Bajam se kao i drugi orašasti plodovi smatra izuzetno zdravom hranom. Stoga su u ekološkim uvjetima Ravnih kotara provedena istraživanja prisustva ostataka pesticida i mikotoksina u jezgrama bajama ...sorti Ferragnes, Ferraduela i sjemenjaku bajama. Istraživanja su provedena u 2022. godini, pri čemu su se koristile najosjetljivije metode analize. Korištenjem GC/MS/MS i LC/MS/MS analize na rezidue 402 pesticida, nije utvrđena prisutnost ni jednog pesticida ni u jednom uzorku. Glede mikotoksina, istraženi su ukupni aflatoksini i najopasniji aflatoksin AfB1 i ni u jednom uzorku nisu detektirani. Uz sve nutritivne, intrinzične i ine prednosti ovo je velika dodana vrijednost, budući su mikotoksini utvrđeni u najproizvodnijim zemljama i brojnim trgovinama diljem svijeta. Dobiveni rezultati nam potvrđuju da se bajami bez opterećenja pesticidima i mikotoksinima mogu kategorizirati kao funkcionalna hrana, odnosno koristiti ih više, jer pojačavaju imunitet i preveniraju brojne bolesti kod ljudi. Istraživanja pridonose boljoj valorizaciji bajama proizvedenih u ekološkim uvjetima Ravnih kotara, cjenovnoj stimulaciji za proizvođače i zdravstvenoj dobrobiti za konzumente.
Similary to other nuts, almond is considered to be extremely healthy. In this context, the research was conducted in the ecological conditions of the Ravni Kotari region on the presence of pesticide residues and mycotoxins in the seeds of the Ferragnes and Ferraduela almond varieties, as well as in the almond seedling. The study was done in 2022 and it used the most sensitive analytical methods. Following the GC/MS/MS and LC/MS/MS analysis for 402 pesticide residues, the results showed that no pesticide was detected in any of the samples. As far as mycotoxins are concerned, the analysis was carried out for total aflatoxins and the most dangerous aflatoxin AfB1, none of which were ultimately detected in any sample. In addition to all the nutritional, intrinsic and other advantages, this represents a great added value, since mycotoxins have been found in the most productive countries and in numerous stores around the world. The obtained results confirm that almonds can be categorized as functional food in the absence of pesticides and mycotoxins, i.e., the increased intake should be encouraged, since they strengthen immunity and prevent numerous diseases in humans. The research helps create better valorisation of almonds produced in the ecological conditions of Ravni Kotari region, at the same time benefiting manufacturers through price stimulation and consumers through the suggested health benefits.
Slavonska kobasica je trajna kobasica koje se u seoskim domaćinstvima u Slavoniji proizvodi tradicionalnom tehnologijom, pri kojoj se tijekom zrenja na ovitku proizvoda mogu razviti plijesni. Cilj je ...ovog rada bio identificirati površinske plijesni i utvrditi njihov utjecaj na kvalitetu i zdravstvenu sigurnost proizvoda, uključujući pojavnost mikotoksina okratoksina A (OTA) i aflatoksina B1 (AFB1). Tijekom rada komunicirano je sa šest proizvodnih lokaliteta na području Slavonije. Pripremljeno je 18 uzoraka slavonske kobasice koje su analizirane na kraju proizvodnog procesa u trajanju od tri mjeseca. Ukupno je identificirano 8 različitih vrsta plijesni, među kojima je dominirao rod Penicillium (88,89 %). Osim šest Penicillium vrsta, izolirana je po jedna vrste iz rodova Aspergillus (8,33 %) i Mucor (2,78 %). Identificirani su i mogući izvori OTA i AFB1, Penicillium verrucosum i Aspergillus flavus, a određena je samo prisutnost OTA i to u koncentraciji od 5,10 μg/kg.
Slavonian sausage is a cured sausage produced on rural holdings using traditional technology, in which moulds are permitted to develop on the product surface during the maturation period. The purpose of this study was to identify the surface moulds and determine their influence on product quality and safety, including the occurrence of the mycotoxins ochratoxin A (OTA) and aflatoxin B1 (AFB1), from six production facilities in the Slavonia region. A total of 18 samples of Slavonian sausage were prepared and analysed at the end of the production process, i.e., after three months. A total of eight species of mould were identified, and the dominant genus was Penicillium (88.89%) with six species, one species from the genus Aspergillus (8.33%) and one species from the genus Mucor (2.78%). Possible causative agents of OTA and AFB1 were identified, Penicillium verrucosum and Aspergillus flavus, though only the presence of OTA was confirmed in a concentration of 5.10 μg/kg.
