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Študij ginogeneze in vloge mikoriznih gliv na rast in razvoj čebule (Allium cepa L.) z biotehnološkimi metodami : doktorska disertacija = Study of gynogenesis and the role of mycorrhizal fungi on growth and development of onion (Allium cepa L.) using biotechnological methods : dissertation thesisAngelova, ElizabetaV delu je bila proučevana primernost petih postopkov inokulacije različnih delov ženskega gametofita, s ciljem poenostavitve postopka ginogeneze in vpliv ter možnost uporabe kolhicina in orizalina ... pri zgodnji diploidizaciji genoma. Ugotovili smo, da je enostopenjska metoda inokulacije cvetov najmanj zahtevna ter dovolj učinkovita. Pri tej metodi smo dobili relativno velik odstotek homozigotnih regenerantov, in sicer skupno (4,52%) pri cv. belokranjka in pri cv. XPH 3371. Kombinacija rastlinskih hormonov 2,4-D in BA v indukcijskem gojišču se je izkazala kot najprimernejša. Kolhicin je imel zelo negativen vpliv na indukcijo homozigotnih regenerantov. Obe antimitotski sredstvi, uporabljeni v tej zgodnji fazi razvoja ginogenetskih regenerantov pretežno nista uspošno podvojili genoma. V sklopu raziskav vpliva interakcija arbuskularne mikorize ter njene odzivnosti na aplikacijo rastnega regulatorja jasmonske kisline smo proučevali dinamiko rasti in razvoja, dinamiko mikorizacije ter spremembe polipeptidov v listih čebule. Arbuskularno mikorizne glive so pozitivno vplivale na dolžino koreninskega sistema in na svežo maso korenin. S spremljanjem dinamike mikorizacije čebule z AM glivami smo dokazali vpliv jasmomske kisline na tvorbo posameznih struktur (hife, hife s arbuskuli in hife s arbuskuli in vezikli) simbiotske asociacije. S primerjavo elektroforetskih črt med vzorci proteinov ekstrahiranih iz mikoriznih rastlin in mikoriznih rastlin tretiranih z jasmonsko kislino smo ugotovili določene spremembe, povečala se je vsebnost polipeptidov z molekulsko maso 48 in 68 kDa.Type of material - dissertation ; adult, seriousPublication and manufacture - Ljubljana : [E. Angelova], 1997Language - slovenianCOBISS.SI-ID - 1517177
Author
Angelova, Elizabeta
Other authors
Gogala, Nada, 1937-2013 |
Bohanec, Borut
Topics
čebula |
žlahtnjenje rastlin |
tkivne kulture |
haploidi |
indukcija |
ginogeneza |
rastlinska genetika |
mikoriza |
glive, mikorizne |
jasmonska kislina |
disertacije
Library | Call number – location, accession no. ... | Copy status |
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National and University Library, Ljubljana | GS II 474568 glavno skladišče | available - reading room |
BF, Central Biotechnical Library, Lj. | Skladišče DR II 9071 IN: 01997055734 |
available - outside loan, loan period: 30 days |
BF, Dept. of Agronomy, Lj. | P Dr 326 1 | available - outside loan, loan period: 1 months |
BF, Dept. of Agronomy, Lj. | P Dr 326 | available - reading room |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Angelova, Elizabeta | |
Gogala, Nada, 1937-2013 | 00878 |
Bohanec, Borut | 09565 |
Source: Personal bibliographies
and: SICRIS
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