Based on the triple bottom line of sustainable development, sustainable entrepreneurship has been well studied; however, the existing literature on the measurement of sustainable entrepreneurship is ...still limited. This paper attempts to quantify sustainable entrepreneurship through three indicators, innovative spirit, entrepreneurial spirit and decision-making spirit, and constructs a new time-varying index of sustainable entrepreneurship by the time-varying coefficient state space equation model. Applying the new time-varying index, this paper studies the impact of sustainable entrepreneurship on Chinese regional economic growth and explores the role of technical R&D and financial intermediary development in the path of influence. The empirical results show following conclusions. First, sustainable entrepreneurship promotes regional economic growth. Second, there exists a positive mediation effect of sustainable entrepreneurship influencing regional economic growth through technical R&D. Third, financial intermediaries have a positive effect on sustainable entrepreneurship, and they play a moderating role in the first half of the path through which sustainable entrepreneurship affects regional economic growth through technical R&D.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
This study is a quantitative study based on the questionnaire data provided by cfps, which mainly studies the influence of parents’ intervention in students’ online time and online content on their ...children’s school performance. In the end, this study concluded that parental intervention and control can indeed have a positive impact on children’s school performance to some extent.
With the surge of digital tide, the digital use of intangible cultural heritage is increasingly common. In recent years, scholars have focused on digital technology as an important force in enabling ...the development of intangible cultural heritage communication. However, in specific practical explorations, the media characteristics of digital technology and the remote participation of inherited community and other drawbacks are constantly exposed, resulting in the conflict between technology dominance and emotion loss in the digitalisation of intangible cultural heritage. Therefore, there is a need to reflect on the technology dominance of digitisation of intangible cultural heritage and to analyse the causes and effects of emotion drain. The paper also attempts to further propose measures that respect the cultural subjectivity of the inheriting community and focus on the emotional impact of digitisation, aiming to better realise the sustainable development of digitisation of intangible cultural heritage.
•Ultrasound washing was used with plasma treatment to disinfect blueberries.•Combined treatment showed higher microbial count reduction.•Combined treatment did not negatively affect the ...quality.•Combined treatment can activate antioxidant enzymes to scavenge ROS in blueberries.
Ultrasound (US) has limited disinfection efficacy, and it has been recommended to combine it with chemical disinfectants during fresh produce washing. After washing and before packaging, the disinfection effect of US-assisted washing can be weakened; thus, in-package disinfection is important. As a nutritious fruit, there are no packaged blueberries can be directly eaten. Therefore, in this study, blueberry was selected as the model, and the two most commonly used disinfectants (free chlorine FC at 10 ppm and peracetic acid PAA at 80 ppm) were combined with low-frequency US (25 kHz) during washing, followed by in-package disinfection using dielectric barrier discharge cold plasma (CP). The disinfection efficacy of US-FC and US-PAA against Escherichia coli O157:H7 and Salmonella Typhimurium was significantly higher than that of US, PAA, or FC alone. The highest disinfection efficacy of CP was observed at the pulse frequency range of 400–800 Hz. For US-FC (1 min) + CP (1 min), an additional 0.86, 0.71, 0.42, and 0.29 log CFU/g of reduction for E. coli O157:H7, S. Typhimurium, aerobic mesophilic counts, and mold and yeast was achieved, respectively, compared with US-FC (2 min) alone. For US-PAA (1 min) + CP (1 min) an additional 0.71, 0.59, 0.32, and 0.21 log CFU/g of reduction was achieved for the above organisms, respectively, compared with US-PAA (2 min) alone. Quality loss (in total color difference, firmness, and anthocyanin content) was not observed after treatment with US-FC + CP, US-PAA + CP, US-FC, or US-PAA. After treatment with US-FC + CP or US-PAA + CP, the reactive oxygen species (ROS) content was significantly lower than that in the other groups, and antioxidant enzyme activity was significantly higher than that in the other groups, suggesting that in-package CP can activate the blueberry antioxidant system to scavenge ROS, thereby lowering the risk of quality loss. US-CP combination not only improves the disinfection efficacy but also lowers quality loss caused by ROS, without prolonging the processing time.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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•A g-C3N4/TiO2 floating photocatalyst was prepared via a facile sol-gel method.•M. aeruginosa and Microcystin-LR were simultaneously removed under visible light.•Photoinduced holes ...and OH are the reactive species involved in photocatalysis.•Microcystin-LR was gradually degraded with oxidation of the Adda chain occurring.•The floating photocatalyst can be readily recovered for reuse.
