Gastric cancer remains a significant health burden worldwide. In continuation of our previous study and development of effective small molecules against gastric cancer, a series of benzochalcone ...analogues involving heterocyclic molecules were synthesised and biologically evaluated in vitro and in vivo. Among them, the quinolin-6-yl substituted derivative KL-6 inhibited the growth of gastric cancer cells (HGC27, MKN28, AZ521, AGS, and MKN1) with a submicromolar to micromolar range of IC
50
, being the most potent one in this series. Additionally, KL-6 significantly inhibited the colony formation, migration and invasion, and effectively induced apoptosis of MKN1 cells in a concentration-dependent manner. The mechanistic study revealed that KL-6 could concentration-dependently suppress STAT3 phosphorylation, which may partly contribute to its anticancer activity. Furthermore, in vivo antitumour study on the MKN1 orthotopic tumour model showed that KL-6 effectively inhibited tumour growth (TGI of 78%) and metastasis without obvious toxicity. Collectively, compound KL-6 may support the further development of candidates for gastric cancer treatment.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Nitrite-dependent anaerobic methane oxidation (N-DAMO) is a recently discovered process linking the global carbon and nitrogen cycles. This process was reported to be mediated by “
Candidatus ...Methylomirabilis oxyfera
”. To date,
M. oxyfera
-like bacteria have been detected in a limited number of freshwater habitats, but whether these bacteria occur in estuarine habitats is currently unknown. In this study, the distribution, diversity and abundance of
M. oxyfera
-like bacteria were studied in the sediment of the Jiaojiang Estuary of the East Sea (China). Both the 16S ribosomal RNA (rRNA) and
pmoA
genes confirmed the occurrence of
M. oxyfera
-like bacteria in the examined estuary. The recovered 16S rRNA gene sequences showed 91.5–97.2 % identity to the 16S rRNA gene of
M. oxyfera
, and the recovered
pmoA
gene sequences showed 85.1–95.4 % identity to the
pmoA
gene of
M. oxyfera
. Quantitative PCR further confirmed the occurrence of
M. oxyfera
-like bacteria in this estuary, with the abundance varying from 5.80 ± 0.28 × 10
4
to 8.35 ± 0.52 × 10
7
copies g (dry weight)
−1
. Correlation analysis indicated that the sediment organic content was the most important factor affecting the distribution of
M. oxyfera
-like bacterial communities in the examined sediments among the environmental factors investigated. This study demonstrated for the first time the existence of
M. oxyfera
-like bacteria in an estuarine environment and showed the correlations between the distribution of these bacteria and the estuarine environmental conditions.
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CEKLJ, DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NUK, OBVAL, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Diabetes is a chronic metabolic disease characterized by elevated blood glucose levels due to insulin resistance and β-cell dysfunction. This study aims to examine the effects of polysaccharides from ...adlay seeds (PAS) on hyperglycemia and gut microbiota in streptozocin (STZ)-induced diabetic mice. The administration of PAS in diabetic mice caused a significant decrease in the glucose level and serum levels of glycosylated hemoglobin (HbA1c). Similarly, PAS also showed decreased total cholesterol (TC) and triglyceride (TG) concentrations. Furthermore, a significant increase in the concentrations of glucagon-like peptide 1 (GLP-1) was observed. Unexpectedly, PAS reduced the concentrations of anti-amyloid beta (Aβ
1-42
) protein. Also, histopathological examination showed that PAS contributed to the reduction of STZ-lesioned pancreatic cells. Metformin treatment significantly reduced the diversity of the gut microbiota, while PAS treatment altered the diversity and composition of the microbiota. Collectively, our findings demonstrate that the hypoglycemic effects of PAS in type-2 diabetic mice (T2D) may be associated with the regulation of the intestinal microbiota and its metabolic pathways.
The hypoglycemic effects of PAS in type-2 diabetic mice (T2D) may be associated with the regulation of the intestinal microbiota and its metabolic pathways.
