Robenacoxib (RX) is a non-steroidal anti-inflammatory drug (NSAID) of the coxib class. This study aimed to evaluate the plasma dispositions and faecal excretion profiles of RX in Alpine and Saanen ...goats following oral and subcutaneous routes. Two different goat breeds were allocated into two treatment groups concerning the breed. RX was administered subcutaneously to animals at a dose of 4 mg/kg b.w. Following a one-week washout period, RX was administered by oral route to the same animals at the same dose. Heparinized blood samples were collected from all animals before drug administration (0 h) and subsequently up to 24 h. Faecal samples were collected at various times between 8 h and 36 h. The concentrations of RX in plasma and faeces were determined by HPLC. The plasma half-life (T1/2λz) of RX in Saanen goats (1.21 h) was significantly longer (P < 0.017) than in Alpine goats (0.90 h) after subcutaneous administration. In both goat breeds, statistical differences were observed between subcutaneous and oral administration of RX for T1/2λz, Tlast, Cmax, AUC0-∞, and MRT0-∞. Faecal Cmax and Tmax parameters following oral administrations were 0.92 µg/g and 0.85 µg/g at 30 h and at 24 h in Alpine and Saanen goats, respectively. The difference in plasma protein ratio between Alpine and Saanen goats may have affected the T1/2λz of the drug. NSAIDs are among the drug groups frequently detected in aquatic and terrestrial ecosystems around the world and there are data on the effects of NSAID residues on wildlife and aquatic species. Therefore, revealing the excretion of NSAIDs, which are frequently used in the veterinary field, in faeces and urine should be considered for ecological sustainability.
•Plasma dispositions and faecal excretion of RX in Saanen and Alpine goats after PO and SC administration were evaluated.•Plasma T1/2λz of RX after SC administration was affected by goat breeds.•Faecal concentration of RX after PO administration was similar in both breeds.•RX concentration in faeces was 1.73 and 1.77 times higher than plasma in Alpine and Saanen goats, respectively.•SC administration of RX in goats may be a preferred route, particularly in acute cases.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Yellowtail kingfish (Seriola lalandi) is a fast-growing fish species. One of the challenges of farming yellowtail kingfish in recirculating aquaculture systems is their poor faeces integrity, also ...referred to as ‘diarrhoea-like’ faeces. Whether diarrhoea-like faeces occur under conditions when feeding on its natural feed items or are diet induced, is unclear. This study assessed the effect of feed type (pelleted vs. natural feed) on the faecal characteristics and faecal waste production of yellowtail kingfish. Three dietary treatments were studied over a 35-d experimental period: a pelleted diet based on marine ingredients (Marine; open formula); an experimental pelleted feed based on the ingredient composition of a commercial kingfish feed (Commercial Dummy, CD; closed formula); and a diet composed of four, individually fed raw (unprocessed) natural ingredients and commercial dummy pellets at a ratio of 1:1 on dry matter basis (Natural and Commercial Dummy, NCD). The NCD treatment was intended to clarify whether diarrhoea-like faeces are naturally occurring in yellowtail kingfish. Each dietary treatment was tested in four tanks, which were stocked with 27 yellowtail kingfish (mean initial weight 39 g). Fish were fed to apparent satiation twice daily for 1 h. For each tank nutrient digestibility and faecal characteristics were measured. The inclusion of natural ingredients reduced the faecal waste production (p < 0.001). Furthermore, the faeces integrity of yellowtail kingfish fed with natural ingredients was not poor (not diarrhoea-like). At the natural treatment, fish excreted faecal pellets and short strings, which were not observed at the other treatments. Faecal waste collected from fish receiving only pelleted feed was classified as diarrhoea-like. The highest faeces removal efficiency by settling was observed at the NCD treatment compared to the other treatments (p < 0.001). Consequently, the lowest amount of non-removed faeces per feed intake (p < 0.001) was observed at the NCD treatment (62.9 g OM/kg OM FI), followed by the Marine (101.1 g OM/kg OM FI) and Commercial Dummy treatment (111.7 g OM/kg OM FI). In conclusion, this study shows the potential of dietary interventions to alter the amount and integrity of faecal waste. This offers possibilities to reduce the total suspended solid load for yellowtail kingfish farming in recirculating aquaculture systems.
