A viral metagenomic analysis of feces from an unexplained outbreak of feline diarrhea revealed the presence of Lyon-IARC polyomavirus (LIPyV) DNA. LIPyV, whose genome was originally sequenced from ...swabs of human skin, was fecally shed by three out of five diarrheic cats.
Abstract Correction to: The ISME Journal (2014) 8, 1323–1335; doi:10.1038/ismej.2014.14; published online 20 February 2014 Since the publication of this article, the authors have identified two ...omissions within their paper, the main one being that the author affiliations were incomplete. The correct list is shown here.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, SBMB, SBNM, UL, UM, UPUK
Roseburia inulinivorans is a recently identified motile representative of the Firmicutes that contributes to butyrate formation from a variety of dietary polysaccharide substrates in the human large ...intestine. Microarray analysis was used here to investigate substrate-driven gene-expression changes in R. inulinivorans A2-194. A cluster of fructo-oligosaccharide/inulin utilization genes induced during growth on inulin included one encoding a β-fructofuranosidase protein that was prominent in the proteome of inulin-grown cells. This cluster also included a 6-phosphofructokinase and an ABC transport system, whereas a distinct inulin-induced 1-phosphofructokinase was linked to a fructose-specific phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS II transport enzyme). Real-time PCR analysis showed that the β-fructofuranosidase and adjacent ABC transport protein showed greatest induction during growth on inulin, whereas the 1-phosphofructokinase enzyme and linked sugar phosphotransferase transport system were most strongly up-regulated during growth on fructose, indicating that these two clusters play distinct roles in the use of inulin. The R. inulinivorans β-fructofuranosidase was overexpressed in Escherichia coli and shown to hydrolyze fructans ranging from inulin down to sucrose, with greatest activity on fructo-oligosaccharides. Genes induced on starch included the major extracellular α-amylase and two distinct α-glucanotransferases together with a gene encoding a flagellin protein. The latter response may be concerned with improving bacterial access to insoluble starch particles. PUBLICATION ABSTRACT
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BFBNIB, NMLJ, NUK, PNG, SAZU, UL, UM, UPUK
TESTED THE GENERALIZATION THAT INCREASED DRIVE IS ASSOCIATED WITH A REDUCTION IN THE RANGE OF CUE UTILIZATION. IN EXP. I THE PRESENCE OR ABSENCE OF AN AUDIENCE WAS USED TO MANIPULATE DRIVE, WHILE IN ...EXP. II TASK SUCCESS OR FAILURE WAS EMPLOYED. THE RESULTS OF BOTH EXPERIMENTS INDICATED THAT THE EFFECTS OF CUES, TRIALS, AND THE TRIALS * CUES * DRIVE INTERACTION WERE ALL SIGNIFICANT. THE FINDINGS OF BOTH STUDIES DEMONSTRATE THAT SOCIAL MANIPULATION OF DRIVE LEVEL AFFECTS THE RANGE OF CUE UTILIZATION.
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CEKLJ, FFLJ, NUK, ODKLJ, PEFLJ, UPUK
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