Wheat is an important cereal around the world and is a nutrient source in people’s diets. In this work, we focused on the potential of some biostimulators on four wheat varieties - Dropia, Glosa, ...Gruia, and Pegasus, with a focus on the effects during the first developing stages from germination to the appearance of the second leaf. Our results indicated that germination and some biochemical traits can be strongly influenced by biostimulants, with the effects also depending on the genetic background of the variety. Some treatments proved beneficial for germination, and also growth as found by the biometric measurements, while others inhibited both traits. Some of the biostimulants increased the concentration of the photosynthetic compounds, thus being recommended for use during all stages of wheat development. Dropia, Glosa, and Gruia reacted positively in most tests, their germination and plant and root development were stimulated by Super fifty, Atonik, Asfac, and Cropmax. Thus, we recommend the use of these biostimulants in the first development stages. The concentrations of photosynthetic pigments increased after treatment with Asfac, Atonik, and Cropmax. The treatment showing the most inhibitory effects was Lebosol.
Isoflavone, which are mainly found in soybeans, are a secondary metabolite with a variety of physiological functions. In recent years, increasing the isoflavone content of soybeans has received ...widespread attention. Although ethephon treatment significantly increased isoflavone content in soybean sprouts, it also had a certain inhibitory effect on the growth of sprouts. Melatonin (MT), as a new type of plant hormone, not only alleviated the damage caused by abiotic stress to plants, but also promoted the synthesis of secondary metabolites. In this study, we aimed to elucidate the mechanism of exogenous MT in regulating the growth and development, and the metabolism of isoflavone in soybean sprouts under ethephon treatment. The results indicated that MT alleviated the adverse effects of ethephon treatment on soybean sprouts by increasing the activities of superoxide dismutase, peroxidase, catalase, and the expression of their corresponding genes, as well as decreased the content of malondialdehyde and hydrogen peroxide. In addition, MT further increased the isoflavone content by up-regulating the expression level of isoflavone synthesis genes and increased the activities of phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase under ethephon treatment. This study provided technical support and reference value for the production of high-quality soybean sprouts to a certain extent.
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•Combined MT and ethephon treatment promoted the isoflavone biosynthesis in soybeans.•MT regulated isoflavone metabolism related enzyme activities and gene expression.•MT activated the antioxidant defense system under ethephon treatment.•MT regulated the activity of antioxidant enzymes and the expression of their corresponding genes.•MT up-regulates the expression level of 1-aminocyclopropane-1-carboxylic acid synthase.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Alfalfa sprouts have been linked to numerous North American outbreaks of
in recent years. Conventionally, treatments involving chlorine, heat, and irradiation are used for alfalfa seed sanitation. ...However, such treatments may be highly variable in their efficacy for pathogen control and/or detrimental to sprout quality, therefore negatively perceived by consumers advocating for natural alternatives. The usage of bacteriophages for pathogen control in sprouts has been previously explored, although with conflicting and inconsistent results. Lytic phages, viral predators of bacteria, represent an attractive approach as they provide several advantages compared to conventional treatments, such as their high specificity for bacterial targets and their ubiquity in nature. In this study, four
phages were isolated from British Columbia, Canada and characterized with respect to host range, burst size, latent period, and environmental stability to assess their potential to control
. Phage isolate SI1 showed the greatest host range, highest burst size and shortest latent period, greatest stability across all pH and temperatures and was the most effective in control of
Enteritidis
. Therefore, SI1 was chosen for treatment of sprouting alfalfa seeds artificially contaminated with
Enteritidis with a multiplicity of infection (MOI) of ∼110 PFU/CFU. A significant (
< 0.05) reduction of 38.3 ± 3.0% of viable
cells was observed following two h of phage treatment. On days two to six of the sprouting process, reductions of
were also observed, but were not significant compared to the control (
> 0.05). It was further demonstrated that the sprout yield was not significantly (
> 0.05) affected by phage treatment. These results highlight the potential of phages recovered from the British Columbia environment for use as biocontrol agents against
, although differing efficacies
was observed. Moreover, the effectiveness of SI1 to significantly (
< 0.05) control
on sprouting alfalfa seeds on day 1 of treatment was demonstrated. Although promising, future work should aim to optimize this treatment to achieve more effective, and longer lasting, biocontrol of
in sprouting alfalfa seeds.
Herein, we report the development of a matrix solid-phase dispersion (MSPD) extraction method for determining flavonoids (orientin, isoorientin, vitexin, isovitexin, quercetin-3-O-robinobioside, and ...rutin) from common buckwheat sprouts (CBS) and Tartary buckwheat sprouts (TBS) by high-performance liquid chromatography (HPLC-PDA). Key parameters, including sorbent type, sorbent-to-sample ratio, extraction solvent (type, concentration, and volume), and extraction time were optimized. The optimized MSPD extraction conditions include a C18-sorbent/sample ratio of 2:1 (w/w) total mixture weight = 100 mg and 10 min of static extraction with 5 mL of 80% ethanol. The optimized MSPD extraction method was satisfactorily validated for all target flavonoids from both CBS and TBS. The validated method was also more effective and efficient compared to ultrasound- and homogenate-assisted extraction techniques. The overall results show that the MSPD extraction method developed in this study is a simple, effective, and efficient technique for the HPLC analysis of flavonoids in CBS and TBS.
