Neurodegenerative diseases (NDD) are typically associated with neuron loss in nervous system areas. Interventions with related death mechanisms may ameliorate NDD progression. Oxidative stress plays ...an important role in NDD cell death routines. However, tert-butylhydroperoxide (t-BHP), a widely used oxidative stress stimulus, induces neural cell death through a mechanism that remains elusive. In our study, the ferroptosis marker events occurred after co-treatment with 100 μM t-BHP for 1 h, all of which were reversed in the presence of the ferroptosis inhibitor ferrostatin-1 (Fer-1) and the iron chelator deferoxamine, implying the occurrence of ferroptosis. Moreover, mitochondrial dysfunction accompanied by a decreased in membrane potential and ATP production, increased mitochondrial ROS generation. Furthermore, this mitochondrial dysfunction could be reversed by Fer-1. In addition, JNK1/2 and ERK1/2 were activated upstream of the ferroptosis and mitochondrial dysfunction. In summary, these data suggest that ferroptosis, coupled with mitochondrial dysfunction, was involved in t-BHP-induced PC12 death. JNK1/2 and ERK1/2 played important roles in t-BHP-induced cell death. Overall, this study might provide clues to the oxidative stress-based strategies for cell protection in NDD.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
The treatment of tenacious diabetic wounds still remains an enormous challenge in clinics, originated from the complicated pathological microenvironment of wound sites. Therefore, it's urgently ...required to develop one type of innovative dressing patch with appropriate microstructure and multifunctions to regulate the pathological microenvironment and promote the regeneration of diabetic wounds. In this study, novel gelatin (Gel)/poly (L-lactic acid) (PLLA) nanofibrous yarns loading with or without Salvia miltiorrhiza Bunge-Radix Puerariae herbal compound (SRHC) are fabricated by using our modified electrospinning strategy, which are further interlaced into nanofibrous woven fabrics respectively, serving as biofunctional dressing patches for potential diabetic wound treatment application. The actual photographs and SEM images confirm that all the different nanofibrous textiles with or without SRHC exhibit a uniform interwoven structure of warp and weft, and the internal nanofibers present bead-free morphology and uniaxially oriented structure along the longitudinal axis of nanofibrous yarns. Moreover, all the different nanofibrous woven fabrics are demonstrated to possess strong mechanical properties and great surface wettability. The in vitro cell characterization shows that the addition of SRHC can significantly promote the attachment and proliferation of human dermal fibroblasts (HDFs), and also dramatically inhibit the secretion levels of proinflammatory factors of M1 macrophages. The in vivo diabetic mouse full-thickness skin model experiments reveal that the as-developed SRHC-loaded Gel/PLLA nanofibrous textile shows the best performances referring to shorten wound healing time (100 % wound closure after 18 days of treatment) and high-quality regeneration (i.e., enhance collagen deposition, improve re-epithelialization and neovascularization, and increase hair follicles), which assuredly finds great interests serving as an innovative dressing patch for the treatment of hard-to-heal diabetic wounds.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Psoralea corylifolia Linn. (P. corylifolia) is an important medicinal plant with thousands of years of clinical application. It has been widely used in many traditional Chinese medicine formulas for ...the treatment of various diseases such as leucoderma and other skin diseases, cardiovascular diseases, nephritis, osteoporosis, and cancer. Phytochemical studies indicated that coumarins, flavonoids, and meroterpenes are the main components of P. corylifolia, and most of these components are present in the seeds or fruits. The extracts and active components of P. corylifolia demonstrated multiple biological activities, including estrogenic, antitumor, anti-oxidant, antimicrobial, antidepressant, anti-inflammatory, osteoblastic, and hepatoprotective activities. This paper systematically summarized literatures on the chemical constituents and biological activities of P. corylifolia, which provided useful information for the further research and development toward this potent medicinal plant.
Developing bio-multifunctional wound dressings with excellent hemostasis, antibacterial, anti-inflammatory, enhanced angiogenesis and hair follicle regeneration for promoting full-thickness wound ...healing is highly valuable in clinical application. Herein, bio-multifunctional composite sponges were prepared by coupling alginate and chitosan with fucoidan through electrostatic interaction, Ca2+ crosslinking, and lyophilization processes. The prepared alginate/chitosan/fucoidan (ACF) sponges display excellent elastic properties, preserving their shape even after bending and compressive strain without failure. The ACF sponge containing 10% fucoidan (ACF-1) shows better hemostatic and antibacterial performances, and significantly promotes wound closure in a rat full-thickness wounds model compared to the alginate/chitosan group and ACF sponge containing 30% fucoidan. Furthermore, the ACF-1 sponge greatly facilitates the epithelialization and the formation of collagen in the dermis, promotes the hair follicle regeneration, enhances vascularization by upregulating the protein expression of CD31, and reduces inflammation by downregulating the protein expression of TNF-α. This work suggests that ACF sponges with a certain amount of fucoidan display great potential for the treatment of full-thickness skin repair.
