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Three-dimensional observations on thick biological speciments by high voltage electron microscopyNagata, TetsujiThick biological specimens prepared as whole mount cultured cells or thick sections from embedded tissues were stained with histochemical reactions, suchas thiamine pyrophosphatase, ... glucose-6-phosphatase, cytochrome oxidase, acid phosphatase, DAB reactions and radioautography, to observe 3-D ultrastructures of cell organelles producing stereo-pairs by high voltage electron microscopy at accerelating voltages of 400-1000 kV. The organelles demonstrated were Golgi apparatus, endoplasmic reticulurn, mitochondria, lysosomes, peroxisomes. pinocytotic vesicles and incorporations of radioactivecompounds. As the results, those cell organelles were observed 3-dimensionally and the relative relationships between these organelles were demonstrated.Source: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (Letn. 19, št. 1, March 2000, str. 51-56)Type of material - article, component partPublish date - 2000Language - englishCOBISS.SI-ID - 11478745
Author
Nagata, Tetsuji
Topics
Organelles |
Ultrastructure |
Liver |
Cytology |
Anatomy and histology |
Rats, wistar |
Histocytochemistry |
Microscopy, electron |
Golgi apparatus |
Endoplasmic reticulum |
Lysosomes |
Microbodies |
Thiamine pyrophosphatase |
Glucose-6-phosphatase |
Cytochrome-c oxidase |
Acid phosphatase
source: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (Letn. 19, št. 1, March 2000, str. 51-56)
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