Fingerprint-based localization relies on an accurate and up-to-date radio map, which is however cumbersome to obtain. In this paper, a novel scheme is proposed to online adapt radio maps to ...environmental dynamics by using low-cost crowdsourced received signal strength (RSS) measurements. To be specific, a coarse-grained radio map is initially established in the offline phase utilizing the standard Gaussian process regression (GPR) given a limited number of fingerprints (i.e., RSS measurements with location labels), and further can be recursively refined in the online phase given crowdsourced RSS measurements with their noisy location labels obtained through the existing radio map. Differently from existing GPR-based approaches, the proposed scheme adopts extended GPR to alleviate the model inaccuracy induced by such noisy location labels, and then presents a marginalized particle extended Gaussian process (MPEG) to recursively filter the radio map. In addition, pedestrian dead reckoning (PDR) is leveraged to calibrate such noisy location labels. Extensive experiments are carried out in a real scenario with area of nearly 1000 m 2 during a five-month period of time, and a thorough comparison with several existing approaches indicates that the proposed scheme gradually improves the localization accuracy on average by as much as 31.2%, while the counterparts result in fluctuant localization performance and improve the localization accuracy on average by 13.3%.
In utilizing glycerol to produce 1,3‐propanediol by microbial fermentation, the problems of low utilization rate and poor production performance need to be addressed. Based on the analysis of a ...mathematical model for 1,3‐propanediol production from glycerol by Klebsiella pneumoniae, this study theoretically investigated the effects of the dilution rate and the initial glycerol concentration in a two‐stage fermentation process and the feasibility of applying the feedback control methods. First, the optimal operation conditions of initial glycerol concentration and dilution rate were obtained. Through the use of feedback control theory, a control strategy for dilution rate was designed and optimized to shorten the settling time (time required for fermentation to reach stability) from 60.92 to 36.68 h for the first reactor, and from 53.66 to 22.68 h for the second reactor. In addition, the yield of 1,3‐propanediol in both two reactors reached up to 0.5 g·g−1. The simulation results indicated that the feedback control strategy for dilution rate increased the product concentration, reduced the residual glycerol in the fermentation broth, and greatly improved the performance of the fermentation. A feeding strategy of automatic control for dilution rate has been established and will be applied as an effective guiding scheme in automatic continuous fermentations for production of 1,3‐propanediol.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Parathyroid hormone (1–34, PTH) combined β-tricalcium phosphate (β-TCP) achieves stable bone regeneration without cell transplantation in previous studies. Recently, with the development of tissue ...engineering slow release technology, PTH used locally to promote bone defect healing become possible. This study by virtue of collagen with a combination of drugs and has a slow release properties, and investigated bone regeneration by β-TCP/collagen (β-TCP/COL) with the single local administration of PTH. After the creation of a rodent critical-sized femoral metaphyseal bone defect, β-TCP/COL was prepared by mixing sieved granules of β-TCP and atelocollagen for medical use, then β-TCP/COL with dripped PTH solution (1.0 µg) was implanted into the defect of OVX rats until death at 4 and 8 weeks. The defected area in distal femurs of rats was harvested for evaluation by histology, micro-CT, and biomechanics. The results of our study show that single-dose local administration of PTH combined local usage of β-TCP/COL can increase the healing of defects in OVX rats. Furthermore, treatments with single-dose local administration of PTH and β-TCP/COL showed a stronger effect on accelerating the local bone formation than β-TCP/COL used alone. The results from our study demonstrate that combination of single-dose local administration of PTH and β-TCP/COL had an additive effect on local bone formation in osteoporosis rats.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