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•Bio-multifunctional ACF sponges were successfully prepared through a facile, efficient, green cross-linked, and freeze-drying processes.•The ACF sponges possessed excellent hemostatic and antibacterial performances as well as greatly promoted full-thickness wound healing.•The ACF sponges reduced inflammation, facilitated the epithelialization, as well as enhanced angiogenesis and hair follicle regeneration.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Bio-textiles have aroused attractive attentions in tissue engineering and regenerative medicine, and developing robust, bio-absorbable, and extracellular matrix (ECM) fibril-mimicking nanofibrous ...textiles is urgently required for the renewal of existing microfibrous textile-based scaffolds and grafts. In this study, an integrated electrospinning system consisting of one nanoyarn-forming unit and one hot stretching unit is reported to fabricate silk fibroin (SF)/poly (L-lactic-acid) (PLLA) nanofibrous yarns (nanoyarns). The hot stretching process is demonstrated to significantly improve the fiber alignment, crystallinity, and mechanical properties of SF/PLLA nanoyarns, compared to the unstretched controls. For instance, the fiber alignment degree of hot stretched 50/50 SF/PLLA nanoyarn has increased by 25%, and the failure strength has increased by 246.5%, compared with the corresponding un-stretched control. Increasing the SF/PLLA mass ratio is found to significantly decrease the crystallinity and mechanical properties, but notably increase the degradation rate and surface hydrophilicity of SF/PLLA nanoyarns. Different SF/PLLA nanoyarns are further meticulously interwoven with warp and weft directions to obtain several nanofibrous woven textiles. The results from in vitro cell characterization and in vivo subcutaneous implantation show that increasing the SF/PLLA mass ratio significantly improves the biological properties and effectively reduces the inflammatory response of nanoyarn-constructed textiles. Overall, this study demonstrates that our SF/PLLA nanoyarns with controllable physical, mechanical and biological performances are fantastic candidates for the designing and development of advanced nanoarchitectured textile tissue scaffolds.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
External and internal stimuli are often involved in the pathogenesis of tumors, and the deterioration of endoplasmic reticulum (ER) function within cells is also an important etiological factor of ...tumorigenesis resulting in the impairment of the endoplasmic reticulum, which is termed ER stress. The ER is an organelle that serves a crucial role in the process of protein synthesis and maturation, and also acts as a reservoir of calcium to maintain intracellular Ca2+ homeostasis. ER stress has been revealed to serve a critical role in tumorigenesis. In the present review, the association between ER stress‑related pathways and tumor cell apoptosis is examined. Primarily, the role of ER stress in tumor cell apoptosis is discussed, and it is stipulated that ER stress, induced by drugs both directly and indirectly, promotes tumor cell apoptosis.
Recently, optical orthogonal frequency-division multiplexing technology has attracted intensive research interest because spectrum-sliced elastic optical networks (EONs) can be constructed based on ...it. In this paper, we investigate how to serve multicast requests over EONs with multicast-capable routing, modulation level, and spectrum assignment (RMSA). Both EON planning with static multicast traffic and EON provisioning with dynamic traffic are studied. For static EON planning, we formulate two integer linear programming (ILP) models, i.e., the joint ILP and the separate ILP. The joint ILP optimizes all multicast requests together, while the separate ILP optimizes one request each time in a sequential way. We also propose a highly efficient heuristic that is based on an adaptive genetic algorithm (GA) with minimum solution revisits. The simulation results indicate that the ILPs and the GA provide more efficient EON planning than the existing multicast-capable RMSA algorithms that use the shortest path tree (SPT) and the minimal spanning tree (MST). The results also show that the GA obtains more efficient EON planning results than the separate ILP with much less running time, as it can optimize all multicast requests together in a highly efficient manner. For the dynamic EON provisioning, we demonstrate that the GA is also applicable, and it achieves lower request blocking probabilities than the benchmark algorithms using SPTand MST.