•Poor faeces integrity is not naturally occurring in yellowtail kingfish.•Pelleted diets introduce a poor faeces integrity in yellowtail kingfish.•Natural feed ingredient inclusion improved nutrient digestibility.•Dietary intervention can alter the integrity of faecal waste in yellowtail kingfish.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
SARS-CoV-2, the virus that causes COVID-19, has been found in the faeces of infected patients in numerous studies. Stool may remain positive for SARS-CoV-2, even when the respiratory tract becomes ...negative, and the interaction with the gastrointestinal tract poses a series of questions about wastewater and its treatments. This review aims to understand the viral load of SARS-CoV-2 in faeces and sewage and its fate in wastewater treatment plants (WWTPs).
The viral load in the faeces of persons testing positive for SARS-CoV-2 was estimated at between 5·103 to 107.6 copies/mL, depending on the infection course. In the sewerage, faeces undergo dilution and viral load decreases considerably in the wastewater entering a WWTP with a range from 2 copies/100 mL to 3·103 copies/mL, depending on the level of the epidemic. Monitoring of SARS-CoV-2 in sewage, although no evidence of COVID-19 transmission has been found via this route, could be advantageously exploited as an early warning of outbreaks. Preliminary studies on WBE seem promising; but high uncertainty of viral loads in wastewater and faeces remains, and further research is needed.
The detection of SARS-CoV-2 in sewage, based on RNA sequences and RT-PCR, requires a shared approach on sample pre-treatment and on-site collection to ensure comparable results. The finding of viral RNA in stools does not imply that the virus is viable and infectious. Viability of CoVs such as SARS-CoV-2 decreases in wastewater - due to temperature, pH, solids, micropollutants - but high inactivation in WWTPs can be obtained only by using disinfection (free chlorine, UVC light). A reduction in the quantity of disinfectants can be obtained by implementing Membrane-Bioreactors with ultrafiltration to separate SARS-CoV-2 virions with a size of 60–140 nm. In sludge treatment, thermophilic digestion is effective, based on the general consensus that CoVs are highly sensitive to increased temperatures.
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•The route of SARS-CoV-2 from faeces to wastewater treatment plants is analysed.•Viral load in the faeces of positive people for SARS-CoV-2 is 5·103–107.6 copies/mL.•Viral load decreases from 2 copies/100 mL to 3·103 copies/mL when entering a WWTP.•For WBE high uncertainty of viral loads remains, and further research is needed.•CoVs inactivation in WWTPs is enhanced by tertiary treatments and disinfection.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The study aimed to explore the connection between horses’ total tract apparent dry matter digestibility (ttaDMD) of various roughages and faecal particle size (FPS), to develop a tool to rank ...roughage digestibility. Six horses, varying in size and breed, were fed six distinct roughages (four cuts of fescue, perennial ryegrass/clover mix, and straw). Each feeding period lasted 16 days, with a 14-day adaptation phase. Faecal samples underwent wet sieving analysis. Roughage chemical composition was evaluated using NIRs. Faecal and roughage acid insoluble ash content were used to estimate ttaDMD and FPS was determined by wet sieving using a set of 6 sieves of different mesh sizes (S1 to S6; 6.50, 4.50, 2.36, 1.18, 0.6 and 0.15 mm respectively). The effects of roughage ttaDMD on FPS across sieves were analysed using GLM. Linear regressions and correlations were performed between faecal residues on each sieve and mesh sizes of the sieves to create a mathematical tool to rank ttaDMD based on FPS distribution. Increased ttaDMD was associated with larger FPS, with perennial ryegrass/clover mix (ttaDMD 74.44%) and straw (ttaDMD 44.57%) resulting in smaller and larger faecal particles respectively. Correlations were observed between ttaDMD, S1 and S2 (r = 0.639 and 0.668; p ≤ 0.001). The angular coefficient of the linear function between faecal residues on each sieve support the idea to estimate roughages digestibility from FPS (R2 = 0.4153). Consequently, determining faecal residues across sieves of varying mesh sizes could serve as an indirect method for ranking roughages digestibility.