Um dieser Herausforderung entgegentreten zu können, wurde „Drink Smart“, eine Active & Assisted Living- Lösung, entwickelt. Das zweite Ziel — ebenfalls unter Performanz fallend — war die ...Kommunikation des Drink Smart Systems mit einer elektronischen Pflegedokumentation bzw. das Angebot einer Stand-Alone-Lösung mithilfe einer Smartphone Applikation. Für die Evaluation der Projektziele wurden den Überkategorien Forschungsfragen zugeordnet, beispielsweise _ Welche technische Performanz erreicht das Drink Smart System im Alltag und in der Nutzung durch die Zielgruppen? _ Welche Funktionalitäten konnten bzw. konnten nicht umgesetzt werden? _ Welche technische Performanz erreicht das System in einer praxisnahen Anwendung? _ Welche Probleme können in der Gebrauchstauglichkeit während des Pre-Trails identifiziert werden? _ Welche Usability-Probleme bzw. Während der Pre-Trials fand eine heuristische Evaluation durch eine Expertin in Bezug auf die Usability sowie eine Testung auf Stabilität, Funktionalität und Einsatzsicherhit durch Projektmitarbeiter der Fachhochschule Campus Wien (FHCW) und der MIK — mobile, individuelle Krankenpflege -OG statt.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Nanoparticles that are aimed at targeting cancer cells, but sparing healthy tissue provide an attractive platform of implementation for hyperthermia or as carriers of chemotherapeutics. According to ...the literature, diverse effects of nanoparticles relating to mammalian reproductive tissue are described. To address the impact of nanoparticles on cyto- and genotoxicity concerning the reproductive system, we examined the effect of superparamagnetic iron oxide nanoparticles (SPIONs) on granulosa cells, which are very important for ovarian function and female fertility. Human granulosa cells (HLG-5) were treated with SPIONs, either coated with lauric acid (SEONLA) only, or additionally with a protein corona of bovine serum albumin (BSA; SEON(LA-BSA)), or with dextran (SEON(DEX)). Both micronuclei testing and the detection of γH2A.X revealed no genotoxic effects of SEON(LA-BSA), SEON(DEX) or SEON(LA). Thus, it was demonstrated that different coatings of SPIONs improve biocompatibility, especially in terms of genotoxicity towards cells of the reproductive system.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Since superparamagnetic iron oxide nanoparticles (SPION) possess unique features, they provide a huge platform for medical applications, especially for cancer diagnosis and therapy (e.g. imaging, and ...drug targeting). However, heterogeneous effects on mammalian cells with regard to reproductive tissue are described. An experimental study was carried out to study the effects of SPIONs on both the expression of steroid hormone receptor and viability of granulosa cells, which play a key role in ovarian health and fertility.
Human granulosa cells were cultured in vitro and incubated with different concentrations of SPIONs. After 48 h, steroid receptor expression and cell viability were evaluated.
Treatment of granulosa cells with SPIONs did not affect estrogen receptor β1 or progesterone receptor-A expression and had no significant effect on cell viability.
Nanoparticles precoated with bovine serum albumin (BSA) do not alter granulosa cell phenotype, whereas literature suggests that other nanoparticles induce apoptosis and reduce steroid receptor expression. Our data indicate an overall better outcome using SPIONs coated with BSA.
Nanoparticles that are aimed at targeting cancer cells, but sparing healthy tissue provide an attractive platform of implementation for hyperthermia or as carriers of chemotherapeutics. According to ...the literature, diverse effects of nanoparticles relating to mammalian reproductive tissue are described. To address the impact of nanoparticles on cyto- and genotoxicity concerning the reproductive system, we examined the effect of superparamagnetic iron oxide nanoparticles (SPIONs) on granulosa cells, which are very important for ovarian function and female fertility. Human granulosa cells (HLG-5) were treated with SPIONs, either coated with lauric acid (SEONLA) only, or additionally with a protein corona of bovine serum albumin (BSA;SEONLA-BSA),or with dextran (SEONDEX). Both micronuclei testing and the detection of H2A.X revealed no genotoxic effects of SEONLA-BSA, SEONDEX or SEONLA. Thus, it was demonstrated that different coatings of SPIONs improve biocompatibility, especially in terms of genotoxicity towards cells of the reproductive system.
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