A time-based (TB) receiver (RX) with a 2-tap TB decision feedback equalizer (DFE) is proposed for mobile DRAM interface. The TB RX consists of a voltage-to-time converter (VTC), a TB DFE, and a time ...comparator. The VTC converts the RX input voltage to a time difference between two VTC outputs by using the difference in clock-to-Q delays between two latches with different input offset voltages. The TB DFE inserts an additional delay to one of the two VTC outputs and bypasses the other VTC output to increase the time opening. The time comparator makes a decision with the first arriving edge of the two outputs of the TB DFE. While the feedback loop delay must be less than 1 UI for proper operation in the conventional voltage-based DFE, the TB DFE allows the feedback loop delay up to 1.43 UI in this paper. A transmitter (TX) transmits a single-ended signal of 200-mV swing by using an n-over-n voltage-mode driver. The transceiver in a 65-nm CMOS process achieves a 12.5 Gb/s with a 0.8-V supply through a 15-inch FR-4 channel of 14-dB loss. The TX and RX chip consume 4.3 and 3.4 mA, respectively. The energy efficiency is 0.49 pJ/b.
El artículo tiene como objeto el estudio de la situación actual del transporte urbano de viajeros en automóviles de turismo. Se trata de un tema en el que se conjuga la regulación administrativa ...junto con la irrupción de las plataformas digitales y sus aplicaciones en un marco de llamada economía colaborativa. Se aboga por una reforma integral de las actividades de taxi y VTC que tome en consideración las necesidades del transporte de viajeros en automóviles de turismo en las ciudades y permita la compatibilidad y convivencia de ambas modalidades de transporte. Los recientes cambios normativos no parecen que sean los más adecuados y permiten augurar futuros conflictos judiciales.
Polymeric nanoparticles were widely used as delivery vehicles for targeted delivery of anticancer drugs, because of their targeting property and versatility. Mitochondria are one of the important ...organelles that regulate the apoptosis of cancer cells and can be considered as a pivotal target for cancer treatment. A pH-responsive charge-reversal and mitochondrial targeting nanoparticles, Vitamin B6-oligomeric hyaluronic acid-dithiodipropionic acid-berberine (B6-oHA-SS-Ber), were prepared in this study. Ber is a lipophilic cation that was conjugated with oHA through disulfide bonds to produce mitochondria-targeted conjugates (oHA-SS-Ber). B6 was conjugated to oHA to obtain B6-oHA-SS-Ber and the two types of Cur-loaded nanoparticles (Cur-NPs) were formulated by the dialysis method. Due to pKa of B6, the charge they carried in the tumor tissue acidic microenvironment can be transferred from negative charge to positive charge, further targeting mitochondria. In our study, we successfully synthesized B6-HA-SS-Ber and characterized the structure by
H-NMR. According to the results of transmission electron microscopy (TEM), we found that the B6-oHA-SS-Ber/Cur micelles could self-assembled in water to form spherical nanoparticles, with a hydrodynamic diameter of 172.9±13 nm. Moreover, in vitro cytotoxicity, cellular uptake, lysosome escape and mitochondrial distribution researches revealed the better effect of B6-oHA-SS-Ber/Cur micelles in comparison to oHA-SS-Ber/Cur. In vivo anticancer activities indicated that the B6-oHA-SS-Ber/Cur micelles exhibited effective inhibition of tumor growth.