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Adherence to the guidelines of The International Organization for Standardization (ISO) for the isolation of Salmonella from faeces is important to laboratory personnel, researchers and ...epidemiologists to ensure effective diagnosis, maximal recovery and high sensitivity/specificity of laboratory tests. The aim of this study is to review the ISO guidelines for the detection of Salmonella from faeces using standards published by ISO and other internationally recognized bodies. The ISO 6579 of 2002 described the process for the detection of Salmonella in faeces. About 25g of faecal samples is collected, mixed together and specimen taken from different parts to ensure maximum recovery of the organism. Processes for the identification of the organism were prescribed by the standard. In the amendment made to ISO 6579 (Annex D) it was recommended that Modified Semisolid Rappaport Vasiliadis (MSRV) be used as selective enrichment medium. Further amendment made to this standard was in 2007 where clause 4 described non-selective pre-enrichment, selective enrichment, use of 2 selective solid media and biochemical tests as the 4 stages of the isolation of Salmonella in faeces. The amendment of ISO 6579 in 2017 combined the guidelines for the isolation of Salmonella species, S. Typhi/Paratyphi from milk/milk-products and faeces. It proposed the use of selenite cysteine broth in addition to Rappaport Vasiliadis (RVS) and Muller-Kauffmann Tetrathionate-Novobiocin (MkTTn) and BSA in addition to Xylose Lysine Deoxycholate (XLD) Agar for the isolation of S. typhi and paratyphi. It recommended the performance of methyl-red, Simmon-citrate, H2S, motility and urease biochemical tests, deleted Vogues Proskaer test while indole and β-Galactosidase (ONPG) tests were made optional. This standard has evolved over the years to ensure efficient recovery of Salmonella from faeces based on real-time result and feedback received by the organization from laboratories all over the world.
Wastewater-based epidemiology (WBE) is one of the most promising approaches to effectively monitor the spread of COVID-19. The virus concentration in faeces and its temporal variations are essential ...information for WBE. While some clinical studies have reported SARS-CoV-2 concentrations in faeces, the value varies amongst patients and changes over time. The present study aimed to examine how the temporal variations in the concentration of virus in faeces affect the monitoring of disease incidence. We reanalysed the experimental findings of clinical studies to estimate the duration of virus shedding and the faecal virus concentration. Available experimental data as of 23 October 2020 were collected. The viral shedding kinetics was modelled, and the dynamic model was fitted to the collected data by a Bayesian framework. Using posterior distributions, the duration of viral shedding and the concentration of virus copies in faeces over time were computed. We estimated the median concentration of SARS-CoV-2 in faeces as 3.4 (95% CrI: 0.24–6.5) log copies per gram-faeces over the shedding period, and our model implied that the duration of viral shedding was 26.0 days (95% CrI: 21.7–34.9), given the current standard quantification limit (Ct = 40). With simulated incidences, our results also indicated that a one-week delay between symptom onset and wastewater sampling increased the estimation of incidence by a factor of 17.2 (i.e., 101.24 times higher). Our results demonstrated that the temporal variation in virus concentration in faeces affects microbial monitoring systems such as WBE. The present study also implied the need for adjusting the estimates of virus concentration in faeces by incorporating the kinetics of unobserved concentrations. The method used in this study is easily implemented in further simulations; therefore, the results of this study might contribute to enhancing disease surveillance and risk assessments that require quantities of virus to be excreted into the environment.
