For the first time, in the conditions of the Magadan region, have been carried out studies on the use of a feed additive (FA) based on mountain pine in combination with lichens in the rations of ...young cattle (C) of Holstein breed and cross-bred young of Holstein and Aberdeen Angus breeds of the dairy and growing period. The use of FA positively affects the growth rate, daily average growth, blood counts, young growth resistance and digestibility of feed. The live-weight of the experimental gobies of Holstein breed at the age of 16 months, which additionally received the FA ration, exceeded the rate of the control group up to 2.15 kg (0.58%), the crossbreeds of the Aberdeen-Angus breed exceeded the rate of a control group up to 9.55 kg (2.3%) (P≤0.05). The relative growth rate (according to S. Brody) of gobies at the age of 16 months, which got FA was higher than of gobies of the control groups. The growth rate of experimental gobies of Holstein breed is 0.12% higher, and of crossbred gobies is 2.57% higher respectively. A study of the hematological composition of the blood of experimental half-blood gobies of the Aberdeen-Angus breed showed that relative to the control group, the concentration of eosinophils increases by 0.4%, monocytes 0.8%, lymphocytes by 7.2%, the concentration of band neutrophil decreases by 1%, segmented neutrophils by 7.4%. The young of the experimental groups had better digestibility of dietary nutrients and feed costs per 1 kg of growth in comparison with the control groups.
The genus Rhinogobius was widely distributed in East Asia. In the present study, the complete mitochondrial genome of Rhinogobius sp., possible a new species of freshwater goby from Anhui province of ...China, was sequenced for the first time. Sequence analysis showed that it is 16,511 bp in length with A + T content of 52.3%, consisting of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and a control region (CR). Phylogenetic analyses placed Rhinogobius sp. in a well-supported monophyletic cluster with other Rhinogobius fish and the phylogenetic position of Rhinogobius sp. was closer to Rhinogobius cliffordpopei.
Host-parasite systems provide an ideal platform to study evolution at different levels, including codivergence in a historical biogeography context. In this study we aim to describe biogeographic and ...codivergent patterns and associated processes of the Goodeinae freshwater fish and their digenean parasite (Margotrema spp.) over the last 6.5 Ma (million years), identifying the main factors (host and/or hydrogeomorphology) that influenced the evolution of Margotrema. We obtained a species tree for Margotrema spp. using DNA sequence data from mitochondrial and nuclear molecular markers (COI and ITS1, respectively) and performed molecular dating to discern divergence events within the genus. The dispersal-extinction-cladogenesis (DEC) model was used to describe the historical biogeography of digeneans and applied to cophylogenetic analyses of Margotrema and their goodeine hosts. Our results showed that the evolutionary history of Margotrema has been shaped in close association with its geographic context, especially with the geological history of central Mexico during the Pleistocene. Host-specificity has been established at three levels of historical association: a) Species-Species, represented by Xenotaenia resolanae-M. resolanae exclusively found in the Cuzalapa River Basin; b) Species-Lineage, represented by Characodon audax-M. bravoae Lineage II, exclusive to the Upper and Middle Mezquital River Basin, and c) Tribe-Lineage, including two instances of historical associations among parasites and hosts at the taxonomical level of tribe, one represented by Ilyodontini-M. bravoae Lineage I (distributed across the Ayuquila and Balsas River Basins), and another comprised of Girardinichthyini/Chapalichthyini-M. bravoae Lineage III, found only in the Lerma River Basin. We show that the evolutionary history of the parasites is, on several occasions, in agreement with the phylogenetic and biogeographic history of their hosts. A series of biogeographic and host-parasite events explain the codivergence patterns observed, in which cospeciation and colonisation via host-switching and vicariant plus dispersal events are appreciated, at different times during the diversification history of both associates, particularly during the Pleistocene.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Gobies (Gobiidae sensu Gill & Mooi, 2012) are one of the most diverse families of vertebrates, and comprise over 1700 species of marine, brackish and freshwater fishes. Phylogenetic studies based on ...morphological characters and mtDNA have suggested that goby diversity is asymmetrically split between a speciose clade of predominantly marine species, and a less rich, but ecologically diverse, clade comprising predominantly freshwater and brackish species. This study is the first to explore this deep divide in gobies and their relationships at the family level using phylogenetic data from nuclear genes (RAG1, rhodopsin). Our results confirm the split within the Gobiidae, and agree with prior molecular studies on the inclusion of the following taxa within the two goby clades: (i) the more diverse of the two clades of gobies (the ‘Gobiidae’ sensu stricto of Thacker 2009) comprises the gobiines, microdesmines, ptereleotrines and kraemeriines; (ii) the less diverse of the two gobiid clades (‘Gobionellidae’ sensu Thacker 2009) includes the gobionellines, oxudercines, amblyopines, sicydiines, as well as the European sand gobies. Some relationships within the two major gobiid clades remain unclear. Specifically, there remains confusion regarding the monophyly and interrelationships between the northern Pacific gobionellines, the Mugilogobius group gobionellines, and the European sand gobies. Additionally, within Thacker's (2009) Gobiidae sensu stricto , there are several well-supported groups (e.g. the wormfishes and dartfishes, the Coral Gobies, the Gobiosomatini), yet relationships among these groups are still poorly resolved despite the use of data from two conserved nuclear genes. Future phylogenetic analyses of gobies will benefit greatly from taxon sampling that includes groups that have been historically under-represented in molecular studies (e.g. European sand gobies, northern Pacific gobionellines, African species), as well as deeper genetic sampling including large numbers of independent loci from throughout the genome (i.e. a phylogenomic approach).