While treatments for childhood osteosarcoma have improved, the overall survival for this common type of bone cancer has not changed for three decades, and thus, new targets for therapeutic ...development are needed. To identify tumor‐related proteins in osteosarcoma, we used isobaric tags in a relative and absolute quantitation proteomic approach to analyze the differentially expressed proteins between osteosarcoma cells and human osteoblastic cells. Through clinical screening and functional evaluation, CCR4–NOT transcription complex subunit 1 (CNOT1) correlated with the growth of osteosarcoma cells. To date, the mechanisms and regulatory roles of CNOT1 in tumors, including osteosarcoma, remain largely elusive. Here, we present evidence that knockdown of CNOT1 inhibits the growth of osteosarcoma in vitro and in vivo. Mechanistically, we observed that CNOT1 interacted with LMNA (lamin A) and functioned as a positive regulator of this intermediate filament protein. The RNA‐seq analysis revealed that CNOT1 depletion inhibited the Hedgehog signaling pathway in osteosarcoma cells. A rescue study showed that the decreased growth of osteosarcoma cells and inhibition of the Hedgehog signaling pathway by CNOT1 depletion were reversed by LMNA overexpression, indicating that the activity of CNOT1 was LMNA dependent. Notably, the CNOT1 expression was significantly associated with tumor recurrence, Enneking stage, and poor survival in patients with osteosarcoma. Examination of clinical samples confirmed that CNOT1 expression positively correlated with LMNA protein expression. Taken together, these results suggest that the CNOT1–LMNA–Hedgehog signaling pathway axis exerts an oncogenic role in osteosarcoma progression, which could be a potential target for gene therapy.
Osteosarcoma is a common bone tumor in children and adolescents. A comparative proteomic analysis showed that the transcription factor subunit CNOT1 is overexpressed in osteosarcoma cells and clinical specimens. CNOT1 depletion inhibits osteosarcoma growth in vitro and in vivo. CNOT1 interacts with lamin A (LMNA) to activate the Hedgehog signaling pathway. CNOT1 expression is correlated with tumor recurrence, Enneking stage, and poor survival.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Atopic dermatitis (AD) is a common inflammatory skin disorder induced by dysfunction of immune suppression sharing similar pathogenesis to autoimmune diseases. To explore the association between ...autoimmune diseases and AD in children, we linked the birth data from National Birth Registry with National Health Insurance Research Database. There were 1,174,941 children obtained from 2006 to 2012 birth cohort. A total of 312,329 children diagnosed with AD before 5 years old were compared to 862,612 children without AD in the control group. Conditional logistic regression was utilized to calculate adjusted odds ratio (OR) and Bonferroni-corrected confidence interval (CI) for overall significance level of 0.05. In 2006–2012 birth cohort, the prevalence rate of AD was 26.6% (95% CI 26.5, 26.7) before 5 years of age. Having parental autoimmune disease (including rheumatoid arthritis, systemic lupus erythematosus, Sjogren’s syndrome, ankylosing spondylitis, and psoriasis) was associated with a significant higher risk of children AD development. The other associated factors were maternal obstetric complications (including gestational diabetes mellitus and cervical incompetence), parental systemic diseases (including anemia, hypertension, diabetes mellitus, chronic obstructive pulmonary disease, hyperthyroidism, and obstructive sleep apnea), and parental allergic disease (including asthma and AD). The subgroup analysis showed similar results between children’s sexes. Moreover, maternal autoimmune disease had higher impact on the risk of developing AD in the child compared with paternal autoimmune disease. In conclusion, parental autoimmune diseases were found to be related to their children’s AD before 5 years old.