Cerambycidae (longhorn beetles) and related families in the superfamily Chrysomeloidea are important components of forest ecosystems and play a key role in nutrient cycling and pollination. Using ...full mitochondrial genomes and dense taxon sampling, the phylogeny of Chrysomeloidea with a focus on Cerambycidae and allied families was explored. We used 151 mitochondrial genomes (75 newly sequenced) covering all families and 29 subfamilies of Chrysomeloidea. Our results reveal that (i) Chrysomelidae (leaf beetles) are sister to all other chrysomeloid families; (ii) Cerambycidae sensu stricto (s. s.) is polyphyletic due to the inclusion of other families that split Cerambycidae into a ‘lamiine’ clade comprising Lepturinae sensu lato (s. l.) + (Lamiinae + Spondylidinae) and a ‘cerambycine’ clade comprising Dorcasominae + (Cerambycinae + Prioninae s. l.); (iii) the subfamilies within the two clades of Cerambycidae s. s. were monophyletic, except for the placement of Necydalinae nested in Lepturinae, and the placement of Parandrinae within Prioninae (now considered as tribes Necydalini and Parandrini, respectively); (iv) smaller families were grouped into two major clades: one composed of Disteniidae+Vesperidae and the other composed of Orsodacnidae + (Megalopodidae + Oxypeltidae); (v) relationships among the four major clades were poorly supported but were resolved as ((cerambycines + (Disteniidae + Vesperidae) + Orsodacnidae + (Megalopodidae + Oxypeltidae)) + lamiines. Divergence time analyses estimated that Chrysomeloidea originated ca. 154.1 Mya during the late Jurassic, and most subfamilies of Cerambycidae originated much earlier than subfamilies of Chrysomelidae. The diversification of families within Chrysomeloidea was largely coincident with the radiation of angiosperms during the Early Cretaceous.
Seventy‐five full mitochondrial genomes were newly generated and combined with existing Genbank data, for a dataset of 151 mitochondrial genomes covering all families and 29 subfamilies of Chrysomeloidea.
The mitogenomes provide a framework for the phylogeny and classification of Chrysomeloidea, which confirm and extend existing notions of deep‐level relationships of Cerambycidae and the smaller families of Chrysomeloidea.
Divergence time analyses estimated that Chrysomeloidea originated ca. 154.1 Mya in the late Jurassic.
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DOBA, FZAB, GIS, IJS, IZUM, KILJ, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBMB, UILJ, UKNU, UL, UM, UPUK
Advanced persistent threat (APT) is a new kind of cyberattack that poses a serious threat to modern society. When an APT campaign on an organization has been identified, the available repair ...resources must be reasonably allocated to the potentially insecure hosts to mitigate the potential loss of the organization. We refer to the feasible repair resource allocation strategies as repair strategies. This paper focuses on the APT repair problem, i.e., the problem of developing effective repair strategies for organizations. First, for an organization with time-varying communication relationship, we establish an evolution model of the organization's expected state, in which the impact of lateral movement of APT is accommodated. On this basis, we model the APT repair problem as a differential Nash game problem (the APT repair game) in which the attacker attempts to maximize his potential benefit, and the organization manages to minimize its potential loss. Second, we derive a system (the potential system) for calculating a potential Nash equilibrium of an APT repair game, and we examine the structure of the potential attack and repair strategies in a potential Nash equilibrium. Next, we solve some potential systems to get the corresponding potential Nash equilibria. Finally, by comparison with a large number of randomly generated attack and repair strategies, we conclude that the potential Nash equilibrium of each APT repair game is a Nash equilibrium of the game. Therefore, we recommend to organizations their respective potential repair strategies. Our findings help to better understand and effectively defend against APT.
Although the preparation of TiO2 exposed with high-energy facets is a challenge, these facets have a large application potential in the loading of Au nanoparticles. We successfully prepared TiO2 ...single crystals exposed with (001) and (110) facets as an ideal support to load the highly dispersed nanosized Au particles to improve the stability of Au on the catalyst surface and expand its photocatalytic applications. The transfer of photoexcited electrons from the higher-surface-energy facet, that is, (001) and (110), to the lower-energy facet of (101) could make the electrons aggregate on the (101) facet and leave holes on the (001) and (110) facets, which would promote the separation of electrons and holes. The highly dispersed Au nanoparticles also could capture the electrons from active facets and further improve the photocatalytic activity of TiO2. Systematic density functional theory calculation was also carried out to investigate the formation of (001) and (110) facets.
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IJS, KILJ, NUK, PNG, UL, UM
Homologs of the human hepatitis E virus (HEV) have been identified in more than a dozen animal species. Some of them have been evidenced to cross species barriers and infect humans. Zoonotic HEV ...infections cause chronic liver diseases as well as a broad range of extrahepatic manifestations, which increasingly become significant clinical problems. Bats comprise approximately one-fifth of all named mammal species and are unique in their distinct immune response to viral infection. Most importantly, they are natural reservoirs of several highly pathogenic viruses, which have induced severe human diseases. Since the first discovery of HEV-related viruses in bats in 2012, multiple genetically divergent HEV variants have been reported in a total of 12 bat species over the last decade, which markedly expanded the host range of the HEV family and shed light on the evolutionary origin of human HEV. Meanwhile, bat-borne HEV also raised critical public health concerns about its zoonotic potential. Bat HEV strains resemble genomic features but exhibit considerable heterogeneity. Due to the close evolutionary relationships, bat HEV altogether has been recently assigned to an independent genus,
. This review focuses on the current state of bat HEV and provides novel insights into HEV genetic diversity and molecular evolution.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Since the outbreak of severe acute respiratory syndrome (SARS) 18 years ago, a large number of SARS-related coronaviruses (SARSr-CoVs) have been discovered in their natural reservoir host, bats
. ...Previous studies have shown that some bat SARSr-CoVs have the potential to infect humans
. Here we report the identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China. The epidemic, which started on 12 December 2019, had caused 2,794 laboratory-confirmed infections including 80 deaths by 26 January 2020. Full-length genome sequences were obtained from five patients at an early stage of the outbreak. The sequences are almost identical and share 79.6% sequence identity to SARS-CoV. Furthermore, we show that 2019-nCoV is 96% identical at the whole-genome level to a bat coronavirus. Pairwise protein sequence analysis of seven conserved non-structural proteins domains show that this virus belongs to the species of SARSr-CoV. In addition, 2019-nCoV virus isolated from the bronchoalveolar lavage fluid of a critically ill patient could be neutralized by sera from several patients. Notably, we confirmed that 2019-nCoV uses the same cell entry receptor-angiotensin converting enzyme II (ACE2)-as SARS-CoV.
