This study evaluated the effects of the probiotic
Bacillus
spp. isolated from African nightcrawler (
Eudrilus eugeniae
) on the growth and survival performances of Nile tilapia (
Oreochromis ...niloticus
). A total of 400 Nile tilapia fingerlings (1.76 ± 0.07 g) were equally and randomly distributed into 20 glass aquaria and divided into five experimental groups with four replicates. Each experimental group was fed with a commercial diet (control) supplemented (at 10
8
CFU·g
−1
) with one of the four
Bacillus
spp. (ANSCI9, BFAR9, RM3, and RM10) at five percent of their body weight. After 30 days of feeding, the surviving fish were challenged with
Aeromonas hydrophila
to observe their survival. The treatment groups supplemented with BFAR9 (2.73 ± 0.26 g) and RM10 (3.15 ± 0.30 g) showed significantly higher (
P
< 0.05) absolute growth than the control (2.20 ± 0.16 g) group. Furthermore, those given with RM10 had better (
P
< 0.05) specific growth rate (SGR) (1.60 ± 0.10%·day
−1
) relative growth rate (RGR) (181.39 ± 18.16%) and feed conversion ratio (FCR) (1.99 ± 0.13) compared to the control group (SGR = 1.29 ± 0.07%·day
−1
; RGR = 129.84 ± 9.77%; FCR = 2.60 ± 0.16). The challenge test revealed that all
Bacillus
spp.-treated groups showed significantly higher (
P
< 0.05) survival rates (ANSCI9 = 82.5 ± 8.16%; BFAR9 = 80.0 ± 10.00%; RM3 = 77.5 ± 20.82%; RM10 = 85.0 ± 10.00%) than the control group (55.00 ± 19.15%), with the highest relative level of protection recorded for RM10 (66.67%). These results revealed that the probiotic
Bacillus
spp. isolated from
E. eugeniae
improved the growth, feed utilization, waste excretion, and the disease resistance of Nile tilapia.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Background. The primary motor cortex of the hand (M1-Hand) is a target used in transcranial magnetic stimulation (TMS) and in transcranial direct current stimulation (tDCS) for the treatment and ...evaluation of motor neurological diseases. Magnetic resonance imaging–guided neuronavigation locates the M1-Hand with high precision, but at a high cost. Although less accurate, the C3/C4 points of the international 10-20 system (IS 10-20) are routinely used to locate the M1-Hand. The international 10-5 system (IS 10-5) was developed with additional points (C3h/C4h), which could make it more accurate, but has not yet been tested on the location of the M1-Hand. Objective. To analyze and compare the accuracy of C1/C2, C3h/C4h and C3/C4 points in locating the M1-Hand correspondence on the scalp. Methods. The authors comparatively analyzed the distances from points C1/C2, C3h/C4h, and C3/C4 to the correspondence of the M1-Hand on the scalp in 30 MRI head exams. Results. In most cases, the M1-Hand was located between C1-C3h and C2-C4h in the left and right hemispheres of the brain, respectively. The C3h (0.98 ± 0.49 cm) and C4h (0.98 ± 0.51 cm) points presented the shortest distances from the M1-Hand, with a significant difference when compared with C3/C4. The accuracy between C1/C2 and C3h/C4h was not statistically significant. Conclusion. The C3h/C4h and C1/C2 points were more accurate when compared with the C3 and C4 points in locating the M1-Hand correspondence on the scalp.
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NUK, OILJ, SAZU, UKNU, UL, UM, UPUK
4.
Cuatro textos sobre el Parlamento Obrero Marx, Karl; Espinoza Pino, Mario
Azafea : estudios de historia de la filosofía hispánica,
11/2023, Volume:
25
Journal Article
Peer reviewed
Open access
Durante los años 1853 y 1854 surgió una ola de huelgas en varios distritos industriales de Inglaterra, especialmente en los sectores textiles de Lancashire y Manchester. Este ciclo de luchas, que ...pugnaba principalmente por un aumento de los salarios, fue visto por Ernest Jones y el movimiento Cartista como una oportunidad para organizar una asociación obrera a escala nacional. Karl Marx, que compartía la visión de los cartistas, analizó el movimiento desde el New York Tribune, brindando apoyo a la iniciativa a la que dio lugar: el Parlamento Obrero.
