With the large-scale application of lithium-ion batteries (LIBs), a huge amount of spent LIBs will be generated each year and how to realize their recycling and reuse in a clean and effective way ...poses a challenge to the society. In this work, using the electrolyte of spent LIBs as solvent, we in situ fluorinate the conductive three-dimensional porous copper foam by a facile solvent-thermal method and then coating it with a cross-linked sodium alginate (SA) layer. Benefiting from the solid-electrolyte interphase (SEI) that accommodating the volume change of internal CuF2 core and SA layer that inhibiting the dissolution of CuF2, the synthesized CuF2@void@SEI@SA cathode with a pomegranate-like structure (yolk-shell) exhibits a large reversible capacity of ~535 mAh g-1 at 0.05 A g-1 and superb cycling stability. This work conforms to the development concept of green environmental protection and comprehensively realizes the unity of environmental, social and economic benefits.With the large-scale application of lithium-ion batteries (LIBs), a huge amount of spent LIBs will be generated each year and how to realize their recycling and reuse in a clean and effective way poses a challenge to the society. In this work, using the electrolyte of spent LIBs as solvent, we in situ fluorinate the conductive three-dimensional porous copper foam by a facile solvent-thermal method and then coating it with a cross-linked sodium alginate (SA) layer. Benefiting from the solid-electrolyte interphase (SEI) that accommodating the volume change of internal CuF2 core and SA layer that inhibiting the dissolution of CuF2, the synthesized CuF2@void@SEI@SA cathode with a pomegranate-like structure (yolk-shell) exhibits a large reversible capacity of ~535 mAh g-1 at 0.05 A g-1 and superb cycling stability. This work conforms to the development concept of green environmental protection and comprehensively realizes the unity of environmental, social and economic benefits.
Full text
Available for:
BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
BackgroundTo explore the impact of ARGs on the prognosis of NSCLC, and its correlation with clinicopathological parameters and immune microenvironment. Preliminary research on the biological ...functions of CEBPA in NSCLC.MethodsUsing consensus clustering analysis to identify molecular subtypes of ARGs in NSCLC patients; employing LASSO regression and multivariate Cox analysis to select 7 prognostic risk genes and construct a prognostic risk model; validating independent prognostic factors of NSCLC using forest plot analysis; analyzing immune microenvironment correlations using ESTIMATE and ssGSEA; assessing correlations between prognostic risk genes via qPCR and Western blot in NSCLC; measuring mRNA and protein expression levels of knocked down and overexpressed CEBPA in NSCLC using CCK-8 and EdU assays; evaluating the effects of knocked down and overexpressed CEBPA on cell proliferation using Transwell experiments; examining the correlation of CEBPA with T cells and B cells using mIHC analysis.ResultsConsensus clustering analysis identified three molecular subtypes, suggesting significant differential expression of these ARGs in NSCLC prognosis and clinical pathological parameters. There was significant differential expression between the two risk groups in the prognostic risk model, with P < 0.001. The risk score of the prognostic risk model was also P < 0.001. CEBPA exhibited higher mRNA and protein expression levels in NSCLC cell lines. Knockdown of CEBPA significantly reduced mRNA and protein expression levels of CEBPB, YWHAZ, ABL1, and CDK1 in H1650 and A549 cells. siRNA-mediated knockdown of CEBPA markedly inhibited proliferation, migration, and invasion of NSCLC cells, whereas overexpression of CEBPA showed the opposite trend. mIHC results indicated a significant increase in CD3 + CD4+, CD3 + CD8+, and CD20 + cell counts in the high CEBPA expression group.ConclusionsThe risk score of the prognostic risk model can serve as an independent prognostic factor, guiding the diagnosis and treatment of NSCLC. CEBPA may serve as a potential tumor biomarker and immune target, facilitating further exploration of the biological functions and immunological relevance in NSCLC.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Display omitted
•Arc synthesis of Ag@G with tailorable nanostructure and surface functionality.•Tailoring thickness enabled the synergy of π-π uptake and electron transfer for 4-NP.•Amino groups with ...proper densities induced the capture of Cr(VI) ultrafast.•Negative relation of shell thickness with formic acid-induced reduction of Cr(VI).•Reductive N heteroatoms with proper densities ensured a favorable reduction of Cr(VI).