Raw material mixtures in compound feeds may increase the risk of contamination with different mycotoxins, and their intake can lead to interactive toxic effects. As a result, there is a growing ...awareness of the risks posed to human and animal health by the presence of toxins produced by fungi in food and diet. The aim of this study is to assess the impact of the use of a mycotoxin binder (Micotec) on zootechnical performance in broilers. For this purpose, 3000 day-old chicks of the Arbor acres strain originating from the same hatchery were weighed and distributed equally into six groups one control (C) and five experimental (Exp), as five repetitions receiving the mycotoxin binder Micotec at a dose of 0.1 kg. All subjects (control and experimental groups) were fed a standard staple food suitable for each phase of rearing. The results showed significant differences between the experimental groups and the control one. The best zootechnical performances were recorded in experimental groups receiving the mycotoxin binder, compared to the control. The average weight in the finishing phase for the experimental groups was 3083.6 ± 140.7 g/subject, which was higher than the control group (2800 g/subject) (P<0.05). The consumption index was 1.62 ± 0.08% in the experimental groups, which was 1.84% higher than in chicks of the control group, positively impacting the profitability of breeding. In addition, a lower mortality rate was recorded in the experimental groups than the control (3.01 ± 0.08% vs 7.78%). According to these results, Micotec appears to be a promising additive in improving the zootechnical performance of broilers by adsorbing several types of mycotoxins and reducing their toxic power to ensure safe feed for animals and minimise economic losses caused by these contaminants.
Mješavina različitih sirovina u krmnim smjesama može povećati rizik od kontaminacije s nekoliko mikotoksina, a njihov unos može dovesti do interaktivnih toksičnih učinaka. Kao rezultat, zbog prisutnosti toksina koje proizvode gljivice u hrani i prehrani postoji sve veća svijest o rizicima za zdravlje ljudi i životinja. Cilj je ove studije bio procijeniti učinak uporabe sredstva za vezivanje mikotoksina (Micotec) na zootehničku učinkovitost u tovnih pilića. U tu je svrhu, tri tisuće (3000) pilića soja Arbor acres starih jedan dan iz iste valionice izvagano je i homogeno podijeljeno u 6 skupina (1 kontrolna (C) i 5 eksperimentalnih (Exp) koje su uključivale 5 ponavljanja primanja sredstva za vezivanje mikotoksina Micotec) u dozi od 0,1 kg. Svi pilići i iz kontrole i iz eksperimentalnih skupina hranjeni su standardnom osnovnom hranom prikladnom za svaku fazu uzgoja. Dobiveni rezultati pokazali su značajne razlike između eksperimentalnih skupina i kontrolne skupine. Naime, bolja zootehnička učinkovitost u usporedbi s kontrolnom skupinom zabilježena je u eksperimentalnim skupinama koje su primale sredstvo za vezivanje mikotoksina. Prosječna masa u završnoj fazi za eksperimentalne skupine bila je 3083,6 ± 140,65 g / piliću što je više u usporedbi s kontrolnom skupinom (2800 g / piliću) (P<0,05). Osim toga, indeks potrošnje bio je 1,62 % ± 0,078 u eksperimentalnim skupinama što je bolje u usporedbi s onim zabilježenim u pilića u kontrolnoj skupini (1,84 %), a to pokazuje pozitivan učinak na profitabilnost uzgoja; uz to je i niska stopa smrtnosti zabilježena u eksperimentalnim skupinama (3,01 % ± 0,078 u usporedbi sa 7,78 % za kontrolnu skupinu). Prema ovim rezultatima, Micotec se čini kao obećavajući aditiv u poboljšanju zootehničke učinkovitosti tovnih pilića apsorpcijom nekoliko vrsta mikotoksina i smanjenjem njihove toksičnosti osiguravajući sigurnu hranu za životinje, a koja smanjuje i ekonomske gubitke prouzročene tim mikotoksinima.
