Grifola frondosa polysaccharide-protein complex (G. frondosa PPC) is a polymer which consists of polysaccharides and proteins/peptides linked by covalent bonds. In our previous ex vivo research, it ...has been demonstrated that a cold-water extracted G. frondosa PPC has stronger antitumor activity than a G. frondosa PPC extracted from boiling water. The main purpose of the current study was to further evaluate the anti-hepatocellular carcinoma and gut microbiota regulation effects of two PPCs isolated from G. frondosa at 4 °C (GFG-4) and 100 °C (GFG-100) in vivo. The results exhibited that GFG-4 remarkably upregulated the expression of related proteins in TLR4-NF-κB and apoptosis pathway, thereby inhibiting the development of H22 tumors. Additionally, GFG-4 increased the abundance of norank_f__Muribaculaceae and Bacillus and reduced the abundance of Lactobacillus. Short chain fatty acids (SCFAs) analysis suggested that GFG-4 promoted SCFAs production, particularly butyric acid. Conclusively, the present experiments revealed GFG-4 has the potential of anti-hepatocellular carcinoma growth via activating TLR4-NF-κB pathway and regulating gut microbiota. Therefore, G. frondosa PPCs could be considered as safe and effective natural ingredient for treatment of hepatocellular carcinoma. The present study also provides a theoretical foundation for the regulation of gut microbiota by G. frondosa PPCs.
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•GFG-4 inhibited tumor growth in H22 tumor-bearing mice.•GFG-4 enhanced levels of TNF-α, IFN-γ, IL-1β and IL-2 to activate the immune system.•GFG-4 activated TLR4-NF-κB and apoptosis signaling pathway to exert antitumor effect.•GFG-4 could regulate the composition and abundance of gut microbiota.•GFG-4 promoted short chain fatty acids production, especially butyric acid.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
A single emulsifier material is seldom sufficient to cater to the requirements of complex emulsion-based food formulations that have to function over a wide range of pH, ionic strength, storage time, ...and temperature. Food emulsions have to be designed to satisfy several requirements for use which could be achieved by combining the beneficial properties of multiple emulsifiers. The present article reviews novel biological materials that are used to design oil-in-water (o/w) emulsions. More specifically, the major focus is to discuss (o/w) interfaces stabilized by multiple biopolymers. Prevalent ways by which two or more emulsifiers could be combined is by (i) forming multi-layered emulsions and (ii) conjugation of two compounds of beneficial traits. Multilayer emulsions make use of a combination of protein, phospholipids, and carbohydrates to stabilize (o/w) interfaces. On the other hand, covalent bonding between a protein and polysaccharide is induced to form a single entity known as conjugate that is superior to the individual biopolymers in terms of emulsion stability. Therefore, properties required to maintain emulsion stability such as surface activity, solubility, steric and electrostatic repulsion, and antioxidant effects from two different hydrocolloids could be integrated.
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BFBNIB, GIS, IJS, KISLJ, NUK, PNG, UL, UM, UPUK
Protein–polysaccharide interactions are of great importance in the design of dairy formulations, as they play a key role in the formation of structure and texture in dairy products. With a detailed ...understanding of the factors affecting the interactions, the ability of charged polysaccharides to associate with the milk proteins is continuously exploited to create functional complexes, novel ingredients and delivery systems. In addition, formulations containing non-interacting polysaccharides also need to be carefully controlled, as these biopolymers may give rise to segregative phase separation, with important consequences to the stability and quality of the final matrix. As casein micelles play a major role in imparting structure to dairy products, emphasis in this review will be given to the molecular details of the interactions between polysaccharides with these protein particles. Some of the most researched polysaccharides will be highlighted in this context, and the progress made in understanding their role during structure formation of dairy matrices will be discussed. The opportunity of creating novel microstructures provided by association or/and incompatibility of milk proteins and different polysaccharides will be assessed.
Display omitted Protein–polysaccharide interactions are of great importance in the design of dairy formulations, as they play a key role in the formation of structure and texture in dairy products. In this review, we will highlight the molecular details of the interactions between some of the polysaccharides commonly employed in dairy systems and casein micelles, with focus on the progress made in understanding their role during structure formation of dairy products.
