Različni postopki čiščenja virusne ribonukleinske kisline iz iztrebkov; Different methods for RNA extraction from faecal speciments : diplomska naloga

(UL)

Različni postopki čiščenja virusne ribonukleinske kisline iz iztrebkov = Different methods for RNA extraction from faecal speciments : diplomska naloga

Hafner Maček, Katarina

Iz vzorcev iztrebkov, ki so vsebovali rotaviruse ali kaliciviruse, smo s tremipostopki izolirali virusno RNK in ugotavljali uspesnost izolacije. Uporabili smo izolacijo s silicijevim dioksidom in ... gvanidinijevim tiocianatom,izolacijo s trizolom in komercialni komplet RNAqueousTM-4PCR Kit. Pri rotavirusih smo RNK dokazali direktno z elektroforezo na poliakrilamidnem gelu ob prisotnosti natrijevega dodecilsulfata, pri kalicivirusih pa smo izvedli se verizno reakcijo s polimerazo s predhodno reverzno transkripcijo inprodukte dokazali z elektroforezo na agaroznem gelu. Pri obeh vrstah virusovse je za najboljsi naèin izolacije izkazala izolacija s trizolom.

Type of material - undergr. thesis

Publication and manufacture - Ljubljana : [Hafner M.K.], 2001

Language - slovenian

COBISS.SI-ID - 1028977

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Drinovec, Borut | Poljšak-Prijatelj, Mateja

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Library	Call number – location, accession no. ...	Copy status
Faculty of Pharmacy, Lj.	Knjižnica dp 616 Hafner M.Katarina Različni IN: 0005799	available - reading room

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Year	Impact factor		Edition		Category		Classification
Year	JCR	SNIP	JCR	SNIP	JCR	SNIP	JCR	SNIP

Links to authors' personal bibliographies	Links to information on researchers in the SICRIS system
Drinovec, Borut	03174
Poljšak-Prijatelj, Mateja	08755

Source: Personal bibliographies and: SICRIS

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