Thymus vulgaris L. is found all over the world and is cultivated in several countries. It is considered an important medicinal plant with anti-inflammatory and aromatic properties. Its cultivation ...can suffer from infestation by numerous pathogens, which contributes to lower production. Thus, in 2020, a nematological survey in a vegetable growing area in Jaboticabal, São Paulo, Brazil, detected the presence of galls on the root, indicating that it was Meloidogyne spp. To identify the species, a sample with soil and roots was sent to the laboratory. After the analyzes performed, the species was identified as Meloidogyne javanica. This result was based on the morphological characteristics of the adults and the genetic identification. In the morphological part, the following characteristics were found: Perineal region of females low trapezoidal dorsal arch with two lines in laterals, while males have broader basal nodules with a non-raised labial disk, with the head region not separated from the body. Molecular confirmation was performed by genetic sequencing and sequence characterized amplified regions technique (SCAR). This is the first report of T. vulgaris as a host for M. javanica confirmed by Koch's postulate and several lines of evidence. Based on this report, farmers wishing to grow this vegetable should be aware of plants that are also hosts for this species.
Display omitted
•Meloidogyne javanica was observed on Thymus vulgaris in São Paulo, Brazil.•This is the first report of T. vulgaris as a host for M. javanica.•Thymus vulgaris L. (Lamiaceae) is considered an important medicinal plant.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
At least 1–2% of DNA sequences annotated as Blastocystis in GenBank represent organisms other than Blastocystis or sequence artefacts. As well as being biologically incorrect, such practice can lead ...to overestimates of genetic diversity, underestimated host specificity, and incorrect classification of samples tested for Blastocystis using DNA-based methods.
At least 1–2% of DNA sequences annotated as Blastocystis in GenBank represent organisms other than Blastocystis or sequence artefacts. As well as being biologically incorrect, such practice can lead to overestimates of genetic diversity, underestimated host specificity, and incorrect classification of samples tested for Blastocystis using DNA-based methods.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
There is few information regarding the identification of filamentous fungi present in sewage sludge, the importance of these microorganisms lies in the potential use they have in the food, ...pharmaceutical and agri-environmental industries as they are beneficial in the remediation of water, sludge and soil contaminated by Heavy metals and hydrocarbons also contribute to the adsorption of nutrients by crops. The use of residual sludge once decontaminated by these microorganisms can be used in agriculture as soil structure improvers, organic fertilizers and as an amendment to prevent soil erosion. The objective of this research was to characterize and identify fast and medium-growing filamentous fungi from sewage sludge. The residual sludge was collected directly from the thickness of the municipal water treatment plant of Cd. Victoria, Tamaulipas, Mexico. Subsequently, the fungi were isolated on agar plates and identified using morphological characters of the colonies and by PCR and diana sequences. The species identified were: Trichoderma longibrachiatum, Aspergillus terreus, Tylopilus porphyrosporus and Aspergillus fumigatus. Being the first report for Mexico of fungal species that grow in residual sludge from water treatment plants. The molecular characterization of the species of filamentous fungi found in sewage sludge will allow progress in research focused on knowing the advantages and biological and chemical mechanisms of these microorganisms for the development of biotechnological processes and products, with high agricultural potential and environmental.
Trichinella spiralis was long considered the sole Trichinella species in Argentina. However, since 2004, various Trichinella species, including the encapsulated Trichinella patagoniensis and ...Trichinella britovi, as well as the unencapsulated Trichinella pseudospiralis, have been detected in the country. The present study aimed to identify Trichinella ML at the species level from cougars naturally infected from Argentina. To this end, muscle tissue samples from one cougar each from Córdoba, Neuquén, and Santa Cruz Provinces were individually analysed using the artificial digestion technique. DNA extraction and molecular identification of Trichinella species were conducted on individual muscle larvae by PCR amplification of the ESV region and subsequent PCR amplification and sequencing of the COI gene. Morphological analysis revealed muscle larvae with characteristics consistent with Trichinella genus. PCR revealed a single band of approximately 127 bp for each individual muscle larva. PCR amplification of the COI gene from each isolate generated a 309 bp band. Sequencing of the mitochondrial COI gene confirmed the identity of the parasite as T. patagoniensis. The present study documents new occurrences of T. patagoniensis in Puma concolor from Argentina, including the first detection of T. patagoniensis in Puma concolor from Córdoba and Neuquén Province. These findings expand the limited knowledge of T. patagoniensis distribution in Argentina.
