•The first report of catalase produced by thermophilic bacteria Ureibacillus spp. strains.•The yield of catalase was the highest level among wild strains reported so far.•Stable at high temperature ...and alkaline conditions made it suitable for textile and other industries.•A novel catalase with low similarity with other reported catalase on amino acid sequences.
A catalase-producing thermophilic bacterium, Ureibacillus thermosphaericus FZSF03, was isolated from high-temperature compost. Catalase production in this strain increased 31 times and reached 57,630U/mL after optimization in a shake flask, which might represent the highest catalase activity level among reported wild strains. This catalase was further purified and identified. The purified enzyme showed a specific activity of 219,360U/mg, higher than many other catalases. The molecular weight of this enzyme is 52kDa according to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the enzyme was identified as a monofunctional haeme catalase of Ureibacillus thermosphaericus by liquid chromatography-mass spectrometry (LC–MS)/MS. The optimal reaction temperature for this catalase was found to be 60°C. Stability was observed at 60°C and at a pH of 10.0, indicating the superiority of this enzyme at a high temperature and under alkaline conditions. Therefore, this catalase is a prospective candidate for industrial production and applications. The gene encoding this catalase is 1503bp. As the amino acid sequence shows low similarity with other catalases, we suggest that this is a novel monofunctional haeme catalase.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Prolactin is a pituitary hormone that is involved diverse physiological functions, such as lactation, reproduction, metabolism, osmoregulation, immunoregulation, and behavior. Its level of ...glycosylation is low in vivo, which favors its expression in bacterial systems. In the present work recombinant human prolactin (rec-hPRL) was expressed from the p1813-hPRL vector in Escherichia coli strain in inclusion bodies with 530.67 mg of rec-hPRL per liter of induced bacterial culture. The solubilization and renaturation of rec-hPRL followed by two methods described in the literature for this protein: one with detergent and basic pH, and other urea and dialyses was done by studying. The protocol with detergent/basic pH was not successful, whereas protocol with urea/dialyses was obtained pure protein and this was optimized. Rec-hPRL was obtained in a soluble, pure and active form, when the sample was 8-fold concentrated in the solubilization phase, allowing 33% recovery, 3-fold more that the original method. The pure protein was obtained with 38.37 i. u./mg activity, which is three times greater than that of the PRL standard from the WHO. In conclusion, this work obtained the highest production of rec-hPRL, and concentrating the sample eight times in the solubilization stage was decisive for obtaining a highly concentrated, active protein for future work.
•The synthesis of rec-hPRL by p1813-hPRL in the HB2151 strain was 531 mg/L.•The strategy of concentrating the sample 8-folds before of the purification had an excellent result, with 33% recovery.•The concentration of the sample was an important step in obtaining the rec-hPRL mainly in the monomeric form. .•The pure protein was obtained with 38.37 i. u./mg activity, 1.8 times more active than the WHO standard.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Since the approval to establishment, the Changning-Weiyuan National Shale Gas Demonstration Area in the Sichuan Basin (hereinafter referred to as Demonstration Area) has been built up into a shale ...gas industry demonstration base characterized with scale, technology, management and green after more than 10 years of exploration and practice. Its successful experience is of great guiding significance to the efficient development of unconventional oil and gas resources in China. In this paper, the technologies which were developed for the beneficial development of the Demonstration Area during the 13th Five-Year Plan are systematically reviewed and summarized. Then, some countermeasures are proposed to solve the difficulties and challenges to the long-term benefit and stable production of middle-deep shale gas in this area. And the following research results are obtained. First, during the construction of the Demonstration Area, the “three-control” shale gas enrichment and high-production theory is innovatively proposed, and the shale gas enrichment model in the southern Sichuan Basin is clarified. Second, a high-production well cultivation method based on geology-engineering integration is established by developing six main technologies for shale gas exploration and development, which effectively supports the large-scale beneficial development of shale gas in the Demonstration Area. Third, the beneficial development model of middle-deep shale gas in the southern Sichuan Basin is preliminarily formed, and the benefit and efficiency of productivity construction are improved comprehensively. Fourth, the three measures of “controlling the decline rate, enhancing the reserve production and expanding the periphery” are used to support the replacement and stable production of middle-deep shale gas. In conclusion, to sustainably ensure the long-term benefit and stable production of shale gas in the southern Sichuan Basin, it is still necessary to deepen fine reservoir description and complex mechanism understanding and explore EOR technologies. What's more, the important mean to improve the single-well shale gas production rate in this area is to strengthen the fine fracturing design and implementation under the deep integration of geology and engineering and improve the construction quality of fracturing network continuously. Furthermore, the only way to the low-cost development of the demonstration area is to promote the management reform and innovation continuously and improve the beneficial development model further.