•Simultaneous intercropping strategy allowed to improve ecological services.•Relay intercropping strategy guaranteed winter wheat yield and protein content.•Organic fertilizer decreased ecological ...services and improved wheat production.•Simultaneous intercropping permitted to maintain services under high N availability.
Nitrogen (N) deficiency and weed infestation are the main factors limiting the yield and quality of organic soft winter wheat (Triticum aestivum L.). If forage legumes are associated with wheat, simultaneously or successively, they can help to reduce the impact of limiting factors through the ecological services they provide. The aim of this study was to evaluate two intercropping strategies to increase soft winter wheat yield and protein content and improve the provided ecological services (mainly N provisioning and weed control) by white clover (Trifolium repens L.).
White clover (Trifolium repens L. cv Aberdaï) was intercropped with soft winter wheat (Triticum aestivum L. cv Renan) under organic conditions. Two strategies were compared, simultaneous intercropping versus relay intercropping using three field experiments. A control treatment with sole wheat crop was included in the three field experiments. Fertilization management on organic wheat was also tested (0 versus 100 kg N applied at spring time) to evaluate its incidence on cash and cover crop yields and protein content.
White clover shoot dry matter (DM) was significantly higher in simultaneous intercropping compared to relay intercropping at wheat harvest (2.2 vs. 0.1 Mg DM ha−1) and at cover destruction (4.5 vs. 3.1 Mg DM ha−1). Wheat grain yield was not affected by the intercropping strategy while the protein content was significantly lower under simultaneous intercropping system (9.1% of the grain DM) compared to relay intercropping or in wheat as sole crop (10.0% of the grain DM). At cover destruction, both simultaneous and relay intercropped white clover significantly decreased weed shoot DM compared to sole wheat crop (−1.4 Mg DM ha−1 for relay intercropping and −1.8 Mg DM ha−1 for simultaneous intercropping). In comparison to relay intercropping strategy, N accumulation in white clover shoot DM was higher under simultaneous intercropping strategy at wheat harvest (52 vs. 2 kg N ha−1) and at cover destruction (123 vs. 83 kg N ha−1).
In conclusion, our study has highlighted the positive effect of combining a simultaneous intercropping strategy with high N availability to guarantee a sufficient level of legume shoot DM as rapidly as possible to increase both N accumulation and weed control services, thus reducing the risk of impairing winter wheat yield and protein content.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Grazed grass is the cheapest feed available for dairy cows in temperate regions; thus, to maximize profits, dairy farmers must optimize the use of this high-quality feed. Previous research has ...defined the benefits of including white clover (Trifolium repens L.) in grass swards for milk production, usually at reduced nitrogen usage and stocking rate. The aim of this study was to quantify the responses in milk production of dairy cows grazing tetraploid or diploid perennial ryegrass (Lolium perenne L.; PRG) sown with and without white clover but without reducing stocking rate or nitrogen usage. We compared 4 grazing treatments in this study: tetraploid PRG-only swards, diploid PRG-only swards, tetraploid with white clover swards, and diploid with white clover swards. Thirty cows were assigned to each treatment, and swards were rotationally grazed at a farm-level stocking rate of 2.75 cows/ha and a nitrogen fertilizer rate of 250 kg/ha annually. Sward white clover content was 23.6 and 22.6% for tetraploid with white clover swards and diploid with white clover swards, respectively. Milk production did not differ between the 2 ploidies during this 4-yr study, but cows grazing the PRG-white clover treatments had significantly greater milk yields (+596 kg/cow per year) and milk solid yields (+48 kg/cow per year) compared with cows grazing the PRG-only treatments. The PRG-white clover swards also produced 1,205 kg of DM/ha per year more herbage, which was available for conserving and buffer feeding in spring when these swards were less productive than PRG-only swards. Although white clover is generally combined with reduced nitrogen fertilizer use, this study provides evidence that including white clover in either tetraploid or diploid PRG swards, combined with high levels of nitrogen fertilizer, can effectively increase milk production per cow and per hectare.