Elevated intraocular pressure (IOP) is the leading cause of glaucoma. As glaucoma is an irreversible neurological eye disease, it is urgent to realize timely and accurate IOP detection for diagnostic ...and therapeutic purposes. Here, a contact lens sensor for continuous IOP monitoring using self‐assembly graphene (SAG), is developed. The combination of face‐to‐face water transfer printing and micro‐electromechanical systems technology can realize the batch preparation of such a sensor. The sensor has good light transmittance and temperature stability. It exhibits an ultra‐high IOP sensitivity of 1.0164 mV mm Hg−1 on a silicone eye, and 3.166 mV mm Hg−1 in vitro on the porcine eye with remarkable linearity. The sensitivity of the proposed sensor is high enough to be read by a commercial radio frequency identification read–write system for continuous wireless monitoring of IOP. Furthermore, the as‐prepared sensor can work as normal for 24 h in phosphate buffer saline.
The non‐invasive intraocular pressure (IOP) sensor, which combines a “fish scale”‐like graphene structure and an optimized Wheatstone bridge circuit, exhibits an unprecedented IOP sensitivity of 1.0164–3.166 mV mmHg–1 in vitro. The voltage response is high enough to be detected over a commercial wireless system. A face‐to‐face water transferring method is developed to realize batch production of the sensor.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
The historical trajectory detection plays a vital role and has the practical significance for commercial user behavior analysis, the identity of the police suspect landing and surveillance of key. ...Current detection methods use mobile client silence to collect user information, or use operators to shunt data, or shooting combined with artificial analysis. The ways have many disadvantages such as the sample is limited, the effective information is difficult to extract and the analytical efficiency is low. A historical track detection based on Wi-Fi protocol was proposed, and specific system design and function relization were given which provided a new way to analyze the behavior of users.
Background
The reduction and fixation of Medial humeral calcar is difficult in the treatment of elderly proximal humerus Neer 3 and 4-part fractures with a single lateral locking plate. Our study ...investigated the efficacy of an intramedullary calcar supporting plate combined with a lateral locking plate for the treatment of 3- and 4-part fractures of the proximal humerus in the elderly through a deltoid splitting approach.
Methods
From June 2022 to December 2022, we treated six elderly patients with Neer 3 and 4-part fractures using proximal humeral intramedullary calcar support plate in combination with lateral locking plate through a deltoid splitting approach. Follow-up time was 6–12 months. Assessment indicators included fracture union, quality of reduction, and complication rate. The Constant-Murley score was used to record shoulder function at 6 months postoperatively.
Results
All 6 patients showed fracture union and anatomic reduction. Constant-Murley score was 79.5 (70–90) at 6 months postoperatively. There was no incision non-healing, internal fixation failure, bone non-union or surgical site infection, secondary surgery, or death. Shoulder impingement occurred in 1 case.
Conclusion
Proximal humeral intramedullary calcar support plate combined with lateral locking plate fixation through a deltoid splitting approach can effectively maintain fracture reduction, prevent inversion collapse of humeral head and internal fixation failure, and provide satisfactory clinical results at an early stage.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Inner filter effect (IFE), a well-known phenomenon of fluorescence quenching resulting from absorption of the excitation or emission light of luminescent species by absorbent, has been used as a ...smart approach to design fluorescent sensors, which are characterized by the simplicity and flexibility with high sensitivity. However, further application of IFE-based sensors in complex environment is hampered by the insufficient IFE efficiency and low sensitivity resulting from interference of the external environment. In this paper, we report that IFE occurring on a solid substrate surface would solve this problem. As a proof of concept, a fluorescent sensor for intracellular biothiols has been developed on the basis of the absorption of a newly designed thiols-specific chromogenic probe (CP) coupled with the use of a thiols-independent fluorophore, rhodamine 6G (R6G), operative on the IFE on graphene oxide (GO). To construct an efficient IFE system, R6G was covalently attached to GO, and the CP molecules were adsorbed on the surface of R6G-GO via π–π stacking interaction. The reaction of thiols with CP on R6G-GO decreases the absorption of CP, resulting in the increase of the intensity of R6G fluorescence. The results showed that the IFE efficiency, sensitivity, and dynamic response time of R6G-GO/CP for biothiols could be significantly improved compared with R6G/CP, and furthermore, R6G-GO/CP functioned under complex system and could be used for assaying biothiols in living cells and in human serum samples. This new strategy would be general to explore the development of more effective IFE-based sensors for other analytes of interest.
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IJS, KILJ, NUK, PNG, UL, UM
Soil salinity influences various aspects of crop growth and development. Identification of novel germplasm and genetic regulators of salt tolerance will help to alleviate the threat of land ...salinization. Here, we identified salt-tolerant SHEN5003 and -sensitive YZ26 maize germplasm from different heterotic groups. Salt-grown SHEN5003 (PA group) lines exhibited decreased reduction in fresh weight and dry weight, high SPAD value, total sugar and K
+
contents, as well as low Na
+
content relative to salt-treated YZ26 (BSSS group) plants. Salt-tolerant SHEN5003 and -sensitive YZ26 lines were followingly used for the discovery of genetic regulators of salt tolerance. Transcriptome outcome showed the expression of genes relevant to Na
+
/K
+
homeostasis, such as
H
+
-ATPase
,
HKT1
, and
HAKs
together with
CAT2
for ROS detoxification was altered in salt-treated SHEN5003 line. Moreover, GO enrichment analysis indicated differentially expressed genes of SHEN5003 upon salt treatment was significantly enriched in the stress response pathway (GO:0006950). By integrated transcriptome analyses of SHEN5003 and YZ26 lines grown in normal and salinity conditions, positive regulator of salt tolerance
ZmSWEET1b
was revealed, which exhibited the opposite expression pattern between salt-treated SHEN5003 and YZ26 lines.
