Heterosis is a phenomenon in which hybrids exhibit superior performance relative to parental phenotypes. In addition to the heterosis of above-ground agronomic traits on which most existing studies ...have focused, root heterosis is also an indispensable component of heterosis in the entire plant and of major importance to plant breeding. Consequently, systematic investigations of root heterosis, particularly in reproductive-stage rice, are needed. The recent advent of RNA sequencing technology (RNA-Seq) provides an opportunity to conduct in-depth transcript profiling for heterosis studies.
Using the Illumina HiSeq 2000 platform, the root transcriptomes of the super-hybrid rice variety Xieyou 9308 and its parents were analyzed at tillering and heading stages. Approximately 391 million high-quality paired-end reads (100-bp in size) were generated and aligned against the Nipponbare reference genome. We found that 38,872 of 42,081 (92.4%) annotated transcripts were represented by at least one sequence read. A total of 829 and 4186 transcripts that were differentially expressed between the hybrid and its parents (DGHP) were identified at tillering and heading stages, respectively. Out of the DGHP, 66.59% were down-regulated at the tillering stage and 64.41% were up-regulated at the heading stage. At the heading stage, the DGHP were significantly enriched in pathways related to processes such as carbohydrate metabolism and plant hormone signal transduction, with most of the key genes that are involved in the two pathways being up-regulated in the hybrid. Several significant DGHP that could be mapped to quantitative trait loci (QTLs) for yield and root traits are also involved in carbohydrate metabolism and plant hormone signal transduction pathways.
An extensive transcriptome dataset was obtained by RNA-Seq, giving a comprehensive overview of the root transcriptomes at tillering and heading stages in a heterotic rice cross and providing a useful resource for the rice research community. Using comparative transcriptome analysis, we detected DGHP and identified a group of potential candidate transcripts. The changes in the expression of the candidate transcripts may lay a foundation for future studies on molecular mechanisms underlying root heterosis.
Full text
Available for:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
A depth-averaged two-dimensional (2D) numerical model for unsteady flow and nonuniform sediment transport in open channels is established using the finite volume method on a nonstaggered, curvilinear ...grid. The 2D shallow water equations are solved by the SIMPLE(C) algorithms with the Rhie and Chow's momentum interpolation technique. The proposed sediment transport model adopts a nonequilibrium approach for nonuniform total-load sediment transport. The bed load and suspended load are calculated separately or jointly according to sediment transport mode. The sediment transport capacity is determined by four formulas which are capable of accounting for the hiding and exposure effects among different size classes. An empirical formula is proposed to consider the effects of the gravity on the sediment transport capacity and the bed-load movement direction in channels with steep slopes. Flow and sediment transport are simulated in a decoupled manner, but the sediment module adopts a coupling procedure for the computations of sediment transport, bed change, and bed material sorting. The model has been tested against several experimental and field cases, showing good agreement between the simulated results and measured data.
Full text
Available for:
DOBA, FGGLJ, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
AbstractA depth-averaged two-dimensional model has been developed in this study to simulate the unsteady flow and noncohesive sediment transport due to embankment break and overtopping breaching. The ...model adopts the generalized shallow-water equations that consider the effects of sediment transport and bed change on the flow, thus leading to coupled calculations of these processes. It computes the non-equilibrium total-load sediment transport and considers the noncohesive embankment slope avalanching. The model solves the governing equations using an explicit finite-volume method on a rectangular grid, with the Harten, Lax and van Leer (HLL) approximate Riemann solver to handle the mixed-regime flows generated by embankment break/breaching and the monotonic upstream scheme for conservation laws (MUSCL) piecewise reconstruction method to reach second-order accuracy in space. It uses a varying time step length that satisfies both the Courant-Friedrichs-Lewy condition and the limitation that the bed change is less than about ten percent of the local flow depth at each time step. Validations using laboratory and field experiments showed that the developed model generally predicts well the embankment-break wave propagation over movable beds, the induced sediment transport and bed changes, and the temporal evolution of noncohesive embankment breach.
