Background and Objectives We previously developed a novel additive solution (M‐sol) with a high ability to preserve the in vitro qualities of platelets (PLTs) in washed PLTs Here, we compared the ...ability of M‐sol with that of commercially available additive solutions (ASs) to preserve the in vitro qualities (pH, mean PLT volume, %disc, P‐selectin, %hypotonic shock response and aggregation) of PLTs at a low plasma concentration.
Materials and Methods The platelet concentrate was divided into two equal aliquots (control group and test group). After centrifugation of both groups and removal of as much supernatant as possible, the pellet of the control group was resuspended in M‐sol and those of the test group were resuspended in other ASs, and subsequently stored in polyolefin bags with agitating at 20–24°C.
Results Compared with those stored in M‐sol, the qualities of PLTs stored in PAS‐B (alternative name; PAS‐II or T‐sol), PAS‐ C (alternative name; PAS‐III or Intersol) or Plasma Lyte were degraded as early as 24 h after washing. The qualities of PLTs stored in PAS‐D (alternative name; Composol PS) or PAS‐E (alternative name; PAS‐IIIM or SSP+) were comparable to that of those stored in M‐sol 24 h after washing; however, the qualities had deteriorated 72 h after washing.
Conclusions At a low plasma concentration (5% or less), the M‐sol showed a higher ability to preserve PLTs than the five ASs studied here. Although PAS‐D and PAS‐E are available as an AS for short‐term storage of washed PLTs, M‐sol is thought to be preferable for longer storage.
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BFBNIB, DOBA, FZAB, GIS, IJS, IZUM, KILJ, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBMB, UILJ, UKNU, UL, UM, UPUK
The diagnosis of placenta accreta spectrum (PAS) in pregnancy is associated with severe maternal complications and is potentially life threatening. Moreover, PAS is difficult to diagnose in the first ...trimester, and the accuracy is low. A prenatal diagnosis is pivotal for planning an optimal management in PAS. A first trimester US(Ultrasound) can detect PAS in good proportion of cases, although the sensitivity is lower than a second or third trimester US. An early first trimester US can further help predict severity of PAS and its surgical outcome. As the management and diagnosis remains a challenge as far as PAS in early pregnancy is concerned, a high clinical suspicion especially in cases of previous uterine scar and bleeding following a surgical evacuation cannot be overemphasised. We report a case of Placenta Percreta in First Trimester, its rarity and diagnostic dilemma.
Polyurethane foam (PUF) disks in passive air samplers (PAS) and passive dry deposition (Pas-DD) collectors were used to assess the presence of persistent organic pollutants (POPs) and current-use ...pesticides (CUPs) in a rural area of central Chile (Peumo, VI Region). The samplers were exposed from September 2015 (spring) to March 2016 (summer), with the PUFs collected at intervals of 30, 60, and 90 days. Both samplers (PUF-PAS and Pas-DD) captured more than one pesticide per sampling period. Chlorpyrifos-ethyl and pyrimethanil presented the highest air concentration with PUF-PAS (3470.2 ng m−3 for chlorpyrifos-ethyl and 52.8 ng m−3 for pyrimethanil). The deposited amount of chlorpyrifos-ethyl, pyrimethanil, penconazole, diazinon and malathion in some Pas-DD, was superior to amount of pesticides captured by PUF-PAS. Differences between the amount deposited and captured by each sampler should be studied in greater detail, because wind speed, atmospheric particulate matter size and sampler design are some fundamental variables in this process. These results provide preliminary information on the presence of current-use pesticides in the atmosphere of Peumo, VI Region, serving as a foundation for future environmental monitoring programs.