ZmSWEET1b
interacted with multiple partners, such as
Sucrose synthase 2
to shape a functional association network in the modulation of sugar metabolism upon salinity stress.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
BACKGROUNDTriple-negative breast carcinomas (TNBCs) are a breast carcinoma with the most aggressive form, which is demonstrated as enhanced invasion and recurrence. Britannin is extracted mainly from ...the traditional Chinese herb Inula japonica Thunb, and few studies have focused on its effect on TNBC. Moreover, there is still no report concerning the role of Britannin in degrading the transcripts of Zinc finger E-box-binding homeobox 1 (ZEB1) proteins. PURPOSETo explore the potential effect of Britannin on invasion and stemness of TNBCs and its underlying mechanism. METHODSCellular activity was measured using MTT, and cell cycle was measured using flow cytometry (FCM). The effect of Britannin on the migrating and invading abilities of MDA-MB-231 and 4T1 cells were measured using the wound healing and transwell assays. The sizes and number of breast carcinoma cells were measured by tumor formation assay and in vitro limiting-dilution assay. CD44 expression in tumor spheroids was tested by immunofluorescence assay. Nextly, the expressions of epithelial-mesenchymal transition (EMT) markers and ZEB1 protein expressional level were detected by western blot . ZEB1 mRNA expressional level was analyzed using RT-qPCR. Drug affinity-responsive target stability (DARTS) method was used to detect the binding activity between Britannin and ZEB1. Co-immunoprecipitation (Co-IP) analysis was applied to test the ubiquitination of ZEB1. The mouse models for experimental lung metastasis of 4T1 cells were established to detect the anti-metastasis effect of Britannin in vivo, and the expressional levels of EMT markers in lung metastases were detected by immunohistochemistry. RESULTSBritannin could inhibit cell growth and G2/M arrest in TNBC cells. Britannin could inhibit the migrating and invading ability without inducing severe apoptosis of MDA-MB-231 and 4T1 cells. Meanwhile, Britannin reduced the size and number of spheroids formed in these two cells, and decreased the expressional level of stem cells biomarker CD44 in tumor spheroids. Mechanism research showed that Britannin specifically bound to ZEB1 and induced its ubiquitination in MDA-MB-231 cells. Afterwards, Britannin disturbed protein stability and promoted ZEB1 protein degradation. Importantly, Britannin could not inhibit cell invasion and spheroid formation after ZEB1 expression was knocked down. Finally, Britannin inhibition of 4T1 cell metastasis was confirmed through establishing mouse models for the experimental lung metastasis. It was proved that both Britannin and paclitaxel could decrease the lung metastases, and Britannin could also down-regulate the protein expressional levels of ZEB1, MMP9 and CD44. CONCLUSIONThis study reveals that Britannin suppresses the invasion and metastasis of TNBC cells through degrading ZEB1, which suggests that Britannin can be used to prevent tumor metastasis and recurrence via degrading ZEB1proteins.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Matrix metalloproteinase-9 (MMP-9) is an attractive target for cancer therapy. In this study, the pharmacophore model of MMP-9 inhibitors is built based on the experimental binding structures of ...multiple receptor-ligand complexes. It is found that the pharmacophore model consists of six chemical features, including two hydrogen bond acceptors, one hydrogen bond donor, one ring aromatic regions, and two hydrophobic (HY) features. Among them, the two HY features are especially important because they can enter the S1′ pocket of MMP-9 which determines the selectivity of MMP-9 inhibitors. The reliability of pharmacophore model is validated based on the two different decoy sets and relevant experimental data. The virtual screening, combining pharmacophore model with molecular docking, is performed to identify the selective MMP-9 inhibitors from a database of natural products. The four novel MMP-9 inhibitors of natural products, NP-000686, NP-001752, NP-014331, and NP-015905, are found; one of them, NP-000686, is used to perform the experiment of in vitro bioassay inhibiting MMP-9, and the IC
50
value was estimated to be only 13.4 µM, showing the strongly inhibitory activity of NP-000686 against MMP-9, which suggests that our screening results should be reliable. The binding modes of screened inhibitors with MMP-9 active sites were discussed. In addition, the ADMET properties and physicochemical properties of screened four compounds were assessed. The found MMP-9 inhibitors of natural products could serve as the lead compounds for designing the new MMP-9 inhibitors by carrying out structural modifications in the future.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
A novel self-excited atmospheric pressure microwave plasma jet (APMPJ) based on a coaxial line is successfully proposed for the medical treatment. The finite-element method (FEM) is applied to obtain ...the optimal size of the APMPJ. The plasma parameters, such as electron density and electron temperature, are calculated within 0.1 ms. When the plasma reaches a stable state, the electron density is closed to 1022 m−3, and the electron temperature is calculated to be 1.3 eV. According to the experimental results, the argon plasma jet can be self-excited at an incident power of 20 W, and the gas temperature of the plasma jet is closed to 318 K at an incident power of 15 W. In addition, the air plasma jet can be self-excited at an incident power of 110 W. Finally, the change in the density ratio of hydroxyl (OH) radical to other particles when water vapor is added to the APMPJ at different flow rates is analyzed. It is found that a small amount of water vapor can greatly increase the relative content of OH which is tested by spectrometer.
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