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The objective of this study was to quantify the farm gate nitrogen (N) offset potential of perennial ryegrass (Lolium perenne L.; PRG) white clover (Trifolium repens L.; WC) swards by comparing the ...herbage and milk production from dairy farmlets that were simulations of full farming systems. A study was established where 120 cows were randomly assigned to 4 farmlets of 10.9 ha (stocking rate: 2.75 cow/ha), composed of 20 paddocks each. Cows were fed 526 kg of DM of concentrate on average each year. The 4 grazing treatments were PRG-only at 150 or 250 kg of N/ha and PRG-WC at 150 or 250 kg of N/ha. Cows remained in their treatment group for an entire grazing season and were re-randomized as they calved across treatments each year. As cows calved in the spring as standard practice in Ireland, they were rotationally grazed from early February both day and night (weather permitting) to mid-November, to a target postgrazing sward height of 4.0 cm. Mean sward WC content was 18.1% and 15.4% for the 150 and 250 kg of N/ha PRG-WC treatments, respectively over the 3-yr period. When WC was included, lowering the N rate did not reduce pregrazing yield, pregrazing height, or herbage removed, but those factors decreased significantly when WC was absent. Total annual herbage DM production was 13,771, 15,242, 14,721, and 15,667 kg of DM/ha for PRG-only swards receiving 150 or 250 kg of N/ha and PRG-WC swards receiving 150 or 250 kg of N/ha, respectively. In addition, when WC was present, compressed postgrazing sward heights were lower (4.10 vs. 4.21 cm) and herbage allowance (approximately 17 kg/cow feed allocation per cow per day) higher than the high-N control (+ 0.7 kg of DM/cow per day). There was a significant increase in milk production, both per cow and per hectare, when WC was included in PRG swards. Over the 3-yr study, cows grazing PRG-WC had greater milk (+304 kg) and milk solids (+31 kg of fat + protein) yields than cows grazing PRG-only swards. This significant increase in milk production suggests that the inclusion of WC in grazing systems can be effectively used to increase milk production per cow and per hectare and help offset nitrogen use. This result shows the potential to increase farm gate N use efficiency and reduce the N surplus compared with PRG-dominant sward grazing systems receiving 250 kg of N/ha, without negatively affecting milk solids yield or herbage production, thus increasing farm profit by €478/ha.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Istraživanje je provedeno u laboratorijskim uvjetima naklijavanjem kultivara sjemena bijele djeteline (Trifolium repens L.) u klima komori uz prethodno tretiranje sjemena magnetskim poljem. ...Predtretman sjemena stacioniranim magnetskim poljem od 250 mT proveden je na dva kultivara bijele djeteline, Apolo i Merlyn. Sjeme je bilo izloženo neodimijskom magnetu različitom vremenu trajanja: kontrola (bez tretmana), 5 min., 30 min. i 60 min. Ispitivanje svojstava sjemena provedeno je u klima komori po pravilima ISTA. Određena su svojstva energija klijanja i klijavost sjemena, dužina korijena i stabljike te izračunata ukupna dužina klijanaca. Vrijeme izloženosti magnetskom polju (MP) značajno je utjecalo na dužinu korijena (p<0,01) i ukupnu dužinu klijanaca (p<0,05). Između kultivara dobivene su značajne razlike za energiju klijanja i klijavost sjemena (p<0,01), dužinu stabljike i ukupnu dužinu klijanaca (p<0,05). Kultivari su različito reagirali na vrijeme izloženosti magnetskom polju te su dobivene značajne interakcije između tretmana MP i kultivara za sva ispitivana svojstva osim za dužinu stabljike klijanaca.
The study was carried out under laboratory conditions by germinating cultivars of white clover (Trifolium repens L.) in a climate chamber with pretreatment of the seeds with a magnetic field. The pretreatment of the seeds with a stationary magnetic field of 250 mT was carried out on two white clover varieties, Apolo and Merlyn. The seeds was exposed to a neodymium magnet for different times: control (no treatment), 5 minutes, 30 minutes and 60 minutes. Seed properties was tested in a climate chamber according to ISTA rules. The properties of germination energy and seed germination, root and stem length and total seedling length were calculated. The time of exposure to the magnetic field (MF) significantly influenced root length (p<0.01) and total seedling length (p<0.05). Significant differences between cultivars were found for germination energy and seed germination (p<0.01), stem length and total seedling length (p<0.05). Cultivars responded differently to the time of magnetic field exposure and significant interactions were found between MF treatment and cultivars for all tested traits except seedling stem length.
