. Endoscopic ultrasonography showed a heterogeneous hypoechoic submucosal mass lesion in the submucosal layer measuring 10 mm, without evidence of deep involvement. Under a clinical diagnosis of gastrointestinal stromal tumor, the patient underwent LECS. Gross appearance of the resected specimen revealed a 1.5×1.0 cm submucosal tumor-like lesion. Microscopic examination revealed necrosed insects consistent with the characteristics of gastric anisakiasis, around which prominent eosinophilic infiltration and granulomas were observed. This prompted a diagnosis of gastric eosinophilic granuloma related to anisakiasis. ConclusionTo the best of our knowledge, this is the second case of gastric eosinophilic granuloma related to anisakiasis resected by LECS in the English medical literature. LECS might be a useful procedure for minimally invasive therapeutic diagnosis.
Urease was immobilized in a porous membrane by coating the surfaces of both sides with plasma-polymerized 2-propyn-1-ol (propargyl alcohol), and the membrane was attached to an ammonia electrode to ...compose a urea sensor. The sensor was quite sensitive when cellulose having random pore sizes which averaged 2200Å in diameter was employed as a substrate membrane. However, the membrane made from polycarbonate and having straight pores with a regular diameter of 150Å was chosen because of good reproducibility in terms of electrode responses. Standard deviations of the electrode responses with the proposed sensor were approximately 1 mV in the steady-state method and nearly 0.5 mV/min in the reaction rate method. Ten consecutive measurements little changed the reproducibility. The sensor worked more than 2 months. During the time, the magnitude of the response gradually increased, and the time to reach the maximum response shortened regardless of the methods for measurement. The immobilized urease membrane effectively protected from p-benzoquinone that was known as an inhibitor against the enzyme reaction due to its electrophilic nature. The property of the monomer for the plasma polymerization affected optimum pH for the electrode response.
Immobilized glucose oxidase (GOD) and urease membranes were prepared by impregnating porous substrates with respective enzymes and coating of plasma-polymerized propargyl alcohol (PPPA) on both ...surfaces of the porous substrates. The GOD membrane was attached to an oxygen electrode to work as a glucose sensor and the urease membrane was assembled with an ammonia electrode to be an urea sensor. Working curves of the two electrodes showed different behaviors depending upon the amount of enzymes immobilized, porous substrates chosen, and pH of the sample solutions. In the case of glucose sensor, a small disk of Millipore-VS membrane was employed as a substrate to impregnate with 0.76 or 3.78 U/cm2 of GOD that was subsequently immobilized by the plasma-coating. The sample solutions were regulated at pH 7 using a phosphate buffer solution. The urease membrane was prepared by impregnating Nuclepore-PC with 0.0320.039 U/cm2 of urease and plasma-coated as above. Although the enzyme is most active at pH 67, the sample solution was better controlled at pH 8 to facilitate the transportation of ammonia towards the ammonia electrode.
Recently, intracellular yeast-like symbionts were isolated from the eggs of the brown planthopper, Nilaparavata lugens . The authors attempted to isolate the antimicrobial substances produced by ...these intracellular symbionts to elucidate the symbiotic relationship between the host insect and the microorganisms. In this paper, the authors describe the taxonomical results for a selected strain from the symbionts and the identification of the active substance produced by it. From the properties described above and the physiological characteristics, the strain was determined to belong to the genus Bacillus .
Wound healing is very imortant in surgery. In the process of wound healing, the development of granulation tissue is necessary, and here the enhancement of insoluble fibrin is essential. As ...development of the insoluble fibrin clot plays an important role in the process of wound healing, we carried out studies to immobilize thrombin and Factor XIII on absorbable gelatin sponge and suture materials, which would hopefully enhance the local accumulation of fibrin. A gelatin sponge sheet (5×2.5×0.5cm) was immersed into a solution containing thrombin and Factor XIII and then it was lyophilized. DEXON (polyglycolic acid gut) and Cat gut were also treated by the same methods. Nylon gut had been dehydrolized with 3N HCl and then thrombin and Factor XIII were immobilized on the surface. Each material was incubated in normal human plasma to test the activities of the proteins immobilized. Fibrinogen levels and FDP in the plasma were measured before and after the incubation. Fibrinogen levels were detected by the method of turbidimetric analysis and FDP, by Latex coagulation method. All materials were studied histologically. Non-treated materials were served as controls. The fibrinogen levels in the plasma incubated with the immobilized materials decreased remarkably and the FDP levels varied. The histology showed that a large amount of fibrin had accumulated on the treated materials, however, no fibrin formation was observed on the controls. In vivo study is now underway.
Purified mumps virus (Urabe strain) was given mainly by intravenous injection to a total of 200 patients with cancer. The only adverse clinical reaction was transient mild fever in about half the ...patients. The beneficial clinical effects were as follows; decrease or disappearance of ascites and edema of the lower limbs at high rates (26/37 and 4/4, respectively), usually within a week after treatment: decrease or stoppage of cancerous bleeding in 30 of 35 patients: decrease or disappearance of pain in most of the patients: and tumor regression in 26 patients with cancer of the breast, rectum, ileocaecum, thyroid gland, uterus, skin, etc. Histologically, the virus-treatment caused shrinkage of nuclei and vacuolization of the cytoplasma of tumor cells, but the degenerative changes of tumor cells were not so great as those after chemotherapy or radiotherapy. Infiltration of lymphocytes, fibrosis and collagenesis occurred around tumor tissues, where necrosis or exfoliation of tumor cells was frequently observed.
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