The relationship between cancer and microorganisms has been extensively studied, with bacteria receiving more attention than fungi. However, fungi have been shown to play a significant role in cancer ...development and progression. Understanding the underlying mechanisms is crucial for identifying new avenues in prevention and treatment. To evaluate the current state of research on fungi and cancer, we conducted a comprehensive bibliometric analysis. Using the Web of Science Core Collection database, we searched for English-language articles published between 1998 and 2022. Analyzing the resulting publication data, we identified trends, patterns, and research gaps. Our analysis encompassed co-authorship networks, citation analysis, and keyword co-occurrence analysis. With 8283 publications identified, averaging 331.32 publications per year, our findings highlight China, the United States, India, Japan, and Germany as the top contributing countries. The Chinese Academy of Sciences, Sun Yat-Sen University, and University of São Paulo emerged as the most productive institutions. Key themes in the literature included “cancer,” “cytotoxicity,” “apoptosis,” “metabolites,” and “fungus.” Recent trends indicate increased interest in keywords such as “green synthesis,” “molecular docking,” “anticancer activity,” “antibacterial,” “anticancer,” and “silver nanoparticles.” Our study provides a comprehensive assessment of the current research landscape in the field of fungi and cancer, offering insights into collaborative networks, research directions, and emerging hotspots. The growing publication rate demonstrates the rising interest in the topic, while identifying leading countries, institutions, and research themes serves as a valuable resource for researchers, policymakers, and funders interested in supporting investigations on fungi-derived compounds as potential anti-cancer agents.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Glutamate carboxypeptidase II (GCPII) is a transmembrane zinc metallopeptidase found mainly in the nervous system, prostate and small intestine. In the nervous system, glia‐bound GCPII mediates the ...hydrolysis of the neurotransmitter N‐acetylaspartylglutamate (NAAG) into glutamate and N‐acetylaspartate. Inhibition of GCPII has been shown to attenuate excitotoxicity associated with enhanced glutamate transmission under pathological conditions. However, different strains of mice lacking the GCPII gene are reported to exhibit striking phenotypic differences. In this study, a GCPII gene knockout (KO) strategy involved removing exons 3–5 of GCPII. This generated a new GCPII KO mice line with no overt differences in standard neurological behavior compared to their wild‐type (WT) littermates. However, GCPII KO mice were significantly less susceptible to moderate traumatic brain injury (TBI). GCPII gene KO significantly lessened neuronal degeneration and astrocyte damage in the CA2 and CA3 regions of the hippocampus 24 h after moderate TBI. In addition, GCPII gene KO reduced TBI‐induced deficits in long‐term spatial learning/memory tested in the Morris water maze and motor balance tested via beam walking. Knockout of the GCPII gene is not embryonic lethal and affords histopathological protection with improved long‐term behavioral outcomes after TBI, a result that further validates GCPII as a target for drug development consistent with results from studies using GCPII peptidase inhibitors.
The peptide neurotransmitter N‐acetylaspartylglutamate (NAAG) suppresses glutamate transmission through selective activation of pre‐synaptic Group II metabotropic glutamate receptor subtype 3 (mGluR3) after traumatic brain injury (TBI). However, synaptically released NAAG is hydrolyzed to form N‐acetylaspartate and glutamate mainly by Glutamate carboxypeptidase II (GCPII), losing neuroprotective effect. In this study, we found that knock out of the GCPII gene is not embryonic lethal and affords histopathological protection with improved long‐term behavioral outcomes after TBI.
