The widespread use of antibiotics in the poultry industry as growth promoters has led to the emergence of bacterial resistance, which poses a significant health risk to humans and animals. Substances ...of natural origin, such as probiotic bacteria and humic substances, can be a promising solution. The aim of this experiment was to study the effect of the administration of a probiotic strain of Limosilactobacillus fermentum 2i3 and/or a new formula of humic substances specifically designed for detoxification on the production parameters, including gene expression of myogenic growth factors and selected parameters of the immune response. We found that production parameters such as feed conversion ratio and weekly weight gain, as well as gene expression of mucin-2 and immunoglobulin A, were positively influenced mainly by the administration of L. fermentum 2i3. Similarly, the percentage of active phagocytes and their absorption capacity as well as the proportions of CD8+ and CD4+CD8+ T-lymphocyte subpopulations were significantly increased. The addition of humic substances, either alone or in combination with probiotics, significantly reduced the aforementioned parameters compared to the control. On the other hand, the relative gene expression for all myogenic growth factors was the highest in the humic group alone. Based on the results obtained, we can confirm the immunostimulating effect of L. fermentum 2i3 administered in drinking water, which also had an impact on important production parameters of broiler meat. On the other hand, in the combined group there was no expected potentiation of the positive effects on the observed parameters.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ
Sodium dehydroacetate (DHA-Na) is a fungicidal preservative widely used in food and animal feed. DHA-Na can induce coagulation disorders in rats and poultry by inhibiting carboxylation of vitamin ...K-dependent proteins; it can also impair bone development in zebrafish. However, the effects of DHA-Na on broiler chicken bones remain unknown. Here, we assessed whether DHA-Na impairs bone development in broiler chickens. We administered Suji yellow chickens with 200 to 800 mg/kg DHA-Na, 2 mg/kg vitamin K, or both for 2 mo. Bone metabolite–related serum indicators, tissue micromorphology, and relevant protein expression were monitored during the treatment period. We also assessed primary chicken osteoblast activity, differentiation, and bone metabolite–related proteins after treatment with DHA-Na, vitamin K, or both. The results demonstrated that DHA-Na reduced bone index values and serum and bone osteoblast differentiation marker levels but blocked bone vitamin K cycle. DHA-Na also increased serum osteoclast differentiation marker levels, as well as the bone ratio of receptor activator of nuclear factor kappa-Β ligand to osteoprotegerin ratio. Moreover, DHA-Na reduced bone trabecular number, thickness, and area and increased trabecular separation considerably. In general, compared with the control group, the DHA-Na group demonstrated impairments in osteoblast activity and differentiation, as well as in the vitamin K cycle. By contrast, vitamin K supplementation led to considerable attenuation of the DHA-Na-induced decrease in osteogenic marker levels, along with a considerable increase in serum bone absorption marker levels and restoration of DHA-Na-induced bone microstructure damage. Vitamin K also attenuated DHA-Na-induced impairment in osteoclasts. In conclusion, the results indicated that in broiler chickens, DHA-Na supplementation can damage bones by inhibiting osteoblast function and increasing osteoclast activity; this damage can be prevented through vitamin K supplementation.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ
Previously, we reported that glucagon-like peptide-1 (GLP-1) and its analog liraglutide could inhibit fat de novo synthesis in the liver and reduce abdominal fat accumulation in broiler chickens. ...Nevertheless, the impact of GLP-1 on adipocyte fat deposition remains enigmatic. This study aimed to investigate the effects of GLP-1, via its analog liraglutide, on chicken chicken adipocytes in vitro. Chemical assays, quantitative real-time polymerase chain reaction (qRT-PCR), and western blot were employed to assess the proliferation, differentiation, and fat deposition of chicken adipocytes. Our findings indicated that liraglutide significantly suppressed cell proliferation and promoted preadipocyte differentiation in comparison to the control group. This was evidenced by elevated triglyceride (TG) content and upregulated mRNA expression of lipogenesis-related enzymes, such as acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS), as well as regulators including peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element binding protein-1 (SREBP1) and CCAAT/enhancer binding protein α (CEBPα). In mature adipocytes, liraglutide attenuated fat deposition by inhibiting fat de novo synthesis, evidenced by decreased mRNA expression of ACC, FAS, PPARγ, C/EBPα, and SREBP1, and concurrent upregulation of phosphorylated AMP-activated protein kinase (p-AMPK) and phosphorylated ACC (p-ACC). This resulted in reduced accumulation of lipid droplets and TG content in mature adipocytes. Collectively, our findings indicate that liraglutide suppresses the proliferation of preadipocytes, enhances their differentiation, and concurrently inhibits de novo lipogenesis in mature adipocytes. This observation offers profound insights into the mechanisms that underlie liraglutide's anti-adipogenic effects, which could have significant implications for the treatment of obesity in broiler chickens.