The objective of this study was to compare the economic performance of 2 sward types perennial ryegrass (PRG; Lolium perenne L.) sown with or without white clover (Trifolium repens L.) grazed by 3 ...cow genotypes. Physical performance data were collected from a 4-yr systems experiment based at Clonakilty Agricultural College, Clonakilty, Co. Cork, Ireland. The experiment compared 2 sward types (PRG-only swards and PRG–white clover swards), with each sward type being grazed by cows from 3 genotypes Holstein-Friesian (HF), Jersey × HF (JEX), and Norwegian Red × JEX (3-way). All systems were stocked at 2.75 cows/ha with fixed fertilizer applications and concentrate supplementation. The data supplied 6 production systems (2 sward types × 3 cow genotypes). The production systems were modeled using the Moorepark Dairy Systems Model (stochastic budgetary simulation model) under 2 scenarios, one in which land area was fixed and one in which cow numbers were fixed. The analysis was completed across a range of milk prices, calf prices, and reseeding programs. The analysis showed that in the fixed-land scenario with a milk price of €0.29/L, adding white clover to PRG swards increased profitability by €305/ha. In the same fixed-land scenario, JEX cows were most profitable (€2,606/ha), followed by 3-way (€2,492/ha) and HF (€2,468/ha) cows. In the fixed-cow scenario, net profit per cow was €128 greater for PRG–white clover swards compared with PRG-only swards. In this scenario, JEX was the most profitable per cow (€877), followed by HF (€855) and 3-way (€831). The system that produced the highest net profit was JEX cows grazing PRG–white clover swards (€2,751/ha). Regardless of reseeding frequency or variations in calf value, JEX cows grazing PRG–white clover swards consistently produced the highest net profit per hectare.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
As beneficial microorganisms in the soil, arbuscular mycorrhizal fungi (AMF) and rhizobia (Rh) form a mutualistic symbiosis with plant roots, while it is unclear whether AMF and Rh synergistically ...promote root colonization, plant growth, and nitrogen (N) acquisition during the colonization. The aim of this study was carried out to analyze the effect of inoculation with an arbuscular mycorrhizal fungus (
Paraglomus occultum
) and a rhizobium (
Rhizobium trifolii
), singly and in combination, on root mycorrhizal colonization and nodules as well as changes in plant growth, hydrogen peroxide (H
2
O
2
), and N acquisition of white clover over a period of 90 days. Double inoculation of AMF and Rh favored root AMF colonization and nodules compared to single inoculation. AMF colonized roots earlier than the formation of root nodules, with AMF colonized the root nodules after 30 days. H
2
O
2
(a signaling molecule for plant defense against microbial infestation) was increased in roots from day 5 to day 10 following dual inoculation and single AMF inoculation, and from day 10 to day 15 following single Rh inoculation, indicating a pre-response of roots to AMF colonization during the two microbial colonization. At 20 days after inoculation, single or double inoculation of AMF and Rh showed positive effects on leaf and root biomass production and plant height, while improved effects on taproot length began at 15 days of inoculation, along with the improved magnitude most prominently with double inoculation. The earliest effect of significant improvement in leaf N appeared on day 10 of double inoculation, while the improvement in root N started 15 days later, following the prominent effect with double inoculation among three treatments. It is concluded that AMF and Rh could synergistically promote each other’s colonization in roots, thus further enhancing plant growth and N acquisition.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Red clover (
L.), a key perennial pastoral species used globally, can strengthen pastural mixes to withstand increasingly disruptive weather patterns from climate change. Breeding selections can be ...refined for this purpose by obtaining an in-depth understanding of key functional traits. A replicated randomized complete block glasshouse pot trial was used to observe trait responses critical to plant performance under control (15% VMC), water deficit (5% VMC) and waterlogged conditions (50% VMC) in seven red clover populations and compared against white clover. Twelve morphological and physiological traits were identified as key contributors to the different plant coping mechanisms displayed. Under water deficit, the levels of all aboveground morphological traits decreased, highlighted by a 41% decrease in total dry matter and 50% decreases in both leaf number and leaf thickness compared to the control treatment. An increase in root to shoot ratio indicated a shift to prioritizing root maintenance by sacrificing shoot growth, a trait attributed to plant water deficit tolerance. Under waterlogging, a reduction in photosynthetic activity among red clover populations reduced several morphological traits including a 30% decrease in root dry mass and total dry matter, and a 34% decrease in leaf number. The importance of root morphology for waterlogging was highlighted with low performance of red clover: there was an 83% decrease in root dry mass compared to white clover which was able to maintain root dry mass and therefore plant performance. This study highlights the importance of germplasm evaluation across water stress extremes to identify traits for future breeding programs.
