Intestinal commensal bacteria can inhibit dense colonization of the gut by vancomycin-resistant Enterococcus faecium (VRE), a leading cause of hospital-acquired infections
. A four-strained ...consortium of commensal bacteria that contains Blautia producta BP
can reverse antibiotic-induced susceptibility to VRE infection
. Here we show that BP
reduces growth of VRE by secreting a lantibiotic that is similar to the nisin-A produced by Lactococcus lactis. Although the growth of VRE is inhibited by BP
and L. lactis in vitro, only BP
colonizes the colon and reduces VRE density in vivo. In comparison to nisin-A, the BP
lantibiotic has reduced activity against intestinal commensal bacteria. In patients at high risk of VRE infection, high abundance of the lantibiotic gene is associated with reduced density of E. faecium. In germ-free mice transplanted with patient-derived faeces, resistance to VRE colonization correlates with abundance of the lantibiotic gene. Lantibiotic-producing commensal strains of the gastrointestinal tract reduce colonization by VRE and represent potential probiotic agents to re-establish resistance to VRE.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Resistance to neoadjuvant chemotherapy leads to poor prognosis of locally advanced rectal cancer (LARC), representing an unmet clinical need that demands further exploration of therapeutic strategies ...to improve clinical outcomes. Here, we identified a noncanonical role of RB1 for modulating chromatin activity that contributes to oxaliplatin resistance in colorectal cancer (CRC). We demonstrate that oxaliplatin induces RB1 phosphorylation, which is associated with the resistance to neoadjuvant oxaliplatin-based chemotherapy in LARC. Inhibition of RB1 phosphorylation by CDK4/6 inhibitor results in vulnerability to oxaliplatin in both intrinsic and acquired chemoresistant CRC. Mechanistically, we show that RB1 modulates chromatin activity through the TEAD4/HDAC1 complex to epigenetically suppress the expression of DNA repair genes. Antagonizing RB1 phosphorylation through CDK4/6 inhibition enforces RB1/TEAD4/HDAC1 repressor activity, leading to DNA repair defects, thus sensitizing oxaliplatin treatment in LARC. Our study identifies a RB1 function in regulating chromatin activity through TEAD4/HDAC1. It also provides the combination of CDK4/6 inhibitor with oxaliplatin as a potential synthetic lethality strategy to mitigate oxaliplatin resistance in LARC, whereby phosphorylated RB1/TEAD4 can serve as potential biomarkers to guide the patient stratification.
Vestibular-evoked myogenic potentials (VEMPs) are important methods for evaluating vestibular otolith conduction pathway. Air-conducted sound (ACS) cervical/ocular vestibular-evoked myogenic ...potentials (cVEMPs/oVEMPs) have been widely used in clinic, bone-conduction vibration stimulation (BCV)- cVEMPs/oVEMPs also began to be used in clinic, but galvanic vestibular stimulation (GVS)-cVEMPs/oVEMPs have not been reported in China. This article reviews the detection and clinical application of GVS-VEMPs.
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FFLJ, NUK, ODKLJ, UL, UM, UPUK, VSZLJ
A novel influenza A (H1N1) has been spreading worldwide. Early studies implied that international air travels might be key cause of a severe potential pandemic without appropriate containments. In ...this study, early outbreaks in Mexico and some cities of United States were used to estimate the preliminary epidemic parameters by applying adjusted SEIR epidemiological model, indicating transmissibility infectivity of the virus. According to the findings, a new spatial allocation model totally based on the real-time airline data was established to assess the potential spreading of H1N1 from Mexico to the world. Our estimates find the basic reproductive number R0 of H1N1 is around 3.4, and the effective reproductive number fall sharply by effective containment strategies. The finding also implies Spain, Canada, France, Panama, Peru are the most possible country to be involved in severe endemic H1N1 spreading.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
A CO2 laser-based phase contrast imaging (PCI) diagnostic has been developed on HL-2A tokamak. It can detect line integrated plasma density fluctuations by measuring the phase shift of laser beam ...after being scattered by the bulk plasma. The diagnosed radial region ranges from r a = 0.625 to 0.7. 32-channel HgCdTe detectors with alternative-current biased amplifiers are arranged in line at the imaging plane of the optical path. This PCI is able to diagnose density fluctuations with wavenumbers ranging from 2 to 15 cm−1 and the time resolution is better than 2 s. The first experimental data were achieved in 2018 spring campaign of HL-2A tokamak. High performance is confirmed in different discharging configurations and makes it a keen tool in broadband turbulence investigations.
Objective The efficacy of the two teaching methods for EBUS-TBNA was evaluated by comparing the learning curve, diagnostic accuracy, and specimen acquisition for genotyping by operators and so to ...find a better one for training pulmonologists. Methods The first fifty patients of each operator learning EBUS-TBNA between January 2015 and June 2019 in a single center were retrospective collected and grouped by the operators training methods. The diagnosis, genotyping and clinical characters were reviewed. Results A total of 442 patients were collected and reviewed. There were two training groups: a conventional training group (232 cases) and a simulator assistant training group (210 cases). There was no statistical difference in the diagnostic yield between the groups of trainees. However, specimen from lung cancer patients in simulator training group were more adequate for gene testing than in conventional training group (86.8% vs 71.2%, P＜0.05). By comparing the learning curve among the groups, it was found tha
The silver sillago, Sillago sihama Forsskl is naturally distributed in IndoWest Pacific: Red Sea and Knysna, South Africa to Japan and south to Australia, and is common along beaches, sandbars, ...mangrove creeks and estuaries. Adults bury themselves in the sand when disturbed, and feed mainly on polychaete worms, small prawns (Penaeus), shrimps and amphipods. This fish is found in all Chinese waters including East China Sea, Yellow Sea, Bohai Sea, South China Sea, and the Northern Bay. In the present study, we developed 66 microsatellite loci isolated from a dinucleotide-enriched genomic library of S. sihama using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol of Zane et al. (2002). These markers should provide a tool for assessing genetic diversity and population structure of wild stocks.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
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