Encapsulation of plasmonic nanoparticles in nanoshells is a promising strategy to overcome their oxidation-coalescence in the heterogeneous catalysis process. Tailoring of nanostructure/functionality to enable the efficient synergistic integration of core and shell is highly desired to enhance the plasmonic catalysis. This study applied a one-step arc discharge to design graphite-encapsulated silver (Ag@G) catalysts with tunable morphologies (14.3–46.9 nm of cores, 1.38–3.43 nm of shells) and surface functionalities (hydrophobization, or amination with maximum 2.70 × 1017/mg of Ag@G). Highly nanostructure/functionality-dependent catalysis of aquatic pollutants was established. Specifically, tailoring defective shells with thinner thickness enabled the efficient synergism of strong π-π coordination of 4-nitrophenol and effective electron transfer of donors. Aminated Ag@G with ultrathin shells presented an optimal reduction for Cr(VI), establishing a negative relation of thickness with formic acid-induced reduction. Amino with proper densities (9.6–20.9/nm2) induced the capture of Cr(VI) ultrafast. Moreover, reductive N heteroatoms with proper densities (e.g., 27.7 of N/nm2) provided abundant lone pair electrons without the dramatic decline of conductivity, ensuring a favorable reduction of Cr(VI). Since arc discharge was versatile for the industrial production of nanomaterials, our work would trigger a new upsurge to arc-design nanostructures/functionalities for the catalysis and decontamination.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Display omitted
•V-Ni@NiMoO4/Ni12P5 with a microsphere structure as the supporting framework exhibits excellent cycling stability.•For OER, a low overpotential of only 254 mV is required to achieve a ...current density of 50 mA cm, with no significant decline observed after 114 h of cycling.•The introduction of phosphorus enhances the electron transfer at the Ni active sites, better activating adsorbates, and thus improving catalytic performance.
At present, there are few reports on the micron-sized catalysts for overall water splitting. In this study, phosphating method were used to construct the self-supporting catalyst (V doped Ni microspheres coated by NiMoO4/Ni12P5) with microspherical structure, providing a short path and a stable structure to guarantee quick electron transfer and excellent catalytic performance. Hence, oxygen evolution reaction (OER) only needs 254 mV to reach a current density of 50 mA cm−2 in 1.0 mol/L KOH, after 114 h without attenuation. The catalyst can achieve a current densitiy of 10 mA cm−2 with a voltage of only 158 mV for hydrogen evolution reaction (HER). When micron scale V-Ni@NiMoO4/Ni12P5 is used as both anode and cathode for overall water splitting, the device can operate at a current density of 10 mA cm−2 for more than 200 h of good stability. Its superior catalytic performance can be attributed to the construction of micron size and phosphating. DFT calculations indicate that the introduction of P better activates the adsorbed *OH and H2O*, reduces reaction the energy barrier, and improves the catalytic activity.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
At present, there are few reports on the micron-sized catalysts for overall water splitting. In this study, phosphating method were used to construct the self-supporting catalyst (V doped Ni ...microspheres coated by NiMoO
/Ni
P
) with microspherical structure, providing a short path and a stable structure to guarantee quick electron transfer and excellent catalytic performance. Hence, oxygen evolution reaction (OER) only needs 254 mV to reach a current density of 50 mA cm
in 1.0 mol/L KOH, after 114 h without attenuation. The catalyst can achieve a current densitiy of 10 mA cm
with a voltage of only 158 mV for hydrogen evolution reaction (HER). When micron scale V-Ni@NiMoO
/Ni
P
is used as both anode and cathode for overall water splitting, the device can operate at a current density of 10 mA cm
for more than 200 h of good stability. Its superior catalytic performance can be attributed to the construction of micron size and phosphating. DFT calculations indicate that the introduction of P better activates the adsorbed *OH and H
O*, reduces reaction the energy barrier, and improves the catalytic activity.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
