Two new dipyrroloquinones, namely talaroterreusinones A (1) and B (2), together with four known secondary metabolites, terreusinone A (3), penicillixanthone A (4), isorhodoptilometrin (5), and ...chrysomutanin (6), were isolated from the solid culture of the endophytic fungus Talaromyces sp. by integrating mass spectrometry-based metabolic profiling and a bioassay-guided method. Their planar structures and stereochemistry were elucidated by comprehensive spectroscopic analysis including NMR and MS. The absolute configuration at C-1″ of terreusinone A (1) was established by applying the modified Mosher’s method. Compounds 1–6 were evaluated for anti-inflammatory activity and cytotoxicity. As a result, 1–3 inhibited the LPS-stimulated NO production in macrophage RAW264.7 cells, with ICsub.50 values of 20.3, 30.7, and 20.6 µM, respectively. Penicillixanthone A (4) exhibited potent cytotoxic activity against Hep G2 and A549 cell lines, with ICsub.50 values of 117 nM and 212 nM, respectively, and displayed significant antitumour effects in A549 cells by inhibiting the PI3K-Akt-mTOR signalling pathway.
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2.
Where I work Zgrablić, Željko
Nature (London),
06/2022, Volume:
606, Issue:
7914
Journal Article
Peer reviewed
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Four undescribed pyranone derivatives, named ascomycopyrones A–D (1–4), as well as one known analogue simplicilopyrone (5) (this is the first study to report the absolute configuration), were ...isolated from the endophytic fungus Ascomycota sp. FAE17 derived from the flowers of Scutellaria formosa. The structures of these pyranones were identified by comprehensive spectroscopic and MS analyses, and the absolute configurations were determined by their experimental and quantum chemical electronic circular dichroism (ECD) calculations. All isolated compounds were tested for various bioactivities, including antibacterial, cytotoxic activity, and NO inhibitory activity. Unfortunately, none of the compounds showed significant bioactivities.
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4.
Identification of Pathogenic Fungi Campbell, Colin; Johnson, Elizabeth; Warnock, David W
2013, 2013., 20130101, 2013-02-04, 2013-01-25
eBook
Since the first edition of Identification of Pathogenic Fungi, there has been incredible progress in the diagnosis, treatment and prevention of fungal diseases: new methods of diagnosis have been ...introduced, and new antifungal agents have been licensed for use. However, these developments have been offset by the emergence of resistance to several classes of drugs, and an increase in infections caused by fungi with innate resistance to one or more classes.Identification of Pathogenic Fungi, Second Edition, assists in the identification of over 100 of the most significant organisms of medical importance. Each chapter is arranged so that the descriptions for similar organisms may be found on adjacent pages. Differential diagnosis details are given for each organism on the basis of both colonial appearance and microscopic characteristics for the organisms described.In this fully updated second edition, a new chapter on the identification of fungi in histopathological sections and smears has been added, while colour illustrations of cultures and microscopic structures have been included, and high quality, four colour digital images are incorporated throughout.
Two undescribed p-terphenyl derivatives, asperterphenylcins A–B (1–2), and two undescribed diphenyl ether derivatives, asperdiphenylcins A–B (3–4), together with three previously described ...p-terphenyl derivatives—4″-deoxyterprenin (5), terphenyllin (6), and 3″-hydroxyterphenyllin (7)—were obtained from the solid-rice culture of the marine-derived fungus Aspergillus candidus HM5-4, which was isolated from sponges from the South China Sea. Their structures were elucidated by HRESIMS data and NMR spectroscopic analysis. Compound 1 showed a strong inhibitory effect on Neoscytalidium dimidiatum, with an inhibition circle diameter of 31.67 ± 2.36 mm at a concentration of 10.0 µg/disc. Compounds 5 and 7 displayed cytotoxic activity against human chronic myeloid leukemia cells (K562), human liver cancer cells (BEL-7402), human gastric cancer cells (SGC-7901), human non-small cell lung cancer cells (A549) and human HeLa cervical cancer cells, with ICsub.50 values ranging from 3.32 to 60.36 µM, respectively. Compounds 2, 6 and 7 showed potent inhibitory activity against α-glucosidase, with ICsub.50 values of 1.26 ± 0.19, 2.16 ± 0.44 and 13.22 ± 0.55 µM, respectively.
