The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently creating a global health emergency. This crisis is driving a ...worldwide effort to develop effective vaccines, prophylactics, and therapeutics. Nucleic acid (NA)-based treatments hold great potential to combat outbreaks of coronaviruses (CoVs) due to their rapid development, high target specificity, and the capacity to increase druggability. Here, we review key anti-CoV NA-based technologies, including antisense oligonucleotides (ASOs), siRNAs, RNA-targeting clustered regularly interspaced short palindromic repeats-CRISPR-associated protein (CRISPR-Cas), and mRNA vaccines, and discuss improved delivery methods and combination therapies with other antiviral drugs.
Nucleic-acid based therapies are worth developing against coronavirus (CoV) outbreaks because of their high specificity and rapid development.Several key therapeutic nucleic acid (TNA) strategies, including antisense oligonucleotides (ASOs), siRNA, and RNA-targeting clustered regularly interspaced short palindromic repeats-CRISPR-associated protein (CRISPR-Cas) technologies targeting the viral genome are potentially applicable to combat CoVs.mRNA vaccines are attractive candidates to control CoV outbreaks with rapid/easy manufacturing, high potency, and safety.Optimal therapeutic cocktails comprising different TNAs or TNAs with other antiviral agents could act on multiple targets simultaneously, thereby enhancing antiviral effects and reducing risk of drug resistance.Lipid-ASO (LASO) nanomicelles enable cellular self-uptake and can be conjugated with antiviral molecules, providing promising anti-CoV combinational therapeutics that can be delivered directly to the lungs by aerosol.Advancement in chemical modifications and delivery vehicles remarkably enhance stability and pharmacokinetic profile of TNAs, facilitating clinical application of numerous TNAs; however, more studies on optimal chemical manufacturing and delivery methods improving safety and efficiency for TNAs are needed.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The rapid development of mRNA-based vaccines against the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) led to the design of accelerated vaccination schedules that have been extremely ...effective in naive individuals. While a two-dose immunization regimen with the BNT162b2 vaccine has been demonstrated to provide a 95% efficacy in naive individuals, the effects of the second vaccine dose in individuals who have previously recovered from natural SARS-CoV-2 infection has not been investigated in detail. In this study, we characterize SARS-CoV-2 spike-specific humoral and cellular immunity in naive and previously infected individuals during and after two doses of BNT162b2 vaccination. Our results demonstrate that, while the second dose increases both the humoral and cellular immunity in naive individuals, COVID-19 recovered individuals reach their peak of immunity after the first dose. These results suggests that a second dose, according to the current standard regimen of vaccination, may be not necessary in individuals previously infected with SARS-CoV-2.
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•SARS-CoV-2 antigen-specific CD4+CD154+ Th1 cells secrete IL-2 and IFN-γ•T cell-mediated IL-2 and IFNγ correlate with SARS-CoV-2 IgG humoral immunity•Naive subjects reach their peak of immunity after the second vaccine dose•COVID-19 recovered individuals reach their peak of immunity after the first vaccine dose
Scarce SARS-CoV-2 vaccine supplies are influencing vaccination policies in some countries. Lozano-Ojalvo et al. report that subjects with previous exposure to SARS-CoV-2 mount powerful immune responses after the first BNT162b2 vaccine dose, suggesting single vaccination regimens in COVID-19 recovered individuals.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
La investigación se originó en la Dirección Regional de Salud Huancavelica en la provincia de Churcampa que comprende una población de 32338 habitantes conforme al último reporte del Instituto ...Nacional de Estadística e Informática del 2021. En lo geográfico cruzando límites con las provincias de Tayacaja, Acobamba y el departamento de Ayacucho. Estos datos son de vital importancia pues permitieron coberturar el servicio de salud de forma integral y de acuerdo a las necesidades de la comunidad. La investigación destacó también la importancia de las gerencias subregionales, unidades ejecutoras en el sector Salud, Educación y Agricultura en el Perú.
Editorial Moraes, Joysi
Revista Pensamento Contemporâneo em Administração,
01/2023, Volume:
17, Issue:
1
Journal Article
Peer reviewed
Open access
Efeitos do treinamento em liderança em funcionarios de uma rede supermercadista: evidencias de um experimento de campo (Effects of leadership training on employees of a supermarket chain: evidence ...from a field experiment), de Neuza Lobato Rodrigues Vieira, Luciana Carvalho de Mesquita Ferreira, Samir Lotfi Vaz e Athos Carlos Silva, a partir de uma pesquisa junto a 100 gestores e 300 operadores em 10 das 23 lojas de um grupo lojista de faturamento na casa dos R$3 bilhöes, além de 750 consumidores, evidenciam que lojas que receberam treinamento nao apresentam desempenho superior quando comparadas as lojas que nao receberam. Sentidos do trabalho para maes trabalhadoras (Home queen? Acomodaçöes colaborativas: análise dos fatores determinantes na predisposiçao a reutilizaçao do serviço (Collaborative accomodations: analysis of the determinant factors in the predisposition to reuse the service), de José Lucas Silva Siqueira e Renata Francisco Baldanza, buscaram compreender as relaçoes entre determinantes do compartilhamento de acomodaçao e a predisposiçao a reutilizaçao desse serviço via Airbnb.
O processo de registro de marca no Brasil é realizado pelo Instituto Nacional de Propriedade Industrial (INPI). Muitos empresários não registram as suas marcas, muitas das vezes por não conhecerem ...como o processo funciona. A pandemia de covid-19 trouxe novas oportunidades de negócios, o que pode ter influenciado no pedido de registro da marca pelas empresas. Com o objetivo de realizar um mapeamento do registro de marcas no Brasil e os efeitos causados pela pandemia de covid-19, foi realizado um levantamento das informações contidas no site do INPI, na área “estatística e estudos econômicos”, nas seções “estatística” e “relatórios” ao longo de 2014 a 2021. Os resultados mostraram que o número de pedido de registro de marca no INPI cresceu ao longo dos anos de 2014 a 2021, sendo impulsionado durante a pandemia. Conclui-se que o pedido de registro de marca e sua concessão no Brasil está em crescimento e que a pandemia de covid-19 não afetou o pedido de registro de marca dos residentes, porém afetou dos não residentes. Entretanto, ainda há muitos pedidos não concedidos, o que pode ser reflexo da falta de informação e acompanhamento do processo de pedido de registro de marca.
Nacida en Venezuela, Nydia Gutiérrez es Curadora independiente con base en Bogotá y Boston. Arquitecta y museóloga por la Universidad Javeriana de Bogotá y la JFK University de San Francisco. ...Profesora de Curaduría en la Maestría en Museología de la Universidad Nacional. Realizó el Balance del Programa Salones de Artistas de Mincultura. Fue curadora jefe del Museo de Antioquia (Medellín,Colombia) y jefe de curadores del Museo de Bellas Artes de Caracas. Obtuvo una Beca de Investigación de Mincultura para escribir una de las Monografias de la serie Artistas Colombianos y ha escrito numerosos textos de catálogos y otras publicaciones sobre arte
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