The long-term effects of the transplant dose, its administration route and repeated faecal microbiota transplantation (FMT) on the outcomes of FMT for patients with irritable bowel syndrome (IBS) are ...unknown. This study included 171 patients (125 females and 46 males): 90 g of donor feces was administered into the large intestine (LI) in 58, into the small intestine (SI) in 57, and into the SI twice (repeated SI) in 56. The patients provided a fecal sample and completed five questionnaires at the baseline and at 2 years after FMT. Fecal bacteria and the dysbiosis index were analyzed using 16S rRNA gene PCR DNA amplification/probe. The response rates at 2 years after FMT were 47.2%, 80.9%, and 76.6% in the LI, SI, and repeated-SI groups, respectively. The response rate was significantly higher in the SI and repeated SI groups than in the LI group. IBS symptoms at 2 years after FMT were less severe in the SI and repeated-SI groups than in the LI group. Fluorescent signals of several bacteria were significantly correlated with IBS symptoms and fatigue after FMT. No long-term adverse events were observed. In conclusion, administering the transplant to the SI increased the long-term response rate and reduced IBS symptom severity compared with administering it to the LI, and led to the long-term colonization of beneficial bacteria. There was no long-term difference between one and two FMT procedures (www.clinicaltrials.gov: NCT04236843).
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The objective of the current study is to characterize dextran purified from Lactobacillus fermentum (Lb4) Isolates of Lactobacillus spp. were tested for dextran
production using mucoidy, ethanol ...precipitation and spectrophotometric method.
Results of the Spectrophotometric method showed that all isolates gave concentrations of dextran ranging from (0.1 to 0.2 mg/ml). Precipitation, purification and
characterization of dextran from L. fermentum (Lb4) were done. Thin-layer
chromatography (TLC), 1H Nuclear Magnetic Resonance (HNMR) Spectroscopy,
Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FE-SEM), Gas Chromatography-mass spectrometry (GC-MS),
Melting Point (MP), Water Solubility index (WSI), and Water Holding Capacity
(WHC) had been used for dextran characterization. Characterization proved that
pure dextran. Nanosphere structure with a nano-diameter range of about (56.00)
nm with molecular weight (73) kDa. According to the melting point test, the purified dextran from L. fermentum had been completely melted at 228°C. The water
solubility index (WSI) had been determined (93.35%) and the water holding
capacity (WHC) (300%).
Keywords: Lactobacillus spp. ; Dextran ; Lactobacillus fermentum
The application of an adaptation strategy for probiotics, which may improve their stress tolerance, requires the identification of the growth range for each parameter tested. In this study, 4 ...probiotics (Lactobacillus acidophilus, Lacticaseibacillus casei, Lacticaseibacillus rhamnosus, and Lactiplantibacillus plantarum) were grown under different pH, NaCl, and sucrose concentrations at 25°C, 30°C, and 37°C. Turbidimetric growth curves were carried out and lag phase duration, maximum growth rate, and amplitude (i.e., the difference between initial and stationary phase optical density) were estimated. Moreover, cell morphology was observed, and cell length measured. The growth response, as well as the morphological changes, were quite different within the 4 species. The L. acidophilus was the most sensitive strain, whereas L. plantarum was shown to better tolerate a wide range of stressful conditions. Frequently, morphological changes occurred when the growth curve was delayed. Based on the results, ranges of environmental parameters are proposed that can be considered suboptimal for each strain, and therefore could be tested. The quantitative evaluation of the growth kinetics as well as the morphological observation of the cells can constitute useful support to the choice of the parameters to be used in an adaptation strategy, notwithstanding the need to verify the effect on viability both in model systems and in foods.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
In recent years the relationship between the intestinal microbiota, the host and chronic non-communicable diseases has brought interest into the study of its formation and maintenance in the host. ...Lactic acid bacteria (BAL) are Gram-positive bacteria with probiotic activity, which have been associated with many health benefits, such as decreased body fat mass and lower risk of type II diabetes mellitus. One of the main colonization mechanisms and bacteria survival strategies is the production of biofilms and the use of prebiotics as substrates to achieve a balance within intestinal microbiota. However, there is not enough evidence to demonstrate the biofilm formation in the presence of agave fructans (AF). This study aimed to evaluate in vitro the biofilm formation in a consortium of lactic acid bacteria: Lactobacillus delbrueckii ssp. lactis, Lactobacillus delbrueckii ssp. bulgaricus y Streptococcus thermophilus in the presence of AF at different concentrations: 0%, 0,1%, 4%, 8% y 16%. The addition of 0,1% of AF correlates with the best capacity for biofilm formation. The findings imply the possibility of modulating the biofilm formation of lactic acid bacteria with AF. These results can contribute positively to the host, by generating intestinal homeostasis, colonization resistance, stability to food digestion and chemical modifications of drugs and carry out beneficial functions to the health.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
