Highlights • A hyperglycemic hormone cDNA was characterized in two peracarid crustaceans. • CHH was localized in the cerebral ganglia and showed striking similarities between species. • Each species ...exhibited robust, endogenous circatidal or circadian activity rhythms. • Temporal expression of CHH mRNA was neither circadian nor circatidal.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
This article analyzed the mission, work and role of Eurydice, and expounded its inheritance and manifestation of Jullien’s comparative education research sprit by literature analysis and historical ...research methods. As an official educational research institution of the European Union, Eurydice can be considered as inheriting from the initiative and spirit of Jullien’s comparative education research. The research purpose, research methods and scope of comparative education initiated by Jullien in the “Plan and Preliminary Views for a Work on Comparative Education” two hundred years ago was demonstrated and developed in Eurydice’s research work and achievements. Regarding the research purpose of comparative education, Eurydice aims to do educational analysis for policymaking, which is the purpose of educational reform initiated by Jullien. As for the research methods of comparative education, Eurydice establishes educational indicators and databases, demonstrating Jullien’s appeal for educational science. With
Osip Mandel'shtam's poetry has been interpreted abundantly by scholars and it might be seen as preposterous to return to one of the most discussed poems in general: “Chut' mertsaet prizrachnaia ...stsena” dealing with Gluck's opera Orpheus and Eurydice. My approach will be an intermedial one and I will analyse it in relation to the famous productions at the Mariinskii Opera in 1910 and in 1919. The task will be to scrutinize the relationship between the text of the poem and various paratexts, or, in the words of Derrida (1987) and broader, the relation between ergon and parergon. The focus is on the aesthetic impact of these relations more than on the question of genesis or influence. The basis for the interpretation will be the opposition between the period of the performance and the post-performance time, as well as the notion of liminality. The poem will be analysed against the background of the motif of the exit from the theatre in Russian 19th century Russian culture both in relation to literature and to a lithography from 1843 made by Rudol'f Zhukovskii. I will, however, use in particular material concerning the opera performance alluded to in the poem.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
is an amphipod crustacean that inhabits the supralittoral zone on sandy beaches in the Northeast Atlantic and Mediterranean.
exhibits endogenous locomotor activity rhythms and time-compensated sun ...and moon orientation, both of which necessitate at least one chronometric mechanism. Whilst their behaviour is well studied, currently there are no descriptions of the underlying molecular components of a biological clock in this animal, and very few in other crustacean species.
We harvested brain tissue from animals expressing robust circadian activity rhythms and used homology cloning and Illumina RNAseq approaches to sequence and identify the core circadian clock and clock-related genes in these samples. We assessed the temporal expression of these genes in time-course samples from rhythmic animals using RNAseq.
We identified a comprehensive suite of circadian clock gene homologues in
including the 'core' clock genes
(
),
(
),
(
),
(
), and
(
). In addition we describe the sequence and putative structures of 23 clock-associated genes including two unusual, extended isoforms of pigment dispersing hormone (
). We examined time-course RNAseq expression data, derived from tissues harvested from behaviourally rhythmic animals, to reveal rhythmic expression of these genes with approximately circadian period in
and
. Of the clock-related genes,
(
,
(
),
(
),
(
),
(
),
(
),
(
), sirt7 (
) and supernumerary limbs (
) show temporal changes in expression.
We report the sequences of principle genes that comprise the circadian clock of
and highlight the conserved structural and functional domains of their deduced cognate proteins. Our sequencing data contribute to the growing inventory of described comparative clocks. Expression profiling of the identified clock genes illuminates tantalising targets for experimental manipulation to elucidate the molecular and cellular control of clock-driven phenotypes in this crustacean.
Abstract The development of expressed sequence tags (ESTs) for crustacean cDNA libraries and their deposition in publicly accessible databases has generated a rich resource for peptide discovery in ...this commercially and ecologically important arthropod subphylum. Here, we have conducted in silico searches of these databases for unannotated ESTs encoding putative neuropeptide precursors using the BLAST program tblastn, and have predicted the mature forms of the peptides encoded by them. The primary strategy used was to query the database with known decapod prepro-hormone sequences or, in some instances, insect precursor protein sequences. For neuropeptides for which no prepro-hormones are known, the peptides themselves were used as queries. For those peptides expected to originate from a common precursor, the individual sequences were combined, with each peptide flanked by a dibasic processing site and, if amidated, a glycine residue. Using these approaches, 13 unannotated ESTs encoding putative neuropeptide precursors were found. For example, using the first strategy, putative Marsupenaeus japonicus prepro-hormones encoding B-type allatostatins, neuropeptide F (NPF), and orcokinins were identified. Similarly, several Homarus americanus ESTs encoding putative orcokinin precursors were found. In addition to the decapod prepro-hormones, ESTs putatively encoding a NPF isoform and a red pigment concentrating hormone-like peptide were identified from the cladoceran Daphnia magna , as was one EST putatively encoding multiple tachykinin-related peptides from the isopod Eurydice pulchra . Using the second strategy, we identified a Carcinus maenas EST encoding HIGSLYRamide, a peptide recently discovered via mass spectrometry from Cancer productus. Using mass spectral methods we confirmed that this peptide is also present in Carcinus maenas . Collectively over 50 novel crustacean peptides were predicted from the identified ESTs, providing a strong foundation for future investigations of the evolution, regulation and function of these and related molecules in this arthropod taxon.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