NiFe layered double hydroxide (LDH)-based materials arouse great attention because of their outstanding catalytic activity for oxygen evolution reaction (OER) under alkaline conditions. However, its ...catalytic activity for hydrogen evolution reaction (HER) under alkaline conditions is relatively poor. Herein, to improve the HER catalytic activity of NiFe LDH under alkaline conditions, we design a heterostructure of NiS and NiS2 on NiFe LDH and successfully achieve Mo element doping. The catalyst named NiFe-LDH@Mo–NiS–NiS2/NF possesses a three-dimensional nano-flower-like structure and shows an outstanding catalytic performance for both HER and OER. Specifically, in 1 M KOH solution, it requires the low overpotentials of 261 and 120 mV to achieve the current density of 50 and 10 mA cm−2 for OER and HER, respectively. Simultaneously, when NiFe-LDH@Mo–NiS2–NiS/NF is used as the bifunctional catalysts, it only needs a low voltage of 1.63 V to achieve a current density of 10 mA cm−2. The outstanding catalytic performance is attributed to its special heterostructure and the synergy between Mo and NiSx, resulting in comparability with most reported non-noble metal-based catalysts. The doping of metal elements and the construction of heterostructures provide new ideas for the structure regulation and performance improvement of bifunctional electrocatalysts.
Display omitted
•NiS2–NiS heterostructure with Mo doping three-dimensional nano-flower structure.•Large specific surface area and abundant active sites.•High oxygen evolution reaction and hydrogen evolution reaction catalytic and a low overpotential for overall water splitting.•Good cycle stability for 20 h test of oxygen evolution reaction and hydrogen evolution reaction tests.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Display omitted
•Electrocatalytic NB reduction is a mild and green route to obtain high-purity AN.•Composition control and morphology engineering viably upgrade the electrocatalyst.•CuNiAl with ...intermetallic compound displays conspicuous conversion and AN selectivity.•Nanoporous structure enlarges the specific surface and also facilitates the kinetics.•High efficiency for producing AN is achieved via inhibiting HER at low overpotential.
Electrocatalytic nitrobenzene (NB) hydrogenation with high selectivity, yield and Faradaic efficiency (FE) is an economic and sustainable approach to produce high-purity aniline (BA). However, its implementation is essentially hindered by state-of-the-art Cu-based metallic electrocatalysts, which are of insufficient intrinsic activity and unstable nanostructures, in addition to low FE caused by irrepressibly competitive hydrogen evolution reaction at high overpotential. Herein, we report a self-supported nanoporous CuNiAl alloy as a highly efficient electrocatalyst towards NB hydrogenation reaction. Owing to the incorporation of Ni in intermetallic Al4Cu9(Ni), which is of high intrinsic activity, and the hierarchical nanoporous architecture, which facilitates electron transfer and mass transportation along interconnective metal ligaments and interpenetrative pore channels, self-supported nanoporous CuNiAl electrode exhibits remarkably enhanced NB hydrogenation electrocatalysis for BA product with high selectivity (98%), yield (88.5%) and FE (82.3%) at ultralow operating potential (−0.07 V versus RHE). This nanoporous CuNiAl alloy outperforms some of the best Cu-based nanocatalysts reported previously. This work makes a stride for designing novel low-cost electrocatalysts for high-efficient NB hydrogenation by following composition control and morphology engineering.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Urine-derived stem cells (USCs) are isolated from voided urine and display high proliferative activity and multiple differentiation potentials. The applicability of USCs in the treatment of bladder ...dysfunction and in cell-based urological tissue engineering has been demonstrated. Whether they could serve as a potential stem cell source for the treatment of diabetes mellitus (DM) and its complications has not been investigated. Here, we report the repairing and protective effects of USCs on pancreatic islets, the myocardium, the renal glomerulus and the bladder detrusor in diabetic rat models. Type 2 diabetic rat models were induced by means of a high fat diet and intraperitoneal injection with streptozotocin. USCs isolated from voided urine were administered via tail veins. The functional changes of pancreatic islets, left ventricle, glomerulus and bladder micturition were assessed by means of insulin tolerance tests, echocardiography, urine biochemical indexes and cystometry. The histologic changes were evaluated by hematoxylin and eosin staining, Masson's trichrome staining and TUNEL staining. Treatment with USCs significantly alleviated the histological destruction and functional decline. Although the USC treatment did not decrease fasting blood glucose to a significantly different level, the fibrosis and apoptosis of the myocardium, glomerulus and detrusor were significantly inhibited. This study indicates that administration of USCs may be useful for the treatment of the complications of DM.