Hydrothermal carbonization (HTC) reactor systems used to convert wet organic wastes into value-added hydrochar are generally classified in the literature as liquid water-based (HTC) or vapor-based ...(VTC). However, the distinction between the two is often ambiguous. In this paper, we present a methodological approach to analyze process conditions for hydrothermal systems. First, we theoretically developed models for predicting reactor pressure, volume fraction of liquid water and water distribution between phases as a function of temperature. The reactor pressure model predicted the measured pressure reasonably well. We also demonstrated the importance of predicting the condition at which the reactor system enters the subcooled compression liquid region to avoid the danger of explosion. To help understand water–feedstock interactions, we defined a new solid content parameter %S(T) based on the liquid water in physical contact with feedstock, which changes with temperature due to changes in the water distribution. Using these models, we then compared the process conditions of seven different HTC/VTC cases reported in the literature. This study illustrates that a large range of conditions need to be considered before applying the label VTC or HTC. These tools can help in designing experiments to compare systems and understand results in future HTC research.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
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•Vitamin B6-E analogue conjugate could form small molecule nanodrug.•Vitamin B6 in shell facilitated long circulation and tumoral cellular uptake.•Charge reversal of vitamin B6 ...improved endosomal escape and drug release.•Synergetic therapy of doxorubicin and α-TOS enhanced cytotoxicity.
To promote the targeted cancer therapy, the pH-sensitive small molecule nanodrug self-assembled from amphiphilic vitamin B6-E analogue conjugate was successfully constructed. Herein, water-soluble vitamin B6 with pKa (5.6) was chemically conjugated to lipid-soluble vitamin E succinate (α-TOS), which showed selective cancer cell killing ability and this amphiphilic small molecule vitamin conjugate could self-assemble to be free nanoparticles (NPs) and doxorubicin-loaded NPs (α-TOS-B6-NPs-DOX). The small molecule nanodrugs could perform the following characteristic: (i) stability in the sodium dodecyl sulfonate (SDS) solution and long-term storage stability in PBS via surface negative charge; (ii) tumor accumulation by enhanced penetration and retention (EPR) effect; (iii) improved cellular internalization by means of vitamin B6 transporting membrane carrier (VTC); and (iv) facilitating endosomal escape and rapid drug release for synergistic toxicity to tumor cells via charge reversal and ester hydrolysis at intracellular pH and/or esterase. Moreover, α-TOS-B6-NPs-DOX exhibited long blood circulation stability and significant tumor accumulation and inhibition with the decreased side effects in vivo. Thus, the pH-sensitive small molecule nanodrug self-assembled from amphiphilic vitamin B6-E analogue conjugate could be the potential drug carriers in targeted synergistic cancer therapy.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Coronaviruses (CoVs) assemble by budding into the lumen of the intermediate compartment (IC) at the endoplasmic reticulum (ER)-Golgi interface. However, why CoVs have chosen the IC as their ...intracellular site of assembly and how progeny viruses are delivered from this compartment to the extracellular space has remained unclear. Here we address these enigmatic late events of the CoV life cycle in light of recently described properties of the IC. Of particular interest are the emerging spatial and functional connections between IC elements and recycling endosomes (REs), defined by the GTPases Rab1 and Rab11, respectively. The establishment of IC-RE links at the cell periphery, around the centrosome and evidently also at the noncompact zones of the Golgi ribbon indicates that-besides traditional ER-Golgi communication-the IC also promotes a secretory process that bypasses the Golgi stacks, but involves its direct connection with the endocytic recycling system. The initial confinement of CoVs to the lumen of IC-derived large transport carriers and their preferential absence from Golgi stacks is consistent with the idea that they exit cells following such an unconventional route. In fact, CoVs may share this pathway with other intracellularly budding viruses, lipoproteins, procollagen, and/or protein aggregates experimentally introduced into the IC lumen.
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A 10-Gb/s on-chip 1-D eye-opening monitor (EOM) for receiver front-end equalizer boost gain adaptations is presented. The proposed EOM circuits report in real-time horizontal eye-openings using ...equalizer output by calculating the probability density of the waveform in the central row of pixels of the eye diagram. In addition, a novel multi-phase generator circuit with a delay gain calibration is also demonstrated. It is suitable for EOM circuits to generate a multi-phase sampling clock. The proposed 1-D-EOM circuit is included in a 10-Gb/s receiver design to verify its adaptation functions, and the circuit is implemented using the 65-nm CMOS technology. The sampling phase resolution is 1.5625 ps (where the time for one bit is 100 ps, with a total of 64 phases), and the response time is 64 μs. The total power consumption of the EOM circuit is 1.5 mW with a 1-V supply voltage, and the circuit occupies a layout area of 60 μm × 450 μm. The results show that the reported horizontal eye-opening value is proportional to the value from a real eye diagram monitor from the test buffer.