Full text
Available for:
DOBA, FGGLJ, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
This study focuses on the prediction of the porosity of nonuniform sediment mixtures, considering the effects of particle packing. A random particle packing model has been developed for the porosity ...of bimodal mixtures by extending the existing random particle filling theory. Coefficients in the developed model are calibrated by fitting the model to measured data for a variety of bimodal mixtures, including spherical glass particles, rounded quarry grains, and natural sediments. The model coefficients are found to be functions of the diameter ratio of coarse and fine particles, separately for spherical glass particles and natural sediments due to differences in particle shapes. The bimodal mixture model is then extended to trimodal particle mixtures in three approaches: two based on serial packing and one based on random packing. These models are tested against experimental data of six trimodal mixtures with spherical glass beads. The results show that the random packing model and the fine–medium–coarse particle serial packing model predict well the observed trimodal mixture porosities.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
The porosity of a bimodal sediment mixture is affected by the filling of fine particles in the voids of coarse particles when the particle size range is wide. The classical ideal packing models tend ...to overestimate the filling, and, thus, underestimate the porosity of the mixture. In this study, an existing random filling model is improved by considering a three-dimensional packing configuration, and a new model is developed by considering how many fine particles are required and how many are available to cover the surface of coarse particles in the sediment mixture. The developed models are validated using measured data and compared with existing models. The new model can reproduce well the variation trend of the mixture porosity as the fraction of fine particles varies.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Empirical formulas have been developed to calculate the fractional bed-load and suspended-load transport rates and near-bed suspended-load concentration under non-breaking waves and currents for ...coastal applications. The formulas relate the bed-load transport rate to the grain shear stress, the suspended-load transport rate to the energy of the flow system, and the near-bed suspended-load concentration to the bed-load transport rate, velocity and layer thickness. Adequate methods are adopted to determine the bed shear stress due to coexisted waves and currents. The hiding and exposure mechanism in nonuniform bed material is considered through a correction factor that is related to the hiding and exposure probabilities and in turn the size composition of bed material. The developed formulas have been tested using a large database of single-sized sediment transport and several sets of multiple-sized sediment transport data collected from literature, and compared with several existing formulas. The developed formulas can provide reasonably good predictions for the test cases.
•We developed a formula for multiple-sized bed-load transport rate.•We developed a formula for multiple-sized suspended-load transport rate.•We developed a formula for multiple-sized near-bed suspended-load concentration.•We tested the developed formulas using a large number of lab and field data.•We compared the developed formulas with several existing formulas.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Highly efficient photocatalytic reduction of 4-nitroaniline to p-phenylenediamine over a commercial CdS photocatalyst was observed under visible light irradiation ( lambda greater than or equal to ...420 nm) in water. The conversion of 4-nitroaniline and the selectivity of p-phenylenediamine were similar to 100% and similar to 98% after 9 min of visible light irradiation, respectively. The photoreduction efficiency of 4-nitroaniline over the CdS photocatalyst remained above 95% in the 5th cycle of testing. Its photocatalytic activity was much higher than those of nitrogen-doped TiO sub(2) and commercial TiO sub(2) photocatalysts. Further experimental results revealed that the ammonium formate and N sub(2) atmosphere were indispensable for the photocatalytic reduction of 4-nitroaniline over the CdS photocatalyst. On the basis of the results of electron spin resonance, photoexcited electrons and .CO sub(2) super(-) radicals were detected in the present system. These species had strong reductive powers, and were therefore able to efficiently reduce 4-nitroaniline to p-phenylenediamine.
Osteoporosis is a common metabolic skeletal disease and usually lacks obvious symptoms. Many individuals are not diagnosed until osteoporotic fractures occur. Bone mineral density (BMD) measured by ...dual-energy X-ray absorptiometry (DXA) is the gold standard for osteoporosis detection. However, only a limited percentage of people with osteoporosis risks undergo the DXA test. As a result, it is vital to develop methods to identify individuals at-risk based on methods other than DXA.