ABSTRACTMl muscarinic acetylcholine receptors are highly expressed in key areas that control cognition, such as the cortex and hippocampus, representing one potential therapeutic target for cognitive ...dysfunctions of Alzheimer's disease and schizophrenia. We have reported that M1 receptors facilitate cognition by promoting membrane insertion of α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid receptor AMPA receptor subunit 1 (GluA1) through phosphorylation at Ser845. However, the signaling pathway is still unclear. Here we showed that adenylyl cyclase inhibitor 2′,5′‐dideoxyadenosine and PKA inhibitor KT5720 inhibited enhancement of phosphorylation of Ser845 and membrane insertion of GluA1 induced by M1 receptor activation. Furthermore, PI3K inhibitor LY294002 and protein kinase B (Akt) inhibitor IV blocked the effects of M1 receptors as well. Remarkably, the increase of the activity of PI3K‐Akt signaling induced by M1 receptor activation could be abolished by cAMP‐PKA inhibitors. Moreover, inhibiting the mammalian target of rapamycin (mTOR) complex 1, an important downstream effector of PI3K‐Akt, by short‐term application of rapamycin attenuated the effects of Ml receptors on GluA1. Furthermore, such effect was unrelated to possible protein synthesis promoted by mTOR. Taken together, these data demonstrate that M1 receptor activation induces membrane insertion of GluA1 via a signaling linking cAMP‐PKA and PI3K‐Akt‐mTOR pathways but is irrelevant to protein synthesis.—Zhao, L.‐X., Ge, Y.‐H., Li, J.‐B., Xiong, C.‐H., Law, P.‐Y., Xu, J.‐R., Qiu, Y., Chen, H.‐Z. M1 muscarinic receptors regulate the phosphorylation of AMPA receptor subunit GluA1 via a signaling pathway linking cAMP‐PKA and PI3K‐Akt. FASEB J. 33, 6622–6631 (2019). www.fasebj.org
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The present study was conducted to evaluate the influences of five dietary manganese (Mn) sources on growth, feed utilization, lipid metabolism, antioxidant capacity, inflammatory response and ...endoplasmic reticulum stress in intestinal tissues of yellow catfish Pelteobagrus fulvidraco. Five Mn sources include Mn sulfate monohydrate (MnSO4·H2O), Mn dioxide (MnO2), Mn dioxide nanoparticles (MnO2NPs), Mn glycine chelate (Mn-Gly) and hydroxymethionine-chelated Mn (Mn-MHA). Yellow catfish (initial body weight: 2.69 ± 0.01 g/fish) were fed five experimental diets for 10 weeks. Dietary Mn sources significantly affected intestinal Mn content and metabolism. Compared to other Mn sources, yellow catfish fed organic Mn sources (Mn-Gly and Mn-MHA) tended to improve growth, feed utilization, intestinal histology, up-regulated mRNA abundance of tight junction-relevant genes, increased intestinal fatty acid uptake and transport and triglyceride deposition, and reduced inflammatory responses. These dietary organic Mn supplements also increased intestinal antioxidant capacity by up-regulating antioxidant gene expression and enzymatic activities. Yellow catfish fed two organic Mn diets had the lower mRNA expressions of endoplasmic reticulum stress-relevant gene and protein expression. Based on these observations above, dietary organic supplements (Mn-Gly and Mn-MHA) improved the growth and feed utilization, and promoted intestinal health of yellow catfish, and accordingly seems to be optimal Mn sources for yellow catfish.
Our results provided the innovative insights into dietary Mn sources influencing tight junction, antioxidant capacity and inflammatory responses, endoplasmic reticulum stress and fatty acid uptake and uptake in the fish intestine, and found that organic Mn sources were more suitable as the Mn supplements in yellow catfish feeds, and also possibly in other aquatic feed.
•Two organic Mn sources in yellow catfish diet positively affect growth performance.•Two organic Mn sources in yellow catfish diet improved intestinal tight junction.•Two organic Mn in yellow catfish diet increased fatty acid uptake and TG deposition.•Two organic Mn reduced intestinal inflammation and increased antioxidant capacity.•Two organic Mn sources in yellow catfish diet improved the ER function.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Nonalcoholic fatty liver disease (NAFLD) is considered as a liver manifestation of metabolic disorders. Previous studies indicate that the renin-angiotensin system (RAS) plays a complex role in ...NAFLD. As the only precursor of the RAS, decreased angiotensinogen (AGT) profoundly impacts RAS bioactivity. Here, we investigated the role of hepatocyte-derived AGT in liver steatosis. AGT floxed mice (hepAGT+/+) and hepatocyte-specific AGT-deficient mice (hepAGT−/−) were fed a Western diet and a normal laboratory diet for 12 weeks, respectively. Compared with hepAGT+/+ mice, Western diet-fed hepAGT−/− mice gained less body weight with improved insulin sensitivity. The attenuated severity of liver steatosis in hepAGT−/− mice was evidenced by histologic changes and reduced intrahepatic triglycerides. The abundance of SREBP1 and its downstream molecules, acetyl-CoA carboxylase and FASN, was suppressed in hepAGT−/− mice. Furthermore, serum derived from hepAGT+/+ mice stimulated hepatocyte SREBP1 expression, which could be diminished by protein kinase B (Akt)/mammalian target of rapamycin (mTOR) inhibition in vitro. Administration of losartan did not affect diet-induced body weight gain, liver steatosis severity, and hepatic p-Akt, p-mTOR, and SREBP1 protein abundance in hepAGT+/+ mice. These data suggest that attenuation of Western diet-induced liver steatosis in hepAGT−/− mice is associated with the alternation of the Akt/mTOR/SREBP-1c pathway.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