The peptide neurotransmitter N‐acetylaspartylglutamate (NAAG) suppresses glutamate transmission through selective activation of pre‐synaptic Group II metabotropic glutamate receptor subtype 3 (mGluR3) after traumatic brain injury (TBI). However, synaptically released NAAG is hydrolyzed to form N‐acetylaspartate and glutamate mainly by Glutamate carboxypeptidase II (GCPII), losing neuroprotective effect. In this study, we found that knock out of the GCPII gene is not embryonic lethal and affords histopathological protection with improved long‐term behavioral outcomes after TBI.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
A heterogeneous Cu(II)-AOFs catalyst, Cu(II) supported on amidoxime fibers (AOFs), is successfully applied to the cross-dehydrogenative coupling of aromatic terminal alkynes and N,N-dimethylanilines ...to form propargylamines. The Cu(II)-AOFs catalyst shows high catalytic activity with the yields of the corresponding propargylamines reaching 90% at 70 °C for 4 h without the protection of an inert gas. The Cu(II)-AOFs coordinate with the imine ion intermediate generated during the reaction, making the coupling facile. X-ray photoelectron spectrometer, scanning electron microscope, and energy dispersive X-ray spectroscopy results show that Cu(II) successfully coordinates with N and O atoms on the surface of the fibers with the mutual conversion between monovalent and divalent Cu in the Cu-AOFs being the catalytically active center. The catalyst can be recycled more than four times, and the catalytic activity is not obviously reduced. This process represents a new pathway for the formation of propargylamines using a Cu(II)-AOFs catalyst.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Background
DNA damage response (DDR) proficiency is the principal mechanism of temozolomide (TMZ) resistance in glioma. Accumulating evidence has also suggested the determining role of DDR in ...anticancer immunity. We propose that a comprehensive investigation of the DDR landscape can optimize glioma treatment.
Methods
We identified the pronounced enrichment of DDR in TMZ-resistant glioma cells by RNA sequencing. Nine differentially expressed genes between TMZ-sensitive/resistant glioma cells were selected to construct the DDR score through lasso regression analysis. Two glioma cohorts from TCGA and CGGA were interrogated to evaluate the predictive ability of DDR score. Multiple algorithms were applied to estimate the immunotherapeutic responses of two DDR phenotypes. Immunohistochemistry was used to determine the protein levels of PD-L1 and TGFβ in glioma specimens. The oncoPredict package was employed to predict the candidate chemotherapy agents.
Results
DDR score exhibited a robust prognostic capability in TCGA and CGGA cohorts and served as an independent predictive biomarker in glioma patients. Functional enrichment analyses revealed that high and low DDR score groups were characterized by distinct immune activity and metabolic processes. Elevated levels of infiltrating immune cells (including CD8+ T cells, CD4+ T cells, and dendritic cells) were observed in the high DDR score glioma. Further, high DDR scores correlated with increased mutation burden, up-regulated immune checkpoints, and tumor immunity activation, indicating a profound interplay between DDR score and glioma immunogenicity. In addition, PD-L1 and TGFβ were overexpressed in recurrent glioma specimens compared with primary ones. Finally, we estimated that PI3K inhibitors may serve as latent regimens for high DDR score patients.
Conclusion
Our study highlighted the promising prognostic role of DDR score in glioma. Individual assessment of DDR status for patients with glioma may provide new clues for developing immunotherapeutic strategies.
Long noncoding RNA (lncRNA) has played the important function in regulation of various biological processes and in diagnostic value has been widely appreciated. In the present study, we have found ...that LOC101928316 was significantly downregulated in gastric cancer (GC) tissues specimen, GC cell lines, and associated with the GC patients tumor, node, and metastasis (TNM) stage and degree of differentiation (P ＜ 0.05). LOC101928316 overexpression can significantly inhibit SGC‐7901 cell migration, invasion, and proliferation (P＜0.05). LOC101928316 molecular mechanism investigates suggested that LOC101928316 can regulate PI3K‐Akt‐mTOR signaling pathway and change the GC development progression in vivo and in vitro. In vivo experiment also revealed that LOC101928316‐Overexpression can inhibit the tumorigenicity of GC cells in tumor‐burdened experimental nude mice (P ＜ 0.05). LOC101928316 may function as anti‐oncogene and also plays an important role in GC tumorigenesis. Collectively, our data provided the key role of LOC101928316 in the tumorigenesis of GC. In addition, the present study elucidates LOC101928316 potential regulatory network, which may help us to lead a better knowing of the pathogenesis of GC and probe the lncRNA as a novel biomarker to diagnosis and therapy for this malignant tumor.
