Automatic detection of building edge information from high-resolution remote sensing images can more accurately obtain building distribution information, which is of great significance for urban land ...planning, urban building planning and population estimation. The satellite image will be affected by atmospheric interference and noise, we preprocess the image to eliminate the atmospheric interference such as atmospheric correction and radiometric calibration, and utilize a new total variation and wavelet adaptive thresholding hybrid filter proposed in this paper to achieve the filtering of noise while retaining the image edge detail information. The traditional detection operator is prone to the problems of scattered edge points, discontinuity or misjudgment of too many edge points in the detection results due to the limitations of directions, template sizes. Therefore, this paper proposes a new 16-direction <inline-formula> <tex-math notation="LaTeX">5\times 5 </tex-math></inline-formula> size Sobel operator to replace the previous Sobel operator with only horizontal and vertical directions, using these 16 directions to calculate the gradient of each pixel points. And each direction template is extracted to each direction contour image for weighted fusion. Thus, the edge information of different angles can be extracted, and the all-round information edge extraction of the image can be realized. By comparing the experimental visual effect and the evaluation criteria of the data results, the SNR, PSNR, AUC, and FOM values of the improved algorithm are much higher than other algorithms, with the advantage of higher image detection edge positioning accuracy, more complete contour lines and stronger anti-interference ability.
Abstract
Background
N6-methyladenosine (m6A) is the most common RNA modification, which plays a pivotal role in tumor development and progression. In this study, we assessed the role of m6A ...methyltransferase METTL3 in FRAS1-involved cell proliferation and colony formation of non-small cell lung cancer (NSCLC) cell lines.
Methods
Cell viability was analyzed by Cell Counting Kit (CCK-8) and colony formation. M6A RNA immunoprecipitation (IP), Ribosomal immunoprecipitation, RNA immunoprecipitation (RIP) were performed to verify the relationship between METTL3, FRAS1 and YTHDF1. Rescue experiments to confirm the regulatory mechanism of METTL3-FRAS1 promoted NSCLC cell proliferation through CDON by cooperating YTHDF1.
Results
We found that FRAS1 was correlated with poor prognosis in NSCLC patients, of which the transcript undergoes m6A modification regulated by METTL3. METTL3 silence reduced cell viability of NSCLC cells HCC827 and NCI-H1975, which could be restored by FRAS1 overexpression. The m6A modification of FRAS1 could be recognized by YTHDF1. FRAS1 silence or YTHDF1 silence could rescue the elevated NSCLC cell proliferation, colony formation, and tumor growth induced by METTL3 overexpression in vitro and in vivo.
Conclusions
Our study reveals that METTL3-FRAS1 plays a crucial role in NSCLC cell proliferation, colony formation, and tumor growth through the regulation of CDON by cooperating YTHDF1.
The rotational Doppler effect (RDE) associated with orbital angular momentum (OAM) has been used for remote sensing of a spinning object. However, one of the challenges of long-range detection stems ...from the low echo signal power. In this paper, we propose a new detection scheme that uses the combined vortex beam (CVB) generated by coherent beam combining (CBC) technology as the probe beam to enhance the echo signal power. Furthermore, we establish a rotational speed remote sensing model based on RDE, the emitted power and emission diameter of the probe beam are investigated in detail. The results show that, compared with the superposition vortex beam (SVB) generated by a single laser beam, the CVB detection scheme can significantly enhance the echo signal intensity and detection distance. The measuring range and accuracy of rotational speed are also studied in detail. And finally, we present the first experimental demonstration of the RDE arising directly from the interaction of the CVB with a rotating rough surface. The scheme proposed in our paper offers a good reference for practical application of the remote detection based on RDE.
On-chip topological nanophotonic filtering and routing devices are important components of integrated nanophotonic chip. The device filters topological photonic states with multiple frequencies and ...routes them into different output ports, respectively. However, there is no effective method to implement such devices to date. Here, an on-chip topological five-channel filtering and routing device is realized for the first time. Topological edge states are constructed based on synthetic dimension, which can use dielectric materials to construct topological photonic states without external magnetic field. This method has advantages including simple structure, easy integration, and high signal-to-noise ratio. The implementation of on-chip topological multichannel filtering and routing device is of great significance to the development of topological nanophotonic devices.