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BFBNIB, GIS, IJS, IZUM, KILJ, KISLJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
The results of a study of the fauna of multicellular parasites of the Caspian bighead goby
Neogobius iljini
(Vasiljeva et Vasiljev, 1996) in three reaches of the Kuibyshev Reservoir (Middle Volga) ...are presented. Twelve species and undefined forms of parasites were found, including a specific to the fam. Gobiidae metacercaria
Holostephanus cobitidis
. The most diverse fauna of macroparasites is observed in the lower reaches of the reservoir (Priplotinny reach). The dominant species in the parasite fauna of the Caspian goby of the studied reservoir is the alien trematode
Nicolla skrjabini
, whose natural range is limited to the rivers of the Azov and Black seas basin.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
We applied next-generation sequencing (NGS) method to construct the complete mitochondrial genome of Glossogobius aureus. The obtained mitogenome of G. aureus (16,590 bp) exhibited a typical ...structure harboring 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one control regions (D-loop). Most of mitochondrial genes are encoded on the heavy (H) strand, except for eight tRNAs and ND6. Unusual start codons were identified in COX1 (GTG) and ATP6 (TTG). Six genes (ND2, COX2, COX3, ND3, ND4, and CytB) were terminated by an incomplete stop codon (TA−/T-). A phylogenetic study showed that Glossogobius formed a clade distinct from other species in the subfamily Gobiinae. G. aureus was most closely related to G. giuris with 87.04% sequence identity among the four species in the genus Glossogobius.
Background: India has a rich aquatic diversity spreading across different ecosystems. Coral reefs are unique with the highest fish diversity and provide nursery and breeding grounds to marine fishes. ...India has fringing reefs around the islands in the Gulf of Mannar, the Gulf of Kutch, and the Andaman and Nicobar Islands. However, these reefs are declining due to anthropogenic activities, and the fish diversity from these reefs has not yet been characterized using molecular markers. Results: In this study, DNA barcodes (cytochrome c oxidase subunit I (COI)) were developed for 50 species of fish representing Ambassidae, Bothidae, Coryphaenidae, Gobiidae, Lutjanidae, Labridae, Pomacentridae, Serranidae, and Tetraodontidae collected from the Gulf of Mannar, the Gulf of Kutch, and the Andaman Islands. The average genetic divergence values increased from lower (within species: 0.3%) to higher (between families: 21%) taxonomic relationships. Cryptic diversity was observed in species of Gobiidae, Serranidae, and Pomacentridae. Distance- and tree-based delimitation methods discriminated 97% of fish species with a sufficient barcode gap. Significance: The present results are useful for effective management of reef fishes. The barcodes will enrich the existing database and are useful for estimating phylogenetic diversity values of reef fishes for conservation purpose.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
A number of fish groups, such as Gobiidae, are highly diversified and taxonomically complex. Extensive efforts are necessary to elucidate their cryptic diversity, since questions often arise about ...the phylogenetic aspects of new species. Clarifications about the diversity and phylogeny of the Bathygobius species from the southwestern Atlantic are particularly needed. Evidence has been accumulating on the Brazilian coast regarding the possible presence of new species while doubts remain about the taxonomic status of others. The taxonomic identification of some species of Bathygobius has been problematic, given their generally conservative external morphology, and several species are recognized as cryptic. This situation hinders understanding the real diversity in this taxon. Taken together, genetic, cytogenetic and morphometric analyses have been effective in identifying new species of this genus. Here we describe the karyotypic features and morphological patterns of three Western South Atlantic species of Bathygobius. Furthermore, its cytochrome c oxidase I (COI) gene sequences were compared with those of species from Central America, North America and the Caribbean. The broad analyses performed demonstrated an unsuspected diversity, leading to the identification of an un-described new species (Bathygobius sp.2) and the geographic redefinition of another, Bathygobius sp.1, undoubtedly a branch of B. geminatus, hitherto inaccurately identified as B. mystacium on the coast of Brazil.
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CEKLJ, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
The widespread tropical gobionelline fish genus Oxyurichthys is monophyletic due to its species sharing two characters considered derived within the Stenogobius Group of the Gobionellinae (Gobioidei: ...Gobiidae), a transversely broadened (spatulate) third neural spine that is usually bifid, and no preopercular cephalic lateralis canal. It is most closely related to Oligolepis, also of the Indo-west Pacific, and Ctenogobius, an Atlantic-eastern Pacific genus. Sixteen valid species of Oxyurichthys are redescribed and illustrated and four new species are described, O. limophilus from the western Indian Ocean, O. rapa from French Polynesia, and O. chinensis and O. zeta from the western Pacific. Nineteen species share two additional synapomorphies, a rounded fleshy tongue and a palatine lacking an elongate posterior strut, and form the sister group to the plesiomorphous Oxyurichthys keiensis, known from South Africa and Madagascar. One species, O. stigmalophius, occurs in the western Atlantic. There are no records of this genus from the continental eastern Pacific or the eastern Atlantic. Previous accounts from the Gulf of Guinea region of West Africa are references to Gobionellus occidentalis. Many Oxyurichthys species are limited to shallow estuarine and coastal waters with bottom substrates of silt or other fine sediments, but several are known from depths exceeding 10 m and are often collected by trawling.
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