Study systematically investigates the relationship and potential regulation mechanism between LOC101928316 and gastric cancer. Results suggested that gastric cancer tissues downregulated lncRNA LOC101928316 may be potential biomarker for diagnosis and prognosis of gastric cancer, which is an interesting indicator for further research.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Observations of debris disks, the products of the collisional evolution of rocky planetesimals, can be used to trace planetary activity across a wide range of stellar types. The most common end ...points of stellar evolution are no exception, as debris disks have been observed around several dozen white dwarf stars. But instead of planetary formation, post-main-sequence debris disks are a signpost of planetary destruction, resulting in compact debris disks from the tidal disruption of remnant planetesimals. In this work, we present the discovery of five new debris disks around white dwarf stars with gaseous debris in emission. All five systems exhibit excess infrared radiation from dusty debris, emission lines from gaseous debris, and atmospheric absorption features indicating ongoing accretion of metal-rich debris. In four of the systems, we detect multiple metal species in emission, some of which occur at strengths and transitions previously unseen in debris disks around white dwarf stars. Our first year of spectroscopic follow-up hints at strong variability in the emission lines that can be studied in the future, expanding the range of phenomena these post-main-sequence debris disks exhibit.
Increasing evidence indicates that interferon alpha (IFN-α) therapy is an effective treatment option for a subgroup of patients with chronic hepatitis B virus (HBV) infection. It has been confirmed ...that interferon-induced protein with tetratricopeptide repeats 3 (IFIT3), a member of the interferon-stimulated genes (ISGs), could inhibit the replication of various viruses. However, its effect on HBV replication is unclear. The present study sought to explore the role and mechanism of IFIT3 in IFN-α antiviral activities against HBV. IFIT3 mRNA levels in the peripheral blood of 108 treatment-naive patients and 70 healthy controls were analyzed first. The effect of IFIT3 on the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway under the dual intervention of IFN-α and HBV was also explored
. Treatment-naive individuals exhibited elevated levels of IFIT3 mRNA compared to the controls (
< 0.0001). Mechanistically, the knockdown of IFIT3 inhibited the phosphorylation of signal transducer and activator of transcription 2 (STAT2), whereas the overexpression of IFIT3 produced the opposite effect
. Meanwhile, the overexpression of IFIT3 enhanced the expression of IFN-α-triggered ISGs, including myxovirus resistance A (MxA), 2'-5'-oligoadenylate synthetase 1 (OAS1), and double-stranded RNA-activated protein kinase (PKR), while a weaker induction of IFN-α-triggered ISGs was observed in ruxolitinib-treated cells. After decreasing IFIT3 expression by validated small hairpin RNAs (shRNAs), the levels of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and HBV DNA secreted by HepG2 cells transiently transfected with the pHBV1.2 plasmid were increased. Our findings suggest that IFIT3 works in a STAT2-dependent manner to promote the antiviral effect of IFN-α through the JAK-STAT pathway in HBV infection in both human hepatocytes and hepatocarcinoma cells.
Our study contributes new insights into the understanding of the functions and roles of interferon-induced protein with tetratricopeptide repeats 3 (IFIT3), which is one of the interferon-stimulated genes induced by hepatitis B virus infection in human hepatocytes and hepatocarcinoma cells, and may help to identify targeted genes promoting the efficacy of interferon alpha.
