Adult-onset Still's disease (AOSD) patients may show an evanescent salmon-pink erythema appearing during febrile attacks and reducing without fever. Some patients may experience this eruption for ...many weeks. During AOSD, exceptionally high serum levels of ferritin may be observed; it is an iron storage protein composed of 24 subunits, heavy (H) subunits and light (L) subunits. The ferritin enriched in L subunits (L-ferritin) and the ferritin enriched in H subunits (H-ferritin) may be observed in different tissues. In this work, we aimed to investigate the skin expression of both H-and L-ferritin and the number of macrophages expressing these molecules from AOSD patients with persistent cutaneous lesions. We observed an increased expression of H-ferritin in the skin, associated with an infiltrate in the biopsies obtained from persistent cutaneous lesions of AOSD patients. Furthermore, a positive correlation between H-ferritin skin levels as well as the number of CD68 super(+)/H-ferritin super(+) cells and the multi-visceral involvement of the disease was observed. Our data showed an increased expression of H-ferritin in the skin of AOSD patients, associated with a strong infiltrate of CD68 super(+)/H-ferritin super(+) cells. Furthermore, a correlation between the levels of H-ferritin as well as of the number of CD68 super(+)/H-ferritin super(+) cells and the multi-visceral involvement of the disease was observed.
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BFBNIB, DOBA, FZAB, GIS, IJS, IZUM, KILJ, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBMB, SIK, UILJ, UKNU, UL, UM, UPUK
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
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A role of salmon SOSC1 in antiviral immunity? Sobhkhez, Mehrdad; Joensen, Lisbeth; Tollersrud, Linn G. ...
Fish & shellfish immunology,
June 2016, 2016-06-00, 20160601, Volume:
53
Journal Article
Peer reviewed
Open access
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
ObjectiveTo investigate whether the administration of antibiotics affect faecal excretion time in paediatric salmonellosis with different severity.MethodsChildren with nontyphoid salmonellosis ...admitted to Kaohsiung Veterans General Hospital, Taiwan who consented to receive consecutive stool cultures every 4-7 days till two consecutive negative results between 2005 and 2013 were enrolled. Faecal excretion time was defined as the timeframe of the first positive stool culture and the first of two consecutive negative results. The Severity Score was used to stratify the patients as the severe, moderate and mild group according to their fever days before admission, band cells in peripheral blood and C-reactive protein. Patients were classified into no antibiotics (mild disease without antibiotic), concordant (severe patients receiving antibiotics active in vitro) and inappropriate therapy group (mild patients receiving antibiotics or severe patients receiving antibiotics resistant in vitro).Results53 patients were enrolled. Compared with no antibiotic group (n = 28), the concordant group (n = 11) had comparable faecal excretion time (12.36 vs 9.79 days), fever days after admission and hospital stay (2.45 vs 2.25; 6.45 vs 6.39 days). The inappropriate group (n = 14) had significantly longer faecal excretion time (24.50 vs 9.79 days), hospital stay (8.50 vs 6.39 days) and higher platelet count (333.43 vs 269.82 1,000/mm3) than no antibiotics group. With further multiple regression analysis, only inappropriate antibiotic use affected significantly (p = 0.008) on faecal excretion time.ConclusionsAntibiotics used in severe children with salmonellosis didn't prolong faecal excretion time, but inadequate use of antibiotics prolonged faecal excretion time in Salmonellosis.
Ethnopharmacological relevance The medicinal mushroom Antrodia salmonea has been used as a traditional Chinese medicine and has demonstrated antioxidant and anti-inflammatory effects. Materials and ...methods: In the present study, we examined the anti-tumor activity of the fermented culture broth of Antrodia salmonea (AS) in vitro and in vivo and revealed its underlying molecular mechanism of action. Results: Treatment of human promyelocytic leukemia (HL-60) cells with AS (50-150 mu g/mL) significantly reduced cell viability and caused G sub(1) arrest via the inhibition of cell-cycle regulatory proteins, including cyclin D1, CDK4, cyclin E, cyclin A, and phosphorylated retinoblastoma protein (p-Rb). Furthermore, AS treatment induced apoptosis, which was associated with DNA fragmentation, followed by a sequence of events, including intracellular ROS generation; mitochondrial dysfunction; Fas ligand activation; cytochrome c release; caspase-3, -8, -9, and PARP activation; and Bcl-2/Bax dysregulation. The results of the in vitro study suggested that AS-induced apoptosis in HL-60 cells was mediated by both the mitochondrial and death receptor pathways. Furthermore, we found that AS treatment was effective in delaying tumor incidence in HL-60 xenografted nude mice and reducing tumor burden. Conclusions: To the best of our knowledge, this is the first report confirming the anti-tumor activity of this potentially beneficial mushroom against human promyelocytic leukemia.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Nous discutons de l'importance de faire face aux menaces diffuses qui pesent sur les especes a long terme et la viabilite de l'habitat dans la conservation du poisson et la planification du ...retablissement. Dans le Nord-Ouest Pacifique, aux Etats-Unis, les plans de gestion des salmonides ont generalement porte sur l'habitat degrade d'eau douce, les barrages, le passage des poissons, les taux de capture et les lachers. Cependant, ces plans s'attaquent mal aux menaces liees a la population humaine et la croissance economique, la concurrence intra et interspecifique, et les changements des conditions climatiques, de l'ocean, et des estuaires. D'apres les analyses effectuees sur huit plans de conservation et/ou de retablissement, nous avons constate que meme si les menaces resultant de ces changements sont difficiles a modeliser et/ou a prevoir, elles sont particulierement importantes pour un grand nombre d'especes diadromes. La gestion adaptative est egalement un element essentiel, mais souvent mal elabore de ces plans. La gestion adaptative devrait etre concue pour repondre a l'evolution des connaissances sur les poissons et leurs ecosystemes associes; si elle est faite correctement, elle devrait contribuer a ameliorer les efforts de conservation en diminuant l'incertitude concernant les menaces connues et diffuses. Nous concluons par un appel general aux gestionnaires et planificateurs environnementaux pour relancer le processus de gestion adaptative dans les plans de gestion futurs, y compris a identifier plus explicitement les incertitudes critiques, la mise en oeuvre des programmes de surveillance pour reduire ces incertitudes, et en indiquant explicitement les mesures de gestion qui seront mises en oeuvre lorsque les seuils de declenchement preidentifies seront atteints.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Smartphone-based optical detection is a potentially easy-to-use, handheld, true point-of-care diagnostic tool for the early and rapid detection of pathogens. Paper microfluidics is a low-cost, ...field-deployable, and easy-to-use alternative to conventional microfluidic devices. Most paper-based microfluidic assays typically utilize dyes or enzyme-substrate binding, while bacterial detection on paper microfluidics is rare. We demonstrate a novel application of smartphone-based detection of Salmonellaon paper microfluidics. Each paper microfluidic channel was pre-loaded with anti-SalmonellaTyphimurium and anti-Escherichia coliconjugated submicroparticles. Dipping the paper microfluidic device into the Salmonellasolutions led to the antibody-conjugated particles that were still confined within the paper fibers to immunoagglutinate. The extent of immunoagglutination was quantified by evaluating Mie scattering from the digital images taken at an optimized angle and distance with a smartphone. A smartphone application was designed and programmed to allow the user to position the smartphone at an optimized angle and distance from the paper microfluidic device, and a simple image processing algorithm was implemented to calculate and display the bacterial concentration on the smartphone. The detection limit was single-cell-level and the total assay time was less than one minute.
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