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•Passive air samplers (PUF-PAS and Pas-DD) are useful tool for CUPs determination in rural areas.•First work that reveals the presence of multiple CUPs in a rural area of Chile•Chlorpyrifos ethyl and pyrimethanil were the compounds detected in highest concentration.•First study to report CUPs in air using Pas-DD samplers•Air pesticide monitoring is fundamental to protect the health of the population in rural areas.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Luteoviruses (family
) and poleroviruses (family
) are economically important pathogens of cereals such as wheat (
), barley (
) and oat (
). In Australia, the luteoviruses barley yellow dwarf virus ...PAV (BYDV PAV) and barley yellow dwarf virus MAV (BYDV MAV), along with the poleroviruses cereal yellow dwarf virus RPV (CYDV RPV) and maize yellow dwarf virus RMV (MYDV RMV), were distinguished from each other and reported in the 1980s (Sward and Lister 1988; Waterhouse and Helms 1985). The poleroviruses barley virus G (BVG) and cereal yellow dwarf virus RPS (CYDV RPS) were reported in Australia more recently (Nancarrow et al. 2019; Nancarrow et al. 2023), while the luteovirus barley yellow dwarf virus PAS (BYDV PAS) has not previously been reported in Australia. During 2010, an oat plant exhibiting yellow/ red leaf discoloration and stunted growth was collected from a roadside in Horsham, Victoria, Australia. The plant tested positive for BYDV PAV and negative for BYDV MAV, CYDV RPV and MYDV RMV by tissue blot immunoassay (TBIA) as described by Trębicki et al (2017). The virus isolate has since been continuously maintained in a glasshouse in live wheat plants using aphids (
). In 2021, total RNA extracted from a wheat plant infected with this isolate (Nancarrow et al. 2023) tested positive for BYDV PAV by RT-PCR using the primers BYDV-1/BYDV-2 (Rastgou et al. 2005), but negative for BYDV PAV, CYDV RPV and MYDV RMV using other published primers (Deb and Anderson 2008). A high-throughput sequencing (HTS) library was prepared from the total RNA with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) without ribosomal RNA depletion and sequenced on a NovaSeq 6000 (Illumina). Raw reads were trimmed and filtered using fastp v0.20.0 (Chen et al. 2018) while de novo assembly of all of the resulting 5,049,052 reads was done using SPAdes v3.15.3 (Nurk et al. 2017). BLASTn analysis of the resulting 4,067 contigs (128- 12,457 bp in length) revealed only one large virus-like contig (5,649 bp) which was most similar to BYDV PAS isolates on NCBI GenBank, sharing 87% nucleotide (nt) identity with BYDV PAS isolate OH2 (MN128939), 86% nt identity with the BYDV PAS reference sequence (NC_002160) and 82% nt identity with the BYDV PAV reference sequence (NC_004750). Additionally, 4,008 HTS reads were mapped to the assembled genome sequence with Bowtie2 v2.4.5. (Langmead and Salzberg 2012) with 100% genome coverage and an average coverage depth of 101X. Primers were designed to the assembled genome sequence to generate overlapping amplicons across the genome, and the resulting amplicons were Sanger sequenced. This confirmed the genome sequence of BYDV PAS isolate PT from Australia (5649 bp, GC content 47.9%), which was deposited in GenBank (LC782749). Ten additional plant samples collected from western Victoria, Australia, all tested positive for BYDV PAS by RT-PCR using the primers PASF and PASR (Laney et al. 2018). The additional samples consisted of one oat sample collected in 2005, one barley sample collected in 2007, three wheat samples collected in 2016 and one barley, one brome grass (
sp.) and three wheat samples collected in 2020. BYDV PAS is also efficiently transmitted by
but is often more prevalent and severe than BYDV PAV; it can also overcome some sources of virus resistance that are effective against BYDV PAV (Chay et al. 1996, Robertson and French 2007). To our knowledge, this is the first report of BYDV PAS in Australia. Further work is needed to determine the extent of its distribution, incidence, impacts and epidemiology in Australia, along with its relationship to other BYDV PAS isolates.
