Overexpression of soybean GmDREB1 in wheat confers drought tolerance in the field and is associated with increased melatonin content, an increased root system, and delayed leaf senescence.
Abstract
...Drought-response-element binding (DREB)-like transcription factors can significantly enhance plant tolerance to water stress. However, most research on DREB-like proteins to date has been conducted in growth chambers or greenhouses, so there is very little evidence available to support their practical use in the field. In this study, we overexpressed GmDREB1 from soybean in two popular wheat varieties and conducted drought-tolerance experiments across a range of years, sites, and drought-stress regimes. We found that the transgenic plants consistently exhibited significant improvements in yield performance and a variety of physiological traits compared with wild-type plants when grown under limited water conditions in the field, for example showing grain yield increases between 4.79–18.43%. Specifically, we found that the transgenic plants had reduced membrane damage and enhanced osmotic adjustment and photosynthetic efficiency compared to the non-transgenic controls. Three enzymes from the biosynthetic pathway of the phytohormone melatonin were up-regulated in the transgenic plants, and external application of melatonin was found to improve drought tolerance. Together, our results demonstrate the utility of transgenic overexpression of GmDREB1 to improve the drought tolerance of wheat in the field.
Accurate estimates of extreme precipitation events play an important role in climate change studies and natural disaster risk assessments. This study aimed to evaluate the capability of the China ...Meteorological Forcing Dataset (CMFD), Asian Precipitation-Highly Resolved Observational Data Integration Towards Evaluation of Water Resources (APHRODITE), and Climate Hazards Group Infrared Precipitation with Station data (CHIRPS) to detect the spatiotemporal patterns of extreme precipitation events over the Qinghai-Tibet Plateau (QTP) in China, from 1981 to 2014. Compared to the gauge-based precipitation dataset obtained from 101 stations across the region, 12 indices of extreme precipitation were employed and classified into three categories: fixed threshold, station-related threshold, and non-threshold indices. Correlation coefficient (CC), root mean square error (RMSE), mean absolute error (MAE), and Kling–Gupta efficiency (KGE), were used to assess the accuracy of extreme precipitation estimation; indices including probability of detection (POD), false alarm ratio (FAR), and critical success index (CSI) were adopted to evaluate the ability of gridded products’ to detect rain occurrences. The results indicated that all three gridded datasets showed acceptable representation of the extreme precipitation events over the QTP. CMFD and APHRODITE tended to slightly underestimate extreme precipitation indices (except for consecutive wet days), whereas CHIRPS overestimated most indices. Overall, CMFD outperformed the other datasets for capturing the spatiotemporal pattern of most extreme precipitation indices over the QTP. Although CHIRPS had lower levels of accuracy, the generated data had a higher spatial resolution, and with correction, it may be considered for small-scale studies in future research.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
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•Embelin treatment prior to TAA toxication can improve the survival of the mice.•Inflammatory cell infiltration occurred earlier in the embelin-treated recovery group.•Inflammatory ...cell reaction is consistent with hepatic healing process.
Embelin is an active component isolated from Embelia ribes Burm. In this study, we explored the protective effects of embelin on acute liver injury.
An animal model of acute liver injury was established via administration of a single injection of thioacetamide (TAA) (300 μg/g body weight) to adult mice. Embelin was administered by intragastric gavage at 50 μg/g body weight starting 2 days before TAA administration and continuing throughout the study. Survival of the mice was analyzed by the Kaplan-Meier method using the log-rank test. The acute liver injury protocol was repeated and the remaining mice were analyzed at indicated times. Hematoxylin and eosin staining and picrosirius red staining were used to examine necrosis/inflammation and liver healing, respectively. Liver function was assessed by serum alanine aminotransferase/alkaline phosphatase activity. Hepatic cleaved caspase-3 and F4/80 expression levels were examined via immunostaining. Statistical analysis was performed with GraphPad Software.
The survival and liver function of the mice were markedly better in the group treated with embelin prior to TAA toxication than in the TAA toxication-only group. Embelin significantly reduced TAA-induced hepatic necrosis/apoptosis. Massive inflammatory cell infiltration, which is consistent with hepatic fibrogenesis (a healing process), occurred earlier in the embelin-treated recovery group than in the spontaneous recovery group. Moreover, macrophage activities increased more rapidly with embelin treatment.