Harmful algal blooms (HABs) have increasingly occurred worldwide causing human and ecological health risks. In this study, we report on an effective alternative to inactivating Microcystis aeruginosa under visible light irradiation with the use of a floating heterojunction photocatalyst. The photocatalyst (CTAE for short) is made of graphitic carbon nitride (g-C3N4) and TiO2 that are loaded on Al2O3-modified expanded perlite via a facile sol-gel method. The N/C doping in TiO2 and the interaction between TiO2 and g-C3N4 enhanced the generation of h+ and OH independent of O2 activation, which could facilitate applications in oxygen-deficient waters. Consideration was given to the impacts of different mass ratios of g-C3N4 to TiO2. The photocatalysts were characterized by XRD, N2 adsorption/desorption isotherms, FESEM/EDS, TEM, XPS, UV–vis absorption and Photoluminescence spectroscopy. Results showed that different contents of g-C3N4 had an influence on the crystal structure, specific surface area and pore volumes of the photocatalysts. The use of 2 g/L of 0.03CTAE (i.e., the theoretical mass ratio of g-C3N4 to TiO2 = 0.03) photocatalyst could simultaneously remove 88.1% of M. aeruginosa at an initial concentration of 2.7 × 106 cells/mL and 54.4% of Microcystin-LR at an initial concentration of 50 μg/L following 6-h of visible light irradiation. Microcystin-LR was gradually degraded with oxidation of the Adda chain occurring. Following each treatment, the floating g-C3N4/TiO2 photocatalyst could be readily recovered from the solution, demonstrating a great potential for in situ remediation of eutrophic waters.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
O-GlcNAcylation has been implicated in the tumorigenesis of various tissue origins, but its function in liver tumorigenesis is not clear. Here, we demonstrate that O-GlcNAcylation can enhance the ...expression, stability and function of Yes-associated protein (YAP), the downstream transcriptional regulator of the Hippo pathway and a potent oncogenic factor in liver cancer. O-GlcNAcylation induces transformative phenotypes of liver cancer cells in a YAP-dependent manner. An O-GlcNAc site of YAP was identified at Thr241, and mutating this site decreased the O-GlcNAcylation, stability, and pro-tumorigenic capacities of YAP, while increasing YAP phosphorylation. Importantly, we found via in vitro cell-based and in vivo mouse model experiments that O-GlcNAcylation of YAP was required for high-glucose-induced liver tumorigenesis. Interestingly, a positive feedback between YAP and global cellular O-GlcNAcylation is also uncovered. We conclude that YAP O-GlcNAcylation is a potential therapeutic intervention point for treating liver cancer associated with high blood glucose levels and possibly diabetes.
