The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated protein 9 (Cas9) genome‐editing system has shown great potential in biomedical applications. Although physical ...approaches, viruses, and some nonviral vectors have been employed for CRISPR/Cas9 delivery and induce some promising genome‐editing efficacy, precise genome editing remains challenging and has not been reported yet. Herein, second near‐infrared window (NIR‐II) imaging‐guided NIR‐light‐triggered remote control of the CRISPR/Cas9 genome‐editing strategy is reported based on a rationally designed semiconducting polymer brush (SPPF). SPPF can not only be a vector to deliver CRISPR/Cas9 cassettes but also controls the endolysosomal escape and payloads release through photothermal conversion under laser irradiation. Upon laser exposure, the nanocomplex of SPPF and CRISPR/Cas9 cassettes induces effective site‐specific precise genome editing both in vitro and in vivo with minimal toxicity. Besides, NIR‐II imaging based on SPPF can also be applied to monitor the in vivo distribution of the genome‐editing system and guide laser irradiation in real time. Thus, this study offers a typical paradigm for NIR‐II imaging‐guided NIR‐light‐triggered remote control of the CRISPR/Cas9 system for precise genome editing. This strategy may open an avenue for CRISPR/Cas9 genome‐editing‐based precise gene therapy in the near future.
A semiconducting polymer‐brush‐based CRISPR/Cas9 genome‐editing delivery nanoplatform is developed that can simultaneously track the in vivo distribution of the genome‐editing system and guide laser irradiation via NIR‐II imaging in real time and remote control of the CRISPR/Cas‐9 genome‐editing process noninvasively in vivo.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Poly(N-isopropylacrylamide) (PNIPAM) is broadly applicable in many fields due to its temperature-induced phase transition property. Herein, a facile method to incorporate exfoliated Ti2C3Tx ...nanosheets in the PNIPAM network is reported. Due to compatibility, stability and photothermal properties of the incorporated Ti2C3Tx nanosheets, the obtained MXene/PNIPAM composite hydrogel shows excellent photothermal properties, expanding the pure thermal-responsive property of the PNIPAM hydrogel. Based on the smart composite hydrogel, remote light-control of the microfluidic pipeline is also demonstrated.
•An easy-to-deploy and simple-to-use framework for digital-twin-based cyber-physical production system (CPPS) is proposed.•The configuration and runtime of digital-twin-based CPPS is realized based ...on digital twin modeling, dynamic resource registration, and event-driven distributed cooperation.•A prototype is implemented and verified in our lab environment.
The rapid development new generation of information technologies facilitate the emergence of cyber-physical production system (CPPS) which could pave a way to exploring new smart manufacturing solutions. Digital twin (DT) is the technical core for establishing CPPS in the context of industry 4.0. Developing an easy-to-deploy and simple-to-use DT-based CPPS is a critical research gap. In this paper, a systemic framework is proposed to provide guidelines for rapid system configuration and easy runtime of DT-based CPPS by integrating CPS, DT modeling technologies, event-driven distributed cooperation mechanisms, and web technologies. The concept of CPS node (CPSN) for manufacturing resources is established by integrating semantic information model, 3D geometric model and function modules. Various CPSNs are orchestrated as an autonomous CPPS using dynamic resource registration and binding technologies. To achieve easy runtime of DT-based CPPS, event-driven distributed cooperation among CPSNs and web-based remote control of CPPS are proposed respectively. Finally, to verify the feasibility of the proposed framework, a prototype of DT-based CPPS is implemented, based on which an exemplary case is conducted.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
We consider a basic system to securely and remotely control many IoT devices. Specifically, we require that: (1) a system manager broadcasts information to IoT devices, e.g., wireless environment, ...only the designated devices can identify operations sent from the manager; (2) each IoT device can detect (malicious) manipulation of the broadcast information and hence prevents maliciously generated operations from being executed. In this paper, we introduce anonymous broadcast authentication (ABA) as a core cryptographic primitive of the basic remote-control system. Specifically, we formally define the syntax and security notions for ABA so that it achieves the above requirements. We then show provably-secure ABA constructions and their implementations to provide their practical performance. Our promising results show that the ABA constructions can remotely control devices over a typical wireless network within a second.
This paper presents a sensor-Less haptic adaptive assistance scheme for bilateral shared controlled in telerobotics. The paper focuses on the implementation of an adaptive assistance scheme ...previously developed using force sensors at the master's side, extending it to the case where this measurement is no longer available. The experimental setup was composed of a virtual slave robot and a real master robot (a commercially-of-the-shelf, low-cost haptic device), namely the Novint Falcon haptic device. Due to the lack of sensors to measure the contact force between the human operator and the haptic control device i.e., only the position of the master robot is measured, a data-driven Unknown Input Observer with disturbance estimation augmentation is proposed, allowing the estimation of the (external) human force under plant uncertainty and external disturbances. The proposed approach is tested via a path tracking (known) with (unknown) obstacle avoidance task, and statistical analysis regarding the scheme's effectiveness is presented.