Mikroorganizmi su neizbježno prisutni u ječmu, sladu, sladovini, a ponekad i u gotovom pivu. Uzročnici su brojnih problema u pivarskoj industriji. Mikrobna kolonizacija ječma javlja se već u polju ...tijekom vegetacijskog perioda ječma. Mikroflora na ječmu i od njega dobivenog slada utječe na brojne aspekte kvalitete samog proizvodnog procesa u sladarama i pivovarama i na kvalitetu gotovog piva. Osim toga, prisutnost mikrobne kontaminacije na ječmu rezultira stvaranjem antimikrobnih spojeva, čija prisutnost ukazuje na stres tijekom vegetacijskog perioda uzgoja ječma uzrokovanog mikrobnom kontaminacijom. Ako prežive proces kuhanja sladovine ovi spojevi mogu imat negativne ili pozitivne učinke na daljnji proces proizvodnje i na gotovo pivo. Nadalje mikroorganizmi prisutni na ječmu mogu i sami proizvoditi antimikrobne spojeve protiv drugih mikroorganizama koji također migu utjecati i na kvasac i na sam proces proizvodnje slad i piva. U ovom radu dan je osnovni pregled utjecaja mikroflore ječma na proces slada i kvalitete slada, proizvodnju sladovine i njezinu kvalitetu, proces vrenja i utjecaja na aktivnost pivarskog kvasca te na konačni proizvod, pivo.
Microorganisms are inevitably present in barley, malt, wort, and sometimes in finished beer. They are the cause of numerous problems in the brewing industry. Microbial colonization of barley occurs already in the field during the vegetation transition of barley. The microflora on barley and the malt obtained from it affects numerous aspects of the quality of the production process in malthouses and breweries and on the quality of the finished beer. In addition, the presence of microbial contamination on barley results in the formation of antimicrobial compounds, the presence of which indicates stress during the vegetative period of barley cultivation caused by microbial contamination. If they survive the wort brewing process, these compounds can have negative or positive effects on the further production process and on the finished beer. Furthermore, the microorganisms present on barley can themselves produce antimicrobial compounds against other microorganisms that can also affect the yeast and the malt and beer production process itself. This paper provides a basic overview of the influence of barley microflora on the malting process and malt quality, wort production and its quality, the fermentation process and its influence on the activity of brewer's yeast and on the final product, beer.
Tijekom posljednjeg desetljeća znanstven- ici sve više ukazuju na globalno zagrijavanje, dovodeći klimatske promjene u poveznicu s novom strukturom vrsta plijesni producenata mikotoksina po ...geografskim područjima svi- jeta. Izraženije klimatske promjene mogu u budućnosti izmijeniti otpornost domaćina i interakciju domaćin-patogen te imati znatan utjecaj na razvoj toksikogenih plijesni i produk- ciju mikotoksina kao njihovih sekundarnih metabolita. Novonastala klimatološka situ- acija izaziva sve veću pozornost i zahtijeva nove dijagnostičke alate, kao i nove spoznaje o biološkim karakteristikama poljoprivrednih kultura koje se uzgajaju i genetici toksikogenih plijesni. Zbog sve povoljnijih klimatskih uvjeta za rast Aspergillus flavus na području Europe, u narednom razdoblju postoji povećani rizik od onečišćenja žitarica izrazito toksičnim aflatok- sinima. Ujedno, profil mikotoksigenih vrsta Fusarium počinje se sve znatnije mijenjati, a posebno je zabrinjavajući porast onečišćenja s F. graminearum u sjevernom i središnjem dije- lu Europe. S obzirom na očigledne klimatske promjene, potrebna su daljnja istraživanja koja uključuju „prediktivne modele“ s više vari- jabli, a koji bi u bliskoj budućnosti omogućili kvalitetni pristup strategiji prevencije kontam- inacije toksikotvornim plijesnima i mikotok- sinima u hrani i hrani za životinje.
Over the last decade, scientists have increasingly pointed to global warming, linking climate change to a new structure of mycotoxin-producing mould species across the world. In the future, more pronounced climate changes may alter host resistance and host-pathogen interaction, and have a significant impact on the development of toxicogenic moulds and the production of mycotoxins as their secondary metabolites. The newly emerging climatological situation is attracting increasing attention, demanding new diagnostic tools and new insights into the biological characteristics of cultivated crops and the genetics of toxicogenicmoulds. In Europe, the climate conditions are highly favourable for the growth of Aspergillus flavus, bringing increased future risk of contamination of cereals with highly toxic aflatoxins. Meanwhile, the profile of mycotoxigenic species of Fusarium is beginning to change significantly, and increased reports of contamination with F. graminearum in northern and central Europe are particularly worrisome. Given the obvious climate change, further research is needed that will include predictive models with several variables, to provide an appropriate future approach to the strategy of preventing contamination by toxic moulds and mycotoxins in food and feed.