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FZAB, GEOZS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Abstract
The bias‐stress instability of nanowires (NWs) field‐effect‐transistors (FETs), originated from the surface trappings, are challenging greatly the functionalization of III‐V group ...semiconductors in next‐generation electronics and optoelectronics. In this study, the solution‐processed high‐
κ
oxide dielectric shell is configured uniformly onto the surface of GaSb NWs, contributing to the excellent bias‐stress stability of as‐constructed p‐type NWFETs. Owing to the interdiffusion between Al and Ga, the oxide dielectric shell is Ga‐AlO
x
. With an optimal oxide dielectric shell, the as‐constructed p‐type GaSb NWFETs show an insignificant attenuation of on‐state current (within 10%) and a negligible negative shift of threshold voltage under 60 min continuous gate bias, which is far better than that of pristine GaSb NWFETs, resulting from the electric double layer effect. Benefiting from the excellent bias‐stress stability, when configured into the near‐infrared photodetector, NWFET exhibits desirable stability and gate‐controlled photodetection behaviors. I
dark
and I
light
are effectively modulated by gate voltage, resulting in gate‐controlled responsivity and gain under the illumination of 1550 nm laser. In the end, the as‐constructed bias‐stress stability NWFET demonstrates expected gate‐controlled photodetection imaging and photocommunication ability. The strategy of solution‐processed oxide dielectric shell promises high bias‐stress stability NWFETs for gate‐controlled photodetection and photocommunication.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
To address the safety and efficiency issues of vehicles at multi-lane merging zones, a cooperative decision-making framework is designed for connected automated vehicles (CAVs) using a coalitional ...game approach. Firstly, a motion prediction module is established based on the simplified single-track vehicle model for enhancing the accuracy and reliability of the decision-making algorithm. Then, the cost function and constraints of the decision making are designed considering multiple performance indexes, i.e. the safety, comfort and efficiency. Besides, in order to realize human-like and personalized smart mobility, different driving characteristics are considered and embedded in the modeling process. Furthermore, four typical coalition models are defined for CAVS at the scenario of a multi-lane merging zone. Then, the coalitional game approach is formulated with model predictive control (MPC) to deal with decision making of CAVs at the defined scenario. Finally, testings are carried out in two cases considering different driving characteristics to evaluate the performance of the developed approach. The testing results show that the proposed coalitional game based method is able to make reasonable decisions and adapt to different driving characteristics for CAVs at the multi-lane merging zone. It guarantees the safety and efficiency of CAVs at the complex dynamic traffic condition, and simultaneously accommodates the objectives of individual vehicles, demonstrating the feasibility and effectiveness of the proposed approach.
Driver attention estimation is one of the key technologies for intelligent vehicles. The existing related methods only focus on the scene image or the driver's gaze or head pose. The purpose of this ...article is to propose a more reasonable and feasible method based on a dual-view scene with calibration-free gaze direction. According to human visual mechanisms, the low-level features, static visual saliency map, and dynamic optical flow information are extracted as input feature maps, which combine the high-level semantic descriptions and a gaze probability map transformed from the gaze direction. A multiresolution neural network is proposed to handle the calibration-free features. The proposed method is verified on a virtual reality experimental platform that collected more than 550 000 samples and obtained a more accurate ground truth. The experiments show that the proposed method is feasible and better than the state-of-the-art methods based on multiple widely used metrics. This study also provides a discussion of the effects of different landscapes, times, and weather conditions on the performance.
COVID-19 has spread worldwide since 2019 and is now a severe threat to public health. We previously identified the causative agent as a novel SARS-related coronavirus (SARS-CoV-2) that uses human ...angiotensin-converting enzyme 2 (hACE2) as the entry receptor. Here, we successfully developed a SARS-CoV-2 hACE2 transgenic mouse (HFH4-hACE2 in C3B6 mice) infection model. The infected mice generated typical interstitial pneumonia and pathology that were similar to those of COVID-19 patients. Viral quantification revealed the lungs as the major site of infection, although viral RNA could also be found in the eye, heart, and brain in some mice. Virus identical to SARS-CoV-2 in full-genome sequences was isolated from the infected lung and brain tissues. Last, we showed that pre-exposure to SARS-CoV-2 could protect mice from severe pneumonia. Our results show that the hACE2 mouse would be a valuable tool for testing potential vaccines and therapeutics.
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•SARS-CoV-2 could infect HFH4-hACE2 mice and cause death•SARS-CoV-2 infection localizes to lungs of mice and causes typical interstitial pneumonia•Pre-exposure to SARS-CoV-2 protects mice from lethal challenge
A SARS-CoV-2 hACE2 transgenic mouse infection model recapitulates a number of infection symptoms and pathology in COVID-19 patients. Pre-exposure to SARS-CoV-2 was able to protect mice from severe pneumonia.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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