The Bolesław waste heap in South Poland, with total soil Zn concentrations higher than 50,000 mg kg
, 5,000 mg Pb kg
, and 500 mg Cd kg
, is a unique habitat for metallicolous plants, such as
L. The ...purpose of this study was to characterize the association between
and its microbial symbionts, i.e.,
bv.
and mycorrhizal fungi and to evaluate its applicability for phytostabilization of metal-polluted soils. Rhizobia originating from the nutrient-poor waste heap area showed to be efficient in plant nodulation and nitrogen fixation. They demonstrated not only potential plant growth promotion traits
, but they also improved the growth of
plants to a similar extent as strains from a non-polluted reference area. Our results revealed that the adaptations of
to high Zn-Pb-Cd concentrations are related to the storage of metals predominantly in the roots (excluder strategy) due to nodule apoplast modifications (i.e., thickening and suberization of cell walls, vacuolar storage), and symbiosis with arbuscular mycorrhizal fungi of a substantial genetic diversity. As a result, the rhizobia-mycorrhizal fungi-
association appears to be a promising tool for phytostabilization of Zn-Pb-Cd-polluted soils.
Phenotypic and genetic clines frequently evolve due to varying selection along environmental gradients. However, the specific environmental factors that impose differential selection are often ...multivariate and difficult to tease apart. We addressed this complexity using a factorial manipulation of watering, soil nutrients and (simulated) herbivory in controlled conditions to better understand the agents of selection driving the evolution of clines in a polymorphic chemical antiherbivore defence, hydrogen cyanide (HCN), of the plant white clover Trifolium repens. We found the presence or absence of the two metabolic components required for HCN production (cyanogenic glucosides and linamarase) to be more prominent determinants of vegetative growth and sexual reproduction in T. repens than HCN itself. We also found that the ability to produce one or both of cyanogenic glucosides or linamarase resulted in a growth advantage under drought and simulated herbivory that outweighs the metabolic cost of their production. These results support the view that the metabolic components underlying HCN play important roles beyond defence by increasing plant tolerance to stress. The growth advantage under drought, however, was diminished in the absence of nutrient addition, consistent with multivariate interactions as drivers of selection. This study provides novel insight into how a cosmopolitan plant has adapted to environmental gradients, and more generally, highlights the importance of considering interactions between multiple environmental factors when studying the evolution of phenotypic and genetic clines.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
White clover is a widely grown temperate legume forage with high nutritional value. Research on the functional genomics of white clover requires a stable and efficient transformation system. In this ...study, we successfully induced calluses from the cotyledons and leaves of 10 different white clover varieties. The results showed that the callus formation rate in the cotyledons did not vary significantly among the varieties, but the highest callus formation rate was observed in 'Koala' leaves. Subsequently, different concentrations of antioxidants and hormones were tested on the browning rate and differentiation ability of the calluses, respectively. The results showed that the browning rate was the lowest on MS supplemented with 20 mg L
AgNO
and 25 mg L
VC, respectively, and the differentiation rate was highest on MS supplemented with 1 mg L
6-BA, 1 mg L
KT and 0.5 mg L
NAA. In addition, the transformation system for
-mediated transformation of 4-day-old leaves was optimized to some extent and obtained a positive callus rate of 8.9% using green fluorescent protein (GFP) as a marker gene. According to our data, by following this optimized protocol, the transformation efficiency could reach 2.38%. The results of this study will provide the foundation for regenerating multiple transgenic white clover from a single genetic background.
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