BackgroundThe utilization of long reads for single nucleotide polymorphism (SNP) phasing has become popular, providing substantial support for research on human diseases and genetic studies in ...animals and plants. However, due to the complexity of the linkage relationships between SNP loci and sequencing errors in the reads, the recent methods still cannot yield satisfactory results.ResultsIn this study, we present a graph-based algorithm, GCphase, which utilizes the minimum cut algorithm to perform phasing. First, based on alignment between long reads and the reference genome, GCphase filters out ambiguous SNP sites and useless read information. Second, GCphase constructs a graph in which a vertex represents alleles of an SNP locus and each edge represents the presence of read support; moreover, GCphase adopts a graph minimum-cut algorithm to phase the SNPs. Next, GCpahse uses two error correction steps to refine the phasing results obtained from the previous step, effectively reducing the error rate. Finally, GCphase obtains the phase block. GCphase was compared to three other methods, WhatsHap, HapCUT2, and LongPhase, on the Nanopore and PacBio long-read datasets. The code is available from https://github.com/baimawjy/GCphase.ConclusionsExperimental results show that GCphase under different sequencing depths of different data has the least number of switch errors and the highest accuracy compared with other methods.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Battling meat adulteration is essential in preserving both public health and a fair market. This study depicts a method of reducing adulteration in meats, in which Electronic-nose (E-nose) and gas ...chromatography-mass spectrometer (GC-MS) were applied to identify adulterants in mutton. Duck meat was selected as a model adulterant due to its lower cost and similarity in flavor to mutton, as well as being frequently adulterated in China. Qualitative and quantitative analysis were conducted using linear regression, fisher linear discriminant analysis (FLDA), and multilayer perceptron neural networks analysis (MLPN) on E-nose signals. Several fingerprint volatile organic chemicals (VOC) were identified by GC-MS to validate the E-nose results. Multivariate partial least square regression (PLS) was carried out to study the relationships between GC-MS and E-nose. The results of GC-MS confirmed that E-nose can be used to identify duck adulteration in mutton, with a minimum detection ratio of 10%. This method proved that rapid detection of mutton adulterated duck meat using E-nose has a high accuracy, which has reduced detecting time and improved detection efficiency.
•E-nose and GC-MS can be used to identify duck adulteration in mutton.•Detection of mutton adulterated duck meat using E-nose has a high accuracy.•E-nose detection was coupled with statistical analysis (MLPN, FLDA, PLS and LDA) for modeling.•E-nose can be used to identify duck adulteration in mutton, with a minimum detection ratio of 10%.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
•US-PAA + AA was more effective in controlling microbial growth than US-FC and US-CD.•US-PAA + AA did not induce additional quality loss.•US is a potential physical elicitor of phenolic content in ...fresh produce.
Traditional ultrasound (US)-assisted disinfection is only effective during washing. Coating is an effective method to control microbial growth after washing; however, cross-contamination can occur during immersion in the coating aqueous solution. Tap water (TW) rinsing is generally used to remove sanitizer residues after US-assisted washing; however, the Food and Drug Administration stated that rinsing is unnecessary when the peracetic acid (PAA) concentration does not exceed 80 ppm. In this study, we proposed a novel US-assisted hurdle technology of 80 ppm PAA combined with low-frequency US (25 kHz) during washing, followed by US-assisted aerosolization processing (nonimmersion coating). Ascorbic acid (AA), a safe and low-cost agent, was selected as the aerosolization solution. Cherry tomatoes were selected as the model, and the proposed method was compared with traditional US-assisted disinfection methods (US-10 ppm free chlorine washing + TW rinsing and US-5 ppm chlorine dioxide washing + TW rinsing) to analyze the disinfection efficacy and quality changes. During storage, US-PAA + 1%AA facilitated additional 0.7–0.9, 0.6–0.8, 0.7–1.0, and 0.5–1.0 log CFU/g reductions in the counts of Escherichia coli O157:H7, Salmonella Typhimurium, aerobic mesophilic counts, and molds and yeasts, respectively, as compared with traditional US-assisted methods. Sensory properties, color index, total soluble solids, titratable acidity, and weight loss were not negatively affected by any of the treatments. Firmness was slightly reduced after all treatments; however, the firmness of the samples was maintained during storage, in contrast with the decreased firmness observed in the control. Phenolic content and antioxidant activity significantly increased after all treatments. Further analysis of two key enzymes (phenylalanine ammonia-lyase and 4-coumarate-CoA ligase) involved in phenolic synthesis showed that their levels significantly increased following all treatments, leading to an increase in phenolic content and antioxidant activity. This result also indicated that US-assisted washing could act as an abiotic elicitor to increase nutritional content. Overall, US-PAA + 1%AA treatment served as an effective method for disinfecting produce during washing and for controlling microbial growth after washing without prolonging the processing time, which is an advantage over traditional US-assisted washing.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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