A study was conducted to evaluate bacterial contamination in hairdressing and beauty salons in Thi-Qar at Alnasiriyah city. Samples were collected from ten different salons. The samples were ...collected from scissors,comb, razor, dryer, sink, and table tools. The isolates obtained were examined and identified using microscopic examination, colonial morphology and biochemical characteristics. Six bacterial species were isolated and identified. The bacterial isolates include Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus spp, Micrococcus Spp,Enterococusspp and Enterobacter. Isolated from some men's salons and shaving tools and the highest frequency of bacteria was in the salon of Nawras by 15%, Salon Ahmed by 12.5% , more bacterial isolation of shaving tools,sink,razor and the presence of this potential pathogen is an indication that hairdressing salons could be contributing to the spread of infection.
Pontang District located in Banten Bay has the potential for the development of shrimp farming. One of the challenges of shrimp farming is a disease caused by Vibrio bacterial. One way to control ...disease is to apply the principle of biosecurity to support sustainable aquaculture. In some cases, the Vibrio bacterial group is known to have caused serious illness in shrimp ponds. This paper reviews some of the literature on the concept of biosecurity in controlling disease for sustainable aquaculture that might be implemented in Pontang District. The role of local authorities is very important in the implementation of biosecurity. Disease control by farmers can be done by doing water management, good production processes and routine health monitoring.
Ethanol and water extracts were prepared from defatted cranberry pomace by pressurized liquid extraction and tested in bacterial cultures of L. monocytogenes, B. thermospacta, P. putida, lactic acid ...bacteria (LAB), aerobic mesophilic bacteria (AMB), and pork meat products. Anthocynanins (glucosides, galactosides and arabinosides of cyanidin and peonidins), phenolic compounds and organic acids (quinic, chlorogenic, malic and citric acids; procyanidin B3, myricetin and quercetin derivatives) were determined in the extracts. The extracts effectively inhibited the growth of tested bacteria at higher than 3.3% concentration. The effect of 2% ethanol extract additive on the inhibition of the same bacteria was also determined in non-inoculated and inoculated with bacteria pork slurry, pork burgers, and cooked ham. The results showed a significant growth inhibition of pathogenic L. monocytogenes and some other species in pork slurry, burgers and cooked ham with cranberry pomace ethanol extract as compared with the control samples. The extract also effectively inhibited the formation of oxidation indicator malondialdehyde in meat products. Slight impact of extract on some physico-chemical properties of meat products such as pH, metmyoglobin content was also observed, while it did not have significant influence on water activity. Extract addition imparted some color changes; however, it did not have negative effect on the overall sensory quality of burgers and cooked ham. High effectiveness of extract additive against pathogenic L. monocytogenes and some other tested bacteria in pork slurry, burgers and cooked ham during refrigerated storage for 16, 16 and 40 days, respectively, suggest that ethanol extract of defatted cranberry pomace may be a promising natural ingredient of meat products for increasing their microbiological safety and improving oxidative stability.
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•Cranberry pomace extracts effectivelly inhibited pathogenic/spoilage bacteria.•Ethanol extract improved oxidative stability of pork slurry, burgers and ham.•Ethanol extracts of cranberry pomace may increase shelf-life of meat products.•Ethanol had insignificant effect on other quality characteristics, except color.•Pomace extract did not have negative effects on sensory quality of products.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
The object of this study was to elucidate the bactericidal mechanism of a 222 nm Krypton Chlorine (KrCl) excilamp compared with that of a 254 nm Low Pressure mercury (LP Hg) lamp. The KrCl excilamp ...had higher bactericidal capacity against Gram-positive pathogenic bacteria (Staphylococcus aureus and L. monocytogenes) and Gram-negative pathogenic bacteria (S. Typhimurium and E. coli O157:H7) than did the LP Hg lamp when cell suspensions in PBS were irradiated with each type of UV lamp. It was found out that the KrCl excilamp induced cell membrane damage as a form of depolarization. From the study of respiratory chain dehydrogenase activity and the lipid peroxidation assay, it was revealed that cell membrane damage was attributed to inactivation of enzymes related to generation of membrane potential and occurrence of lipid peroxidation. Direct absorption of UV radiation which led to photoreaction through formation of an excited state was one of the causes inducing cell damage. Additionally, generation of ROS and thus occurrence of secondary damage can be another cause. The LP Hg lamp only induced damage to DNA but not to other components such as lipids or proteins. This difference was derived from differences of UV radiation absorption by cellular materials.
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•KrCl excilamp had more bactericidal effect than LP Hg lamp on cell suspensions in PBS.•KrCl excialmp treatment induced loss of cell membrane potential by affecting proteins and lipids.•KrCl excilamp treatment led to DNA damage by generating ROS or direct absorption.•LP Hg lamp treatment solely affected DNA integrity by direct UV absorption.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
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•PAgT composite exhibited effective disinfection on various pathogenic bacteria.•Antimicrobial behaviors between Gram +ve and Gram −ve were comparatively studied.•Silicone ...glue-immobilized PAgT system showed excellent stability and reusability.•Photocatalysis and mechanical stress showed synergistic effect on disinfection process.•The newly immobilized catalytic system holds great potential for real water sterilization.