A continuación, se ofrece la traducción de cuatro textos periodísticos que Marx publicó en apoyo a la institución obrera –la mayoría inéditos en español–, seguidos de «Las luchas por la organización obrera en la época victoriana (1853-1854)», un estudio crítico sobre el ciclo de huelgas y las expectativas de organización obrera durante aquellos años, donde se defiende que este fallido intento de organización constituye un precedente de la sección británica de la Asociación Internacional de Trabajadores. La traducción y edición de los textos de Marx, y el estudio crítico que los acompaña, han sido realizados por Mario Espinoza Pino (Universidad de Granada).
The nematodes of the genus Anisakis are among the most relevant parasitic hazards in fishery products since they are responsible for human infection and allergy cases. In a food safety and ...epidemiological perspective, several marine hosts from different locations around Japan were examined to characterize the parasitism of Anisakis larvae. Chum salmon (Oncorhynchus keta) and Alaska pollock (Gadus chalcogrammus) showed the highest overall prevalence (100%), followed by blue mackerel (Scomber australasicus) (97.5%), Pacific cod (Gadus macrocephalus) (80%), chub mackerel (Scomber japonicus) (60.1%), Japanese flying squid (Todarodes pacificus) (17%) and Japanese pilchard (Sardinops sagax melanostictus) (2%). In Pacific krill (Euphausia pacifica), apart from one Hysterothylacium aduncum larva, no Anisakis specimens were detected. Anisakis simplex sensu stricto was molecularly identified (PCR-RFLP) for the first time in Japanese flying squid and Japanese pilchard distributed in the Northwestern Pacific ocean. That was the most frequent parasitic species detected followed by A. pegreffii, mostly in the western areas of Japan, hybrid genotypes between the two sibling species as well as A. typica and A. berlandi. Surprisingly, A. simplex s.s. was the most abundant species in one batch of chub mackerel from the East China Sea and A. pegreffii was the main species found in one batch from the Pacific coast of Aomori, which seems to indicate that the ranges of these two sibling species might be more variable than previously thought.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Cetaceans are definitive hosts of anisakid nematodes known to cause human anisakidosis. Despite the reported strandings of different cetaceans in the Philippines, studies on anisakids from these ...definitive hosts are limited. Here, the morphologically and molecularly identified anisakid species, specifically those of the genus
Anisakis
Dujardin, 1845 in stranded Pygmy Sperm Whale
Kogia breviceps
Blainville, 1838 in the west Pacific region off Philippine waters are presented. Morphological data using SEM and LM revealed multi-infections with different
Anisakis
species belonging to
Anisakis
type I and type II groups. Molecularly, PCR-RFLP on the ITS rDNA and sequence data analyses of both ITS rDNA and mtDNA cox2 regions identified those from
Anisakis
type I group as
A. typica
(Diesing, 1860), whereas those from type II group as
A. brevispiculata
Dollfus, 1968, and
A. paggiae
Mattiucci et al. (Syst Parasitol 61:157–171,
2005
). This is the first record of
Anisakis
infection from this host stranded in the west Pacific region off the coast of Philippine waters and new geographical record for
A. paggiae
.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Earthworms are used as an alternative protein source in aquaculture. These organisms serve as an ideal and favorable site for bacterial growth and activity. Hence, in our present study, we isolated ...and screened potential probiotic bacteria from African nightcrawler (
Eudrilus eugeniae
). Among 45 bacterial isolates, four (ANSCI9, BFAR9, RM3, and RM10) were selected based on their hydrophobicity, hydrolytic enzyme production, pH and fish bile tolerance, aggregation, and antimicrobial properties. The selected isolates showed good hydrophobicity (≥ 30%) and enzyme production (≥ 10 mm clearing zones), tolerance to pH and fish bile, and inhibitory properties against pathogenic microorganisms. The isolates were identified as
Bacillus
sp. RM3 (MH919306),
Bacillus
sp. RM10 (MH919308),
Bacillus
sp. ANSCI9 (MH919310) and
Bacillus
sp. BFAR9 (MH919302). These isolates were individually incorporated in the diets of Nile tilapia (
Oreochromis niloticus
) fingerlings for 14 days to assess their biosafety. The results showed that the survival rates in all treated groups (98.75 ± 2.5 to 100.00 ± 0.0%) were not significantly different (
P
< 0.05) from the control group (commercial diet) (96.25 ± 2.5%), suggesting that isolates have no adverse effect on the host. This study revealed the presence of potential probiotic microorganisms in
E. eugeniae
that are beneficial to the aquaculture industry.
