Abstract Delta opioid receptor (DOR) is essential for neuronal survival against hypoxic/ischemic damages. However, current understanding on how DOR activation affects astrocytic functions under ...ischemia remains incomplete. The present study investigated the astroglial responses to d -Ala2, d -Leu5 enkephalin (DADLE) (a selective DOR agonist)-induced DOR activation after global cerebral ischemia. Adult male rats were preimplanted with intracerebral cannula and subjected to global ischemia for 10 min. The rats were divided into four groups: normal group (without any procedure), sham group (sham procedure with intracerebroventricular injection of ACSF), I/R group (ischemia procedure with intracerebroventricular injection of ACSF) and DAD-treated group (ischemia procedure with intracerebroventricular injection of DADLE). Hippocampal CA1 neuronal survival and activation of astrocytes were measured in the animals at 72 h post-ischemia. The distribution and phenotypes of p-Akt and active caspase-3 were also determined. The ischemic injury resulted in a significant neuronal loss and an increase in the dying astrocytes in the hippocampal CA1 region as compared with those in the sham animals (200.7±22.7/mm2 vs. 6.6±3.1/mm2 , P <0.001). Improved neuronal survival in the DAD-treated animals was evident, which was accompanied by less dying astrocytes and enhanced astrocytes reaction with more active astrocytes than that in the I/R group (267.6±13.2/mm2 vs. 157.0±18.1/mm2 , P <0.01) and a significantly increased immunoreactivity of p-Akt. However, the active caspase-3 positive cells were also evident in DAD-treated group (313.0±23.1/mm2 ) and significantly increased as compared with those of the sham group (159.0±15.8/mm2 , P <0.001) or I/R group (193.6±26.2/mm2 , P <0.01). Most of the active caspase-3-expressing cells were colabeled with glial fibrillary acidic protein (GFAP), an astrocytes marker. We conclude that the post-ischemic treatment with DADLE promotes beneficial astrocytes activation and induces astroglial apoptosis 72 h after reperfusion which may be involved in reducing their harmful effect to neurons survival.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Electrospun polyacrylonitrile (PAN) nanofiber membrane was functionalized with chitosan and proteins for use in the treatment of dye-containing wastewater. The PAN nanofiber membrane was subjected to ...alkaline hydrolysis, before being grafted with chitosan and subsequently the proteins from chicken egg white. The resultant nanofiber membrane (P-COOH-CS-CEW) was comprehensively characterized using thermogravimetric analysis, Fourier-transform infrared spectroscopy, and scanning electron microscopy. The efficiency of P-COOH-CS-CEW in removing cationic dye toluidine blue O (TBO) and anionic dye acid orange 7 (AO7) in aqueous solution was evaluated. Based on the performance of model fitting, Langmuir and pseudo-second-order kinetic model could be used to describe the performance of P-COOH-CS-CEW in the removal of TBO (pH 10) and AO7 (pH 2) from the dye solutions. The adsorbed TBO and AO7 dyes can be completely desorbed by an elution solution made of 50% (v/v) ethanol and 1 M sodium chloride. After five consecutive adsorption-desorption cycles, the efficiency of dye removal by P-COOH-CS-CEW was maintained above 97%.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Summary The goal of this document is to update the original OARSI recommendations specifically for the design, conduct, and reporting of clinical trials that target symptom or structure modification ...among individuals with knee osteoarthritis (OA). To develop recommendations for the design, conduct, and reporting of clinical trials for knee OA we initially drafted recommendations through an iterative process. Members of the working group included representatives from industry and academia. After the working group members reviewed a final draft, they scored the appropriateness for recommendations. After the members voted we calculated the median score among the nine members of the working group who completed the score. The document includes 25 recommendations regarding randomization, blocking and stratification, blinding, enhancing accuracy of patient-reported outcomes (PRO), selecting a study population and index knee, describing interventions, patient-reported and physical performance measures, structural outcome measures, biochemical biomarkers, and reporting recommendations. In summary, the working group identified 25 recommendations that represent the current best practices regarding clinical trials that target symptom or structure modification among individuals with knee OA. These updated recommendations incorporate novel technologies (e.g., magnetic resonance imaging (MRI)) and strategies to address the heterogeneity of knee OA.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