In the current study, the macroinfauna communities inhabiting 12 sandy beaches along the Spanish coast of the Gulf of Cadiz were describe in order to characterize their unexplored biodiversity, their ...zonation patterns and their environmental features. A total of 66 macroinfauna taxa were recorded on 12 sandy beaches, ranging from 4 to 33 species. Density reached 848 individual per m super(2). The individual zonation pattern ranged from two to three zones, regardless of the morphodynamic state. A common zonation pattern of the whole set of beaches was established, comprising three across-shore biological zones. Generally, the supralittoral zone was typified by the air-breathing amphipod Talitrus saltator and coleopterans; the middle zone was dominated by true intertidal species, such as Haustoriidae amphipods (Haustorius arenarius), Cirolanidae isopods (Eurydice affinis), Spionidae polychaetes (Scolelepis spp.) and nemerteans; and the lower or sublittoral zone was typified by Pontoporeiidae amphipods, mysids and spionid polychaetes. Sediment moisture, average grain size, organic matter content and elevation were the main predictor variables of zonation patterns. We used the conservation and recreation potential indices to show how this information can be useful for coastal management. Most beaches studied show a score above 5 on the conservation index, so the beaches have a reasonable potential for conservation management.Original Abstract: En el presente estudio. se describen las comunidades de macroinfauna de doce playas arenosas de la costa espanola del Golfo de Cadiz con el objetivo de caracterizar su biodiversidad, sus patrones de zonacion, y sus caracteristicas ambientales. Un total de 66 taxones de macroinfauna fueron registrados en las playas de estudio, oscilando entre 4 y 33 especies, mientras que la densidad total fue de 848 individuos por m super(2). El patron de zonacion individual vario de dos a tres zonas, independientemente del estado morfodinamico de la playa. Se establecio un patron de zonacion comun de todo el conjunto de playas, formado por tres zonas biologicas. En general, la zona supralitoral fue tipificada por el anfipodo Talitrus saltator y diversas familias de coleopteros. La zona media fue dominada por verdaderas especies intermareales, como anfipodos de la famila Haustoriidae (Haustorius arenarius), isopodos cirolanidos (Eurydice affinis), poliquetos (Scolelepis spp.) y nemertinos. La zona sublitoral se caracterizo por anfipodos de la familia Pontoporeiidae, misidaceos y poliquetos espionidos. La humedad del sedimento, el tamano medio de grano, el contenido en materia organica y la elevation fueron las principales variables influyentes en los patrones de zonacion. Por ultimo, toda esta informacion fue integrada para la aplicacion de los indices de Conservacion y Recreation, para mostrar como esta informacion puede ser util para la gestion costera. La mayoria de las playas mostraron una puntuacion superior a 5 en el Indice de Conservacion. indicando un razonable potential para la conservacion.
L’épigramme en distiques élégiaques citée par Plutarque (Sur l’éducation des enfants, 20 Moralia, 14 B9-C3) a longtemps été une source majeure sur la mère de Philippe II de Macédoine. La ...transmission imparfaite du texte a suscité diverses propositions pour l’amender, qui ne sont pas entièrement satisfaisantes. Est proposée ici une correction plus fidèle aux textes des manuscrits et qui a l’avantage de mieux correspondre aux usages des dédicaces grecques.
For a long time the elegiac epigramm known by Plutarch (On the Education of Children, 20 Moralia, 14 B9-C3) was a most important source on Philipp II’s mother. The imperfect transmission of the text caused its different emendations, not all happy. Here is proposed a solution nearer to the manuscripts and more in conformity with the Greek ways of dedicating.
The Norway lobster, Nephrops norvegicus, is a burrowing decapod with a rhythmic burrow emergence (24 h) governed by the circadian system. It is an important resource for European fisheries and its ...behavior deeply affects its availability. The current knowledge of Nephrops circadian biology is phenomenological as it is currently the case for almost all crustaceans. In attempt to elucidate the putative molecular mechanisms underlying circadian gene regulation in Nephrops, we used a transcriptomics approach on cDNA extracted from the eyestalk, a structure playing a crucial role in controlling behavior of decapods. We studied 14 male lobsters under 12-12 light-darkness blue light cycle. We used the Hiseq 2000 Illumina platform to sequence two eyestalk libraries (under light and darkness conditions) obtaining about 90 millions 100-bp paired-end reads. Trinity was used for the de novo reconstruction of transcriptomes; the size at which half of all assembled bases reside in contigs (N50) was equal to 1796 (light) and 2055 (darkness). We found a list of candidate clock genes and focused our attention on canonical ones: timeless, period, clock and bmal1. The cloning of assembled fragments validated Trinity outputs. The putative Nephrops clock genes showed high levels of identity (blastx on NCBI) with known crustacean clock gene homologs such as Eurydice pulchra (period: 47%, timeless: 59%, bmal1: 79%) and Macrobrachium rosenbergii (clock: 100%). We also found a vertebrate-like cryptochrome 2. RT-qPCR showed that only timeless had a robust diel pattern of expression. Our data are in accordance with the current knowledge of the crustacean circadian clock, reinforcing the idea that the molecular clockwork of this group shows some differences with the established model in Drosophila melanogaster.
Full text
Available for:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
PDF
