In this work, simple, rapid, and low-cost multiplexed detection of tumor-related micro-RNAs (miRNAs) was achieved based on multi-color fluorescence on a microfluidic droplet chip, which simplified ...the complexity of light path to a half. A four-T-junction structure was fabricated to form uniform nano-volume droplet arrays with customized contents. Multi-color quantum dots (QDs) used as the fluorescence labels were encapsulated into droplets to develop the multi-path fluorescence detection module. We designed an integrated multiplex fluorescence resonance energy transfer system assisted by multiple QDs (four colors) and one quencher to detect four tumor-related miRNAs (miRNA-20a, miRNA-21, miRNA-155, and miRNA-221). The qualitative analysis of miRNAs was realized by the color identification of QDs, while the quantitative detection of miRNAs was achieved based on the linear relationship between the quenching efficiency of QDs and the concentration of miRNAs. The practicability of the multiplex detection device was further confirmed by detecting four tumor-related miRNAs in real human serum samples. The detection limits of four miRNAs ranged from 35 to 39 pmol/L was achieved without any target amplification. And the linear range was from 0.1 nmol/L to 1 μmol/L using 10 nL detection volume (one droplet) under the detection speed of 320 droplets per minute. The multiple detection system for miRNAs is simple, fast, and low-cost and will be a powerful platform for clinical diagnostic analysis.
Graphical abstract
Full text
Available for:
DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
The influence of stress on the small‐signal dielectric permittivity and piezoelectric coefficient of polycrystalline lead‐free perovskite 0.92(Na1/2K1/2)NbO3–(0.08 − x)Bi1/2Li1/2TiO3–xBaZrO3 (x = 0, ...0.02, 0.04, 0.06, and 0.07) was characterized under different constant uniaxial stress up to −200 MPa within a temperature range of −150 to 450°C, revealing stress‐induced suppression of the electromechanical response as well as shifts in the phase boundaries. For all compositions, the interferroelectric and ferroelectric–paraelectric phase transitions were shifted to higher temperatures under the uniaxial compressive stress. Interestingly, the sensitivity to the applied stress was found to increase with increasing BZ/BLT ratio in the system. The origin of a different extent of stress‐sensitivity with BZ/BLT ratio is suggested to be related to the change in the crystal structure. Additionally, at temperatures below −50°C, the relative permittivity showed a significant increase under applied compressive stress.
Full text
Available for:
BFBNIB, DOBA, FZAB, GIS, IJS, IZUM, KILJ, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBMB, UILJ, UKNU, UL, UM, UPUK
Next-generation light-emitting displays on skin should be soft, stretchable and bright
. Previously reported stretchable light-emitting devices were mostly based on inorganic nanomaterials, such as ...light-emitting capacitors, quantum dots or perovskites
. They either require high operating voltage or have limited stretchability and brightness, resolution or robustness under strain. On the other hand, intrinsically stretchable polymer materials hold the promise of good strain tolerance
. However, realizing high brightness remains a grand challenge for intrinsically stretchable light-emitting diodes. Here we report a material design strategy and fabrication processes to achieve stretchable all-polymer-based light-emitting diodes with high brightness (about 7,450 candela per square metre), current efficiency (about 5.3 candela per ampere) and stretchability (about 100 per cent strain). We fabricate stretchable all-polymer light-emitting diodes coloured red, green and blue, achieving both on-skin wireless powering and real-time displaying of pulse signals. This work signifies a considerable advancement towards high-performance stretchable displays.
Full text
Available for:
EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Display omitted
•A review on current status of interfacial engineering enhances microbial EET.•The strengthening mechanisms of microbial EET are summarized.•Limitations of existing interfacial ...engineering methods are discussed.•Challenges and futuristic prospects of interfacial engineering are highlighted.
The extracellular electron transfer (EET) efficiency between electroactive microbes (EAMs) and electrode is a key factor determining the development of microbial electrochemical technology (MET). Currently, the low EET efficiency of EAMs limits the application of MET in the fields of organic matter degradation, electric energy production, seawater desalination, bioremediation and biosensing. Enhancement of the interaction between EAMs and electrode by interfacial engineering methods brings bright prospects for the improvement of the EET efficiency of EAMs. In view of the research in recent years, this mini-review systematically summarizes various interfacial engineering strategies ranging from electrode surface modification to hybrid biofilm formation, then to single cell interfacial engineering and intracellular reformation for promoting the electron transfer between EAMs and electrode, focusing on the applicability and limitations of these methodologies. Finally, the possible key directions, challenges and opportunities for future interfacial engineering to strengthen the microbial EET are proposed in this mini-review.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Transformer-customer relationship identification refers to the determination of the physical connection relationship of electricity end-customers and their corresponding transformers. Such connection ...relationship is critical for distribution utilities to maintain their end-customer profiles. However, management of transformer-customer relationship becomes one of the most emerging challenges due to large number of end-customers and lack of measurement devices in low-voltage distribution systems. To address the above issue, this paper proposes an end-customer data-driven method to identify transformer-customer relationship in low-voltage distribution grid by utilizing the customer field data obtained from advanced metering infrastructure. Specifically, the incidence convolution identification method is proposed to build up the unique mapping relationship between end-customers and their transformers based on the principle of energy conservation. Then the voltage correlation maximization model based on Markov Random Field is proposed, where the voltage correlation matrix is exacted and combined with the adjacency matrix to establish an optimization model to correct the potential abnormal transformer-customer relationship. Finally, the effectiveness of the proposed method is verified by using practical utility tests.