In this study, we reported an effective visible light-assisted photocatalytic disinfection toward various representative pathogenic bacteria over a highly reusable glue-immobilized P/Ag/Ag2O/Ag3PO4/TiO2 (PAgT) system. The results showed that the PAgT photocatalyst could rapidly kill all pathogenic bacteria isolated from real water environment, including Gram-positive (+ve) (Enterococcus sp. and Staphylococcus aureus) and Gram-negative (−ve) (Escherichia coli and Salmonella), both in single and mixed bacteria system. Compared to Gram −ve bacterium, the inactivation of Gram +ve bacterium was more resistant to the photocatalytic treatment, which was mainly ascribed to their thicker peptidoglycan layers in cell walls. In addition, using silicone glue as a binder provided a strong adhesion between the solid matrix and catalyst particles thus making the immobilized PAgT highly reusable. More strikingly, it was found that the enhanced antibacterial performance of immobilized PAgT system was due to a synergistic effect of disinfection mechanism combining the improved photocatalysis on the introduction of heterojunction structure, and the mechanical stress driven from the composite sharp edge morphology. Hence, the present results of the photocatalytic disinfection toward a broad range of pathogenic bacteria, with excellent activity, stability and reusability, reveals the possibility of the developed silicone glue-immobilized PAgT in real water sterilization.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
We report a case of odontogenic brain abscess caused by Fusobacterium nucleatum. An 82-year-old man presented to us with a history of convulsions. He did not have a history of fever or headache. ...Brain magnetic resonance imaging findings suggested a brain abscess in the left frontal lobe, and the patient was initiated on treatment with anticonvulsants and antibiotics. While the lesion was monitored to assess the effect of the antibiotics, it was found to enlarge rapidly. Therefore, we performed trephine drainage, and F. nucleatum was identified on culture of the abscess fluid. Intraoral examination revealed periodontitis in the left mandibular molars. Postoperatively, his convulsions stopped with antiepileptic treatment. The patient was diagnosed as having an odontogenic abscess, and treated with antibacterial agents and oral care. This was a case of an odontogenic abscess caused by F. nucleatum in an elderly male patient. A good outcome was obtained by performing an early surgical procedure with administration of the appropriate antibacterial agent. Although it has been suggested that brain abscesses caused by Fusobacterium species may be complicated systemic and invasive infection, appropriate treatment should be administered, including timely surgical intervention and use of the appropriate antibacterial agents, because of the high sensitivity of the infection to antibiotics.
Cu-MOF nanoparticles with an average diameter of 550 nm were synthesized from 2-aminoterephthalic acid and Cu(NO3)2 by a mixed solvothermal method. The Cu-MOF nanoparticles can show peroxidase-like ...activity that can catalyze 3,3′,5,5′-tetramethylbenzidine to produce a yellow chromogenic reaction in the presence of H2O2. The presence of abundant amine groups on the surfaces of Cu-MOF nanoparticles enables facile modification of Staphylococcus aureus (S. aureus) aptamer on Cu-MOF nanoparticles. By combining Cu-MOF-catalyzed chromogenic reaction with aptamer recognition and magnetic separation, a simple, sensitive, and selective colorimetric method for the detection of S. aureus was developed.
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IJS, KILJ, NUK, PNG, UL, UM
•Recombinase polymerase amplification with CRISPR-Cas12a for nucleic acid detection were used.•As low as 10 copies of target DNA can be detected.•The RPA-Cas12a-FS method can be used in food safety ...field.
Food analysis to ensure food safety and quality are relevant to all countries. This study aimed to develop a detection technique by combining recombinase polymerase amplification with CRISPR-Cas12a for food safety (termed RPA-Cas12a-FS). Our data showed that this novel method could be detected via fluorescence intensity for the molecular identification of foodborne pathogenic bacteria, genetically modified crops, and meat adulteration. After optimization, the sensitivity and stability of RPA-Cas12a-FS was further enhanced. The RPA-Cas12a-FS system could specifically detect target gene levels as low as 10 copies in 45 min at 37 °C. The RPA-Cas12a-FS system was sensitive both using standard samples in the lab and using samples from the field, which indicated that this detection method was practical. In conclusion, a simple, rapid, and highly sensitive detection method based on CRISPR-Cas12a was developed for molecular identification in the food safety field without requiring technical expertise or ancillary equipment.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Outbreaks of foodborne diseases demand simple, rapid techniques for detecting pathogenic bacteria beyond the standard methods that are not applicable to routine analysis in the food industry and in ...the points of food consumption. In this work, we developed a sensitive, rapid and low-cost assay for detecting Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Salmonella typhimurium (S. typhi) in potable water and apple juice. The assay is based on electrical impedance spectroscopy measurements with screen-printed interdigitated electrodes coupled with magnetite nanoparticles functionalized with the antimicrobial peptide melittin (MLT). The data were analyzed with the information visualization methods Sammon's Mapping and Interactive Document Map to distinguish samples at two levels of contamination from food suitable for consumption. With this approach it has been possible to detect E. coli concentration down to 1 CFU mL−1 in potable water and 3.5 CFU mL−1 in apple juice without sample preparation, within only 25 min. This approach may serve as a low-cost, quick screening procedure to detect bacteria-related food poisoning, especially if the impedance data of several sensing units are combined.
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•Low cost detection of bacteria with melittin immobilized on magnetic nanoparticles.•Fast detection of E. coli in apple juice and potable water without sample preparation.•Sensitive analysis of E. coli with limit of detection of 1 CFU mL−1 in potable water −1.•Accurate distinction of apple juice and potable water contaminated with bacteria.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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