Display omitted
•Novel occurrences of T. patagoniensis identified in Argentina.•Isolation of T. patagoniensis documented in three cougar specimens.•First report of T. patagoniensis presence in Neuquén and Córdoba provinces.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
A combined study of morphology, stem anatomy, and the genetic markers (ITS1-ITS4 region of nuclear ribosomal DNA and rpl20-rps12 intergenic spacer of chloroplast DNA) was used to verify the identity ...of hybrid Potamogeton ×vilnensis Galinis previously recognized as P. gramineus × P. praelongus. Authentic herbarium material and newly collected specimens morphologically similar to the hybrid from the locus classicus (SE Lithuania) were used for comparative morphological, anatomical and molecular analyses. The study proved the paternity of P. lucens and P. praelongus and excluded the involvement of P. gramineus in hybridization and identity with P. ×angustifolius. This hybrid formula is shared with plants recorded in the Czech Republic and Denmark under the name P. ×jutlandicus Zalewska-Galosz. The site in Lithuania, known since 1961, is the first natural habitat of long-lived hybrid between P. lucens and P. praelongus whose origin has been confirmed by molecular markers. The scarce records of this taxon indicate that the knowledge of the hybrid species of Potamogeton and their distribution is insufficient and that more extensive regional studies, including molecular methods, are needed.
•Potamogeton ×vilnensis Galinis was originally introduced as P. gramineus × P. praelongus.•Potamogeton lucens and P. praelongus parentage confirmed for P. ×vilnensis.•Hybrid formula for naturally long-living plants was first evidenced by molecular methods.•Genetic markers (ITS1-ITS4 and plastid rpl20-rps12 regions) were used for hybrid verification.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Pitaya (Hylocereus spp.) is a cactus cultivated in regions with a tropical and subtropical climate. In Brazil, the installation of pitaya orchards has spread nationwide, and some diseases associated ...with phytopathogens have been reported. In Pará, a state located in the North of Brazil, the constant association of a fungus in pitayas, characteristic of the genus Neoscytalidium, was observed, manifesting symptoms of stem canker. Therefore, this study aimed to identify and confirm the causal agent associated with stem canker in pitayas (H. costaricensis) in Pará, Brazil. Symptomatic pitaya cladodes were used to isolate the pathogens. Subsequently, the macroscopic and microscopic characteristics of the colonies, molecular analysis, and pathogenicity test were carried out. The pathogen showed rapid growth on PDA, with a diameter of approximately 8–9 cm, after 4–5 days of incubation at 25 °C with light gray colonies in 5 days and dark gray in 14 days. Microscopic evaluation showed septate and branched hyphae. The conidia are ellipsoid to ovoid, with smooth walls and disarticulated, and may have one or zero septa, with an average size of 7.2–12x3-7.8 μm. Molecular analysis, using the ITS and TUB2 sequences, showed 100% identity with the phytopathogenic fungus Neoscytalidium dimidiatum, according to BLAST analysis. In the phylogenetic tree, the isolate was close to isolates from China and Vietnam. Furthermore, the pathogenicity test confirmed the fungus Neoscytalidium dimidiatum as a causal phytopathogen associated with stem canker in pitaya (Hylocereus costaricensis) in Pará, Brazil. These findings may assist in future studies to manage the disease.
Display omitted
•Neoscytalidium dimidiatum causes stem canker in pitaya (Hylocereus costaricensis) in Pará, Brazil.•Its and tub2 sequencing confirms N. dimidiatum as the causal agent of canker in pitaya.•The phylogenetic tree indicated proximity between the isolate from Pará and isolates from China and Vietnam.•Koch's postulates confirmed the pathogenicity of N. dimidiatum in pitaya cladodes.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Prihartini M, Rosmalawati S, Sriherwanto C, Mahsunah AH. 2023. Isolation and identification of osmophilic yeasts from Indonesian honeys collected from South Kalimantan, Banten, and East Java ...Provinces (Indonesia). Biodiversitas 24: 4126-4135. Certain yeasts possess the remarkable ability to thrive in the challenging conditions characterized by high sugar levels and low water activity commonly found in honey. However, limited research has been conducted on yeasts isolated from honey in Indonesia. To address this gap, honey samples were collected from three provinces in Indonesia: South Kalimantan, Banten, and East Java. A total of ten isolates were obtained and subjected to comprehensive morphological, physiological, and biochemical characterization. Among these isolates, four (M 1.4, M 1.6, M 1.8, and M 2.4) were selected for molecular identification using ITS1 and ITS4 primers. Through sequencing of the Internal Transcribed Spacer (ITS) region and performing homology analysis using BLAST on NCBI, it was determined that the M 1.4 and M 1.8 isolates belong to the Zygosaccharomyces genus with a percentage identity of ?97%. The M 2.4 isolate was classified as the Schizosaccharomyces genus with a percentage identity of ?98%, while the M 1.6 isolate was identified as Zygosaccharomyces siamensis with a percentage identity of ?99%. All four isolates exhibited osmophilic characteristics and demonstrate significant potential as valuable microorganisms for future investigations.
