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A recently developed high-throughput background membrane imaging (BMI) technique, the HORIZON, was assessed for its ability to quantify subvisible particulate (SVP) generated during ...protein therapeutic development. The HORIZON platform method was optimized and compared to three well-characterized SVP counting techniques: light obscuration, micro-flow imaging (MFI), and FlowCam®. A head-to-head comparison was performed for precision, linearity, SVP concentration, and morphological output of BMI compared to the other three techniques using two unique enzymes under investigation. We found that dilution requirements for BMI are protein-specific, and membrane coverage is the critical instrument parameter to monitor for dilution suitability. The precision of BMI ranked similarly to all other techniques. Analysis of the same sample dilution, run in triplicate, across all four techniques indicated the BMI technique provides SVP concentrations that are comparable with the flow imaging techniques. Morphological information from BMI was generally less practical when compared with flow microscopy. The major drawback of BMI was that the current software indiscriminately clips large particles, potentially resulting in a misrepresentation of SVP size distribution. Despite this phenomenon, the concentration and size data generated corresponds well with current flow imaging techniques while decreasing time, cost, and sample requirements for SVP quantification.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
•The ZooCAM is an in-flow system for on-board imaging of fish eggs and zooplankton.•It enabled the analysis ~10,000 samples on-board in 4 years, without any failure.•It provides staged fish eggs ...counts equivalent to those done with microscopes.•It provides results comparable to the ZooScan on complex planktonic assemblages.•The ZooCAM helps improve the spatio-temporal resolution of zooplanktonic studies.
In this paper we present the ZooCAM, a system designed to digitize and analyse on board large volume samples of preserved and living metazooplankton (i.e. multicellular zooplankton) and fish eggs >300 µm ESD. The ZooCAM has been specifically designed to overcome the difficulties to analyse zooplankton and fish eggs in the framework of the PELGAS survey, and provide high frequency data. The ZooCAM fish eggs counts were comparable to those done with a dissecting microscope. The ZooCAM enabled the accurate prediction and fast on board validation of staged anchovy and sardine eggs in almost real time after collection. A comparison with the ZooScan, on a more complex zooplanktonic community, provided encouraging results on the agreement between the 2 instruments. ZooCAM and ZooScan enabled the identification of similar communities and produced similar total zooplankton abundances, size distributions, and size spectra slopes, when tested on the same samples. However these results need to be further refined due to the small number of samples used to compare the two instruments. The main ZooCAM drawback resides in a slight but sensible underestimation of abundances and sizes, and therefore individual and community biovolumes. The ZooCAM have been successfully deployed over 4 years, on numerous surveys without suffering any major failure. When used in line with the CUFES it provided high resolution maps of staged fish eggs and zooplanktonic functional groups. Hence the ZooCAM is an appropriate tool for the development of on board, high frequency, high spatial coverage zooplanktonic and ecosystemic studies.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Early warning systems monitoring the quality of drinking water need to distinguish between normal quality fluctuations and those caused by contaminants. Thus, to decrease the number of false positive ...events, normal water quality fluctuations, whether periodic or aperiodic, need to be characterized. For this, we used a novel flow-imaging particle counter, a light-scattering particle counter, and electrochemical sensors to monitor the drinking water quality of a pressure zone in a building complex for 109 days. Data were analyzed to determine the feasibility of the sensors and particle counters to distinguish periodic and aperiodic fluctuations from real-life contaminants. The concentrations of particles smaller than 10 μm and N, Small, Large, and B particles showed sudden changes recurring daily, likely due to the flow rate changes in the building complex. Conversely, the concentrations of larger than 10 μm particles and C particles, in addition to the responses of electrochemical sensors, remained in their low typical values despite flow rate changes. The aperiodic events, likely resulting from an abnormally high flow rate in the water mains due to maintenance, were detected using particle counters and electrochemical sensors. This study provides insights into choosing water quality sensors by showing that machine learning-based particle classes, such as B, C, F, and particles larger than 10 μm are promising in distinguishing contamination from aperiodic and periodic fluctuations while the use of other particle classes and electrochemical sensors may require dynamic baseline to decrease false positive events in an early warning system.
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•Periodicity in water consumption and water quality was studied.•A flow-imaging particle counter classified particles by machine learning.•Contamination event-specific particles are not detected in normal conditions.•Selection of suitable particle channels is an alternative to dynamic baseline.•Characteristics of artificial contamination vary from those of aperiodic events.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
•Three-dimensional Optical Coherence Tomography Angiography (OCTA) imaging of human eye anterior segment reported.•The system utilizes a 100 KHz A-scan rate with 1060 nm swept source OCT system.•In ...vivo depth-resolved volumetric imaging for microstructure & microvasculature visualization was performed.•Joint Spectral and Time domain OCT (STdOCT) method was employed to calculate direction of blood flow.•The vascular systems at limbus were imaged and is presented.
