Cage-free housing of laying hens may provide opportunities for widespread environmental distribution of Salmonella contamination and horizontal transmission of infection within flocks. Salmonella ...Enteritidis in commercial laying flocks presents an ongoing public health concern because reproductive organ colonization in hens leads to deposition inside eggs. Many S. Enteritidis control programs include vaccination to induce protective immunity against infection. Salmonella Kentucky is common in egg production environments but has not been associated with egg contamination. This study compared the invasion of internal organs and horizontal spread of infection during the first 2 wk after experimental S. Enteritidis and S. Kentucky infection of previously vaccinated laying hens in indoor cage-free housing. Two groups of 72 hens each were housed in isolation rooms simulating commercial cage-free barns and 1/3 of the hens were orally inoculated with either S. Enteritidis (1 room) or S. Kentucky (1 room). At 6 and 12 d after inoculation, half of the hens in each room were euthanized and samples of the liver, spleen, ovary, oviduct, and intestinal tract were removed for bacteriologic culturing. Among hens inoculated with S. Enteritidis, 66.7% of the intestinal, liver, and spleen samples were positive for the pathogen at 6 d after infection, as well as 41.7% of intestines and 16.7% of livers from contact-exposed hens. Significantly (P < 0.05) fewer hens were colonized by S. Kentucky. These results demonstrate that vaccines may not always provide complete exclusion of Salmonella. In cage-free housing systems, vaccination should be supplemented with a comprehensive risk reduction effort to prevent extensive horizontal dissemination of Salmonella.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPUK, ZAGLJ, ZRSKP
Disease outbreaks can dramatically impact a producer's production schedule by disrupting the pullet movement into the hen house. One alternative is to early molt laying hens as they are coming into ...production. Early molted birds had lower production compared to controls with no differences in egg weight or egg components percentages. Bird welfare impacts were minimal as a result of the early molt. This management practice could be employed to allow pullets to be held minimizing production schedule disruptions as a result of a disease outbreak.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPUK, ZAGLJ, ZRSKP
Investigate the efficacy of Bacillus-based probiotics supplemented at two different levels to modulate the productive performance, egg quality, tibia traits, and specific cecal bacteria counts of ...Hy-Line Brown layers from 25 to 37 weeks of age.
A total of 216 twenty-five-week-old hens were randomly distributed into 3 experimental diets with 12 replicates of 6 birds per cage. Diets included basal diet supplemented with 0 (CON), 3 × 108 (PRO1), or 3 × 109 (PRO2) CFU of the test probiotic containing Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 per kilogram of feed.
Improved egg weights and mass at 29 weeks; and feed intake at 31 weeks (p < 0.10) were noticed with the probiotic-supplemented PRO1 and PRO2 diets. Considering egg quality, the shell thickness, Haugh units, and yolk color were improved; but yolk cholesterol was lowered (p < 0.05) with PRO1 and PRO2 diets at 29 weeks. At both 33 and 37 weeks, the egg-breaking strength, shell color and thickness, albumen height, Haugh units, and yolk color were improved; but yolk cholesterol was similarly lowered (p < 0.05) with the PRO1 and PRO2 diets. Improved tibia Ca, ash, weights, and density; and raised cecal counts of Bifidobacteria and Lactobacilli (p < 0.05) were noticed with PRO1 and PRO2 diets. Improved tibia P but reduced Clostridia counts (p < 0.10) were also observed with the PRO1 and PRO2 diets.
Probiotic supplementation of Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 at 3 ×108 CFU/kg of feed is adequate to significantly improve egg quality, lower yolk cholesterol, enhance several tibia traits, and raise the populations of beneficial cecal bacteria. Modest improvements in several productive parameters and tibia P but reduced Clostridia were also observed; and could warrant further investigation of probiotic effects beyond the current test period.
The influence of the main cereal and type of supplemental fat in the diet on productive performance and egg quality of the eggs was studied in 756 brown-egg laying hens from 22 to 54 wk of age. The ...experiment was conducted as a completely randomized design with 9 treatments arranged factorially, with 3 cereals (dented corn, soft wheat, and barley) and 3 types of fat (soy oil, acidulated vegetable soapstocks, and lard). Each treatment was replicated 4 times (21 hens/replicate). All diets were formulated to have similar nutrient content, except for linoleic acid, which ranged from 0.8 to 3.4% depending on the combination of cereal and fat source used. This approach allows for the estimation of the minimum level of linoleic acid in the diets that maximizes egg weight. Productive performance and egg-quality traits were recorded every 28 d, and the BW of the hens was measured individually at the beginning and at the end of the experiment. No significant interactions between main factors were detected for any of the variables studied. Egg production, egg weight, and egg mass were not affected by dietary treatment. Body weight gain was higher (P < 0.05) for hens fed corn or wheat than for hens fed barley, and also higher for hens fed lard than for hens fed soy oil or acidulated vegetable soapstocks. Egg quality was not influenced by dietary treatment, except for yolk color, which was greater (P < 0.001) for hens fed corn than for hens fed wheat or barley, and greater for hens fed lard than for hens fed soy oil or acidulated vegetable soapstocks. We concluded that brown-egg laying hens do not need more than 1.0% of linoleic acid in their diet (1.16 g/hen per d) to maximize egg production and egg size. The 3 cereals and the 3 fat sources tested can replace each other in the diet provided that the linoleic acid requirements to maximize egg size are met.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPUK, ZAGLJ, ZRSKP
This study aimed to determine the health status of laying hens using a combination of magnetic technology and probiotics in drinking water. A total of two hundred and eighty-eight (288), 57-week-old ...ISA Brown laying hens were assigned to six treatments with nine replicates in drinking water. Statistical data analysis followed a completely randomized design with treatments including water as control, magnetic technology (MT), 0.6% probiotic (PRO), 0.6% probiotic and magnetic technology (PRO+MT), 0.6% encapsulated probiotic (EPRO), and 0.6% encapsulated probiotic and magnetic technology (EPRO+MT). The laying hens received treatments for six weeks (42 d). The encapsulated probiotic was used to improve probiotic durability from intestinal damage. The health status consisted of blood and immunity profiles, including leukocytes, erythrocytes, haemoglobin, and haematocrit, CD8+, CD4+, malondialdehyde, and B cells. The water quality was improved by the use of magnetic technology. Magnetic technology effectively decreased the CD8+ , CD4+ , malondialdehyde, and B cells in the blood of laying hens compared to treatments without magnetic technology. The combination of magnetic and encapsulation technology showed maximum results in improving blood profile and immune response.