Toksični učinci mikotoksina Lešić, Tina; Šarkanj, Bojan; Kiš, Maja ...
Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam,
11/2020, Volume:
15, Issue:
1-2
Journal Article
Open access
Mikotoksini predstavljaju prirodne toksine koji učestalo kontaminiraju hranu i hranu za životinje, a primarno ih sintetiziraju plijesni iz rodova Aspergillus, Penicillium, Fusarium, Alternaria i ...Claviceps. Ove tvari predstavljaju vrlo stabilne spojeve, otporne na različite uvjete proizvodnje, prerade i skladištenja hrane. Mikotoksini mogu prouzročiti niz štetnih učinaka po zdravlje ljudi i životinja, tzv. mikotoksikoze, uključujući karcinogenost, mutagenost, genotoksičnost, imunotoksičnost, neurotoksičnost, hepatotoksičnost, nefrotoksičnost, te druge, pri čemu često istovremeno djeluju na više ciljnih mjesta u organizmu, u ovisnosti o toksičnosti samog mikotoksina te dozi i vremenu izloženosti.
Za veliki broj mikotoksina još uvijek nema dovoljno dokaza o njihovim štetnim učincima na ljudsko zdravlje, ali se temeljem utvrđene osjetljivosti različitih životinjskih vrsta pretpostavlja da i u ljudi mogu izazvati slična djelovanja. Ujedno, za većinu mikotoksina koji se učestalo zajednički pojavljuju u hrani i hrani za životinje nisu razjašnjeni njihovi učinci u organizmu koji mogu varirati
od antagonističkih do sinergističkih. Ovaj rad daje pregled mehanizama djelovanja mikotoksina od najvećeg javnozdravstvenog značaja i njihovih toksičnih učinaka u organizmu.
Mycotoxins are naturally occurring toxins that frequently contaminate food and feed and are primarily synthesized by moulds from the genera Aspergillus, Penicillium, Fusarium, Alternaria and Claviceps. These substances are very stable compounds, resistant to different conditions of food production, processing and storage. Mycotoxins can cause a number of adverse effects on human and animal health, so-called mycotoxicoses, including carcinogenicity, mutagenicity, genotoxicity, immunotoxicity, neurotoxicity, hepatotoxicity, nephrotoxicity, and others, often acting simultaneously at multiple target sites in the body, depending on the toxicity of the mycotoxin itself and the dose and time of exposure. A large number of mycotoxins still lack sufficient evidence of their adverse effects on human health, but based on the established susceptibility of different animal species, it is assumed that similar effects can be induced in humans. At the same time, for most mycotoxins that occur frequently in food and feed, their effects in the body have not been elucidated and can vary from antagonistic to synergistic. This paper provides an overview of the mechanisms of action of mycotoxins of major public health importance and their toxic effects in the body.
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Microfiltration is a common step in liquid chromatography – tandem mass spectrometry (LC-MS), a method of choice in determining several mycotoxins in a solution at once. However, microfiltration may ...entail filter-analyte interactions that can affect the accuracy of the procedure, and underestimate exposure. The aim of our study was to assess how five different membrane materials for syringe filters (nylon, polytetrafluoroethylene, polyethersulphone, mixed cellulose ester, and cellulose acetate) affect microfiltration and recovery of EU-regulated mycotoxins, including aflatoxins B1, B2, G1, and G2, deoxynivalenol, fumonisins B1 and B2, zearalenone, T-2 and HT-2 toxins, and ochratoxin A. Polytetrafluoroethylene filters turned out to least affect microfiltration through mycotoxin loss, followed by more commonly used nylon filters, whereas the remaining three filter membrane materials had such a negative effect on recoveries that we found them incompatible with the procedure. Our findings clearly suggest that it is important to select a proper filter type that suits analyte properties and solution composition and to discard the first few filtrate drops to ensure the accuracy of the analytical procedure.