We proposed a hierarchical model with three layers to detect osteoporosis using clinical data (including demographic characteristics and routine laboratory tests data) and CT images covering lumbar vertebral bodies rather than DXA data via machine learning. 2210 individuals over age 40 were collected retrospectively, among which 246 individuals' clinical data and CT images are both available. Irrelevant and redundant features were removed via statistical analysis. Consequently, 28 features, including 16 clinical data and 12 texture features demonstrated statistically significant differences (p < 0.05) between osteoporosis and normal groups. Six machine learning algorithms including logistic regression (LR), support vector machine with radial-basis function kernel, artificial neural network, random forests, eXtreme Gradient Boosting and Stacking that combined the above five classifiers were employed as classifiers to assess the performances of the model. Furthermore, to diminish the influence of data partitioning, the dataset was randomly split into training and test set with stratified sampling repeated five times. The results demonstrated that the hierarchical model based on LR showed better performances with an area under the receiver operating characteristic curve of 0.818, 0.838, and 0.962 for three layers, respectively in distinguishing individuals with osteoporosis and normal BMD.
The proposed model showed great potential in opportunistic screening for osteoporosis without additional expense. It is hoped that this model could serve to detect osteoporosis as early as possible and thereby prevent serious complications of osteoporosis, such as osteoporosis fractures.
Full text
Available for:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
This paper presents a depth-averaged two-dimensional shallow water model for simulating long waves in vegetated water bodies under breaking and non-breaking conditions. The effects of rigid ...vegetation are modelled in the form of drag and inertia forces as sink terms in the momentum equations. The drag coefficient is treated as a calibrated bulk constant and also determined using two empirical formulas as functions of stem Reynolds number, Froude number, and vegetation volume fraction. The governing equations are solved using an explicit finite-volume method based on rectangular mesh with the Harten, Lax, and van Leer approximate Riemann solver with second-order piecewise linear reconstruction for the streamwise convection fluxes, a second-order upwind scheme for the lateral convection fluxes, and a stable centred difference scheme for the water surface gradient terms. The model was tested using five laboratory experiments, including steady flow in a flume with alternate vegetation zones, solitary wave in a vegetated flatbed flume, long-wave runup on a partially-vegetated sloping beach, the dam-break wave overtopping an obstacle, and breaking the solitary wave on a sloping beach. The computed water levels, flow velocities, wave heights, and runups are in generally good agreement with experimental observations. The model was then applied to assess the hydrodynamic effectiveness and limitations of vegetation in coastal and river protection. It is shown that vegetation along the coastal shoreline has a positive benefit in reducing wave runup on sloping beaches, whereas vegetation in open channels causes conflicting impacts: reducing inundation in the downstream areas, but increasing flood risk in a certain distance upstream.
Full text
Available for:
BFBNIB, GIS, IJS, KISLJ, NUK, PNG, UL, UM, UPUK
Obesity is a public health crisis, presenting a huge burden on health care and the economic system in both developed and developing countries. According to the WHO’s latest report on obesity, 39% of ...adults of age 18 and above are obese, with an increase of 18% compared to the last few decades. Metabolic energy imbalance due to contemporary lifestyle, changes in gut microbiota, hormonal imbalance, inherent genetics, and epigenetics is a major contributory factor to this crisis. Multiple studies have shown that probiotics and their metabolites (postbiotics) supplementation have an effect on obesity-related effects in vitro, in vivo, and in human clinical investigations. Postbiotics such as the SCFAs suppress obesity by regulating metabolic hormones such as GLP-1, and PPY thus reducing feed intake and suppressing appetite. Furthermore, muramyl di-peptides, bacteriocins, and LPS have been tested against obesity and yielded promising results in both human and mice studies. These insights provide an overview of targetable pharmacological sites and explore new opportunities for the safer use of postbiotics against obesity in the future.