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•SARS-CoV-2 is excreted in faeces, but its time course has been understudied.•SARS-CoV-2 concentration in faeces re-analysed using a kinetic viral shedding model•The duration of viral shedding was estimated to be 20–32 days.•Median concentration was 2.6 log copies/g of faeces over the shedding period.•WBE needs to consider temporal variations in viral concentrations in faeces.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The sustainability of commercial aquaculture production depends critically on prioritizing fish welfare management. Besides monitoring welfare parameters such as fish behaviour and water quality, ...fish stress level can also provide a reliable measure of the welfare status of farmed fish. Cortisol and 5 of its metabolites (5β-THF, cortisone, 5β-DHE, 5β-THE, β-cortolone) were previously identified by the authors as suitable stress biomarkers of farmed Atlantic salmon. Based on this knowledge, the present study aimed to investigate the time-related dynamics of these metabolites in plasma, skin mucus, bile and faeces over a 72 h- period. The objective was to determine the optimal sampling time for each matrix and to understand the clearance pathway of these metabolites following stress. An experiment was carried out using a total of 90 Atlantic salmon with an average weight of 438 (±132) g. The average sea temperature was 6.9 °C during the experimental period. A control group of 10 fish was first collected before the remaining 80 fish were submitted to a stress of netting and subsequent relocation into two separate cages. From each of these two stress groups, 10 fish were sampled at 1 h, 2 h, 4 h, 6 h and 12 h, 24 h, 48 h, 72 h after the stress event respectively. The concentrations of cortisol and its metabolites were measured at each of the sampling timepoint. The results demonstrated that plasma cortisol metabolites reached the highest concentration 4 h after stress and remained elevated despite the slight decrease for the remaining timepoints. The peak level was observed at 12 h post-stress in skin mucus and 24 h in bile and faeces. The findings suggest that these timepoints are the optimal for sampling Atlantic salmon post-smolt following stressful events in acute stress studies. Furthermore, the results reveal that analysing cortisol and its metabolites, both in free and conjugated forms, rather than free cortisol provides greater flexibility as their concentrations are less affected by sampling procedure. This study confirms the appropriateness of skin mucus and faeces as less-invasive sample matrices for fish stress evaluation and provides a basis for further developing low invasive tools for monitoring the welfare of farmed salmonid.
•Measuring cortisol metabolite level is a reliable method for assessing fish stress.•Sampling time post-stress is dependent on the matrix used for analysis.•Skin mucus and faeces are low-invasive matrices for analysing fish stress response.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Strain CBA3108
T
is a Gram-positive, non-spore-forming, obligately anaerobic bacterium isolated from horse faecal samples obtained in Jeju Island, Republic of Korea. The cells of CBA3108
T
are ...non-motile short rods that have been assessed as catalase-positive and oxidase-negative. Growth of the strain occurs under the following conditions: 25–45 °C (optimum, 35 °C); pH 6–9 (optimum, pH 6); and in the presence of 0–6 % (w/v) NaCl (optimum, 2%). Major fatty acids in the strain include C
15 : 0
iso and C
15 : 0
iso DMA, while major polar lipids include phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain CBA3108
T
forms a phyletic lineage distinct from other closely related species within the genus
Cutibacterium
. It was found to be most closely related to
Cutibacterium avidum
ATCC 25577
T
(98.27 % 16S rRNA gene sequence similarity) and other strains within the genus (≤98.0 %). The genomic DNA G+C content of strain CBA3108
T
was 63.2 mol%. The
in silico
DNA–DNA hybridization values of strain CBA3108
T
with
C. avidum
ATCC 25577
T
,
C. porci
WCA-380-WT-3A
T
and
C. acnes
subsp.
acnes
DSM 1897
T
were 33.6, 21.7 and 22.7 %, respectively. Its phenotypic, chemotaxonomic and molecular properties support the hypothesis that strain CBA3108
T
represents a novel species in the genus
Cutibacterium
, for which we propose the name
Cutibacterium equinum
sp. nov. The type strain is CBA3108
T
(=KACC 22889
T
=JCM 35966
T
).
•Antibiotics cause profound changes in the intestinal microbiota. These changes including a decrease in bacterial diversity, changes in the abundances of certain bacteria and an increase in ...antibiotic resistance.•The longest duration of changes was observed after treatment with ciprofloxacin (one year), clindamycin (two years) and clarithromycin plus metronidazole (four years). However, these findings are limited by follow-up times.•Understanding the effects of antibiotics on the intestinal microbiota will help tailor antibiotic treatment to minimise this ‘collateral damage’.
