A comparison between two sampling methods (swab and "sponge-bag" methods) for the detection of Listeria monocytogenes and Salmonella spp. has been carried out. The sponge-bag method resulted as ...better for the isolation of these bacteria on the equipments, whereas the swab method resulted more effective for the detection of the microorganisms on the surfaces. Besides, the carriers of Salmonella spp. have been identified as the principal source, whereas Listeria monocytogenes is mainly apported by workers and environment
E' stato effettuato un confronto fra due metodi di campionamento (mediante tampone e mediante "sponge-bag") per la ricerca di Listeria monocytogenes e Salmonella spp. Il metodo sponge-bag si e' dimostrato il migliore nell'isolamento di questi batteri sui macchinari, mentre il metodo con tampone si e' rivelato piu' efficace per il rilievo sulle superfici. Inoltre, i portatori di Salmonella spp. sono stati identificati come principali fonti di contaminazione, mentre Listeria monocytogenes deriva principalmente dagli operatori e dall'ambiente di lavorazione
Bacteriologic examination of 69 meat samples (60- from adult horses and 9- from foals) from slaughter areas in the Republic of Belarus was realized. Microbiological indices of cooled meat of foals ...taking into consideration the infestation level were presented. Research results showed that in the impression smears of intact foals there were revealed single bacilliform and coccoid bacteria (1 x 10E-3 CFU/g). Bacteria of intestinal bacterium (0,1 g) and salmonella were not revealed. In the impression smears of weakly infested foals there were stated single rodlike and coccoid bacteria and total number of microorganisms was 1 x 10E-5 CFU/g. In the impression smears of intensively infested foals there were revealed plenty of gram-positive single rodlike and coccoid bacteria and total number of microorganisms was 1 x 10E-6 CFU/g that did not correspond the maximum permissible level (1 x 10E-4 CFU/g). It was shown that meat of intact and weakly infested foals had general microbial invasion within the standard limits and did not causative agents of alimentary toxicosis. Meat of adult intact and weakly infested horses had general microbial invasion within the standard limits and did not contain opportunistic and pathogenic bacteria. In meat of intensively infested horses the index of microbial invasion exceeded the standard requirement and was 1 x 10E-6 CFU/g and could be recommended only for the industrial processing
From December 1999 to December 2000, 450 samples were collected from 150 slaughtered pigs in Northern Italy. Salmonella enterica subsp. enterica was detected in 5.3% of tonsils, 36% of intestinal ...content and 6% of carcasses. The more common serotypes were S. derby, S. bredeney, S. typhimurium, and S. london. Other serotypes were S. coeln, S. anatum, S. seftenberg, S. agona, S. give, and S. india. The carcasses were contaminated only by three serotypes, i. e. S. derby, S. typhimurium and S. bredeney
Dal dicembre 1999 al dicembre 2000, sono stati prelevati 450 campioni da 150 suini macellati in Italia Settentrionale. Nel 5,3% di campioni di tonsille, nel 36,5% di campioni di contenuto intestinale e nel 6% di campioni prelevati dalle carcasse e' stata riscontrata la presenza di Salmonella enterica subsp. enterica. I tipi sierologici piu' comuni erano S. derby, S. bredeney, S. typhimurium e S. london. Altri sierotipi erano S. coeln, S. anatum, S. seftenberg, S. agona, S. give, e S. india. Le carcasse risultavano contaminate solamente da tre sierotipi, cioe' S. derby, S. typhimurium e S. bredeney
Within the planning of surveillance for control of foods, E. coli strains are a big problem for public health services. Identification of enteritogen E. coli strains in different food components is ...very difficult, because the usual methods can't discriminate pathogenic strains from non pathogenic ones. The aim of this research was the characterization of E. coli isolates by means of virulence gene identification. Out of 85 pork samples examined, E. coli was found in 54%; one sample was enteropathogenic
Nell'ambito della programmazione della sorveglianza per il controllo degli alimenti, i ceppi di E. coli rappresentano un problema notevole per i servizi di igiene pubblica. L'identificazione di E. coli enteritogeni nei diversi componenti degli alimenti e' molto difficile, in quanto i metodi correnti non consentono di distinguere i ceppi patogeni da quelli non patogeni. Lo scopo di questa ricerca consisteva nella caratterizzazione di isolati di E. coli per mezzo dell'identificazione dei geni di virulenza. Degli 85 campioni di carne suina esaminati, E. coli e' risultata presente nel 54%; un campione era enteropatogeno