•This is the first study to investigate the therapeutic effects of USCs on T2D rats. .•Injected USCs can differentiate into PDX1-positive cells in rat pancreas.•Ameliorating the fibrosis and inhibiting cell apoptosis is the possible pathway for the treatment of complications.•USCs are good resource for the treatment of diabetes mellitus and diabetic complications.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
This article presents the generation of monoclonal antibodies (mAbs) with high specificity against 19-nortestosterone (NT) through cell fusion techniques and the development of a mAb-based indirect ...competitive ELISA (icELISA) method and colloidal gold-based immuno-chromatographic assay to detect NT residues in beef and pork samples. A modified carbodiimide method was employed to synthesize the artificial antigen, and BALB/c mice were used to produce anti-NT mAbs. On the basis of the checkerboard titration, an indirect competitive ELISA standard curve was established. This assay was sensitive and had a linear range from 0.03 to 38 ng/mL in phosphate buffered saline (PBS), with IC50 and LOD values of 0.52 ng/mL and 0.01 ng/mL, respectively. Of all the competitive analogues, the produced mAb exhibited a high cross-reactivity to 17α-nortestosterone (83.6%), the main metabolite of NT in animal tissues. Except for moderate cross-reactivities with trenbolone (22.6%) and β-boldenone (13.8%), the other interference to the assay was negligible (<0.05%). In contrast, the strip test had a visual detection limit of 1 ng/mL in PBS, 2 μg/kg in beef, and 2 μg/kg in pork, respectively, and the results can be judged within 10 min. The ELISA and GC-MS results showed close correlation in beef (R 2 = 0.9945) and in pork (R 2 = 0.9977). Therefore, the combination of two immunoassays provides a useful screening method for quantitative or qualitative detection of NT residues in animal-origin products.
Full text
Available for:
IJS, KILJ, NUK, PNG, UL, UM, UPUK
Human urine-derived stem cells (hUSCs) show multipotential differentiation ability and can differentiate into mesodermal cell lineages. Interstitial cells of Cajal-like cells (ICC-LCs) are crucial ...for the pace-making function of spontaneous contraction in the bladder. However, the mechanisms by which hUSCs generate ICC-LCs have not been elucidated. In this study, we developed a strategy for directional differentiation of hUSCs into ICC-LCs. hUSCs were transfected with lentiviral vectors encoding c-Kit, stem cell factor (SCF), hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), and 5-azacytidine induced 2 (AZI2) genes, and the cells were cultured for an additional 7 days in specific medium. The expression of the surface marker c-Kit on ICC-LCs was determined at 7 days after transfection. hUSCs were successfully expanded and transfected with the four lentiviral vectors. hUSCs transfected with lentiviral-c-Kit, lentiviral-HCN4, and lentiviral-AZI2 showed higher expression of c-Kit 7 days after transfection, but only the lentiviral-HCN4-transfected cells showed morphological alterations in ICC-LCs. These cells also displayed visible HCN current amplitude and density. This approach may provide a new strategy for the treatment of underactive bladder.
•HCN4 overexpression altered hUSCs morphology to that of ICC-LCs.•HCN4 overexpression induced functional HCN currents of ICC-LCs in hUSCs.•HCN4 overexpression produced a preliminary ICC-LC-like phenotype of hUSCs.•We present novel strategies for the treatment of underactive bladder diseases.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
PDF