Wind parameters play a vital role in studying atmospheric dynamics and climate change. In this study, a vehicle-mounted coherent wind measurement Lidar (CWML) with a wavelength of 1.55 µm is ...demonstrated based on a four-beam vertical azimuth display (VAD) scanning mode, and a method to estimate wind vector from power spectrum is proposed. The feasibility of the application of wind profile Lidar in vehicles is verified by calibration tests, comparison experiments, and continuous observation experiments, successively. The effective detection height of Lidar can reach 3 km. In contrasting experiments, the correlation coefficients of the magnitude and direction of horizontal wind speed measured by vehicle-mounted Lidar and fixed Lidar are 0.94 and 0.91, respectively. The experimental results reveal that the accuracies of wind speed and direction measurements with the vehicle-mounted CWML are better than 0.58 m/s and 4.20°, respectively. Furthermore, to understand the role of the wind field in the process of energy and material transport further, a proton-transfer reaction time-of-flight mass spectrometer (PTR-TOF-MS) is utilized to measure the concentration of volatile organic compounds (VOCs). Relevant experimental results indicate that the local meteorological conditions, including wind speed and humidity, influence the VOC concentrations.
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In this letter, tin disulfide (SnS 2 ) nanosheets were employed as saturable absorber for obtaining passively mode-locked Yb-doped fiber laser. A SnS 2 -PVA film was fabricated by incorporating the ...SnS 2 nanosheets with the polyvinyl alcohol (PVA), Based on the SnS 2 -PVA film as saturable absorber, passively mode-locked Yb-doped fiber laser at a central wavelength of 1062.66 nm was achieved. The 3-dB bandwidth, pulse width, pulse repetition rate, and maximum average output power were 8.63 nm, 656 ps, 39.33 MHz, and 2.23 mW, respectively. The results exhibit that SnS 2 has excellent saturable absorption properties, which are equivalent to the reported two-dimensional transition metal dichalcogenides (WS 2 , MoS 2 ) and will have wide potential applications in the field of ultrafast photonics.
We experimentally investigate the formation of various pulses from a thulium–holmium (Tm–Ho)-codoped nonlinear polarization rotation (NPR) mode-locking fiber oscillator. The ultrafast fiber ...oscillator can simultaneously operate in the noise-like and soliton mode-locking regimes with two different emission wavelengths located around 1947 and 2010 nm, which are believed to be induced from the laser transition of Tm3+ and Ho3+ ions respectively. When the noise-like pulse (NLP) and soliton pulse (SP) co-exist inside the laser oscillator, a maximum output power of 295 mW is achieved with a pulse repetition rate of 19.85-MHz, corresponding to a total single pulse energy of 14.86 nJ. By adjusting the wave plates, the fiber oscillator could also deliver the dual-NLPs or dual-SPs at dual wavelengths, or single NLP and single SP at one wavelength. The highest 61-order harmonic soliton pulse and 33.4-nJ-NLP are also realized respectively with proper design of the fiber cavity.
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Single-pixel imaging (SPI) is a new type of imaging technology that uses a non-scanning single-pixel detector to image objects and has important application prospects and value in many fields. Most ...of the modulators currently used in SPI systems are digital micromirror device (DMD) modulators, which use a higher frequency for binary modulation than other alternatives. When modulating grayscale information, the modulation frequency is significantly reduced. This paper conducts research on multiple discrete objects in a scene and proposes using binary patterns to locate and image these objects. Compared with the existing methods of using gray patterns to locate and image multiple objects, the method proposed in this paper is more suitable for DMD-type SPI systems and has wider applicability and greater prospects. The principle of the proposed method is introduced, and the effectiveness of the method is experimentally verified. The experimental results show that, compared to traditional SPI methods, the number of patterns required by the proposed method is reduced by more than 85%.
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We propose an all-dielectric nanorod array for ultra-sensitive refractive index sensing based on quasi-bound states in the continuum (BICs). The nanorod is fabricated by silicon or silicon with an ...air hole, i.e., the hollow silicon nanorod. The quasi-BICs are formed in the hollow silicon nanorod array due to the symmetry-breaking of air holes. The high-quality factor (Q-factor) and ultra-narrow reflectance spectral width at quasi-BICs contribute to high performances of the sensor. The numerical results show that the sensitivity and figure of merit (FOM) can reach up to 602.9 nm/RIU and 34,977, respectively. The results indicate that the proposed nanostructures of quasi-BICs are promising for advanced biosensing applications.
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