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ
This trial was conducted to evaluate the effects of replacing soybean meal with microalgae meal (MM; Arthrospira spp.) during grower and finisher phases on productive performance, footpad dermatitis ...(FPD) occurrence, breast meat quality, amino acid digestibility and plasma metabolomics profile of broiler chickens. One thousand day-old Ross 308 male chicks were divided into 5 experimental groups (8 replicates, 25 birds/each): CON, fed a commercial soybean-based diet throughout the trial (0–41 d); F3 and F6, fed the CON diet up to 28 d of age and then a finisher diet (29–41 d) with either 30 or 60 g MM/kg, respectively; and GF3 and GF6, receiving CON diet until 14 d and then diets containing 30 or 60 g MM/kg from 15 to 41 d, respectively. All diets were iso-energetic and with a similar amino acid profile. Growth performances were recorded on a pen basis at the end of each feeding phase and apparent ileal amino acid digestibility was determined at 41 d. Footpad dermatitis occurrence was assessed on all processed birds, while breast and plasma samples were collected for meat quality and metabolomics analysis (proton nuclear magnetic resonance - 1H-NMR). At 41 d, CON group showed higher body weight than F6 and GF6 ones (2,541 vs. 2,412 vs. 2,384 g, respectively; P < 0.05). Overall, GF6 group exhibited the highest feed conversion ratio, while F3 did not present significant differences compared to CON (1.785 vs. 1.810 vs. 1.934 g feed/g gain, respectively for CON, F3 and GF6; P < 0.01). The occurrence and the risk of developing FPD were similar among groups. MM administration increased breast meat yellowness and reduced amino acid digestibility (P < 0.001). The 1H-NMR analysis revealed variations in the levels of some circulating metabolites, including histidine, arginine and creatine, which play important metabolic roles. Overall, these findings can contribute to expand the knowledge about the use of Arthrospira spp. as protein source in broiler diets.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ
Good slaughter hygiene and accurate meat inspection in slaughterhouses (SHs) should ensure the hygienic quality of broiler chicken (broiler) carcasses accepted as food and guarantee that broiler meat ...from the carcasses is safe and fit for human consumption. The purpose of our research was to 1) determine the visible hygienic quality of broiler carcasses by visual inspection, 2) compare the results between the large Finnish SHs, and 3) determine the effectivity of the post-mortem inspection (PMI). We additionally aimed to study whether the requirement to inspect the body cavities of all carcasses during the PMI has had any impact on the visible hygienic quality of broiler carcasses in the SHs. The study was divided into two parts. In study 1, one researcher (the first author) inspected 2200 carcasses after PMI in three SHs and detected that, depending on the SH, 0.7–3.6% of carcasses had faecal contamination, 0.4–2.0% had gastrointestinal (GI) tract parts, 0.1–3.6% had some other form of contamination or other inedible remnants, and 0.2–0.5% showed pathological or organoleptic changes. Study 1 was carried out 2019, before the inspection of the body cavities of all carcasses was in use in PMI in the SHs.
One SH had significantly higher prevalences of carcasses with faecal contamination (P < 0.001 &P = 0.002) and residual GI tract parts (P = 0.004 &P = 0.006) than the other two SHs. The visual inspection in study 2 was performed 2020–2022 after the inspection of the external surfaces of all carcass body cavities had been included in PMI or after it was as a part of an own-check system. Only one SH had lower prevalences of carcasses with faecal contamination and residual GI tract parts (P = 0.009 &P = 0.005, respectively) in study 2 than in study 1. In all SHs, faeces were mostly found within the body cavities in both studies highlighting the importance of carcass body cavity inspection. A zero level of faecal contamination was not achieved in any SH. Assessing the visible hygienic quality of carcasses enables to assess the differences between SHs in slaughter hygiene management, enabling the risk categorization of SHs, along with assessing the efficiency of PMI.