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Based on observation of cores and casting thin sections, reservoir fluorescence, hydrocarbon geochemical characteristics and oil production test data, the factors controlling the tight oil ...accumulation and high production in the Da'anzhai Member in the Jurassic Ziliujing Formation in central Sichuan Basin are studied. The shelly limestone, muddy shell limestone and tight limestone are all oil-bearing in the Da'anzhai Member. The dissolved pores and fractures in shelly limestone and the matrix pores (microfractures, intercrystal pores) in tight limestone are all oil-bearing in large area, which is the reason why oil wells can keep low production for a long period of time. On the basis of the extensive oil-bearing, natural gas from the Upper Triassic Xujiahe Formation provided the major power for oil migration and accumulation in the Da'anzhai low amplitude, water-free tight limestone reservoirs, driving the dispersed oil from the matrix pores into an accumulation, so the oil is more enriched in the western structures of central Sichuan Basin where oil wells are higher in gas-oil ratio. In contrast, in the east of central Sichuan, the Huayingshan major faults generally cut through Da'anzhai Member, allowing gas to migrate to the formations above Da'anzhai Member along fractures to accumulate or dissipate, without gas as driving force, as a result, the oil is less enriched than that in the west part of central Sichuan.
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A mulched drip fertigation system is an effective way to improve maize yield, but at present, the efficiency of nutrient delivery and water use are generally low. Therefore, this study conducted ...optimization field experiments to identify the main factors limiting the delivery of water and fertilizer, including regulations on nitrogen (N) fertilizer, irrigation conditions, planting density and maize varieties, in the semi-arid area of Northeast China. As part of an effort to close the yield gap for maize, an optimized system (DS) for optimal crop, nutrient and water management was designed to improve the agronomic and economic performance of maize farming in the area. The application rate of N fertilizer was 240 kg ha−1; the base fertilizer was applied four times (once at the jointing stage, twice at the belling stage and once at the silking stage); the rates of application of phosphorus (P) and potassium (K) fertilizer were 90 kg P2O5 ha−1 and 90 kg K2O ha−1, respectively; the irrigation amount was 270 mm ha−1; the maize variety Fumin 985 was planted at a density of 80,000 plants ha−1 in DS; the grain yield of DS reached 13.8 Mg ha−1, 93% of the yield potential. DS yielded an economic benefit of 18,449 yuan ha−1, which was significantly higher than the economic benefit of 13,818 yuan ha−1 achieved under farmers’ practices (FP). Furthermore, the utilization rates of N, P, K, and water were significantly improved under DS. In conclusion, DS increased production potential, with high efficiency in nutrient delivery and water use and low losses of nutrients and water. The crop, fertilizer, and water management of DS provided a technological system to simultaneously improve crop production and resource-use efficiency in the semi-arid area of Northeast China.
The aim of this study was to develop a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)-based method for the multiresidue analysis of 21 plastic additives in river water. ...Analysed compounds included phthalates, benzophenone, bisphenol A and long- and short-chain alkylphenols (APs), which are of relevance because most of them are high production volume chemicals largely used in the plastic industry. These compounds can reach river waters due to direct discharge from wastewater treatment plants and leaching from plastic materials and microplastics present in rivers. In a first step, ionisation and acquisition parameters were optimised to obtain high sensitivity and structural information. Then, different solid-phase extraction cartridges and elution conditions were tested using Milli-Q and river water. With the optimised conditions, quality control parameters (recoveries, limits of detection, intra- and inter-day variability and blank contribution) proved that the method was accurate and selective for the trace monitoring of these compounds in river water. For nonylphenol, octylphenol and bis(2-ethylhexyl)phthalate, included in the Water Framework Directive, limits of detection were below environmental quality standard concentrations considering blank contributions. All other compounds were efficiently detected at trace levels, and focus was given to
o
- and
p
-substituted and di- and tri-substituted APs, which are first reported in the present study. A pilot survey was finally carried out to determine the occurrence of plastic additives in river waters surrounding the city of Barcelona.