► We review protein-polysaccharide interactions in dairy formulations. ► The interactions between casein micelles and polysaccharides are highlighted. ► The role of the interactions in imparting novel microstructures is described.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Cuproptosis affects osteosarcoma locally, and the exploitation of cuproptosis-related biomaterials for osteosarcoma treatment is still in its infancy. We designed and synthesized a novel injectable ...gel of Cu ion–coordinated Tremella fuciformis polysaccharide (TFP–Cu) for antiosteosarcoma therapy. This material has antitumor effects, the ability to stimulate immunity and promote bone formation, and a controlled Cu2+ release profile in smart response to tumor microenvironment stimulation. TFP–Cu can selectively inhibit the proliferation of K7M2 tumor cells by arresting the cell cycle and promoting cell apoptosis and cuproptosis. TFP–Cu also promoted the M1 polarization of RAW264.7 cells and regulated the immune microenvironment. These effects increased osteogenic gene and protein expression in MC3T3-E1 cells. TFP–Cu could significantly limit tumor growth in tumor-bearing mice by inducing tumor cell apoptosis and improving the activation of anti-CD8 T cell-mediated immune responses. Therefore, TFP–Cu could be a potential candidate for treating osteosarcoma and bioactive drug carrier for further cancer-related applications.
•An injectable TFP-Cu gel for anti-osteosarcoma therapy was prepared.•TFP-Cu has controlled Cu2+ release in response to tumor microenvironment stimulation.•TFP-Cu can kill osteosarcoma cells, regulate immunity and promote bone formation.•The mechanism of anti-osteosarcoma is cuproptosis and the bioactivity of TFP.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
As polysaccharide from Bletilla striata (BSP) was anticipated with mucoadhesive improvement in sodium alginate (SA) microspheres, BSP was mixed with SA to construct a composite microsphere to retain ...in the gastrointestinal tract for a long time. The morphological properties, particle size and thermodynamic properties of the microspheres in combination with comprehensive evaluations in the swelling properties, mucin adsorption, ex vivo and in vivo gastric retention were determined to characterize the mucoadhesion of SA-BSP blend microspheres. Results showed that the prepared microspheres were discrete and spherical. The addition of BSP increased flexibility and reduced rigidity of SA microsphere. Furthermore, the swelling property, mucin adsorption ability and the retention rate on the gastric mucosa of SA matrix were increased after blending with BSP. Mucoadhesion tests showed the SA-BSP microspheres stayed much longer in rats' stomach than the SA microsphere did. Above all, the SA-BSP microspheres with the enhanced mucoadhesion suggested being a potential drug carrier in developing the gastroretentive drug delivery system.
•The prepared SA-BSP blend microspheres are discrete and spherical.•SA microspheres become bigger and more flexible after blending with BSP.•BSP addition improves the mucoadhesive capability of SA microspheres.•SA-BSP blend microsphere has potential as gastroretentive drug carriers.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
•Fucoidan administered could significantly reduce the blood glucose level.•Serum insulin levels in diabetic mice were increased by fucoidan.•OGTT revealed that fucoidan treatment had some effect on ...glucose disposal.•Furthermore, fucoidan altered plasma lipid levels.•Fucoidan could be a potential oral hypoglycemic agents or functional food.
In the present study, a sulfated polysaccharide fucoidan extracted from Saccharina japonica was administered to normal and alloxan-diabetic rats/mice, and its effects on glycemia, insulin and serum lipid levels were evaluated. Fucoidan administered at 200 or 1200mg/kg body weight/day could significantly reduce the blood glucose level by 22% and 34%, respectively, in alloxan-induced diabetic rats. Serum insulin levels in diabetic mice were increased by the administration of fucoidan (P<0.05). The results of an oral glucose tolerance test (OGTT) revealed that fucoidan treatment had some effect on glucose disposal after 15 days of treatment. Furthermore, fucoidan altered plasma lipid levels by lowering cholesterol, triglyceride and plasma low-density lipoprotein concentrations, while elevating plasma high-density lipoprotein cholesterol at 100 or 300mg/kg body weight/day. The results suggested that fucoidan exhibited a considerable hypoglycemic effect, possibly by stimulating pancreatic release of insulin and/or by reducing insulin metabolism. Our results indicated that fucoidan could be developed as a potential oral hypoglycemic agents or functional food for the management of diabetes.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
► Acrylamide was grafted onto xanthan gum chains. ► The effect of the thermal or the chemical activation on the grafting was studied. ► The type of activator influenced in the speed of reaching the ...equilibrium reaction. ► The increasing in the persulfate concentration reduced the grafting percentage. ► The copolymers obtained presented intermediary behavior to the parent polymers.