Abstract
Background
Streptomycetes from the rhizospheric soils are a rich resource of novel secondary metabolites with various biological activities. However, there is still little information ...related to the isolation, antimicrobial activity and biosynthetic potential for polyketide and non-ribosomal peptide discovery associated with the rhizospheric streptomycetes of
Panax notoginseng
. Thus, the aims of the present study are to (i) identify culturable streptomycetes from the rhizospheric soil of
P. notoginseng
by 16S rRNA gene, (ii) evaluate the antimicrobial activities of isolates and analyze the biosynthetic gene encoding polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) of isolates, (iii) detect the bioactive secondary metabolites from selected streptomycetes, (iv) study the influence of the selected isolate on the growth of
P. notoginseng
in the continuous cropping field. This study would provide a preliminary basis for the further discovery of the secondary metabolites from streptomycetes isolated from the rhizospheric soil of
P. notoginseng
and their further utilization for biocontrol of plants.
Results
A total of 42 strains representing 42 species of the genus
Streptomyces
were isolated from 12 rhizospheric soil samples in the cultivation field of
P. notoginseng
and were analyzed by 16S rRNA gene sequencing. Overall, 40 crude cell extracts out of 42 under two culture conditions showed antibacterial and antifungal activities. Also, the presence of biosynthesis genes encoding type I and II polyketide synthase (PKS I and PKS II) and nonribosomal peptide synthetases (NRPSs) in 42 strains were established. Based on characteristic chemical profiles screening by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD), the secondary metabolite profiles of strain SYP-A7257 were evaluated by High Performance Liquid Chromatography-High Resolution Mass Spectrometry (HPLC-HRMS). Finally, four compounds actinomycin X2 (
F1)
, fungichromin (
F2
), thailandin B (
F7
) and antifungalmycin (
F8
) were isolated from strain SYP-A7257 by using chromatography techniques, UV, HR-ESI-MS and NMR, and their antimicrobial activities against the test bacteria and fungus were also evaluated. In the farm experiments,
Streptomyces
sp. SYP-A7257 showed healthy growth promotion and survival rate improvement of
P. notoginseng
in the continuous cropping field.
Conclusions
We demonstrated the
P. notoginseng
rhizospheric soil-derived
Streptomyces
spp. distribution and diversity with respect to their metabolic potential for polyketides and non-ribosomal peptides, as well as the presence of biosynthesis genes PKS I, PKS II and NRPSs. Our results showed that cultivatable
Streptomyces
isolates from the rhizospheric soils of
P. notoginseng
have the ability to produce bioactive secondary metabolites. The farm experiments suggested that the rhizospheric soil
Streptomyces
sp. SYP-A7257 may be a potential biological control agent for healthy growth promotion and survival rate improvement of
P. notoginseng
in the continuous cropping field.
Full text
Available for:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The COVID-19 pandemic caused by SARS-CoV-2 has majorly impacted public health and economies worldwide. Although several effective vaccines and drugs are now used to prevent and treat COVID-19, ...natural products, especially flavonoids, showed great therapeutic potential early in the pandemic and thus attracted particular attention. Quercetin, baicalein, baicalin, EGCG (epigallocatechin gallate), and luteolin are among the most studied flavonoids in this field. Flavonoids can directly or indirectly exert antiviral activities, such as the inhibition of virus invasion and the replication and inhibition of viral proteases. In addition, flavonoids can modulate the levels of interferon and proinflammatory factors. We have reviewed the previously reported relevant literature researching the pharmacological anti-SARS-CoV-2 activity of flavonoids where structures, classifications, synthetic pathways, and pharmacological effects are summarized. There is no doubt that flavonoids have great potential in the treatment of COVID-19. However, most of the current research is still in the theoretical stage. More studies are recommended to evaluate the efficacy and safety of flavonoids against SARS-CoV-2.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
silkworm is an important economic insect which has a significant contribution to the improvement of the economy.
nucleopolyhedrovirus (BmNPV) is a vitally significant purulent virus that impedes the ...sustainable and stable development of the silkworm industry, resulting in substantial economic losses. In recent years, with the development of biotechnology, transcriptomics, proteomics, metabolomics, and the related techniques have been used to select BmNPV-resistant genes, proteins, and metabolites. The regulatory networks between viruses and hosts have been gradually clarified with the discovery of ncRNAs, such as miRNA, lncRNA, and circRNA in cells. Thus, this paper aims to highlight the results of current multi-omics and ncRNA studies on BmNPV resistance in the silkworm, providing some references for resistant strategies in the silkworm to BmNPV.
A highly selective, efficient and practical method for synthesizing primary alcohols was presented. By using cheap Fe(BF4)2 ⋅ 6H2O and /tris2‐(diphenylphosphino)phenylphosphine (L1) as catalysts with ...formic acid as hydrogen source, a variety of primary alcohols bearing versatile functional groups could be obtained from both alkyl‐ and aryl‐substituted epoxides under mild conditions.
Highly selective and efficient synthesis of primary alcohols from epoxides could be achieved under mild conditions by iron catalysis, this process used formic acid as hydrogen source and was suitable for both alkyl‐ and aryl‐substituted epoxides.
Full text
Available for:
BFBNIB, FZAB, GIS, IJS, KILJ, OILJ, SBCE, SBMB, UPUK
PDF