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Seven new complexes based on H4L1 and H4L2, namely, Cd2(L1)(H2O)5 n (1), {Cu5(L1)2(μ3-OH)2(H2O)4·2H2O} n (2), {Cd2(L1)(4,4′-bipy)2(H2O)3·2H2O} n (3), Zn2(L1)(4,4′-bipy)(H2O)3 n (4), ...{Ni7(L1)4(4,4′-bipy)12(H2O)12·2H2O} n (5), {Ni1.5(L2)(4,4′-bipy)1.5(H2O)4·H2O} n (6), and {Ni9(L2)6(dib)12(H2O)18·18H2O} n (7) (H4L1 = 4-(3′,5′-dicarboxylphenoxy)phthalic acid, H4L2 = 3-(3′,5′-dicarboxylphenoxy)phthalic acid, 4,4′-bipy = 4,4′-bipyridine, dib = 1,4-di(1H-imidazol-1-yl)benzene, have been synthesized by solvothermal reactions. Complex 1 possesses a 4-connected chiral three-dimensional (3D) structure with a Schläfli symbol of (4.63.82). Complex 2 presents a (4,6)-connected porous architecture based on a chair-shaped Cu4(μ3-OH)26+ secondary building unit (SBU) with a Schläfli symbol of (43.63)2(46.66.83), revealing unusual ferromagnetic behavior. Complex 3 exhibits a 2D → 3D framework through H-bonding interactions, which is a (3,3,4)-connected 2-fold interpenetrated structure with a Schläfli symbol of (6.82)2(63.83). Complex 4 shows a (3,4,6)-connected (4.64.8)2(62.8)2(42.66.86.10) topology. Complex 5 displays a rare (3,4)-connected polycatenation structure, possessing identical topologies with a Schläfli symbol of (63)(65.8) and weakly ferromagnetic behavior. Complex 6 features a (3,4)-connected 3D framework with a Schläfli symbol of (4 × 102)2(42.103.12). Complex 7 can be regarded as a (4,4)-connected (63.83)2(85.10) topology. Moreover, diverse structural topologies have not been documented hitherto. The magnetic properties of 2 and 5 are discussed in detail. The fluorescence properties have also been analyzed with density functional theory calculations, indicating that the emissions can be ascribed to intraligand charge transfer for 1 and ligand-to-ligand charge transfer for 3–4.
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IJS, KILJ, NUK, PNG, UL, UM
We constrain the mass of the Milky Wayʼs dark matter halo, based on the kinematics of 9627 K giants at Galactocentric distances ranging over 5 kpc < r < 120 kpc drawn from LAMOST DR5. The ...substructure in this sample has been identified and removed carefully to enable construction of the underlying line-of-sight velocity dispersion at different radii from the Galactic center. We interpret the radial profile of the line-of-sight velocity dispersion using a spherical Jeans equation under the assumptions of anisotropy/isotropy and that radial velocity dispersion is approximately equal to line-of-sight velocity dispersion σ r ( r ) ≈ σ los ( r ) . If we assume that the dark matter halo follows an NFW profile and the stellar halo is isotropic (β = 0), then σ los ( r ) can be directly used to estimate the virial mass of the Galactic dark matter halo, M vir = 1.08 − 0.14 + 0.17 × 10 12 M ⊙ , and concentration parameter c = 18.5 − 2.9 + 3.6 . In case that the stellar halo is anisotropic, we cannot avoid differentiation of sparse velocity dispersions according to the Jeans equation, which may cause overestimation of the mass. We use an isotropic case to test and find that d ln ( σ los 2 ( r ) ) / d ln r overestimates the virial mass by 15% but within 1-σ error. We use d ln ( σ los 2 ( r ) ) / d ln r to fit the NFW profile and get M vir = 1.11 − 0.20 + 0.24 × 10 12 M ⊙ and c = 13.8 − 2.2 + 3.0 in case of β = 0.3.