•SOC stability was determined using TG50 and DSC.•SOC stability decreased with the elevation at p < 0.05 level.•SOC stability was controlled by TP, alkanes, Mn, Th, aliphatic, lignin, and ...phenol.•Soil nutrients and molecular features but not minerals controlled SOC stability.
The mechanisms that maintain soil organic carbon (SOC) stability along the elevation gradient has not been adequately explored in mountain ecosystems, though numerous work has been performed to reveal potential impacts of multiple environmental factors on SOC stability. The objective of this study was to decipher the predominant factor and effects that affected SOC stability by linking elevation, soil nutrients, minerals, and chemical molecular composition with SOC thermal stability in Changbai Mountain (CBM), Northeast China. Results indicated that SOC ranged from 2.12% to 17.75% without obvious elevation dependency. SOC stability was evaluated using the temperature at which haft of the exothermic mass loss (TG50). The TG50 decreased with elevation at p < 0.05 level. SOC did not vary significantly across soils from predominant plant communities, which were the mixed coniferous and broad-leaved forests (HJL), mixed coniferous forests (ZYL), birch forests (YHL), and tundra vegetation zones (TYD). Total phosphorus (P) and manganese (Mn) differed significantly among these four zones. Aliphatic, alkanes, alkenes, and aromatics were predominant moieties of pyrolytic products of SOC in all zones. TP, alkanes, Mn, Th, aliphatic, Lg, and Ph were the main drivers controlling SOC in contents and stabilities. Mineral protection in conjunction with soil nutrients could explain 73% of the total variance in SOC stability, while Mn had more importance than Fe in stabilizing organic carbon in CBM. The structural equation model revealed positive effects of both mineral protection and molecular feature on SOC pool and stability.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
To predict aeroheating performance of hypersonic vehicles accurately in thermochemical nonequilibrium flows accompanied by rarefaction effect, a Nonlinear Coupled Constitutive Relations (NCCR) model ...coupled with Gupta’s chemical models and Park’s two-temperature model is firstly proposed in this paper. Three typical cases are intensively investigated for further validation, including hypersonic flows over a two-dimensional cylinder, a RAM-C II flight vehicle and a type HTV-2 flight vehicle. The results predicted by NCCR solution, such as heat flux coefficient and electron number densities, are in better agreement with those of direct simulation Monte Carlo or flight data than Navier-Stokes equations, especially in the extremely nonequilibrium regions, which indicates the potential of the newly-developed solution to capture both thermochemical and rarefied nonequilibrium effects. The comparisons between the present solver and NCCR model without a two-temperature model are also conducted to demonstrate the significance of vibrational energy source term in the accurate simulation of high-Mach flows.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Currently, little is available on how mercury (Hg) methylation couples with soil organic matter decomposition in degraded permafrost in high northern latitudes, where the climate is becoming warmer ...rapidly. Here, we revealed the complex interactions between soil organic matter (SOM) mineralization, dissolved organic matter (DOM) and methylmercury (MeHg) production based on an 87-day anoxic warming incubation experiment. Results supported remarkably promotion effects of warming on MeHg production, by 130%–205% on average. Total mercury (THg) loss under warming treatment depended on marsh types but showed an increasing trend on the whole. Warming yielded higher proportions of MeHg to THg (%MeHg), increased by 123%–569%. As expected, greenhouse gas emission was significantly enhanced by warming. Warming also strengthened fluorescence intensities of fulvic-like and protein-like DOM, with contributions to total fluorescence intensities of 49%–92% and 8%–51%, respectively. DOM and its spectral features explained 60% variation of MeHg, and the explanation increased to 82% in conjunction with greenhouse gas emissions. The structural equation model implied that warming, greenhouse gas emission, and humification of DOM had positive effects on Hg methylation potential, while microbial-derived DOM showed negative effects on MeHg. These results showed that accelerated Hg loss and increased methylation covaried with greenhouse gas emission and DOM formation under warming conditions in permafrost marsh.
By stimulating greenhouse gas emission and altering fluorescence features of dissolved organic matter, warming remarkably fueled MeHg production and triggered mercury loss in permafrost marsh soils in Northeast China. Display omitted
•Warming promotes MeHg contents by 130%–205% in permafrost soils.•Warming causes more Hg loss and increased methylation covarying with SOM mineralization.•DOM and its spectral features predominate MeHg variation under warming.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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