In summary, embelin can protect against acute liver injury. Its therapeutic value warrants further exploration.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Three-dimensional (3D) point cloud registration is an important step in three-dimensional (3D) model reconstruction or 3D mapping. Currently, there are many methods for point cloud registration, but ...these methods are not able to simultaneously solve the problem of both efficiency and precision. We propose a fast method of global registration, which is based on RGB (Red, Green, Blue) value by using the four initial point pairs (FIPP) algorithm. First, the number of different RGB values of points in a dataset are counted and the colors in the target dataset having too few points are discarded by using a color filter. A candidate point set in the source dataset are then generated by comparing the similarity of colors between two datasets with color tolerance, and four point pairs are searched from the two datasets by using an improved FIPP algorithm. Finally, a rigid transformation matrix of global registration is calculated with total least square (TLS) and local registration with the iterative closest point (ICP) algorithm. The proposed method (RGB-FIPP) has been validated with two types of data, and the results show that it can effectively improve the speed of 3D point cloud registration while maintaining high accuracy. The method is suitable for points with RGB values.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
This paper designs a scheme for a nursing professional teaching quality evaluation system, including constructing an evaluation index system and a nursing teaching quality evaluation model based on a ...fuzzy comprehensive evaluation. Among them, the establishment of a fuzzy comprehensive evaluation model involves the determination of fuzzy operators and the processing of evaluation indexes. Finally, the constructed method was used to conduct a study on evaluating nursing teaching quality in College H for students of different academic years. The results showed that freshman, sophomore, and junior students were inconsistent in all aspects of faculty teaching effectiveness and overall evaluations. The total rating of freshmen was 18.34±0.17. The total rating of sophomores was 20.50±0.15. The total rating of juniors was 23.46±0.12. And 66.7% of the faculty members thought that the quality of nursing teaching at the university was not up to standard. Therefore, this paper provides a valid assessment method for nursing education.
Neuroinflammation may induce a phenotype switch to reactive astrogliosis in neurodegenerative disorders. The calcium-activated potassium channel (KCa3.1) is active in the phenotypic switch that ...occurs during astrogliosis in Alzheimer's disease and ischemic stroke. Here, transcriptome sequencing (RNA-Seq), immunohistochemistry, western blotting, pharmacological blockade, and calcium imaging were used to investigate astrocyte KCa3.1 activity in neuroinflammation, Tau accumulation, and insulin signaling deficits in male wild-type C57BL/6 and KCa3.1−/− knockout (KO) mice, and in primary astrocyte cultures. KCa3.1 deficiency in KO mice decreased lipopolysaccharide (LPS)-induced memory deficits, neuronal loss, glial activation, Tau phosphorylation, and insulin signaling deficits in vivo. KCa3.1 expression in astrocytes was associated with LPS-induced upregulation of the Orai1 store-operated Ca2+ channel protein. The KCa3.1 channel was found to regulate store-operated Ca2+ overload through an interaction with Orai1 in LPS-induced reactive astrocytes. The LPS-induced effects on KCa3.1 and Orai1 indirectly promoted astrogliosis-related changes via the PI3K/AKT/GSK3β and NF-κB signaling pathways in vitro. Unbiased evaluation of RNA-Seq results for actively translated RNAs confirmed that substantial astrocyte diversity was associated with KCa3.1 deficiency. Our results suggest that KCa3.1 regulated astrogliosis-mediated neuroinflammation, Tau accumulation, and insulin signaling deficiency via PI3K/AKT/GSK3β and NF-κB signaling pathways, and contributing to neuronal loss and memory deficits in this neuroinflammation mouse model.
•Loss of KCa3.1 expression decreases LPS-induced memory deficiency and neuronal loss and glial activation•KCa3.1 and Orai1 are involved LPS-induced store-operated calcium entry (SOCE) in astrocytes•KCa3.1 and Orai1 are required for LPS-induced reactive astrogliosis and resultant neurotoxicity•RNA-Seq results confirmed that substantial astrocyte diversity was associated with KCa3.1 deficiency
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Production of pure lines is an important step in biological studies and breeding of many crop plants. The major types of pure lines for biological studies and breeding include doubled haploid (DH) ...lines, recombinant inbred lines (RILs), and near isogenic lines (NILs). DH lines can be produced through microspore and megaspore culture followed by chromosome doubling while RILs and NILs can be produced through introgressions or repeated selfing of hybrids. DH approach was developed as a quicker method than conventional method to produce pure lines. However, its drawbacks of genotype-dependency and only a single chance of recombination limited its wider application. A recently developed fast generation cycling system (FGCS) achieved similar times to those of DH for the production of selfed pure lines but is more versatile as it is much less genotype-dependent than DH technology and does not restrict recombination to a single event. The advantages and disadvantages of the technologies and their produced pure line populations for different purposes of biological research and breeding are discussed. The development of a concept of complete
meiosis and mitosis system is also proposed. This could integrate with the recently developed technologies of single cell genomic sequencing and genome wide selection, leading to a complete laboratory based pre-breeding scheme.
