The calculation of accurate collision strengths for atomic transitions has been a long standing problem in quantitative spectroscopy. Most modern calculations are based on the R-matrix method and ...problems pertaining to the use of this method have led to a discussion of the accuracy of these results. More in particular, based on an analysis of the spectra of NGC 3918 and NGC 6302, Clegg et al. (1987) and Oliva et al. (1996) have questioned R-matrix calculations for the infrared Ne V fine-structure transitions. Using improved flux measurements for the Ne V lines, we show that the conclusion that these collision strengths would be too high, is not correct. The discrepancies found by Clegg et al. (1987) can be explained by the inaccuracy of the Ne V 342.6 nm flux they adopted. The discrepancies found by Oliva et al. (1996) can be explained by the inaccuracy of the LRS flux for the Ne V 14.32 micron line. Based on the data presented in this paper there is no reason to assume that there are any problems with the R-matrix calculations for Ne^4+ of Lennon & Burke (1994). We show that the data are accurate at the 30 % level or better. This confirms the validity of the close coupling method.
A mounting body of evidence suggests that cytoplasmically synthesized proteins destined to be imported into the mitochondrial interior must at least partly unfold to penetrate across the ...mitochondrial membranes. During post-translational import, this unfolding process appears to be a major rate-limiting step. It can be blocked by ligands that stabilize the protein’s native conformation and appears to be accompanied by the cleavage of ATP outside the mitochondrial inner membrane.
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A quantitative analysis of the proliferative response induced in murine thymocytes by concanavalin A (Con A) is described. Exogenous 3H-thymidine labels 35 to 40% of the newly incorporated TMP ...residues under optimal conditions. The density label 5-bromo-2-deoxuridine (BrUdR) does not affect DNA metabolism in this system. With this nucleoside, it is shown that newly synthesized DNA is the result of semi-conservative replication, not repair. Double labeling of DNA provides a monitor for cells traversing the cell cycle (S phase to subsequent S phase). The average cycle time is 12.5 hr, and the shortest cell cycle time is 10 hr. The growing fraction of active cells is about two-thirds. The data show that different subpopulations of thymocytes begin proliferating after various times in culture. Once effectively stimulated by Con A, some of the cells can traverse the cell cycle at least twice more after the mitogen is removed.
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