Abstract
Soybean consumption (Glycine max L.) in Indonesia has increased significantly. The very large demand for soybeans and limited ability to import demand the immediate development of domestic ...soybeans. One of the inhibitors that can reduce soybean production is a disease caused by Athelia rolfsii (Curzi). The development program needs to be supported by plant breeding as an effort to overcome problems in developing soybean cultivation technology, one of which is by assembling high-yielding varieties that are always accompanied by disease resistant characters. This study was aimed to determine the level of resistance to stem rot disease of A.rolfsii Curzi and production of soybean (M7) mutan genotype under disease stress and optimum condition. This research was conducted in 2019 using an augmented design. The treatments used were 11 mutant genotypes and 3 comparison varieties, anjasmoro variety, argomulyo variety, and kipas putih variety. The appearance of agronomic characters observed in agronomic traits on inoculated area by A.rolfsii Curzi is lower than the optimum condition. Based on the plant sensitivity index, genotype M100A25(3/7) and M200A12(6/5) had resistant of stem rot disease while M100A25(5/3), M100A25(3/4), M100A6(31/1), M200A11(32/3) had moderate resistance to stem rot disease. The result of strains selection on M7 generation resulted in two strain with high production character and resistance to stem rot disease, M100A25 (3/7) and M200A12(6/5).
This paper summarizes the environmental hazard assessment of physicochemical properties, environmental fate and behavior and the ecotoxicity of a category of 61 anionic surfactants (ANS), comprised ...of alkyl sulfates (AS), primary alkane sulfonates (PAS) and alpha-olefin sulfonates (AOS) under the High Production Volume Chemicals Program of the Organisation for Economic Co-operation and Development (OECD).
The most important common structural feature of the category members examined here is the presence of a predominantly linear aliphatic hydrocarbon chain with a polar sulfate or sulfonate group, neutralized with a counter-ion.
The hydrophobic hydrocarbon chain (with a length between C
8 and C
18) and the polar sulfate or sulfonate groups confer surfactant properties and enable the commercial use of these substances as anionic surfactants. The close structural similarities lead to physico-chemical properties and environmental fate characteristics which follow a regular pattern and justify the applied read-across within a category approach. Common physical and/or biological properties result in structurally similar breakdown products and are, together with the surfactant properties, responsible for similar environmental behavior. The structural similarities result in the same mode of ecotoxic action. Within each of the three sub-categories of ANS the most important parameter influencing ecotoxicity is the varying length of the alkyl chain. Although the counter-ion may also influence the physico-chemical properties, there is no indication that it significantly affects chemical reactivity, environmental fate and behavior or ecotoxicity of these chemicals.
Deduced from physico-chemical and surfactancy properties, the main target compartment for the substances of the ANS category is the hydrosphere. They are quantitatively removed in waste water treatment plants, mainly by biodegradation. Quantitative removal in biological treatment plants is reflected by low AS concentrations measured in effluents of waste water treatment plants (mostly below 10
μg/L). In addition, bioaccumulation of ANS does not exceed regulatory triggers based upon experimental data.
A considerable number of reliable aquatic toxicity data for the whole ANS category are available, including chronic and subchronic data for species of all trophic levels.
Based upon the highest quality data in hand, there appears to be no singularly most sensitive trophic level in tests on the toxicity of alkyl sulfates, with a large degree of overlap among algae, invertebrates and fish. Algae proved to be more variable in sensitivity to alkyl sulfate exposure compared to fish and daphnia. The key study for the aquatic hazard assessment is a chronic test on
Ceriodaphnia dubia, which covers a range of the alkyl chain length from C
12 to C
18. A parabolic response was observed, with the C
14 chain length being the most toxic (7
d-NOEC=0.045
mg/L). Responses of aquatic communities to C
12 AS and C
14–15 AS have been studied in high quality stream mesocosm studies containing a broad range of species and ecological interactions. These studies are regarded as a better approximation to reality when extrapolating to the environment. The 56-d chronic NOEC for C
12 AS and C
14–15 AS were 0.224 and 0.106
mg/L, respectively, based on integrated assessments of periphyton (algal, bacterial and protozoan) and invertebrate communities.
Taking into account the rapid biodegradation of the ANS compounds as well as the low concentrations measured in different environmental compartments, this category of surfactants is of low concern for the environment.
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With high rates of food and nonrenewable fossil fuel consumption worldwide, we are facing great challenges in ensuring food and energy security to satisfy the world population. Intercropping, as an ...important and sustainable cropping practice in agroecosystems, has been widely practiced around the world. Many studies have shown that some plants can deliver high yields when intercropped with other plants. Here, we review the biological mechanisms in improving resource utilization efficiency and illustrate the practical application of intercropping in ensuring food and energy security through improving production. Identifying suitable energy plants for marginal land, land not suitable for food crops growth, is an effective strategy to acquire high production of bioenergy, thus removing competition between the use of land for food and energy. The effective application of intercropping provides a potential pathway for production of food crops and energy plants by improving resource use efficiency and resistance to environmental stress.
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