Antibiotics change the composition of the intestinal microbiota. The magnitude of the effect of antibiotics on the microbiota and whether the effects are short-term or persist long-term remain uncertain. In this review, we summarise studies that have investigated the effect of antibiotics on the composition of the human intestinal microbiota.
A systematic search was done to identify original studies that have investigated the effect of systemic antibiotics on the intestinal microbiota in humans.
We identified 129 studies investigating 2076 participants and 301 controls. Many studies reported a decrease in bacterial diversity with antibiotic treatment. Penicillin only had minor effects on the intestinal microbiota. Amoxicillin, amoxcillin/clavulanate, cephalosporins, lipopolyglycopeptides, macrolides, ketolides, clindamycin, tigecycline, quinolones and fosfomycin all increased abundance of Enterobacteriaea other than E. coli (mainly Citrobacter spp., Enterobacter spp. and Klebsiella spp.). Amoxcillin, cephalosporins, macrolides, clindamycin, quinolones and sulphonamides decreased abundance of E. coli, while amoxcillin/clavulante, in contrast to other penicillins, increased abundance of E. coli. Amoxicllin, piperacillin and ticarcillin, cephalosporins (except fifth generation cephalosporins), carbapenems and lipoglycopeptides were associated with increased abundance of Enterococcus spp., while macrolides and doxycycline decreased its abundance. Piperacillin and ticarcillin, carbapenems, macrolides, clindamycin and quinolones strongly decreased the abundance of anaerobic bacteria. In the studies that investigated persistence, the longest duration of changes was reported after treatment with ciprofloxacin (one year), clindamycin (two years) and clarithromycin plus metronidazole (four years). Many antibiotics were associated with a decrease in butyrate or butryrate-producing bacteria.
Antibiotics have profound and sometimes persisting effects on the intestinal microbiota, characterised by diminished abundance of beneficial commensals and increased abundance of potentially detrimental microorganisms. Understanding these effects will help tailor antibiotic treatment and the use of probiotics to minimise this ‘collateral damage’.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
This paper comprises an updated version of the 2014 review which reported 1846 volatile organic compounds (VOCs) identified from healthy humans. In total over 900 additional VOCs have been reported ...since the 2014 review and the VOCs from semen have been added. The numbers of VOCs found in breath and the other bodily fluids are: blood 379, breath 1488, faeces 443, milk 290, saliva 549, semen 196, skin 623 and urine 444. Compounds were assigned CAS registry numbers and named according to a common convention where possible. The compounds have been included in a single table with the source reference(s) for each VOC, an update on our 2014 paper. VOCs have also been grouped into tables according to their chemical class or functionality to permit easy comparison. Careful use of the database is needed, as a number of the identified VOCs only have level 2-putative assignment, and only a small fraction of the reported VOCs have been validated by standards. Some clear differences are observed, for instance, a lack of esters in urine with a high number in faeces and breath. However, the lack of compounds from matrices such a semen and milk compared to breath for example could be due to the techniques used or reflect the intensity of effort e.g. there are few publications on VOCs from milk and semen compared to a large number for breath. The large number of volatiles reported from skin is partly due to the methodologies used, e.g. by collecting skin sebum (with dissolved VOCs and semi VOCs) onto glass beads or cotton pads and then heating to a high temperature to desorb VOCs. All compounds have been included as reported (unless there was a clear discrepancy between name and chemical structure), but there may be some mistaken assignations arising from the original publications, particularly for isomers. It is the authors' intention that this work will not only be a useful database of VOCs listed in the literature but will stimulate further study of VOCs from healthy individuals; for example more work is required to confirm the identification of these VOCs adhering to the principles outlined in the metabolomics standards initiative. Establishing a list of volatiles emanating from healthy individuals and increased understanding of VOC metabolic pathways is an important step for differentiating between diseases using VOCs.
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