This research focused on the impact of four strains of probiotic Lactobacillus spp. i.e. L. rhamnosus (Lr), L. acidophilus (La), L. plantarum (Lp), or L. casei (Lc) individually on ...post-acidification, the viable cell counts (VCC), total phenolic and flavonoid contents (TPC and TFC, respectively), and antioxidant activity of fermented (F) plant-based milk drink made from individual soy (S) and almond (A) milk (M; 100:0 v/v) and their combination (75:25, 50:50, & 25:75; v/v) during 0, 7, 14, & 21 days of storage. All samples showed VCC of 6 log cfu/ml compared to their respective controls (without bacteria; 3–4 log cfu/ml; p < 0.05). TPC significantly increased in the first week for FSM/AM (75:25)-Lr and in the last two weeks for FAM (100)- & FSM/AM (50:50)-Lr. FAM (100)- & FSM/AM (50:50)- Lr exhibited the highest TFC among other samples during storage. La, Lp, or Lc in FSM & FAM (100) increased the radical scavenging activity and FIC ability to about 95% in the last two weeks of storage. Lr in FSM & FAM (100) and FSM/FAM (75:25 & 25:75) enhanced FRAB activity during storage. In conclusion, fermented soy and almond milk and their combination could serve as an effective vehicle for probiotics with antioxidant activity.
•Lr, La, Lp, & Lc in FSM, FAM, & FSM/AM showed VCC of 6 log cfu/ml.•TPC increased significantly in the first week for FSM/AM (75:25)-Lr.•FAM (100)- & FSM/AM (50:50)- Lr exhibited the highest TFC during the storage.•La, Lp, or Lc in FSM & FAM (100) increased radical scavenging activity/FIC ability.•Lr in FSM & FAM (100) and FSM/FAM (75:25 & 25:75) enhanced FRAB activity.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Several Lactobacillus species are accepted as microorganisms with Qualified Presumption of Safety (QPS) in the EFSA’s list. One of them, Lactobacillus plantarum is a widely distributed species with a ...proven probiotic potential and technological relevance. In addition, every strain must complete several requirements, before implementation. Antibiotic susceptibility is one of EFSA’s important criteria regarding the safety of probiotics. The reason is to avoid any possibility of antibiotic resistance genes transfer to opportunistic pathogens in the gut. In the present study 14 Lactobacillus plantarum strains were assessed for susceptibility to 21 antibiotics from different groups. A high number of resistant strains was determined toward 12 antibiotics (penicillins – penicillin, piperacillin; IIIth generation cephalosporins – cefotaxime, ceftriaxone, ceftazidime; glycopeptides – vancomycin; tetracyclines – tetracycline; aminoglycosides – gentamicin; macrolides – clarithromycin; quinolones – nalidixic acid, ciprofloxacin, levofloxacin). Concerning the other tested antibiotics, strain-specific antibiotic-sensitivity patterns were observed. Antibiotic resistance was also discussed as an advantage in the selection of probiotic strains, however only when it is not transferable. Estimated susceptibility patterns of some of tested candidate probiotic strains are also important, considering the use of the latter as agents accompanying antibiotic therapy
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
An increasing interest has risen on the development of active food packaging to improve freshness and avoid external contamination of seafood commodities. The aims of the present study were to ...fabricate carboxymethyl cellulose-sodium caseinate films containing Lactobacillus acidophilus, L. reuteri, L. casei, L. rhamnosus, and Bifidobacterium bifidum, and evaluate their application on quality properties of raw trout fillets during refrigerated storage for 14 days. The physicochemical, mechanical, structural, and morphology properties of designated films were evaluated using standard procedures. The refrigerated fillets were examined for microbial (Enterobacteriaceae, lactic acid bacteria, total viable count, and psychrotrophic bacteria) and chemical (protein carbonyl, total volatile base nitrogen, peroxide value, thiobarbituric acid reactive substances, and biogenic amine formation) properties. There was a negative correlation between the probiotic's viability and storage time (P < 0.05). The probiotic films provoked retardation of microbial growth of fillets compared to the control at the end of storage period. Chemical changes in all treated fillets were significantly lower than untreated group during the study (P < 0.05). It could be concluded that probiotic films could extend shelf life of trout fillets at least two weeks, at the same time, it could be a method to incorporate beneficial probiotic bacteria to the trout fish.