Full text
Available for:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
This study aimed to determine the effects of Arginine silicate inositol complex (ASI; Arg=49.47 %, silicone=8.2 %, inositol=25%) supplementation on egg quality, shell strength, and blood biochemical ...traits of laying hens, as well as the effects of substituting inositol with varying concentrations of phytase on the traits as mentioned above. 90 Lohmann Brown laying hens, 26 weeks old, were randomly distributed in 6 treatments with 3 replicates (cage) and 5 birds per replicate. The isocaloric and isonitrogenic diets are used according to the age period requirements of the Lohmann Brown Classic management guideline. The treatments were as follows: 1
treatment T1: received basal diet without additives, T2 received basal diet +1000 mg/kg arginine-silicate mixture (49.5±8.2 % respectively), T3 received basal diet +1000 mg/kg arginine-silicate- inositol (ASI) mixture (49.5, 8.2 , 25 % respectively), T4 received basal diet +1000 mg/kg arginine-silicate mixture (49.5±8.2% respectively) +500 FTU/kg, T5 received basal diet +1000 mg/kg arginine-silicate mixture (49.5±8.2% respectively) +1000 FTU/kg and T6 received basal diet +1000 mg/kg arginine-silicate mixture (49.5±8.2% respectively) +1000 FTU/kg +2000 FTU/kg. Results indicate a significant increase (
<0.05) in the relative yolk weight in T4, T5, and T6 (26.93, 26.83, 26.77%) compared to T1 (25.84%) and a significant increase (
≤ 0.05) in T4, T5 compared to T3 (26.02%), while no differences observed between T2 (26.17%) compared to other experimental treatments. The relative albumin weight significantly decreased (
≤0.05) in phytase supplementation treatments T4, T5, and T6 (63.21, 63.05, 63.22%) compared to T1, T2, T3 (64.99, 64.30, 64.08%), while a significant decrease (
≤0.05) observed in T3 compared to T1. The relative shell weight significantly increased (
≤0.05) in T3, T4, T5, and T6 (9.90, 9.86, 10.12, 10.02%), respectively, compared to T1, T2 (9.17, 9.53%) with a significant increase (
≤0.05) in relative shell weight in T2 compared to T1. The eggshell thickness significantly increased (
≤0.05) in T3, T4, T5, T6 treatments (0.409, 0.408, 0.411, 0.413 mm), respectively compared to T1, T2 (0.384, 0.391 mm). A significant increased (
≤0.05) was observed in eggshell thickness in T2 compared to T1. A significant increase (
≤0.05) was observed in the egg shell breaking strength in T3 and T5 treatments (59.40, 58.83) compared to T1 and T2 (46.20, 48.23). No significant differences were observed between T4 and T6 (53.90, 53.57) compared to other experimental treatments. Non HDL, calcium, and phosphorus levels in blood serum significantly increased (
≤0.05) in T3, T4, T5, and T6 treatments compared to T1 and T2 treatments.
In the present study, 200 Brown commercial egg-type layers (60 wk old) were used to study the effects of different levels of ecofriendly synthesis of calcium (Ca) nanoparticles (0.0, 0.50, 1.0, and ...1.5 g/kg diet) with biocompatible Sargassum latifolium algae extract (SL-CaNps) on exterior egg quality traits, electronic microscopic view of eggshells, Ca and phosphorus (P) retention, serum Ca and P concentrations, and the histology of the uterus. Hens fed with dietary SL-CaNps powder had higher egg weight and shell weight % values than those of the control group. All SL-CaNps treatment groups had the greatest values of shell weight per unit surface area and shell thickness. Dietary supplementation of SL-CaNps at graded levels up to 1.5 g/kg diet had higher serum Ca and inorganic P levels than that of the control. Laying hens fed with SL-CaNps-added diets had beneficial effects on shell ultrastructure in terms of well-developed palisade and mammillary layers. The numbers of apical cells along the branched tubular gland were greater in SL-CaNps-treated groups than those of control. Conclusively, supplementing SL-CaNps powder up to 1.5 g/kg to the diet of laying hens improved eggshell thickness, shell weight% and shell weight per unit surface and has no adverse effect on their eggshell quality or electronic microscopic view of their eggshell.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPUK, ZAGLJ, ZRSKP