Biodiversity of Fungi Mueller, Greg M; Foster, Mercedes S; Bills, Gerald F
2004, 2004-12-31
eBook
Biodiversity of Fungi is essential for anyone collecting and/or monitoring any fungi. Fascinating and beautiful, fungi are vital components of nearly all ecosystems and impact human health and our ...economy in a myriad of ways. Standardized methods for documenting diversity and distribution have been lacking. A wealth of information, especially regrading sampling protocols, compiled by an international team of fungal biologists, make Biodiversity of Fungi an incredible and fundamental resource for the study of organismal biodiversity. Chapters cover everything from what is a fungus, to maintaining and organizing a permanent study collection with associated databases; from protocols for sampling slime molds to insect associated fungi; from fungi growing on and in animals and plants to mushrooms and truffles. The chapters are arranged both ecologically and by sampling method rather than by taxonomic group for ease of use. The information presented here is intended for everyone interested in fungi, anyone who needs tools to study them in nature including naturalists, land managers, ecologists, mycologists, and even citizen scientists and sophiscated amateurs. Covers all groups of fungi - from molds to mushrooms, even slime molds Describes sampling protocols for many groups of fungi Arranged by sampling method and ecology to coincide with users needs Beautifully illustrated to document the range of fungi treated and techniques discussed Natural history data are provided for each group of fungi to enable users to modify suggested protocols to meet their needs.
A deep understanding of the mechanism of fruiting body development is important for mushroom breeding and cultivation. Hydrophobins, small proteins exclusively secreted by fungi, have been proven to ...regulate the fruiting body development in many macro fungi. In this study, the hydrophobin gene Cmhyd4 was revealed to negatively regulate the fruiting body development in Cordyceps militaris, a famous edible and medicinal mushroom. Neither the overexpression nor the deletion of Cmhyd4 affected the mycelial growth rate, the hydrophobicity of the mycelia and conidia, or the conidial virulence on silkworm pupae. There was also no difference between the micromorphology of the hyphae and conidia in WT and ΔCmhyd4 strains observed by SEM. However, the ΔCmhyd4 strain showed thicker aerial mycelia in darkness and quicker growth rates under abiotic stress than the WT strain. The deletion of Cmhyd4 could promote conidia production and increase the contents of carotenoid and adenosine. The biological efficiency of the fruiting body was remarkably increased in the ΔCmhyd4 strain compared with the WT strain by improving the fruiting body density, not the height. It was indicated that Cmhyd4 played a negative role in fruiting body development. These results revealed that the diverse negative roles and regulatory effects of Cmhyd4 were totally different from those of Cmhyd1 in C. militaris and provided insights into the developmental regulatory mechanism of C. militaris and candidate genes for C. militaris strain breeding.
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Five new isocoumarins, phaeosphaerins A–E (1–5), were isolated from the fermentation broth of the marine fungus Phaeosphaeriopsis sp. WP-26, along with one known isocoumarin, ...6,8-dihydroxy-7-methoxy-3-methylisocoumarin (6), and two known pimarane-type diterpenes, diaportheins A (7) and B (8). Their structures were elucidated via NMR experiments, X-ray diffraction analysis, and comparison of the experimental and computed ECD curves. Compounds 1–7 displayed weak neuroprotective effects against Hsub.2Osub.2-induced damage in SH-SY5Y cells. Moreover, compound 8 showed cytotoxicity against BEL-7402, SGC-7901, K562, A549, and HL-60 cell lines.
We investigated the sorption of Pu(IV) on biogenic Mn oxide, composed of Mn(IV) oxide and hyphae, produced by Mn(II)-oxidizing fungus. The sorption of Pu(IV) on biogenic Mn oxide was similar to that ...of U(VI) and different from that of Th(IV), possibly due to oxidation of Pu(IV) to Pu(VI). When Pu(IV) was sorbed on hyphae only, it was desorbed into the solution phase over time. Pu(IV) could be solubilized by complexation with organic ligands secreted by fungal cells. Furthermore, Pu(IV) desorption was observed especially under circumneutral pH conditions, indicating that Pu(IV) can be solubilized by microbial activity in most surface environments.