•Application of probiotic CMC-SC films increased the shelf-life of trout fillets.•Probiotic CMC-SC films may be useful as fresh trout fillets packaging.•Probiotic microorganisms affected physic-mechanical properties of films.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Levan is a naturally occurring fructan, the homopolymer of fructose synthesized as
an exopolysaccharide (EPS) in the extracellular matrix of bacteria from various
genera. Many lactic acid bacteria ...produce levans. In this study, 30 isolates of Lactobacillus spp. have been collected from several hospitals located in Baghdad city.
These isolates were 21 Lactobacillus spp. isolates from the vagina of healthy
women (16 Lactobacillus plantarum,5 Lactobacillus acidophilus ) and 9 isolates
(Lactobacillus plantarum) for infant stool samples. All isolates were tested for
levan production using mucoidy and spectrophotometric methods. The optimum
conditions for levan production were studied, including temperature, incubation
time, pH, inoculum size, sucrose concentration, Nitrogen source, and culture medium. The optimum conditions for production were at 37 ºC for 24 h at pH 7 with 4
% inoculum size and 40 g/ 100ml sucrose concentration with the best nitrogen
source pepton, and the best culture medium for levan production was date medium.
Keywords: Levan homopolymer, Lactobacillus spp., screening, optimum conditions
In this study, 25 isolates of Lactobacillus spp. have been collected from several
hospitals in Baghdad city. These isolates were 17Lactobacillus spp. isolates from
the vagina of healthy women as 12 ...Lactobacillus plantarum,5 Lactobacillus acidophilus and 8 isolates of Lactobacillus plantarum were isolated from feces of
healthy infants; The screening for determining levansucrase production was performed by mucoidy and spectrophotometric methods The levansucrase activity
measured when this isolate was cultivated under the optimal conditions; only 14
isolates could produce levansucrase revealed specific activity ranged from 9.41 to
28.12 U/mg and the maximum specific activity was for Lactobacillus plantarumV11, which was selected as best producer isolates. The levansucrase activity
reached its maximum level when this isolate was cultivated under the optimal
conditions, which consisted of using a levansucrase production medium incubated at 37°C for 24 hours at pH 7 with 4 % inoculum size and 40 g/100ml sucrose
concentration with the best nitrogen source was pepton and the best carbon
source was dated.
Keywords: Lactobacillus spp., levansucrase, production, optimum conditions
The aim of this study was to clarify the influence of Lactobacillus spp. on the degree of endometrial inflammation in the postpartum period and the relationship between Lactobacillus spp. and ...pathogenic bacteria in the endometrium of postpartum dairy cows. Endometrial samples were collected from 41 Holstein-Friesian cows at 4 and 8 weeks postpartum using cytobrushes for polymorphonuclear neutrophil (PMN) count and bacterial culture to isolate Lactobacillus spp., Escherichia coli, and Trueperella pyogenes. The 4-week samples were divided into four groups (E + L +), (E + L −), (E − L +), (E − L −) according to whether endometritis was diagnosed (E +) and Lactobacillus spp. was isolated (L +). The diagnostic criterion for cytological endometritis was > 18% PMN. The average PMN% in the E + L + group was lower than that in the E + L-group (P < 0.05) at 8 weeks postpartum. There were no significant correlations between the number of colonies of Lactobacillus spp. and E. coli or between that of Lactobacillus spp. and T. pyogenes. Lactobacillus spp. could reduce PMN% in dairy cows with endometritis during the puerperal period. In conclusion, the intrauterine presence of Lactobacillus spp. may have a positive effect on uterine involution in postpartum dairy cows.
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