A polymer （poly（9,10）anthracenevinylene-alt-4,4＇-（9,9-bis（4-（4＇-（1,2,2＇-triphenyviny）phenoxy）butyl）-9H- fluorene-2,7-diyl） dibenzaldehyde）, P1） was successfully synthesized through the Wittig-Horner ...reaction by employing fluorene and 9,10-distyrylanthracene moieties as building blocks for backbone and tetraphenylethenes as pendant groups. Photophysical and thermal properties of the resulting polymeric emitter were fully characterized by ultraviolet-visible （UV- Vis） absorption and photoluminescence （PL） spectra, thermogravimetric analysis （TGA） and differential scanning calorimetry （DSC）. While P1 emits an orange-light centered at 567 nm in dilute tetrahydrofuran （THF） solution, the solid powder of the polymer exhibits strong yellow emission peaked at 541 nm. It is also found that the as-synthesized polymer shows unique property of aggregation-enhanced emission （AEE）. In addition, P1 possesses high thermal stability with a decomposition temperature （Td,5%） of 430 ℃ and high morphological stability with a glass transition temperature （Tg） of 171℃. Under the stimulus of mechanical force, the emission of P1 can be changed from yellow to red （△λmax = 61 rim）, showing a remarkable mechanochromism. The results from XRD analysis suggest that such mechanochromic phenomenonof PI is probably caused by the destruction of crystalline structure, which leads to the conformational planarization of the distyrylanthracene moieties forming by the polymerization and the increase of molecular conjugation of the backbone.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Forest bathing trip is a short, leisurely visit to forest. In this study we determined the health effects of forest bathing trip on elderly patients with chronic obstructive pulmonary disease （COPD）. ...The patients were randomly divided into two groups. One group was sent to forest, and the other was sent to an urban area as control. Flow cytometry, ELISA, and profile of mood states （POMS） evaluation were performed. In the forest group,
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Via implementing parallel tasks including Santa-Fe time series prediction and nonlinear channel equalization, we studied the characteristics of reservoir computing (RC) using two mutually-coupled ...semiconductor lasers (MC-SLs) with optoelectronic feedback. The influence of system parameters (injection current, coupling strength, scaling factor and coupling time-delays) on RC performance have been investigated in detail. Results show that, the optimized performance is always achieved around the edge of bifurcation points; positive feedback is more preferred when the scaling factor exceeds a certain level; under optimized parameters, both of lower normalized mean squared error (NMSE) and symbol error rate (SER) can be achieved to perform parallel tasks, due to the richer dynamics and states of virtual nodes. The effect of system parameters on RC performance was also discussed. Our study would be helpful for the design and optimization of proposed parallel RC with optoelectronic feedback.
•Reservoir computing by mutually-coupled lasers with optoelectronic feedback was proposed.•The influence of system parameters on RC performance was investigated comprehensively.•The proposed structure could provide a new version for parallel reservoir computing.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
In the context of the coronavirus disease 2019 pandemic, we investigated the epidemiological and clinical characteristics of a young patient infected by avian influenza A (H5N6) virus in Anhui ...Province, East China, and analyzed genomic features of the pathogen in 2020. Through the cross‐sectional investigation of external environment monitoring (December 29–31, 2020), 1909 samples were collected from Fuyang City. It was found that the positive rate of H5N6 was higher than other areas obviously in Tianma poultry market, where the case appeared. In addition, dual coinfections were detected with a 0.057% polymerase chain reaction positive rate the surveillance years. The virus was the clade 2.3.4.4, which was most likely formed by genetic reassortment between H5N6 and H9N2 viruses. This study found that the evolution rates of the hemagglutinin and neuraminidase genes of the virus were higher than those of common seasonal influenza viruses. The virus was still highly pathogenic to poultry and had a preference for avian receptor binding.
Highlights
Various avian influenza virus (AIV) subtypes naturally have caused zoonotic infections, but the subtypes H5N1 and H7N9 have caused a prominent impact.
At present, the outbreak of novel coronavirus pneumonia (coronavirus disease 2019 COVID‐19) has caused a worldwide pandemic.
To our knowledge, this is the youngest child infected with H5N6 subtype avian influenza in Anhui Province.
Herein, we analyzed the epidemiology of the case and the characteristics of the pathogen genome, to find out the possible evolution of the virus.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
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