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Opis lokusov rrr bakterijskih vrst Prevotella ruminicola in Prevotella bryantii : doktorska disertacija = Characterization of rrn loci from bacterial species Prevotella ruminicola and Prevotella bryantii : doctoral dissertationPeterka, MatjažKlonirali smo operon ribosomskih RNA vampnih bakterij Prevotella bryantii B|4 in Prevotella ruminicola 23. Operon rRNA P. ruminicola 23, rrn23, je sestavljen iz genov za 16S, 23S in 5S rRNA, ki so od ... 5' konca proti 3' koncu povezani v omenjenem vrstnem redu. Med genoma za 16S in 23S rRNA sta gena za tRNA [zgoraj] Ile in tRNA [zgoraj] Ala. V kontrolni regiji operona, pred 5' koncem gena za 16S rRNA je promoter P1, ki ima značilnosti promoterjev sorodnih vrst iz rodu Bacteroides. Pred promoterjem je regija, ki ima značilnosti elementa UP, med promotorjem in 5' koncem gena za 16S rRNA pa je regija, ki verjetno vsebuje elemente antiterminacijskega mehanizma. Takoj za 3' koncem gena za 5S rRNA je od faktorja rho neodvisen terminator. Podobno strukturo kot rrn23 ima tudi operon rRNA P. bryantii B|4, rrnB|4. V operonu so geni za 16S, 23S, 5S rRNA in tRNA [zgoraj] Ile ter tRNA [zgoraj] Ala, ki so organizirani enako kot v rrn23. V kontrolni regiji operona je prav tako en promotor, katerega regije -10 in -35 so identične kot v rrn23. Pred 5' koncem promotorja je regija, ki ustreza sekvenci elementa UP. Med promotorjem in 5' koncem gena za 16S rRNA je regija, ki verjetno vsebuje elemente antiterminacijskega mehanizma. Takoj za 3' koncem gena za 5SrRNA je terminatorneodvisen od faktorja rho. Da bi ugotovili razlike med kopijami genov za 16S rRNA pri sevu P. bryantii B|4 smo klonirali 5 od 7 kopij genov za 16S rRNA. Kopije so se razlikovale v o,3 do 1,2 % nukleotidov in ugotovili smo, da takšne razlike ne vplivajo na filogenetsko razvrstitev vrste. Z elektroforezo v utripajočem električnem polju smo ugotovili, da je genom P. bryantii B|4 velik približno 2600 do 2700 kb in potrdili da ima 7 operonov rRNA.Type of material - dissertation ; adult, seriousPublication and manufacture - Ljubljana : [M. Peterka], 2002Language - slovenianCOBISS.SI-ID - 117638912
Author
Peterka, Matjaž
Other authors
Avguštin, Gorazd
Topics
mikrobiologija |
vamp |
bakterije |
Prevotella ruminicola |
Prevotella bryantii |
molekularna genetika |
ribosomska RNA |
operoni |
promotor |
kloniranje |
genom |
velikost |
disertacije
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Library | Call number – location, accession no. ... | Copy status |
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National and University Library, Ljubljana | GS II 528342 glavno skladišče | available - reading room |
BF, Central Biotechnical Library, Lj. | Skladišče DR II 9206 IN: 02002056443 |
available - outside loan, loan period: 30 days |
BF, Dept. of Animal Science, Ljubljana | Naloge Dd PETERKA Opis lokusov IN: 120020190 |
available - reading room |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Peterka, Matjaž | 16327 |
Avguštin, Gorazd | 08320 |
Source: Personal bibliographies
and: SICRIS
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