The present study aimed to study the reaction conditions of grafting of acrylamide on xanthan gum. It was analyzed the influence of reaction conditions, mainly type of initiator activation, initiator concentration and initiator/acrylamide ratio, on graft parameters and copolymer properties. Potassium persulfate was employed as an initiator and heating or N,N,N′,N′-tetramethylethylenediamine was used to activate the initiator. Reaction time and initiator concentration were varied and final values for grafting percentage and grafting efficiency were the same for both methods, whereas speed in reaching these values differs from one technique to another. We found that reaction time was inversely proportional to intrinsic viscosity, likely due to main chain degradation promoted by potassium persulfate (KPS); furthermore, the increasing in the KPS concentration lowers grafting percentage, acrylamide conversion and chain degradation, possibly as a result of O2 formation at high KPS concentrations.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Aging is a biological process of progressive deterioration of physiological functions, which poses a serious threat to individual health and a heavy burden on public health systems. As population ...aging continues, research into anti-aging drugs that prolong life and improve health is of particular importance. In this study, the polysaccharide from stems and leaves of
was obtained with water extraction and alcohol precipitation, and then separated and purified with DEAE anion exchange chromatography and gel filtration to obtain CVP-AP-I. We gavaged natural aging mice with CVP-AP-I and performed serum biochemical analysis, histological staining, quantitative real-time PCR (qRT-PCR) and ELISA kit assays to analyze inflammation and oxidative stress-related gene and protein expression in tissues, and 16SrRNA to analyze intestinal flora. We found that CVP-AP-I significantly improved oxidative stress and inflammatory responses of the intestine and liver, restored the intestinal immune barrier, and balanced the dysbiosis of intestinal flora. In addition, we revealed the potential mechanism behind CVP-AP-I to improve intestinal and liver function by regulating intestinal flora balance and repairing the intestinal immune barrier to regulate the intestinal-liver axis. Our results indicated that
polysaccharides possessed favorable antioxidant, anti-inflammatory and potentially anti-aging effects
.
With the continuous deepening of international research in the field of biology, more and more studies have found that polysaccharides have multiple biological functions, so that polysaccharides have ...gradually become the research objects of more and more scientists in the world, and a large number of relevant researchers have carried out Glycobiology research, most of the current research is on the separation, extraction, structural characterization and activity experiments of polysaccharides. However, at this stage, research on the structure-activity relationship of various polysaccharides extracted from plants is relatively rare, and the representation method of polysaccharide structures is not perfect, not unified, complicated in drawing, and not beautiful and convenient to read. The SNFG (Symbol Nomenclature For Glycans) method, which is the symbolic nomenclature of polysaccharides and the 3D-SNFG method, can solve the above problems well, and can use unified rules to describe and describe the molecular structure of polysaccharides, and the painting process is more convenient and more convenient. It is beautiful and makes it easier for readers to read. In this paper, the fern hemp polysaccharide molecule is taken as an example. After drawing it with chemoffice, SNFG and 3D-SNFG are used to describe it, and then compared. It is clear at a glance that the use of SNFG and 3D-SNFG methods has been widely recognized and accepted internationally, which can provide great convenience for sugar-related research and information exchange.
•Characterization of polysaccharide structure by SNFG method.•Using the 3D-SNFG method to obtain a 3D ball and stick model of the polysaccharide molecule.•The SNFG method is beautiful and convenient.•Seeing the spatial structure and monosaccharide composition of polysaccharide molecules clearly.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
This review article is dedicated to special polysaccharide esters – the polysaccharide toluenesulfonic acid esters (tosylates) and polysaccharide carbonate esters. After describing the specifics of ...the synthesis, particular emphasis is placed on the use of polysaccharide tosylates and polysaccharide phenyl carbonates for subsequent modification by nucleophilic substitution (SN) reactions. For this purpose, the advantages and limitations of the respective derivatives are discussed with regard to their application in chemical modification with nucleophiles containing functional groups. A few functional polysaccharide derivatives and their properties are presented. Finally, reactive derivatives for click chemistry approaches are featured. These can be prepared starting from the reactive intermediate of either polysaccharide tosylate or polysaccharide phenyl carbonate.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
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