has emerged as one of the most important
species. It has been widely considered the second most virulent
species, only preceded by
. Besides, this species has been recognized as a very strong biofilm ...producer, surpassing
in most of the studies. In addition, it produces a wide range of other virulence factors, including: adhesion to buccal epithelial and endothelial cells; the secretion of lytic enzymes, such as proteinases, phospholipases, and hemolysins, bud-to-hyphae transition (also called morphogenesis) and the phenomenon called phenotypic switching. This is a species very closely related to
and has been easily identified with both phenotypic and molecular methods. In addition, no cryptic sibling species were yet described in the literature, what is contradictory to some other medically important
species.
is a clinically relevant species and may be the second or third etiological agent of candidemia, specifically in Latin American countries and Asia. Antifungal resistance to the azoles, polyenes, and echinocandins has already been described. Apart from all these characteristics,
has been considered an osmotolerant microorganism and this ability to survive to high salt concentration may be important for fungal persistence in saline environments. This physiological characteristic makes this species suitable for use in biotechnology processes. Here we describe an update of
, focusing on all these previously mentioned subjects.
•blaOXA-51-like is a molecular detection marker for Acinetobacter baumannii.•blaOXA-51-like was not amplified in 14 A. baumannii outbreak isolates.•ISAba19 disrupted blaOXA-51-like and the amplified ...PCR band was 1.7 kb instead of 353 bp.•Besides blaOXA-51-like, gyrB or rpoB multiplex PCR may be reliable for rapid detection of A. baumannii.
Early detection of Acinetobacter baumannii, an emerging pathogen in hospital settings, is of interest. The blaOXA-51-like family had been used as a marker to detect A. baumannii. During an infection outbreak, 14 isolates produced a 1.7-kb PCR band instead of 353 bp for this marker. This work sought the reasons behind the increased marker size. Characterisation of isolates was done by API-20NE, gyrB multiplex PCR and 16S–23S rRNA ITS sequencing. The 1.7-kb band generated and the complete blaOXA-51-like variant were sequenced to find the probable integrated element. Susceptibility testing to various antimicrobials was performed by microdilution. blaOXA-like, metallo-β-lactamases (MBLs), the ISAba1 element and the presence of ISAba1 adjacent to blaOXA-like were sought. rep-PCR, global clonal (GC) lineage determination and multilocus sequence typing (MLST) were performed to analyse the relationship among isolates. Isolates were characterised as Acinetobacter baumannii–calcoaceticus complex by API-20NE. gyrB multiplex PCR and 16S–23S ITS sequencing verified the isolates as A. baumannii. Sequencing of the 1.7-kb band revealed ISAba19 as the disrupting element. The blaOXA-51 variant was blaOXA-66, which was elongated to 2.2 kb due to ISAba19. The blaOXA-23-like family was found in 67% of isolates. MBL genes were not detected; however, ISAba1–blaOXA-23-like was characterised in carbapenem-resistant isolates (53%; 8/15). Isolates were divided into three clusters by rep-PCR. All strains were ST2 and all but one belonged to GC II. Identification of A. baumannii based only on blaOXA-51-like is not reliable. Besides blaOXA-51-like, multiplex PCR of gyrB and rpoB could provide rapid and cost-effective results.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Bemisia tabaci is an agricultural pest that interferes plant growth, as well as being an insect vector of various types of viruses, one of which is the geminivirus group. B. tabaci is called Cryptic ...Species Complex due to their similar morphology but has different genetic profiles. Climate change and increased global trading of agricultural products could increase B. tabaci population and lead to the emergence of genetic disparity. This study aims to obtain the latest information on the population homogeneity of B. tabaci in four districts of the Yogyakarta and potential differences on their nucleotide arrangements. Molecular identification was performed using PCR and primers C1-J-2198/L2-N-3914 on B. tabaci. B. tabaci COI gene sequences were then compared using a phylogenetic analysis and similarities of nucleotide bases were determined. Results showed that the populations of B. tabaci in Yogyakarta were still homogeneous showed by biotypes similarity, namely the non-B biotype. B. tabaci from Yogyakarta have nucleotide base similarity of 100% with B. tabaci from Singapore (AY686095) and Thailand (AY686092) and 99.56% with species from Bangladesh (AJ748388). Results also showed no differences in the composition of both nucleotide bases and amino acids from the four districts of B. tabaci sampling location. The homogeneous population of B. tabaci and the high incidence of yellowing disease caused by Begomovirus in chili pepper plants in the Special Region of Yogyakarta prove that it is necessary to review the current methods of controlling B. tabaci pests.
PDF
