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Pridobivanje transglutaminaze iz bakterije Streptomyces platensis = Extraction of transglutaminase from bacterium Streptomyces platensis : magistrsko deloLang, VidaStreptomyces platensis je bakterija iz rodu Streptomyces. V rod Streptomyces spadajo nitaste bakterije, ki jih najdemo v prsti. Bakterije Streptomyces uspevajo pri pH 6,5 % 8,0 in v temperaturnem ... območju med 25 °C in 35 °C. Za S. platensis je značilna proizvodnja zunajceličnih encimov, ki se uporabljajo za industrijske namene. Namen magistrske naloge je bil ugotoviti kako sprememba pH v celični suspenziji S. platensis vpliva na maso celic S. platensis, aktivnost encima transglutaminaza in koncentracijo skupnih proteinov v bakterijski suspenziji S. platensis. Celično suspenzijo S. platensis smo inkubirali v inkubacijskem stresalniku 8 dni pri temperaturi 28 °C in ob rahlem stalnem stresanju. Naredili smo 3 izvedbe, pri katerih smo v procesu inkubiranja celične suspenzije S. platensis spreminjali pogoje pH. Pri vseh 3 izvedbah inkubiranja celične suspenzije S. platensis v odvisnosti od inkubacijskega časa smo ugotovili, da medtem ko se je pH celične suspenzije zniževal, so koncentracija proteinov, aktivnost transglutaminaze in prirast suhe mase celic naraščale. Po treh različnih izvedbah se je izkazalo, da je za gojenje celične suspenzije S. platensis najugodnejši začetni pH 6,7 in inkubiranje pri temperaturi 28 °C ob rahlem stresanju ter brez dodatnega spreminjanja pH. Spreminjanje oziroma uravnavanje pH na začetku procesa ali med celotnim procesom inkubiranja celične suspenzije S. platensis ni ugodno za uspešno gojenje bakterije S. platensis, saj se celoten proces pospeši in bakterije hitreje propadejo.Type of material - master's thesis ; adult, seriousPublication and manufacture - Maribor : [V. Lang], 2017Language - slovenianCOBISS.SI-ID - 23361288
Author
Lang, Vida
Other authors
Leitgeb, Maja |
Čolnik, Maja
Topics
magistrska dela |
S. platensis |
aktivnost transglutaminaze |
koncentracija proteinov |
pH |
masa celic |
master theses |
S. platensis |
activity of transglutaminase |
concentration of proteins |
pH |
cellular mass
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oddelek za odrasle Mn 0000000054 LANG, Vida Pridobivanje ... IN: 10272682 oddelek za odrasle Mn 54 LANG, Vida Pridobivanje ... IN: 10272682 |
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Lang, Vida | 53594 |
Leitgeb, Maja | 00534 |
Čolnik, Maja | 31616 |
Source: Personal bibliographies
and: SICRIS
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