Heterogeneous Fenton-like catalyst prepared by dispersing iron oxides in activated carbon (FeOx@AC) has frequently been assembled for advanced oxidation processes (AOPs). An intriguing but barely ...emphasized property of FeOx@AC is that it can be easily reactivated through a simple carbothermal reaction. Importantly, by this manner the life time of FeOx@AC could be effectively enhanced. We herein reported the synthesis of FeOx@ACs hydrothermally with assistance of several commercially available surfactants and their performance in degrading real dye wastewater were evaluated. In general, as-synthesized FeOx@ACs were noted to equip high Fe content. Deposited FeOx reduced the fraction of micropores but simultaneously introduced additional mesopores and macropores. Elevated magnetite content was observed in FeOx@AC equipped with high fraction of micropore and mesopore and macropore but fast dye degradation occurred at FeOx@AC possessing low fraction of micropore along with low mesopores and macropores. Reactivation via carbothermal reaction redistributed the deposited FeOx by increasing micropores while decreasing mesopores and macropores. Importantly, well dispersed FeOx synthesized with the assistance of surfactants exhibited high resistance to the corrosion in the degradation process. For the perspective of circular economy, deep understanding the material chemistry of FeOx@AC would be of particularly interest for further enhancing its life time.
Display omitted
•Pursuing circular economy constantly drives the industry to design and utilize catalysts with enhanced life time.•The life time of FeOx@AC heterogeneous Fenton reagent could be greatly enhanced by a simple carbothermal reaction.•The pattern of how FeOx dispersing over AC surfaces would eventually regulate the life time of reactivated FeOx@AC.•Loosely bound FeOx over FeOx@AC surface was prone to dissociate apart and consequently resulted in Fe-rich sludge.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Taking 1420 mm UCM six-high cold rolling mill as the research object, a non square flatness control system with five input and four output is decoupled into a square subsystem with two input and two ...output which controls the primary and cubic flatness and a non square subsystem with three input and two output which controls the quadratic and quartic flatness by using the relative gain theory. By decomposing the unstable poles of the generalized inverse matrix of the non square system, the method of the generalized inverse matrix decoupling control the quadratic and quartic flatness is proposed, which solves the unstable problem of decoupling of non-square system. According to the characteristics of intermediate roll shifting, the variable model of roll shifting influence coefficient and the control strategy of minimum roll shifting adjustment and threshold are proposed. The dynamic characteristics of the system are improved and the adjustment of intermediate roll shifting is reduced. In order to overcome the shortcomings of low accuracy and poor generalization ability of shallow neural network, a mechanism-intelligent influence matrix model based on big data and deep neural network is proposed. Simulation calculation and industrial application show that the control system runs stably, the adjustment speed is fast, the control precision is high, the change of intermediate roll shifting is small, and it is suitable for online control.
Circularly polarized luminescence (CPL) with polychromatic colors has been widely studied due to its great applications in chiroptical, optoelectronics, and spintronics. However, the realization of ...white CPL in single‐component solid‐state materials remains a great challenge and suffers from the incompatibility between high efficient luminescence and large asymmetric discrimination. Here, by exploiting self‐trapped exciton mechanism and chirality induction strategy, a pair of 1D chiral perovskites, (RR/SS‐DMPZ)PbBr4 (where DMPZ = cis‐2,5‐dimethylpiperazine divalent cation), is reported to achieve white CPL with both high quantum yield of 28.4% and large photoluminescence asymmetry factor |glum| of 2.32 × 10−2. The crystal structures are featured by 1D double‐chain structure composed of distorted octahedra with short PbPb distances as the structural origin of the high photoluminescence yield. The high |glum| value is caused by efficient chiral induction due to the multiple hydrogen bonds between the chiral host composed of the enantiopure organic cations containing two stereocenters and the inorganic emitting guest. The self‐trapped exciton emission mechanism is demonstrated by density functional theory calculations and variable‐temperature photoluminescence and femtosecond‐transient absorption spectroscopy studies. The photo‐luminescent white light‐emitting diodes exhibit good stability and can be used as single‐component white light emitters. This work provides applicable strategies to explore single‐component white CPL emitters.
A pair of single‐component circularly polarized white light emitters with an ultra‐broadband emission are successfully synthesized. The white circularly polarized light emission originates from the self‐trapped exciton mechanism. The perovskite enantiomers with a double 1D chain exhibit excellent performance with a high quantum yield (28.4%) and large photoluminescence asymmetry factor (2.32 × 10−2).
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
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