•Visible faecal contamination was mostly within carcass body cavities.•A zero level of faecal contamination was not achieved in any of the slaughterhouses.•Local inflammation or soiling on carcasses may go unnoticed during post-mortem inspection.•Body cavity inspection is essential to find contamination and remaining visceral parts.•Process hygiene differences between slaughterhouses can be verified by visual carcass inspection.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ
•Nutritional value of Tenebrio molitor (TM) and Hermetia illucens (HI) was studied.•Total tract apparent digestibility of both TM and HI meal was determined.•Total tract apparent metabolizable energy ...of both TM and HI meal was determined.•Apparent ileal amino acid digestibility both TM and HI meal was determined.
The aim of this study was to determine the apparent digestibility coefficients of the total tract (CTTAD) of nutrients and the apparent metabolizable energy (AME and AMEn) of two insect larval meals (Tenebrio molitor and Hermetia illucens) for broiler chickens. The amino acid (AA) apparent ileal digestibility coefficients (AIDC) was also determined. The experimental diets were: a basal diet and two diets prepared by substituting 250g/kg (w/w) of the basal diet with Tenebrio molitor meal (TM) or Hermetia illucens meal (HI). No statistical difference was found between the two insect larval meals for the CTTAD of the nutrients, except for the CTTAD for ether extract (P<0.001) where the HI meal proved to be more digestible than the TM meal (0.99 and 0.88, respectively). The CTTAD for DM was 0.60 and 0.53; 0.66 and 0.66 for OM; 0.60 and 0.51 for CP, whereas it was 0.64 and 0.69 for GE, for TM and HI, respectively. No difference was observed between TM and HI (P>0.05) for AME or AMEn (AME=16.86 and 17.38MJ/kg DM, respectively; AMEn=16.02 and 16.60MJ/kg DM, respectively). The average AIDC of the 17 analyzed AAs was higher (P<0.001) in TM than in HI (0.86 and 0.68, respectively) because the AIDC of isoluecine, lysine, methionine, phenylalanine, valine, alanine, aspartic acid, glycine, glutamic acid and tyrosine was higher (P<0.05) in TM than in HI. Overall, the present results have shown that TM and HI meals are excellent sources of AME for broilers and a valuable source of digestible AA, particularly as far as TM meal is concerned.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Meat inspection of broiler chickens (broiler) in the European Union is regulated by common legislation to secure meat safety. However, the legislation is general in nature and proper post-mortem ...inspection (PMI) of every carcass and visceral organs of broilers is challenging in slaughterhouses (SHs) with a high slaughter line speed. The aim of this study was to investigate the on-site organization and possible differences of the PMI in four Finnish SHs, which slaughter over 99% of broilers in Finland. Our results show that the meat inspector's available inspection time per broiler in the PMI varied between 0.28 and 0.90 s, with the shortest available inspection time in the SH with the highest slaughter line speed and the longest available inspection time in the SH with the slowest line speed. We observed that only part of the total inspection time per broiler could be used for true PMI in most (3/4) SHs, as the meat inspectors also performed other tasks during the PMI. We observed deficiencies in the visual inspection of broiler carcasses; in particular, the proper inspection of all or most of the body cavities was impossible in all SHs during the PMI. Some deficiencies in facilities (e.g. in recording system) were observed. Moreover, lighting properties varied between the SHs and a significant difference between illumination conditions at the first inspection stations in the SHs was observed. This study considered the prerequisites for proper PMI and revealed that the PMI of broilers was not completely uniform in Finland. The results emphasize the need for more precise guidelines and recommendations, especially for inspection time and lighting at inspection stations.