The study demonstrates in-vivo three-dimensional depth-resolved Optical Coherence Tomography Angiography (OCTA) and Doppler OCT imaging performed on the anterior segment of the human eye. A high-speed swept-source OCT system was developed utilizing a swept-source laser having a 100 kHz A-scan sweep rate and centre wavelength at 1060 nm. The proposed system has an axial resolution of 4.5 µm and a transverse imaging resolution of 14 µm. The Doppler OCT provides information regarding the direction of blood flow in corneoscleral junction vessels, which employs the Joint Spectral and Time domain OCT (STdOCT) method. Parameters influencing the imaging of various vessels, like scanning density, A-scan rate, B-scan averaging, and number of BM scanning repeats, were also studied. Slit lamp images and OCT angiography en-face projections produced from PVOCTA (Phase-variance OCTA) and STdOCTA from the same regular patients were compared in order to demonstrate the additional potential of the system. The vascular systems at the Limbus consisting of circulatory, lymphatic, and aqueous outflow systems were imaged and presented. The output clinical parameters of the system will contribute to understanding the blood circulation with directionality at the limbus location, which can be further utilized to identify glaucoma. In the author's opinion, this article represents the first attempt to provide Doppler OCTA data for the anterior chamber of the human eye.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Flow imaging microscopy (FIM) is widely used to characterize biopharmaceutical subvisible particles (SVPs). The segmentation threshold, which defines the boundary between the particle and the ...background based on pixel intensity, should be properly set for accurate SVP quantification. However, segmentation thresholds are often subjectively and empirically set, potentially leading to variations in measurements across instruments and operators. In the present study, we developed an objective method to optimize the FIM segmentation threshold using poly(methyl methacrylate) (PMMA) beads with a refractive index similar to that of biomolecules. Among several candidate particles that were evaluated, 2.5-µm PMMA beads were the most reliable in size and number, suggesting that the PMMA bead size analyzed by FIM could objectively be used to determine the segmentation threshold for SVP measurements. The PMMA bead concentrations measured by FIM were highly consistent with the indicative concentrations, whereas the PMMA bead size analyzed by FIM decreased with increasing segmentation threshold. The optimal segmentation threshold where the analyzed size was closest to the indicative size differed between an instrument with a black-and-white camera and that with a color camera. Inter-instrument differences in SVP concentrations in acid-stressed recombinant adeno-associated virus (AAV) and protein aggregates were successfully minimized by setting an optimized segmentation threshold specific to the instrument. These results reveal that PMMA beads can aid in determining a more appropriate segmentation threshold to evaluate biopharmaceutical SVPs using FIM.
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FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The capacity of today's gas‐liquid contacting equipment such as tray or packed columns is limited by the gravitational‐driven liquid flow. Intensified equipment applying centrifugal force offers ...great potential for enhancing the mass transfer and for reducing equipment size. Yet, detailed knowledge about the liquid flow inside rotating packings is scarce due to limited accessibility with conventional measurement systems. In this study, a gamma‐ray computed tomography is employed to quantify the liquid hold‐up and its distribution in the moving packing.
A non‐invasive CT‐based imaging technique was applied to an intensified gas‐liquid contactor. Due to synchronization of the CT system and the rotating packed bed, angle‐resolved reconstructions could be obtained. Furthermore, detailed evaluation of structural effects of packing and rotor internals on the liquid distribution could be revealed.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
While 2D phase-contrast MRI is often used to examine intracranial vessels in neurovascular disease contexts, the ability of 4D flow to assess many vessels at once makes it an attractive alternative. ...We aimed to assess the repeatability, reliability, and conformity of 2D and 4D flow across intracranial vessels.
Using correlation analyses and paired
-tests, test-retest repeatability, intra-rater reliability, and inter-method conformity for measurements of pulsatility index (PI) and mean flow were assessed in the arteries and veins of 11 healthy volunteers. Inter-method conformity was also assessed in 10 patients with small vessel disease.
Repeatability for PI measurements was mostly classed as good using both 2D (median ICC = 0.765) and 4D (0.772) methods, and for mean flow was mostly moderate across both (2D: 0.711, 4D: 0.571). 4D reliability was good for PI (0.877-0.906) and moderate for mean flow (0.459-0.723). Arterial PI measurements were generally higher using the 2D method, while mean flow was mostly higher using 4D flow.
These results imply that PI measurement using 4D flow is repeatable and reliable across intracranial arteries and veins, but care should be paid to absolute flow measurements as they are susceptible to variation depending on slice placement, resolution, and lumen segmentation practices.
Hematoporphyrin Monomethyl Ether (HMME) is a promising photosensitizer for photodynamic therapy (PDT) and has been widely applied in the treatment of port-wine stains (PWS).
Patients with PWS were ...treated with HMME-PDT, and efficacy was evaluated by comparing photographs and CDFI examination.
9 of the 33 cases were cured (27.27%), 10 indicated a good efficacy (30.30%), 11 showed alleviation (33.33%), while 3 had no obvious efficacy (9.09%). The efficacy was related to the types of lesions and the total number of therapeutic sessions. More sessions the purple type and nodular thickening type received, better clinical cosmetic results were. CDFI may be an excellent technique to evaluate efficacy.
HMME-PDT serves as a safe and effective approach for improving PWS. The clinical therapeutic effect is affected by the type of lesions and the total number of therapeutic sessions. CDFI examination may be an excellent evaluation method.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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Diagnostic ultrasound is non-invasive and provides real-time imaging. Microbubbles (MBs) are ultrasound contrast agents used to observe small blood flow, such as tumor tissue. ...However, MBs have short blood flow imaging time. This study developed lipid-based microbubbles (LMBs) with longer blood flow imaging time by focusing on their shell composition. Liposome research reported that addition 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG) to the lipid composition enhances liposome membrane stability. Therefore, we introduced DSPG at different ratios into the LMBs lipid shell. Results showed that the lipid shell composition of MBs affects stability in vivo. 60% DSPG-containing LMBs (DSPG60-LMBs) have sustained blood flow imaging time compared with LMBs, which have other DSPG ratios, Sonazoid® and SonoVue®. DSPG60-LMBs also showed less uptake into the liver compared with Sonazoid®. Therefore, DSPG60-LMBs can have long blood flow imaging time and can be effective diagnostic agents in ultrasound imaging.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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