Ventilation air methane (VAM) is the main cause of greenhouse gas emissions in coal mining. Catalytic flow reverse reactor (CFRR) is widely used in VAM to mitigate methane emissions. In this study, ...palladium (Pd) and La1−xSrxMnO3 were used as catalysts in a CFRR. Different types of catalysts were prepared by loading La0.8Sr0.2MnO3, La0.9Sr0.1MnO3, and 0.1%Pd-La0.9Sr0.1MnO3 on a cordierite honeycomb reactor coated with γ-Al2O3 to compare their performances. In addition, this study compared the performance of the three catalysts in an 800 °C reactor based on different methane inlet concentrations, inlet speeds, and conversion times. The results showed: (1) 0.1% addition of Pd increased methane conversion. (2) La0.8Sr0.2MnO3 had higher efficiency at lower methane inlet concentrations, whereas La0.9Sr0.1MnO3 was more efficient at higher methane concentrations. This study demonstrates that a higher Sr loading is worth implementing only when the methane concentration of VAM is lower than 0.6%. (3) To achieve a higher methane conversion efficiency, the inlet velocity of methane should also be considered.
Drought severely limits sunflower production especially at the seedling stage. To investigate the response mechanism of sunflowers to drought stress, we utilized two genotypes of sunflower materials ...with different drought resistances as test materials. The physiological responses were investigated under well-watered (0 h) and drought-stressed conditions (24 h, 48 h, and 72 h).
ANOVA revealed the greatest differences in physiological indices between 72 h of drought stress and 0 h of drought stress. Transcriptome analysis was performed after 72 h of drought stress. At 0 h, there were 7482 and 5627 differentially expressed genes (DEGs) in the leaves of K55 and K58, respectively, and 2150 and 2527 DEGs in the roots of K55 and K58, respectively. A total of 870 transcription factors (TFs) were identified among theDEGs, among which the high-abundance TF families included AP2/ERF, MYB, bHLH,and WRKY. Five modules were screened using weighted gene coexpressionnetwork analysis (WGCNA), three and two of which were positively and negatively, respectively, related to physiological traits. KEGG analysis revealedthat under drought stress, "photosynthesis", "carotenoid biosynthesis", "starch and sucrose metabolism", "ribosome", "carotenoid biosynthesis", "starch and sucrose metabolism", "protein phosphorylation" and "phytohormone signaling" are six important metabolic pathways involved in the response of sunflower to drought stress. Cytoscape software was used to visualize the three key modules, and the hub genes were screened. Finally, a total of 99 important candidate genes that may be associated with the drought response in sunflower plants were obtained, and the homology of these genes was compared with that in Arabidopsis thaliana.
Taken together, our findings could lead to a better understanding of drought tolerance in sunflowers and facilitate the selection of drought-tolerant sunflower varieties.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Deoxynivalenol (DON), a common mycotoxin of type B trichothecene, is produced mainly by several
species. DON causes great losses in farming and poses severe safety risks to human and animal health. ...Thus, DON contamination in cereals and DON toxicity are of worldwide concern. In this study, we screened the bacterial consortium C20, which efficiently degraded almost 70 μg ml
DON within 5 days. The bacterial consortium also had the ability to degrade 15-acetyl-DON, 3-acetyl-DON, and T-2 toxin. The bacterial consortium C20 was able to degrade DON under a wide range of pH and temperature conditions. The optimal temperature and pH for DON degradation were 30°C and pH 8.0, respectively. The bacterial consortium C20 comprised of different bacterial genera, and several strains were found to significantly increase when cultured in Mineral Medium with 100 μg ml
DON based on the analysis of the sequences of the hypervariable V3-V4 region of the 16S rRNA gene. 3-keto-DON was confirmed as a degradation product of DON by liquid chromatography/time-of-flight/mass spectrometry (LC-TOF-MS) and nuclear magnetic resonance (NMR) analyses. The results indicated that the bacterial consortium C20 is a potential candidate for the biodegradation of DON in a safe and environmentally friendly manner.
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