This paper presents a sensor-Less haptic adaptive assistance scheme for bilateral shared controlled in telerobotics. The paper focuses on the implementation of an adaptive assistance scheme ...previously developed using force sensors at the master's side, extending it to the case where this measurement is no longer available. The experimental setup was composed of a virtual slave robot and a real master robot (a commercially-of-the-shelf, low-cost haptic device), namely the Novint Falcon haptic device. Due to the lack of sensors to measure the contact force between the human operator and the haptic control device i.e., only the position of the master robot is measured, a data-driven Unknown Input Observer with disturbance estimation augmentation is proposed, allowing the estimation of the (external) human force under plant uncertainty and external disturbances. The proposed approach is tested via a path tracking (known) with (unknown) obstacle avoidance task, and statistical analysis regarding the scheme's effectiveness is presented. KEYWORDS: Drift-diffusion; decision-making models; adaptive control; teleoperation; haptic bilateral control Este artÃculo presenta un esquema de asistencia háptica sin sensor para telerobótica controlada compartida bilateral, más especÃficamente se enfocará en la implementación de un esquema de asistencia adaptativa desarrollado previamente por uno de los autores. Para la aplicación del esquema de asistencia adaptativa, se establece una configuración de teleoperación con un robot esclavo virtual y un robot maestro real (dispositivo de control háptico) utilizando un dispositivo háptico comercial (de bajo costo), a saber, el háptico Novint Falcon dispositivo. Un problema en la configuración de la teleoperación es la falta de sensores para medir la fuerza de contacto entre el operador humano y el dispositivo de control háptico (es decir, solo se mide la posición del robot maestro), que es necesaria para la aplicación de la asistencia adaptativa. Se propone un aumento de estimación de perturbación de un observador de entrada desconocido con una red neuronal, que permite la estimación de la fuerza humana (externa) bajo incertidumbre de la planta y perturbaciones externas. El documento presenta los resultados de implementación de un problema de seguimiento de ruta (conocido) con evitación de obstáculos (desconocidos), y se presenta un análisis estadÃstico sobre la efectividad de los esquemas. Modelos de difusión; modelos de toma de decisiones; control adaptativo; teleoperación; control bilateral háptico
We present a protocol for controlled cyclic remote preparation of an arbitrary single-qudit state via a seven-qudit cluster state. In the protocol, Alice can help the remote agent Bob prepare an ...arbitrary single-qudit state, Bob can help the agent Charlie prepare an arbitrary single-qudit state and at the same time Charlie can help Alice prepare an arbitrary single-qudit state under the controller David’s control. Alice, Bob and Charlie first perform positive operator-valued measurement (POVM) on their entangled particles according to the information of the prepared state, then perform generalized X-basis measurement. The controller performs generalized X-basis measurement on his entangled particle. The arbitrary single-qudit states can be cyclic remote prepared under the controller’s control. The protocol is more convenient in application since it only requires single-particle measurement and single-particle unitary operations for controlled cyclic remote preparation of the single-qudit states.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, SIK, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
The basic principle of quantum mechanics1 guarantees the unconditional security of quantum key distribution (QKD)2–6 at the cost of forbidding the amplification of a quantum state. As a result, and ...despite remarkable progress in worldwide metropolitan QKD networks7,8 over the past decades, a long-haul fibre QKD network without a trusted relay has not yet been achieved. Here, through the sending-or-not-sending protocol9, we achieve twin-field QKD10 and distribute secure keys without any trusted repeater over a 511 km long-haul fibre trunk that links two distant metropolitan areas. The fibre trunk contains 12 fibres in the cable, three of which are used for the quantum channel, optical synchronization and frequency locking, respectively. The remaining nine are used for classical fibre communication. Our secure key rate is around three orders of magnitude greater than that expected if the previous QKD field-test system was applied over the same length. Efficient quantum-state transmission and stable single-photon interference over such a long-haul deployed fibre pave the way to large-scale fibre quantum networks.A field test of twin-field quantum key distribution was implemented through a 511 km optical fibre. To this end, precise wavelength control of remote independent laser sources and fast time- and phase-compensation systems are developed.
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GEOZS, IJS, IMTLJ, IZUM, KILJ, KISLJ, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBMB, UL, UM, UPUK, ZAGLJ
Nanozymes are nanomaterial-based artificial enzymes. By effectively mimicking catalytic sites of natural enzymes or harboring multivalent elements for reactions, nanozyme systems have successfully ...served as direct surrogates of traditional enzymes for catalysis. With the rapid development and ever-deepening understanding of nanotechnology, nanozymes offer higher catalytic stability, ease of modification and lower manufacturing cost than protein enzymes. Additionally, nanozymes possess inherent nanomaterial properties, providing not only a simple substitute of enzymes but also a multimodal platform interfacing complex biologic environments. Recent extensive research has focused on designing various nanozyme systems that are responsive to one or multiple substrates by tailored means. Catalytic activities of nanozymes can be regulated by pH, H
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and glutathione concentrations and levels of oxygenation in different microenvironments. Moreover, nanozymes can be remotely-controlled
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different stimuli, including a magnetic field, light, ultrasound, and heat. Collectively, these factors can be adjusted to maximize the diagnostic and therapeutic efficacies of different diseases in biomedical settings. Therefore, by integrating the catalytic property and inherent nanomaterial nature of nanozyme systems, we anticipate that stimuli-responsive nanozymes will open up new horizons for diagnosis, treatment, and theranostics.
Nanozymes are nanomaterial-based artificial enzymes.
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