Abstract
Brown dwarfs in close-in orbits around white dwarfs offer an excellent opportunity to investigate properties of fast-rotating, tidally locked, and highly irradiated atmospheres. We present ...Hubble Space Telescope Wide Field Camera 3 G141 phase-resolved observations of two brown-dwarf-white-dwarf binaries: WD 0137-349 and EPIC 212235321. Their 1.1–1.7
μ
m phase curves demonstrate rotational modulations with semi-amplitudes of 5.27% ± 0.02% and 29.1% ± 0.1%; both can be fit well by multi-order Fourier series models. The high-order Fourier components have the same phase as the first-order and are likely caused by hot spots located at the substellar points, suggesting inefficient day/night heat transfer. Both brown dwarfs’ phase-resolved spectra can be accurately represented by linear combinations of their respective day- and nightside spectra. Fitting the irradiated brown dwarf model grids to the dayside spectra require a filling factor of ∼50%, further supporting a hot spot dominating the dayside emission. The nightside spectrum of WD 0137-349B is fit reasonably well by non-irradiated substellar models, and the one of EPIC 21223521B can be approximated by a Planck function. We find strong spectral variations in the brown dwarfs’ day/night flux and brightness temperature contrasts, highlighting the limitations of band-integrated measurements in probing heat transfer in irradiated objects. On the color–magnitude diagram, WD 0137-349B evolves along a cloudless model track connecting the early-L and mid-T spectral types, suggesting that clouds and disequilibrium chemistry have a negligible effect on this object. A full interpretation of these high-quality phase-resolved spectra calls for new models that couple atmospheric circulation and radiative transfer under high-irradiation conditions.
ABSTRACT
Planets and stars ultimately form out of the collapse of the same cloud of gas. Whilst planets, and planetary bodies, readily loose volatiles, a common hypothesis is that they retain the ...same refractory composition as their host star. This is true within the Solar system. The refractory composition of chondritic meteorites, Earth, and other rocky planetary bodies are consistent with solar, within the observational errors. This work aims to investigate whether this hypothesis holds for exoplanetary systems. If true, the internal structure of observed rocky exoplanets can be better constrained using their host star abundances. In this paper, we analyse the abundances of the K-dwarf, G200-40, and compare them to its polluted white dwarf companion, WD 1425+540. The white dwarf has accreted planetary material, most probably a Kuiper belt-like object, from an outer planetary system surviving the star’s evolution to the white dwarf phase. Given that binary pairs are chemically homogeneous, we use the binary companion, G200-40, as a proxy for the composition of the progenitor to WD 1425+540. We show that the elemental abundances of the companion star and the planetary material accreted by WD 1425+540 are consistent with the hypothesis that planet and host-stars have the same true abundances, taking into account the observational errors.
Cervical cancer (CC) is a common gynecological malignancy in women worldwide. Evidence suggests that long non-coding RNAs (lncRNAs) can be used as biomarkers in patients with CC. However, prognostic ...biomarkers for CC are still lacking. The aim of our study was to find lncRNA biomarkers which are able to predict prognosis in CC based on the data from The Cancer Genome Atlas (TCGA).
The patients were divided into three groups according to FIGO stage. Differentially expressed lncRNAs were identified in CC tissue compared to adjacent normal tissues based on a fold change >2 and <0.5 at
< 0.05 for up- and downregulated lncRNA, respectively. The relationship between survival outcome and lncRNA expression was assessed with univariate and multivariate Cox proportional hazards regression analysis. We constructed a risk score as a method to evaluate prognosis. We used receiver operating characteristic (ROC) curve and the area under curve (AUC) analyses to assess the diagnostic value of a two-lncRNA signature. We detected the expression levels of the two lncRNAs in 31 pairs of newly diagnosed CC specimens and paired adjacent non-cancerous tissue specimens, and also in CC cell lines. Finally, the results were statistically compared using
-tests.
In total, 289 RNA sequencing profiles and accompanying clinical data were obtained. We identified 49 differentially expressed lncRNAs, of which two related to overall survival (OS) in CC patients. These two lncRNAs (ILF3-AS1 and RASA4CP) were found together as a single prognostic signature. Meanwhile, the prognosis of patients with low-risk CC was better and positively correlated with OS (
< 0.001). Further analysis showed that the combined two-lncRNA expression signature could be used as an independent biomarker to evaluate the prognosis in CC. qRT-PCR results were consistent with TCGA, confirming downregulated expression of both lncRNAs. Furthermore, upon ROC curve analysis, the AUC of the combined lncRNAs was greater than that of the single lncRNAs alone (0.723 vs 0.704 and 0.685), respectively;
< 0.05.
Our study showed that the two-lncRNA signature of ILF3-AS1 and RASA4CP can be used as an independent biomarker for the prognosis of CC, based on bioinformatic analysis.
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