•A short post-mortem inspection time may hamper detection of non-acceptable broilers.•Differences in facilities and lighting affected post-mortem inspection uniformity.•Measurements can be used to evaluate the uniformity of meat inspection stations.•The high line speed of slaughterhouses hinders proper inspection of body cavities.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ
Objective The objectives of this study were to evaluate the effects of daily feed intake during the laying period on embryonic myogenic differentiation 1 (MYOD1), myogenic factor 5 (MYF5), and ...myogenic factor 6 (MYF6) gene expression in genetically fat and lean lines of chickens. Methods An experiment in a 2×2 factorial design was conducted with two dietary intake levels (100% and 75% of nutrition recommendation) and two broiler chicken lines (fat and lean). Two lines of hens (n = 384 for each line) at 23th week of age were randomly divided into 4 treatments with 12 replicates of 16 birds. The experiment started at 27th week of age (5% egg rate) and ended at 54th week of age. Hatched eggs from the medium laying period were collected. Real time polymerase chain reaction analysis was used to analyse the MYOD1, MYF5, and MYF6 mRNA levels of E7, E9, E11, E13, and E15 body tissues and E17, E19, and E21 chest and thigh muscle samples. Results The results indicated that there were significant effects of line, dietary intake, and interactions between them on MYOD1, MYF5, and MYF6 gene mRNA expression levels in embryonic tissues. Low daily feed intake did not change the expression trend of MYOD1 mRNA in either line, but changed the peak values, especially in lean line. Low daily feed intake altered the trend in MYF5 mRNA expression level in both lines and apparently delayed its onset. There was no apparent effect of low daily feed intake on the trends of MYF6 mRNA expression levels in either line, but it significantly changed the values on many embryonic days. Conclusion Maternal nutrient restriction affects myogenesis and is manifested in the expression of embryonic MYOD1, MYF5, and MYF6 genes. Long term selection for fat deposition in broiler chickens changes the pattern and intensity of myogenesis.
ABSTRACT The research objective is to determine the determinants and impact of broiler chicken companies in East Nusa Tenggara. This research was conducted for 6 (six) months from September ...2018-February 2019 with the research locations in Kupang Regency and Kupang City which were determined by purposive sampling. The research method is descriptive employing surveys, observations, and interviews. The informants consisted of 8 (eight) people, namely Head of Animal Husbandry Office of Kupang Regency, Head of Animal Husbandry Field at the Agriculture Office of Kupang City, Researchers from the Undana Faculty of Animal Husbandry, and 5 (five) farmer who are members of the organization Himpuli (Himpunan Pengusaha Unggas Lokal Indonesia). The types of data used are primary data and secondary data. The data analysis used is descriptive which is described in a narrative. The results showed that NTT Province has the potential to establish broiler breeding companies with a concentration of development in Kupang City or Kupang Regency for Timor Island, Nagekeo Regency for Flores Island, and Southwest Sumba Regency for Sumba Island. The existence of broiler breeding companies in NTT will increase the effectiveness of the broiler marketing chain, stimulate new jobs, increase income supported by policies, socio-culture, economy, and distribution. This of course has been well studied from the aspect of overcoming environmental damage caused by the presence of broiler breeding companies in NTT. Keywords: Companies; Breeding; Broiler ABSTRAK Tujuan penelitian adalah untuk mengetahui faktor penentu dan dampak keberadaan perusahaan ayam ras pedaging di Nusa Tenggara Timur (NTT). Penelitian ini dilaksanakan selama 6 (enam) bulan dari bulan September 2018-Februari 2019 dengan lokasi penelitian di Kabupaten Kupang dan Kota Kupang yang ditentukan secara purposive sampling. Metode penelitian bersifat deskriptif dengan cara survei, observasi dan wawancara. Narasumber terdiri dari 8 (delapan) orang, yaitu Kepala Dinas Peternakan Kabupaten Kupang, Kepala Bidang Peternakan pada Dinas Pertanian Kota Kupang, Peneliti dari Fakultas Peternakan Undana, dan 5 (lima) pengusaha ayam ras pedaging yang tergabung dalam organisasi Himpuli (Himpunan Pengusaha Unggas Lokal Indonesia). Jenis data yang digunakan adalah data primer dan data sekunder. Analisis data yang digunakan adalah deskriptif yang dijabarkan secara naratif. Hasil penelitian menunjukkan bahwa Provinsi NTT berpotensi untuk didirikan perusahaan pembibitan ayam ras pedaging dengan konsentrasi pembangunan di Kota Kupang atau Kabupaten Kupang untuk wilayah Pulau Timor, Kabupaten Nagekeo untuk Pulau Flores, dan Kabupaten Sumba Barat Daya untuk kawasan Pulau Sumba. Adanya perusahaan pembibitas ayam ras pedaging di NTT akan meningkatkan efektifitas rantai pemasaran ayam ras pedaging, menstimulasi lapangan pekerjaan baru, meningkatkan pendapatan yang didukung oleh kebijakan, sosial budaya, ekonomi, dan distribusi. Hal tersebut tentu telah dikaji secara baik dari aspek penanggulangan kerusakan lingkungan yang ditimbulkan oleh adanya perusahaan pembibitan ayam ras pedaging di NTT. Kata kunci: Perusahaan; Pembibitan; Ayam ras pedaging
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