A green and efficient method for producing high quality oil and defatted meal is in high demand, which has promoted the development of a hydration method for extracting oils from hem seed kernels. ...The hydration method optimized in this study recovered > 96% oil, which was further proved by infrared spectra, and extracted > 91% tocopherols, vitamin A, carotenoids, coenzyme Q10, phytosterols and squalene into the oil phase; while only small portions of flavonoids, other phenolic compounds and free fatty acids were extracted. The defatted meal was rich in water-soluble vitamins (including thiamin, riboflavin, niacin, pyridoxine, folate and vitamin C), proteins, dietary carbohydrates and phospholipids. The hydration method produced oils with lower AV or PV compared to solvent extraction, supercritical CO2 and cold-pressing methods and a defatted meal with a protein content (52.69%) which was significantly higher than that obtained by supercritical CO2 and cold-pressing methods. The hydration method is a type of green technology for sustainablly processing hempseeds.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Arbor Acres Plus male broilers (n = 1,152) were obtained at hatch and allocated to 1 of 6 diets from hatch to d 10 post-hatch. There were 16 replicate cages per diet and 12 birds per cage. Five of ...the diets were formulated to contain graded concentrations of standardized ileal digestible (SID) Ca at 0.60, 0.50, 0.40, 0.30, or 0.20%. A sixth reference diet was formulated using total Ca coefficients for each ingredient and contained 0.96% total Ca. Available P (avP) was maintained at 0.48% in all 6 diets. Data were subjected to an analysis of variance and the model included diet and block. Means were separated using contrasts to determine linear or quadratic effects of SID Ca and using the Dunnett's test to compare the reference diet to all SID Ca diets. There was no effect of graded levels of SID Ca on intake or gain. Birds fed diets containing 0.60, 0.50, 0.30, or 0.20% SID Ca ate (P < 0.05) or gained (P < 0.05) more compared with birds fed the reference diet. Mortality corrected FCR improved (linear, P < 0.05) as the SID Ca concentration in the diet increased from 0.20 to 0.60%. Tibia ash percent was greatest in birds fed 0.50% SID Ca and lowest in birds fed 0.20% SID Ca (quadratic, P < 0.05). Tibia ash percent was lower (P < 0.05) in birds fed diets formulated to contain 0.20% SID Ca compared with birds fed the reference diet. No other differences in tibia ash were reported. Apparent ileal digestibility (AID) or retention of P was greater (P < 0.05) in birds fed diets formulated using SID Ca compared with birds fed the reference diet. The AID of P increased (linear, P < 0.05) as the SID Ca content in the diet decreased from 0.60 to 0.20%. The AID or retention of Ca was similar in birds fed 0.60 or 0.50% SID Ca and increased as SID Ca decreased to 0.20% (quadratic, P < 0.05). Regression equations developed using bone ash and apparent P retention estimate the SID Ca requirement of Arbor Acres Plus broilers from hatch to d 10 post-hatch was 0.53 and 0.49%, respectively. This corresponds to a SID Ca to available P ratio of 1.1 to 1.02.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The numerical study of the fatigue behaviour of steel and light alloy substrates when coated with thin hard coatings is limited. This paper aims to investigate the fatigue failure mechanism of the ...coating system by observing the initiation and propagation of cracks within the coating under the cyclic loading. The model coating system is composed of three layers: the TiN coating, a case-hardened diffusion zone and the H11 steel substrate. The cohesive elements were arranged evenly in the horizontal direction and vertically through the thickness of the coating layer in order to observe the crack initiation and propagation. The model coating system was indented by a spherical indenter of 300 μm radius. Both the coating and the substrate were characterised as being homogenous, with elastic properties followed by linearly-hardening plastic behaviour. The irreversible cohesive zone model, allowing for the local degradation of the material properties to be incorporated into the model, was employed to simulate the crack initiation and propagation under cyclic loading. It was observed that the crack was initiated at the edge of the contact area between the indenter and coated surface at early stage of loading cycles, then progressed rapidly through the thickness of the coating layer. The deepest crack was found at 1.4 μm below the top surface. The study has demonstrated that the irreversible cohesive zone model can be used to track the evolution of crack propagation with cyclic loading, therefore has the capability to predict the loading bearing capacity of the coating system under contact fatigue loading conditions.