Abstract The third-stage larvae of Anisakis simplex sensu lato (s.l.) are found in many marine fishes. To ensure food safety, it is important to determine whether these larvae are present in the body ...muscle of commercial fish species. However, there is little information regarding the tissue specificity of Anisakis and two of its sibling species, A. simplex sensu stricto (s.s.) and Anisakis pegreffii , that are common in marine fish in Japanese waters. We orally challenged rainbow trout ( Oncorhynchus mykiss (Walbaum)), and olive flounder ( Paralichthys olivaceus (Temminck and Schlegel)) with L3 larvae of these two sibling species and monitored infection for 5 weeks. In rainbow trout, A. simplex s.s., but not A. pegreffii larvae, migrated into the body muscle. A small number of freely moving A. pegreffii larvae were recovered within the body cavity. In olive flounder, A. simplex s.s. larvae were found in both the body cavity and body muscle. A. pegreffii larvae were found only in the body cavity and primarily encapsulated in lumps. Our results indicate that there are differences in the sites of infection and host specificity between the two sibling species of A. simplex s.l.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Abstract Proper identification of Anisakis species infecting host fishes is very important to both human health and fish disease diagnosis. The foremost problem in the identification of Anisakis ...larvae in fishes is that L3 larvae cannot be easily differentiated morphologically, especially between A. simplex (sensu stricto) (s.s.) (Rudolphi, 1809) and A. pegreffii Campana-Rouget et Biocca, 1955. Instead, molecular means such as allozyme, mitochondrial DNA ( mt DNA) cox 2 region and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses had been successfully used. In this study, morphological differences of L3 larvae collected from fishes and in vitro -cultured L4 larvae and adult A. simplex (s.s.) and A. pegreffii were evaluated. Anisakis larvae were collected from 7 different host fishes within Japan. Undamaged A. simplex (s.s.) and A. pegreffii collected from Oncorhynchus keta (Walbaum) and Scomber japonicus Houttuyn, respectively, were used for in vitro -culture in order to obtain L4 and adult stages. Species identification was confirmed by PCR-RFLP analysis of the ITS region (ITS1-5.8S-ITS2) of ribosomal DNA and by mt DNA cox 2 gene sequencing. Results revealed that L3, L4 and adult stages of A. simplex (s.s.) and A. pegreffii are morphologically distinguishable based on ventriculus length, wherein the former has longer ventriculus (0.90–1.50 mm) than the latter (0.50–0.78 mm). For oesophagus/ventriculus ratio, these two species are distinguishable only during L4 and adult stages. Also, adult male A. simplex (s.s.) and A. pegreffii were found to be distinguishable by differences in the distribution pattern of the caudal papillae, particularly the 3rd pair of distal papillae.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Internet of Things (IoT) controlled a reconfigurable antenna with PIN diode switch for modern wireless communication is designed and implemented. Direct contact of biasing network with the antenna is ...eliminated and the switching unit is manipulated through IoT method. The proposed antenna has ring structures, in which the outer ring connects the inner ring structure through a PIN diode switch. The dimension of the proposed antenna is reported as 50 mm × 50 mm and its prototype has been made-up on epoxy-Fr4 substrate with 1.6 mm thickness. This antenna setup is made to reconfigurable in four bands (4.5 GHz, 3.5 GHz, 2.4 GHz, and 1.8 GHz) through switching provided by IoT device (NodeMCU). The antenna has a good return loss greater than -10dB.In switching state 2 the antenna has a return loss of -30 dB peak is attained at 3.4 GHz of operating frequency. Similarly the gain response of antenna is good in its operating bands of all switching states and obtained a maximum gain of 2.7 dB in 3.5 GHz. Bidirectional radiation pattern is obtained in all switching states of the antenna.
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