Endocytosis and autophagy are the main pathways to deliver cargoes in vesicles and autophagosomes, respectively, to vacuoles/lysosomes in eukaryotes. Multiple positive regulators but few negative ...ones are reported to regulate the entry of vesicles and autophagosomes into vacuoles/lysosomes. In yeast, the Rab5 GTPase Vps21 and the ESCRT (endosomal sorting complex required for transport) are positive regulators in endocytosis and autophagy. During autophagy, Vps21 regulates the ESCRT to phagophores (unclosed autophagosomes) to close them. Phagophores accumulate on vacuolar membranes in both vps21∆ and ESCRT mutant cells under a short duration of nitrogen starvation. The vacuolar transport chaperon (VTC) complex proteins are recently found to be negative regulators in endocytosis and autophagy. Phagophores in vps21∆ cells are promoted to enter vacuoles when the VTC complex proteins are absent. Phagophores are easily observed inside vacuoles when any of these VTC complex proteins (Vtc1, 2, 4, 5) are removed. However, it is unknown whether the removal of VTC complex proteins will also promote the entry of phagophores into vacuoles in ESCRT mutant cells under the same conditions. Snf7 is a core subunit of ESCRT subcomplex III (ESCRT-III), and phagophores accumulate in snf7∆ cells under a short duration of nitrogen starvation. We used green fluorescence protein (GFP) labeled Atg8 to display phagophores and FM4-64-stained or Vph1-GFP-labeled membrane structures to show vacuoles, then examined fluorescence localization and GFP-Atg8 degradation in snf7∆ and snf7∆vtc4∆ cells. Results showed that Vtc4 depletion promoted the entry of phagophores in snf7∆ cells into vacuoles as it did for vps21∆ cells, although the promotion level was more obvious in vps21∆ cells. This observation indicates that the VTC complex proteins may have a widespread role in negatively regulating cargos to enter vacuoles in yeast.
Inorganic polyphosphates (polyP) are the linear polymers of orthophosphoric acid varying in the number of phosphate residues linked by the energy-rich phosphoanhydride bonds. PolyP is an essential ...component in living cells. Knowledge of polyP metabolizing enzymes in eukaryotes is necessary for understanding molecular mechanisms of polyP metabolism in humans and development of new approaches for treating bone and cardiovascular diseases associated with impaired mineral phosphorus metabolism. Yeast cells represent a rational experimental model for this research due to availability of the methods for studying phosphorus metabolism and construction of knockout mutants and strains overexpressing target proteins. Multicomponent system of polyP metabolism in
Saccharomyces cerevisiae
cells is presented in this review discussing properties, functioning, and practical significance of the enzymes involved in the synthesis and degradation of this important metabolite.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Degradation and self-recovery of polycrystalline Silicon (poly-Si) Thin film transistor (TFT) by using complementary metal oxide semiconductor (CMOS) inverter were investigated. Under DC stress, ...degradation mechanisms were clarified by comparing the Voltage transfer characteristics (VTC) of fresh and stressed inverters. It is determined that Negative bias temperature instability (NBTI) of p-TFT dominates the degradation of the inverter under zero bias DC stress. After removing the stress, the VTC continues to be degraded, because the interface trap-states and the grain boundary trapstates increase due to hydrogen species diffusion. It is found out that the VTC is shifted to its right side severely with negative bias stress of VIN. The NBTI of p-TFT is enhanced and the NBTI of n-TFT also plays a role on the degradation. When removing the negative bias stress, the self-recovery of NBTI of nTFT and the continuing degradation of NBTI of p-TFT become competing mechanisms, together controlling the VTC after-stress behavior. Consequently, the continuing degradation of NBTI of p-TFT is restrained by selfrecovery of NBTI of n-TFT.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
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