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•Local changes of material properties are incorporated in the model.•Damage variable captures progressive degradation of material.•Crack propagation dynamics are correlated with increasing loading cycles.•Evolution of deteriorating material properties is tracked within the contact area.•The model predicts surface loading bearing capacity under fatigue conditions.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Understanding future drought risks plays a crucial role in developing climate change adaptation strategies and in enhancing disaster resilience. However, previous studies may lead to biased ...conclusions due to the neglect of two factors, including the relative performance of climate simulations and the uncertainty in drought characterization. In this study, Bayesian model averaging is used to merge five regional climate model simulations and to project future changes in hydroclimatic regimes over China under two representative emission scenarios (RCP4.5 and RCP8.5). Drought characteristics, including drought severity and duration, are extracted using the Standardized Precipitation Evapotranspiration Index (SPEI). A Bayesian copula approach is used to uncover underlying interactions of drought characteristics and associated uncertainties across 10 climate divisions of China. The regional return periods of drought characteristics are used to assess future changes in multidimensional drought risks and the probability of extreme droughts. Our findings reveal that the variations in drought characteristics are generally underestimated by the ensemble mean (AEM) simulation. The Bayesian framework improves the reliability and accuracy of hydroclimate simulations and better reproduces the drought regimes compared to the AEM simulation. The drought duration and severity are projected to substantially increase for most areas of China based on the Bayesian framework, but the AEM simulation may lead to multiple opposite behaviors, especially under RCP4.5. The estimated joint risk from drought duration and drought severity is expected to increase under both emission scenarios. The likelihood of extreme droughts is also projected to increase as the radiative forcing increases.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, SIK, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Rotavirus remains a leading cause of diarrhoeal morbidity and mortality in young children and rotavirus vaccines are critical for reducing global disease burden. This report addresses the performance ...of rotavirus vaccines in countries with high child mortality. We performed a sensitivity analysis as part of a systematic review on rotavirus vaccines to inform development of World Health Organization vaccine recommendations. The efficacy of four prequalified vaccines against severe rotavirus gastroenteritis was similar across high mortality settings in Asia and Africa. Within the first year following vaccination, vaccine efficacy for the four vaccines ranged from 48% to 57% while in the second year, efficacy ranged from 29% to 54%. The four vaccines showed no increase in intussusception risk in these settings. All four vaccines appear to prevent significant numbers of severe rotavirus gastroenteritis episodes with no measurable increase in intussusception risk in high mortality settings in Africa and Asia.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Abstract Background With advances in immunosuppressive therapy, heart transplantation is currently recommended as the only established surgical treatment for refractory heart failure. However, ...chronic immunosuppression increases the risk for malignancy. Everolimus (EVR) is a potent mammalian target of rapamycin inhibitor that is used after transplantation and to treat advanced malignancies, as we have done in Taiwan after heart transplantation since 2004. Mycophenolate mofetil (MMF) and EVR are frequently used as cell-cycle inhibitors to optimize post-transplantation outcomes. Methods We retrospectively analyzed the characteristics and outcomes of 454 patients who received either MMF (n = 232) or EVR (n = 222) after heart transplantation at the National Taiwan University Hospital from March 1, 1990, to March 1, 2015. Patient characteristics and Kaplan-Meier survival curves were compared between groups. Results During a median follow-up of 69.2 months, malignancy was diagnosed in 27 patients receiving MMF (n = 23) or EVR (n = 4). There was a significant difference in malignancy risk between groups (9.91% vs 1.80%, P = .001). The most common malignancies were non-Hodgkin lymphoma, skin cancers, and lung squamous cell carcinoma. The 2-year overall survival after malignancy was 50% in the EVR group and 47% in the MMF group ( P = .745). Conclusions EVR treatment after heart transplant is associated with a lower risk of malignancy than is MMF treatment. The 2-year survival rate after malignancy was similar between EVR and MMF groups.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
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