Green chemistry principles (GCP) are comprehensively deployed in industrial management, governmental policy, educational practice, and technology development around the world. Circular economy always ...aims to balance the economic growth, resource sustainability, and environmental protection. This article offers a highlight on issues of significance within GCP and circular economy, and proposes the integrated strategies for GCP implementation from the aspects of governance, industry and education. At first, we developed a new categorizing system for GCP dividing to (i) pollution and accident prevention, (ii) safety and resource sustainability, and (iii) energy and resource sustainability. To assess the GCP practice towards the circular economy, the implementation of international movement of GCP in worldwide policy, especially those of Canada, China, Germany, Japan, South Korea, Sweden, Taiwan, United States and United Kingdom were reviewed. The policy implementation of GCP practices among governance, industries and education was analyzed. To integrate GCP into the circular economy concept, we also proposed five strategies of priority governance direction as follows: (i) establishment of cross-departmental collaboration, (ii) development of cleaner production and green product, (iii) provision of integrated chemical management system, (iv) implementation of green chemistry education program, and (v) construction of a business model. Finally, we discussed the prospects of disciplinary elements including the establishment of redesign-reduction-recovery-recycle-reuse (5R) practices for wastes reclamation, deployment of water-energy-food nexus with GCP to improve the food security and resource sustainability, and implementation of GCP in the green smart industrial park.
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•Prevention, assurance and sustainability are key indexes for green chemistry.•International movement of green chemistry on policy implementation was reviewed.•Integrated management and innovative technology of green chemistry were presented.•Connection between green chemistry principles and circular economy was eatablished.•Strategies on green chemistry principles towards circular economy were provided.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
W listopadzie 2016 r. Instytut Niskich Temperatur i Badań Strukturalnych PAN obchodził uroczyście 50-lecie swojego istnienia. Przedstawiony został zarys historii kilkunastu lat wcześniejszego ...istnienia dziesięciu placówek naukowych, z których ostatecznie w 1966 r. powstał Instytut. Pokazano zmagania profesorów Romana Ingardena i Włodzimierza Trzebiatowskiego o utworzenie we Wrocławiu silnego ośrodka fizyki i fizykochemii ciała stałego.Institute of Low Temperature and Structure Research of Polish Academy of Sciences celebrated its 50th anniversary in November 2016. The paper presents the history of the Institute going backward to the history of other ten scientific institutions from which the Institute was finally founded in 1966. It shows the efforts of Prof. Roman Ingarden and Prof. Włodzimierz Trzebiatowski to establish a powerful center of physics and physico-chemistry of solid state in Wrocław.
Institute of Low Temperature and Structure Research of Polish Academy of Sciences celebrated its 50th anniversary in November 2016. The paper presents the history of the Institute going backward to ...the history of other ten scientific institutions from which the Institute was finally founded in 1966. It shows the efforts of Prof. Roman Ingarden and Prof. Włodzimierz Trzebiatowski to establish a powerful center of physics and physico-chemistry of solid state in Wrocław.
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•Compositional analysis of gallstone samples has been analysed using LIBS and PAS.•Molecular signatures of CaO Orange band, CN Violet band, and C2 Swan band are confirmed in LIBS ...spectra alongwith elemental signature.•Pigment gallstone to be more rigid than mixed gallstone is confirmed by LIBS.•Classification of gallstone was done by PCA on the LIBS data.•Presence of cholesterol, calcium carbonate, calcium phosphate, bile acid, bilirubin, and fatty acid are confirmed by PAS.
Compositional analysis of gallstone samples has been carried out, using Laser-Induced Breakdown Spectroscopy (LIBS) and Photoacoustic Spectroscopy (PAS). Classification of gallstone has been made on the basis of intensities of the inorganic and organic constituents present in the LIBS spectra. A regression plot is drawn between LIBS spectral intensities of organic & inorganic elements and the stoichiometric ratio of Cholesterol, Bilirubin and Calcium Carbonate. Atomic lines of various elements, as well as molecular signatures of CaO Orange band, CN Violet band, and C2 Swan band, are observed in LIBS spectra. The relative hardness of gallstones is estimated from the intensity ratio of ionic to neutral atomic lines of the species observed in LIBS spectra. PAS is used for detecting molecular constituents in the gallstones. Principal Component Analysis (PCA) is performed for the discrimination of gallstones. It is found that PAS data, in combination with LIBS provide a suitable method for the compositional analysis of gallstones.
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