•MSPD extraction of flavonoids in buckwheat sprouts for HPLC was studied.•Parameters affecting MSPD extraction efficiency were optimized.•A simple, effective, efficient, and validated MSPD extraction method was developed.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Background: Gambier has a problem with low production levels which is caused by the use of inferior quality plants that come from inferior seeds. This study aims to determine the optimal harvesting ...age for producing gambir seeds with optimal physiological quality. The maturity phase of the fruit became the basis for determining the timescalation of the harvest that count from the day after anthesis. The research was carried out from June to December of 2021. This research was conducted at the Seed Technology Laboratory, Faculty of Agriculture andalas University, Padang. The plant material utilized is the seed of the Mancik Riau gambier. Methods: The research employed a Completely Randomized Design (CRD) with five treatments based on days after anthesis (DAA) because at this phase the plants pollinated and the fruit maturity phase begin and it used three replication : seeds harvested at 110-112 DAA, 107-109 DAA, 104-106 DAA, 101-103 DAA and 98-100 DAA. Quantitative data are analyzed at a 5% significance level using Analysis of Variance; if the effect is significant, the Duncan’s Multiple Range Test (MRT) is performed; and qualitative data are presented descriptively. Result: The results revealed that the harvest age of 98-100 DAA provided the highest viability and vigor, as measured by germinated power testing at 80.50%, seed growth potential testing at 81.83%, T50 test, index value testing at 11.04 and soil emergence testing at 84.33%, indicating that the plant had reached physiological maturity.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Sprouting induces activation and de novo synthesis of hydrolytic enzymes that make nutrients available for plant growth and development. Consumption of sprouted grains is suggested to be beneficial ...for human health. Positive consumer perceptions about sprouted cereals have resulted in new food and beverage product launches. However, because there is no generally accepted definition of “sprouting,” it is unclear when grains are to be called sprouted. Moreover, guidelines about how much sprouted grain material food products should contain to exert health benefits are currently lacking. Accordingly, there is no regulatory base to develop appropriate food labeling for “sprouted foods.” This review describes the nutritional and technological properties of sprouted grains in relation to processing conditions and provides guidelines to optimize sprouting practices in order to maximize nutritive value. Relatively long sprouting times (3 to 5 days) and/or high processing temperatures (25 to 35 °C) are needed to maximize the de novo synthesis and/or release of plant bioactive compounds. Nutrient compositional changes resulting from sprouting are often associated with health benefits. However, supportive data from clinical studies are very scarce, and at present it is impossible to draw any conclusion on health benefits of sprouted cereals. Finally, grains sprouted under the above‐mentioned conditions are generally unfit for use in traditional food processing and it is challenging to use sprouted grains as ingredients without compromising their nutrient content. The present review provides a basis for better defining what “sprouting” is, and to help further research and development efforts in this field as well as future food regulations development.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Total antioxidant capacity, total phenolic contents (TP) and anthocyanins contents (ANT) were determined in
Amaranthus cruentus and
Chenopodium quinoa seeds and sprouts. Antioxidant activity of the ...investigated seeds decreased in the following order: quinoa, amaranth v. Rawa, amaranth v. Aztek for FRAP and quinoa, amaranth v. Aztek, amaranth v. Rawa for both ABTS and DPPH. Sprouts activity depended on the length of their growth, and the peak values were reached on the fourth day in the case of amaranth and on the sixth day in the case of quinoa. The data obtained by the three methods showed significant correlation between TP content in seeds and sprouts. In sprouts grown in the daylight and in the darkness we observed some significant changes of TP, ANT and antioxidant activity. Amaranth and quinoa seeds and sprouts can be used in food, because it is a good source of ANT and TP with high antioxidant activity.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Epidemiological studies have shown that regular consumption of fruits and vegetables is associated with reduced risk of chronic diseases. It is recommended to increase consumption of fruits and ...vegetables to prevent chronic diseases related to free radical-induced oxidative stress. Different varieties of fruits and vegetables provide different vitamins, phenolics, flavonoids, minerals, and dietary fibers for optimal health benefits. Mung bean sprouts are one of the major vegetables in human diet. However, the profiles of phytochemicals and effect of germination on phytochemical content and antioxidant activity of mung bean sprouts have not been studied. The objective of this study was to determine the effect of germination on phytochemical profiles and antioxidant activity of mung bean sprouts. Germination of mung beans dramatically increased vitamin C content in mung bean sprouts in a time-dependent manner and reached the peak on day 8 of germination up to 285 mg/100 g DW, almost 24 times higher than the initial concentration in mung bean seeds (p < 0.05). On fresh weight basis, one serving of mung bean sprouts (about 104 g) provides 21.6 mg of vitamin C, which could meet 36% of Daily Value (DV). In addition, the germination dramatically increased total phenolic compounds and total flavonoids in mung bean sprouts in a time-dependent manner, up to 4.5 and 6.8 times higher than the original concentration of mung bean seeds, respectively. Quercetin-3-O-glucoside content was significantly increased in mung bean sprouts after germination. The total antioxidant activity of mung bean sprouts was increased by 6 times higher than that of mung bean seeds. Therefore, the germination of mung bean sprouts significantly increased phytochemical content, vitamin C content, and antioxidant activity.
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IJS, KILJ, NUK, PNG, UL, UM, UPUK
In order to achieve the enrichment of sulforaphane, polyphenols, flavonoids and other bioactive substances in broccoli sprouts so as to reduce production costs, this study used plasma treatment ...induction method to treat broccoli sprouts. The plasma treatment conditions and its effects on the sprouts of different varieties of broccoli were studied. The results showed that the plasma treatment voltage and time had significant effects(P<0.05) on the germination state and the contents of bioactive compounds in broccoli sprouts. Under the conditions of voltage 30 kV and treatment time of 2 min, the germination rate, bud length and bud weight of broccoli sprouts after 5 days germination were significantly increased compared with the control. In addition, the sulforaphane content reached 3.24 mg/g DW, which was 6.75 times higher than that of untreated samples. There were significant differences (P<0.05) in the sulforaphane, total polyphenols, total flavonoids and antioxidant activity among different broccoli sprouts
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