Contaminants of Medicinal Herbs and Herbal Products Kosalec, Ivan (Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia); Cvek, Josipa (Agency for Medicinal Products and Medical Devices, Zagreb, Croatia); Tomić, Siniša (Agency for Medicinal Products and Medical Devices, Zagreb, Croatia)
Arhiv za higijenu rada i toksikologiju,
12/2009, Volume:
60, Issue:
4
Journal Article
Peer reviewed
Open access
Medicinal plants have a long history of use in therapy throughout the world and still make an important part of traditional medicine. Thus, medicinal plants and herbal products must be safe for the ...patient (consumer). This review addresses biological contaminants (microbes and other organisms) and chemical contaminants (mycotoxins, toxic elements such as heavy metals, and pesticide residues) as major common contaminants of medicinal herbs and herbal products. To prevent and screen for contamination and ensure safety and conformity to quality standards, medicinal herbs and herbal products should be included in appropriate regulatory framework.
Ljekovito bilje i biljni proizvodi već tisućljećima nalaze široku primjenu u razli _itim sustavima tradicionalnog lije _enja. Stoga je njihova neškodljivost, ponajprije uvjetovana kakvoćom biljne sirovine, od izuzetne važnosti za javno zdravstvo. Od mogućih _imbenika koji utje _u na kakvoću ljekovitog bilja i biljnih proizvoda ovaj pregledni rad osvrće se na naj _ešće prisutna biološka (mikroorganizmi) i kemijska one _išćenja (mikotoksini, toksi _ni elementi poput teških metala te ostaci pesticida). S ciljem postizanja ujedna _enih standarda kakvoće te osiguranja sigurnosti primjene biljnih proizvoda od vitalne su važnosti zakonski propisi koji moraju u odgovarajućim regulatornim okvirima obuhvatiti ovu skupinu proizvoda s naglaskom na sprje _avanju i ispitivanju njihovih mogućih one _išćenja.
Ochratoxin A (OTA) and citrinin (CTN) are nephrotoxic mycotoxins often found together in grain. The aim of this study was to measure their accumulation in the kidney and liver of adult male Wistar ...rats, see how it would be affected by combined treatment, and to determine if resveratrol (RSV) would decrease their levels in these organs. The rats received 125 or 250 mg/kg bw of OTA by gavage every day for 21 days and/or 20 mg/kg bw of CTN a day for two days. Two groups of rats treated with OTA+CTN were also receiving 20 mg/kg bw of RSV a day for 21 days. In animals receiving OTA alone, its accumulation in both organs was dose-dependent. OTA+CTN treatment resulted in lower OTA but higher CTN accumulation in both organs at both OTA doses. RSV treatment increased OTA levels in the kidney and liver and decreased CTN levels in the kidney. Our findings point to the competition between CTN and OTA for organic anion transporters 1 and 3.
Mikotoksini su sekundarni metaboliti plijesni koji predstavljaju značajan problem u području sigurnosti hrane te predstavljaju rizik za zdravlje te dobrobit ljudi i životinja. Učinkovitost metoda ...uklanjanja mikotoksina iz hrane i hrane za životinje, primarno žitarica kao najviše
kontaminirane grupe hrane, ovisi o brojnim parametrima, od kojih su vrlo značajni svojstva onečišćenog materijala, odnosno njegov sastav, primarno sadržaj vode, te razina onečišćenja. Ovaj rad daje pregled fizikalnih i kemijskih metoda koje se manje ili više učinkovito mogu koristiti u tretmanu različitih vrsta hrane i hrane za životinje u cilju
redukcije odnosno uklanjanja mikotoksina. Uklanjanje mikotoksina fizikalnim metodama uključuje njihovu ekstrakciju pomoću otapala, adsorpciju te toplinsku inaktivaciju ili inaktivaciju ozračivanjem. Najznačajnije metode su sortiranje po boji i gustoći, ljuštenje i mljevenje, flotacija, blanširanje, prženje, a u posljednje vrijeme vrlo značajna je primjena gama zračenja te hladne plazme. Uporabom kemijskih metoda koje podrazumijevaju primjenu kemikalija dolazi do konverzije mikotoksina u druge manje toksične spojeve, npr. kiseline, lužine, oksidanse, bisulfite i plinove, no moguća je konverzija i u toksičnije spojeve, što je još uvijek predmet brojnih istraživanja. Za razliku od fizikalnih metoda, kemijske metode uklanjanja mikotoksina u načelu se smatraju nepraktičnim i nepoželjnim, zbog uvjeta provedbe, stvaranja toksičnih ostataka te negativnog utjecaja na nutritivna, senzorska i funkcionalna svojstva proizvoda.
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